LOCUS       NZ_CP080314            11970 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP-426 plasmid p426-colRNAI, complete
            sequence.
ACCESSION   NZ_CP080314
VERSION     NZ_CP080314.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20373013
            Assembly: GCF_019434255.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Chen,J. and Cai,J.
  TITLE     In vivo emergence of colistin and tigecycline resistance in
            carbapenem- resistant hypervirulent Klebsiella pneumoniae during
            antibiotics treatment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Chen,J. and Cai,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUL-2021) Clinical Microbiology Laboratory, The Second
            Affiliated Hospital of Zhejiang University School of Medicine, 88
            Jiefang Rd, Hangzhou, Zhejiang Province 310009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v.0.4.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina NovaSeq 6000; Nanopore PromethION
            48
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:27:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,971
            CDSs (total)                      :: 5,850
            Genes (coding)                    :: 5,664
            CDSs (with protein)               :: 5,664
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 67 of 186
            Pseudo Genes (incomplete)         :: 124 of 186
            Pseudo Genes (internal stop)      :: 28 of 186
            Pseudo Genes (multiple problems)  :: 33 of 186
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080314.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP-426"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p426-colRNAI"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2020"
     gene            join(11241..11970,1..2)
                     /gene="mobC"
                     /locus_tag="KZ481_RS29530"
                     /old_locus_tag="KZ481_29535"
     CDS             join(11241..11970,1..2)
                     /gene="mobC"
                     /locus_tag="KZ481_RS29530"
                     /old_locus_tag="KZ481_29535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            9..539
                     /locus_tag="KZ481_RS29535"
                     /old_locus_tag="KZ481_29540"
     CDS             9..539
                     /locus_tag="KZ481_RS29535"
                     /old_locus_tag="KZ481_29540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(566..745)
                     /locus_tag="KZ481_RS29540"
                     /old_locus_tag="KZ481_29545"
     CDS             complement(566..745)
                     /locus_tag="KZ481_RS29540"
                     /old_locus_tag="KZ481_29545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(771..1199)
                     /locus_tag="KZ481_RS29545"
                     /old_locus_tag="KZ481_29550"
     CDS             complement(771..1199)
                     /locus_tag="KZ481_RS29545"
                     /old_locus_tag="KZ481_29550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(1363..1677)
                     /locus_tag="KZ481_RS29550"
                     /old_locus_tag="KZ481_29555"
     CDS             complement(1363..1677)
                     /locus_tag="KZ481_RS29550"
                     /old_locus_tag="KZ481_29555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1665..1943)
                     /locus_tag="KZ481_RS29555"
                     /old_locus_tag="KZ481_29560"
     CDS             complement(1665..1943)
                     /locus_tag="KZ481_RS29555"
                     /old_locus_tag="KZ481_29560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(2118..2483)
                     /locus_tag="KZ481_RS29945"
     CDS             complement(2118..2483)
                     /locus_tag="KZ481_RS29945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(2480..2851)
                     /gene="tolA"
                     /locus_tag="KZ481_RS29565"
                     /old_locus_tag="KZ481_29570"
     CDS             complement(2480..2851)
                     /gene="tolA"
                     /locus_tag="KZ481_RS29565"
                     /old_locus_tag="KZ481_29570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(3125..3370)
                     /locus_tag="KZ481_RS29570"
                     /old_locus_tag="KZ481_29575"
     CDS             complement(3125..3370)
                     /locus_tag="KZ481_RS29570"
                     /old_locus_tag="KZ481_29575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            4015..5502
                     /gene="ltrA"
                     /locus_tag="KZ481_RS29575"
                     /old_locus_tag="KZ481_29580"
     CDS             4015..5502
                     /gene="ltrA"
                     /locus_tag="KZ481_RS29575"
                     /old_locus_tag="KZ481_29580"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            5607..7292
                     /locus_tag="KZ481_RS29580"
                     /old_locus_tag="KZ481_29585"
     CDS             5607..7292
                     /locus_tag="KZ481_RS29580"
                     /old_locus_tag="KZ481_29585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            7302..7559
                     /locus_tag="KZ481_RS29585"
                     /old_locus_tag="KZ481_29590"
     CDS             7302..7559
                     /locus_tag="KZ481_RS29585"
                     /old_locus_tag="KZ481_29590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            7644..7793
                     /locus_tag="KZ481_RS29590"
                     /old_locus_tag="KZ481_29595"
     CDS             7644..7793
                     /locus_tag="KZ481_RS29590"
                     /old_locus_tag="KZ481_29595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            9277..11241
                     /locus_tag="KZ481_RS29595"
                     /old_locus_tag="KZ481_29600"
     CDS             9277..11241
                     /locus_tag="KZ481_RS29595"
                     /old_locus_tag="KZ481_29600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     BGC             607..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(7897..8475)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          11242..11969
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 gagggattat ggcgctgtac atggtattta ttgaggacgt ggaagacaaa gacggcgagc
       61 gctgcattat gccaaccagc gagcatctgg aagacatggt ggtgctcgcc tggcagaccg
      121 gggcgtatgt tttctgtttt gtcgaccggg cccagcatct ggcgatgatg aaatccatcg
      181 ggatgtatga cgagcggctc aacagcgacg aaaaagccac gtactttttc accggtgaaa
      241 atccgcaatg ggaacgggac aaacccacag gaatgaagct gtgtgattac ctctatcgaa
      301 aggtggagcg gttcgccatc gagaacggtg tgcaggtggt tgatgccggg aagtgctcac
      361 cggagcagat taacgcgatg attgaggtgg tcagcgagcg gcacggggaa agcgcggtga
      421 acgagaagct gaaagcggag ctgatgcggg aatataacag ccgctatttt aacgtgccgg
      481 tgcctgaact gccggaggac aaacccgccc gccggtggtg gccgttcggc aaaaaataac
      541 gccagcaccg gcatggccgg tgctgttatt cttccccgag cttatcccgc atcacctgcc
      601 agagtgtttc cgcatcctcg tgaagccgtt cgcacatgtc ggccggggta tccatgtcca
      661 gctcgttcag cttttccagc aacagcagcg actgatcctt aatgaattta gccatattga
      721 gggcagtttt ttcctgcttc gtcatgttct ccattccctt cggtaagttc tcagctaaac
      781 cagccggacc agaagcgttt tttcttctgt gtcagttctg ttgtcaatct gtccacttcg
      841 gcctggagtt tccggcgctc atcatcgtgc tggcggcgtt cttcttcccg ggatgtttta
      901 tcttccagca tcagggtgac agcctgccgg agatcggcca gttccttttg cattgccaga
      961 agctgttcct gtgggaaatt tttttgtgta ttttcacggt gtgaaatgcc gggaatttgt
     1021 ttctcaggga tattcacatt tccgtataca cggatcagtt ctgacacatc cacgacaggg
     1081 ttgtttttcc cgtcacgtga cacggtaacc tttccctgtt ttacatgatt gtatagcgtt
     1141 cttctggtaa tgcccgcggc ttgtgctgcc tgtgaaaggt tgagcaatgt tttcgccact
     1201 gtatacccct gattttatcg ggtgtgaaag gtgtgtaatt tctcacggta aatgtatcgc
     1261 tgaattttac ccgtttttcc gttcaggatg tggttttctg acgcgctgcg cttgtcaaac
     1321 cggcgacggc cacgcaatga attgcgcccc cgtcatcggt tattagaggc tgtctgcccc
     1381 cggatacccg gattctttct gatgcctgac ggtgacgatc acaatctggt ccatttcccg
     1441 gtcgtggcgg tagagcatca catagccact gtcgccaaac ccgatcacca gttccttata
     1501 ttccagcggc agaaacggca ccgggcggcc aatgtctggc aatgttttca gttgctggat
     1561 agcccggacg atcacctcac ctccttttct ggctgccagc cgatttttgg ttttgagaaa
     1621 gtcctgaagg cgttgtaaat cgcgctgtgc cagcgcggaa atcgttacct gtggcatggc
     1681 ggagcatcct gctcgttctc agttccccag gtacttatcc aggcttccgc ttcttccgca
     1741 gtaaggtgca gacccgtttc ctgatagtgc tgccatgcgg cctgtccgtc ccgcaggtac
     1801 tggctgcgct tctcttcccg gtcgatgtat tcggtgattg cctccagcat tagcgcgtgc
     1861 gctgagcggt gccggtcatc ggccagcgtt ttcagccggt ccttaagttc ttcatcgagc
     1921 ctgatggatg ttgctgatga cataaaagcc cctttgtagt catatgtatt acacatgact
     1981 actttaatcc gtggcggcgc tatttgccag aaatacggct tttgctgccc atttttcagc
     2041 ttcgcttcca ctaatcaggg ctgctacagg ctcactcatc atccaggact attgcagcct
     2101 gcgggttaaa ggacctgtta ctggcggcaa ccatcatcat tgagcttttg ggtggtgact
     2161 ggtcgcggat cctgtaagcc gtcatttttc agactgaatt caaaatttat aacctgtcgc
     2221 attgccgggt gccctgcggg atatagccag tgcctcattg ccgtcagcgt ttcccgaaaa
     2281 aaaaggtgac agccggtaag cgacacgttg tccactcgtc catcgtccag aatatcaaac
     2341 gtggcggtca cctgaccttc gatgcgtttt gctgcggcct gtaccgggta ggttggctgc
     2401 agtgtggtta cgggttttag tggtatcgca tgtaccggag tgaggaacat ggccgccagc
     2461 actgtcagag taaaagcttt catggtctga aatccagagg ggcgtttttg aatatttgcc
     2521 aggtattttc atccggtgcc ggcggaattt tggcgtgggt aacggctgat attgctgctt
     2581 tacagagttt cggatctcct ccttcagcaa tggcgctgtt cagagtgcca tcacgttgta
     2641 tggatatccg cacggtgcac atttttcccg cataggcctc tggttcaaac atctgcattt
     2701 caacagcggt cttgatggcc gcagcgtagc tactgatttt agatgactta tccagagtat
     2761 aaattttcgg cggctgggtg gtggaatgct tacaggccgt caggagtaag gggctgcaca
     2821 atgccagcag gataaagggt attaaacgca tcgggttgat gacctgtcag taaaaaatgt
     2881 tacatgtata tttgtcactc tttacggcca cgttcattta cataaacacg gcttatcgta
     2941 tcgtttttac accaaacggc ccgccatgca gttgcgtgca taggcatgca gcgggttaca
     3001 atttaccggg cgcgtttccg gcggttttcc gggtggtttg ttgcctgttt tatcccgtag
     3061 ccgccggaaa cgccctcaga ccgtctgagc ggtgcgggca atgcgacgtt atggtaaata
     3121 ccccttaccg gcggcgctgt ggctgccagg tgaaggtggt ttccgtagcc tcgtatttct
     3181 cccgacgcat ttcctgcgcc cgcctggctt cacgttcgtc ctgaatggca cgaagcaccc
     3241 tttcccgcac ctgttgtttt accgcctttg gcagcgtttt gttccctttc aggcccatgg
     3301 ccagtgcctg agcgacaaag gcgtccgcca ggcgcttttc ttcagcggga gtcagtttca
     3361 ggtgcgtcat agtggcctcc gggcgtctca ggtttcccga acagtttaac cgcaggtcct
     3421 ctcctcatgc cagttagctt tcagatgtgt ctataccatc tgaaatgtaa gtgtgatgaa
     3481 tgtcttaaat aataaaattt gttgccatga gaaattttcg ggaaaagacg cgatctgctt
     3541 acaggtggct ccgggcgctt ttttgtgcct gaagatgaga actaatctca tatattgaca
     3601 cctgaaaacg ggaggagtaa ggtaataatt atactgtgta tatgtacagt gtgcgccggg
     3661 agaccggtta gagatcaagg ggtgaaagtc cccgaccatt gaaggaccag caatccacaa
     3721 ggtccccgag tcatgcgttg cataccgcga ggtatggggc gaagcgttga caggggtgtt
     3781 gacaggccag ccattgagcc acgaaatgta tattaaatta ccgggtgccg acgttgtact
     3841 gttaacggaa ggcaacatca tagggtgcga cactgcgagt gccacatgga cccggcgggg
     3901 tctgagaccc tggcatgtca atacgatctc tacgcgggaa ccgggagatc tcccctctga
     3961 ccatctgcca gtgtcggaga tggcccgcac cgggaagacg aggagtcata gccggtgatg
     4021 tacggagagg agaagtcgga ctcgctcata gtagcggcga agcaggcgaa caacccgaaa
     4081 ggagcggagt cagtggagcg aaggagcggg gccaagggga acgcggaaca gccacacatg
     4141 cgccggacac agagccggga aagcatgtca cagaggctgt cacgcgtgcg ggaagctgcg
     4201 aagcagcgga agaaagaacg gtttacagca ttgttccacc tgctgacagt cgaagcactg
     4261 gaagccgcat tcctctccct gagcaggaaa gcggccgccg gagtggatgg catcaggtgg
     4321 atggactacg ccggaaacat gaagaacaac ataacagatc tgcaccggag gctacatcag
     4381 ggcagctaca gggcgcagcc cggcaggcgt cactacatcc caaaagcgga tggaaaacaa
     4441 cgcccgctcg gcatcgcctc gctggaggac aagatcgtcc agtatgcgct ggtgaaaatc
     4501 ctgaacgcag tctatgaaaa cgactttatg gggttctcat acgggttcag acccgggcga
     4561 agccagcacg atgcactgga cgcactggcc acagggctgg tacgcactaa cgtaaactgg
     4621 gtactggatg ccgacatcag tcagttcttc gacagggtga gccacgaatg gctgatcagg
     4681 ttcacagagc atcggatcgg cgaccggagg gtaatcaggc tcatacgtaa gtggctcaca
     4741 gccgggacgt cggaggaggg tcaatggcga gcaacggagg aaggcacccc acagggtgcg
     4801 gtcatctcac cgctgctggc aaacatatac ctccactacg tcttcgatct gtgggcgcat
     4861 cagtggcgac gtcgctatgc cacaggcaat gtggtaatgg tcagatacgc cgatgacatc
     4921 gtcatcgggt tcgacaaacg atacgatgcc cggcgcttcc gtatagccat gcagcgcaga
     4981 ctgagggagt tcggactcac ggttcacccg gagaaaaccc gtctgatgga gttcggccgc
     5041 ttcgctgccg aaaaccgtgc catcagggga aaaggcaaac cagaaacgtt caacttcctc
     5101 gggttcacgc acatcagcgg gaaagatcgc aacggcaggt tcatgctgat acgaaagacc
     5161 cgccgggatc ggatgacggc aactctgaaa gccatcaaag acggtctgcg aaggcgctgg
     5221 cattactcaa tccccgaaca gggaaaatgg ctcaggagag tggttcaggg atacctgaac
     5281 tatcactcgg taccgggcaa cttccccacc atgcagaagt tcaggacaca cgtaacaaac
     5341 ctctggcgcc gggcgctcag gcgcaggagc cagaaggatg atacgacctg gacgaaagca
     5401 aacaaactgg cagccgcatg gctaccaagg gttcgggttc ttcatccatg gcctgtggag
     5461 cggttcaccg ccagacaccc gaggcaggag cccggtgcgt aaatcgcgca cgccgggatc
     5521 tgtgcggggg gtatccggta acgggtatcc ctaccgcgac atgttggctt taatttattg
     5581 ttttaaatgt caaagaggaa aacggtatga gtggtggaga cggtcgaggt ccgggtaatt
     5641 caggtctggg acataatggt ggtcaggcca gtgggaatgt gaacggtacg tctggtaaag
     5701 gtggcccttc atcaggtggt ggtacggatc caaacagcgg gccgggctgg ggtacgacgc
     5761 atacgccgaa tggagatatt cataactata atccggggga gtttggtaac ggcgggagta
     5821 aacccggtgg taatggtggt aacagcggca atcatagcgg tagctctggt ggcggacagt
     5881 cttcggccac tgcgatggcc ttcggtctgc ctgccctggc tactccgggg gctgaaggac
     5941 tggctttatc agtttccggc gatgcgttgt cggccgccgt tgctgatgtg ctggctgccc
     6001 tgaaggggcc gtttaagttt ggtctgtggg ggattgcgat ctacggtgtg ctgccttctg
     6061 agattgcaaa agatgatccg aaaatgatgt caaaaattat gacgtcatta ccggccgatg
     6121 cggtgacgga gactccggca agtactttac cactggacca ggcgacggtt cgtgtcagac
     6181 aacgggttgt ggatgtggtg aaggatgagc ggcagcatat tgcggttgtc gcaggtcggc
     6241 caatgagtgt ccctgtggtg gatgcgaaac cgacaaaacg tccgggggta ttcagtgtgt
     6301 cgattccggg tctcccgtct ctgcaggtga gcgtaccgaa aggtgttccg acagcgaaag
     6361 ccccgccaaa aggcattgtt gctgaaaaag gtgattcacg tccggctggt tttacagccg
     6421 gtggtaactc ccgtgaggcc gttattcgtt tcccgaaaga gaccggacag aagccggttt
     6481 atgtgtcggt gacagatgtt cttaccccgg cacaggtaaa acagcgtcag gaggaagaaa
     6541 agcgtcgcca gcaggcatgg gacgccgctc atccggaaga ggggctgaaa agagactatg
     6601 ataaagcgaa agctgagctg gatgccgaag ataaaaatat tgcgacctta aacagccgca
     6661 ttgcatcgac agagaaggcg ctccccggtg caagggctgc tgttcaggaa gccgataaaa
     6721 aggtaaaaga ggcagaggca aataaagatg attttgtgac ttataacccg cctcatgaat
     6781 atggctccgg gtggcaggat caggttcgct atcttgataa ggatattcag aatcagaatg
     6841 agaaattaaa agcggctcag gcatctttaa acgcaatgaa tgaatcctta tccagagata
     6901 aggctgcact taccggggcg atggagagcc ggaaacaaaa ggagaaaaaa gcgaaggatg
     6961 cagaaaataa gttaaatgag gaaaagaaca agcctcgcaa ggggactaaa gactacggcc
     7021 atgattatca tccagtacct aaaacagaag acataaaggg acttggagag ttaaagcgag
     7081 gtgatcctaa aacacctaaa caaggtggtg gtggtaagcg ggcccgatgg tatggcgata
     7141 aaaaacgtaa aatttatgaa tgggattccc agcacggtga gcttgaaggg tatcgtgcca
     7201 gtgatggcga acacctaggc gcatttgatc ccaaaacggg taagcaggtt aaagggccag
     7261 atccaaaacg gaacattaaa aaatatcttt gagaggtaaa tatggggctt aaattacgtt
     7321 tgacatggtt tgataagaaa actgaagagt ttaaagggga agagtattct aaagactttg
     7381 gtgacgatgg ttctgtcatt gaaagcttgg gaatgccttt aaaggataat attaataatg
     7441 gtgagtttga tttgaagaaa gaatgggttc ctttactaca gccatttttt aaaaatcaaa
     7501 taaaacctga tgtggataat tgttatatag cttttgatta tcgggacggt aactggtaac
     7561 aaaaaattat caaggatgat ttgaaatact gacttccggt gttcagggat gaatgccgga
     7621 gtctttaaat acagggaaag tatatgaaaa aggtaaagac tatttttctt tttatattga
     7681 ttgttgctgg ctttcttctg gccgcatgtc aggcaaatta tatccgggat gttcagggcg
     7741 gaacggtggc accatcgtcc tcctctgaac tgacggggat cgcggttcag tagaaaagat
     7801 caaaggatct tcttgagatc ctttttttct gcgcgtaatc ttttgccctg taaacgaaaa
     7861 aaccacctgg ggaggtggtt tgatcgaagg ttaagtcagt tggggaactg cttaaccgtg
     7921 gtaactggct ttcgcagagc acagcaacca aatctgtcct tccagtgtag ccggactttg
     7981 gcgcacactt caagagcaac cgcgtgttta gctaaacaaa tcctctgcga actcccagtt
     8041 accaatggct gctgccagtg gcgttttgcg tgctttcccg ggttggactc aagtgaacag
     8101 ttaccggata aggcgcagca gtcgggctga acggggagtt cttgcttaca gcccagcttg
     8161 gagcgaacga cctacaccga gccgagatac cagtgtgtga gctatgagaa agcgccacac
     8221 ttcccgcaag ggagaacggc ggacaggtat ccggtaaacg gcagggtcgg aacaggagag
     8281 cgcaagaggg agcgacctac cggaaacggt ggggatcttt aagtcctgtc gggtttcgcc
     8341 cgtactgtca gattcatggt tgagcctcac ggctcccaca gatgcaccga aaaagcgtct
     8401 gtttatgtga actctggcag gagggcggag cctatggaaa aacgccaccg gcgcggccct
     8461 gccgttttgc ctcacatgtt agtcccctgc ttatccacgg aatctgtggg taactttgta
     8521 tgtgtccgca gcgcccgccg cagtctcacg cccggagcgt agcgaccgag tgagcgagga
     8581 ggcgcaattt ccccggcacc tgcaagacgg tcaccggacg cgctgatgtg ggatattgtc
     8641 ccctgattaa gtctttctat tccctgacga tcaattatca ggataggtga accatgtcgg
     8701 tttttcgctg aacattttac gttaacctat ctctgttgct tttttctcct tgcggagaaa
     8761 tgcaagtcat ccccgctggg gcgtggctga cttcagggga aaagaccttg cttacgctgg
     8821 ctggacgcca ccgacgcacg gtcttttccc ctctttctgg ctctgtccgg ctggacgccg
     8881 gccacgccag aaattcaccc ggcccccgtc aggctcacgc ctgaccaccc acaaaaatcc
     8941 acggtgctgg acgcacctga cgaagcagca aagctgctcc tgattttgcg ggtggtcagc
     9001 cgctcgctat ggcgaactct gacgggggcc ggaacaccgg aaaagaccgc actggggtgc
     9061 cgccttttcc tctcacacgc tggggcgtgc tcgttgttcc tccggggctg cgccttttcc
     9121 gcatggggcg gaaagaggct ggcctccgcg gtgaaagctg aacgcaatca ccacggacgt
     9181 gaccgccgcc gccatgcctt ggggcatgtc cgcggcttgt gcagcggggg ctgcgcatca
     9241 cgccggtaaa cccgtcgtct gaaggagaga gattgcatgt ttaatacgga ttcgcttgcc
     9301 tggcagtggc atatcccgga gctggcccgg cagatttacc cgtgggtcag ctggctgtac
     9361 cgggagtatt acagtctggc cggttttttt ctggttatcg gcctgttttt cggtctggct
     9421 gaactctggg ccggccgaat gcgttttgtg gggctgagcc ataaggcgcg gcgggagtat
     9481 gccgatcgct ataagcgcga taataaccgg actgtgccat tttcggtgct ggcctcgctg
     9541 gattcacccg ggcggatggt cagggcacgg cgacatgtct ggaactacgc gtactggctg
     9601 accggcgctg ccggtctggc ccttctgggg gggctggttg tcgccgtgtt tatcgagagc
     9661 tggggagaaa ccgtcctgcg cccggacctc tatgcgaacg cagattatta cctgctgcag
     9721 ccggctaacc tgttcgggat agcctggtgt gccggagggc ttgtcgccgg cgtccttctg
     9781 gcgcggctgc tggcgttcct gctgcttgat ggtcattttg cggccgctga cgaggcggtg
     9841 aacgcgaaac tgaatcagga gagccggcgc agcagccagc ggaccggaga aatgacagac
     9901 gtccgccacc tgcatttcgg ggagccggta ccggttaacg cgctggccga tttttccacg
     9961 gagcaggccc ggaaacagca ggcggtgttt ctgggtaagg atgagcaggg ccagccggtg
    10021 ctggtccctc gtgatacctg gcgcaagacc aatattcaga tcctcggcct gcccgggagc
    10081 ggcaagagcg cgatggccac caacgcgctc attcgctgcg tacgcgattt cggggacgct
    10141 gtggtgtact tcgaccccaa aggggacgcg tgggcgccgc acgtcttccg ggcgcactgc
    10201 ccggacttca ccctgctgga tctgcggccg gggaaaccgg cgcagctgaa tctgttccgc
    10261 gatctcgacc agtacgccct gaagaatctg ctggttgccg ggtttaacct gagtgagacc
    10321 ggcgacgtgg cggaccacta ccgcatcagc gagcagaaag cggcgaagct gattgcggag
    10381 cagttcccgc agggggcaaa cattcagcag gtgctggcgg cggcgtacgc gttgccggag
    10441 gccctgaaaa aggacgtgaa ggggctgatc accaagctgg agaacgtggc cgacctgagc
    10501 gtcctgcaga cggacagcgg tatcgatgtg gcggggataa tcaacggcgg cggctgcctg
    10561 tacgtcatcg gctcgatgga cgatgaggcg gtgatccggg tgcagaagat gctgtttgcc
    10621 cgctgcgcgc aaatcatcat tgcgcgggat gagttccgga ggtggccgca cgcgagcatc
    10681 atgctcgatg agattaaata tctgctgtcg aagtacgtgc tgaacgcgct ggggacgctg
    10741 cgcagccggg actgcaacct gctgctcgcg caccagtcgc tgggcgactt cggtcagtgc
    10801 gggcaggatc tgcccgctga ttttgtgaaa accacggtac tggataacac gccgatccgc
    10861 tggttctatc gcgcggccag ccaggagtcc gcgcagtggg cggcgggcca gaccggggag
    10921 atccgggtgg acgtggagcg ccgccgggcc agccgggagg cggggaacgt ggagcacatc
    10981 agcggcgaca gcttcatcca gaaggaggcg cggccgctgt ttgacgtgaa caccctgcaa
    11041 cacctgcctg acgggttcgc ggtgatgacc gggctgggcg tggcgcggct ggcgttcagc
    11101 agcccgctgc gggttgagcg ccgggaaatt tcgctgaaaa ccttcccggt gctggcgaaa
    11161 acggacccgc tggcggagta cctgcctccg gccgacggcg gcggaccaaa agcggatgat
    11221 gattttgcgg ggctttactg atgacactgg agcagtacaa cgtcagccac gcgaagcggc
    11281 aggcgcggaa cgcggagaaa acacggctga ccctgcgctg gctgcgggaa gagctgtgtt
    11341 ccacggcgga gctggttgcg cgacggctgg ggattgcggc ggtacagccg gtttaccgct
    11401 tcctggacag tctggtggcg aaagggctgc tggtccgggc gaaatacccg gttgacggcc
    11461 gtcaggtcag cgtgtggggg ctgacgccgc acggggtggc gttctcgttc gacgaggacg
    11521 agccgctgac ggacgttatt ccgtttcagc cgtcaagggt gtcggcggcg cagctgccgc
    11581 accggctggc cgtgcagtcc ctgcgcctct ccatggaggg ccggggggcc accggctggc
    11641 ggtacctgca ccgtatggcc ctgaaaggga tgaaagtgcc ggacgcgctg gcggagctgg
    11701 acggcagaac ggtggcattt gaggtagagc gcaccgtcaa gagccgcagg cggtatcagg
    11761 aagtggtgtc cgggtatctg ttcaaccgcc gggcgaacgg catcgatgaa atctggtaca
    11821 tctgccctga ccggccgacg cttgtccggg tacagcgggc gattctggcg gtggatgaaa
    11881 tcgtgaatcc acagaccggc gaggcccgca aaacggtgga gctggaccgg gagaggctgt
    11941 tcgcgtgctt taaatttatg acaacggagt
//
