LOCUS       NZ_CP080308             5596 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP-CT77 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP080308
VERSION     NZ_CP080308.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20375149
            Assembly: GCF_019434295.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Chen,J. and Cai,J.
  TITLE     In vivo emergence of colistin and tigecycline resistance in
            carbapenem-resistant hypervirulent Klebsiella pneumoniae during
            antibiotics treatment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Chen,J. and Cai,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUL-2021) Clinical Microbiology Laboratory, The Second
            Affiliated Hospital of Zhejiang University School of Medicine, 88
            Jiefang Rd, Hangzhou, Zhejiang Province 310009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v.0.4.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina NovaSeq 6000; Nanopore PromethION
            48
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:28:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,967
            CDSs (total)                      :: 5,846
            Genes (coding)                    :: 5,664
            CDSs (with protein)               :: 5,664
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 67 of 182
            Pseudo Genes (incomplete)         :: 122 of 182
            Pseudo Genes (internal stop)      :: 25 of 182
            Pseudo Genes (multiple problems)  :: 32 of 182
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080308.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP-CT77"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed2"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2020"
     gene            205..363
                     /locus_tag="K0E63_RS30145"
     CDS             205..363
                     /locus_tag="K0E63_RS30145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            721..1047
                     /locus_tag="K0E63_RS29595"
                     /old_locus_tag="K0E63_29595"
     CDS             721..1047
                     /locus_tag="K0E63_RS29595"
                     /old_locus_tag="K0E63_29595"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            1073..1618
                     /locus_tag="K0E63_RS29600"
                     /old_locus_tag="K0E63_29600"
     CDS             1073..1618
                     /locus_tag="K0E63_RS29600"
                     /old_locus_tag="K0E63_29600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            1684..2034
                     /locus_tag="K0E63_RS29605"
                     /old_locus_tag="K0E63_29605"
     CDS             1684..2034
                     /locus_tag="K0E63_RS29605"
                     /old_locus_tag="K0E63_29605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2104..2370)
                     /locus_tag="K0E63_RS29610"
                     /old_locus_tag="K0E63_29610"
     CDS             complement(2104..2370)
                     /locus_tag="K0E63_RS29610"
                     /old_locus_tag="K0E63_29610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2354..2578)
                     /locus_tag="K0E63_RS29615"
                     /old_locus_tag="K0E63_29615"
     CDS             complement(2354..2578)
                     /locus_tag="K0E63_RS29615"
                     /old_locus_tag="K0E63_29615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(3245..3457)
                     /locus_tag="K0E63_RS29620"
                     /old_locus_tag="K0E63_29620"
     CDS             complement(3245..3457)
                     /locus_tag="K0E63_RS29620"
                     /old_locus_tag="K0E63_29620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3464..3973)
                     /locus_tag="K0E63_RS29625"
                     /old_locus_tag="K0E63_29625"
     CDS             complement(3464..3973)
                     /locus_tag="K0E63_RS29625"
                     /old_locus_tag="K0E63_29625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4008..4712)
                     /locus_tag="K0E63_RS30150"
                     /old_locus_tag="K0E63_29630"
                     /pseudo=""
     CDS             complement(<4008..4712)
                     /locus_tag="K0E63_RS30150"
                     /old_locus_tag="K0E63_29630"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4651..4995)
                     /locus_tag="K0E63_RS29635"
                     /old_locus_tag="K0E63_29635"
     CDS             complement(4651..4995)
                     /locus_tag="K0E63_RS29635"
                     /old_locus_tag="K0E63_29635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(4985..5161)
                     /locus_tag="K0E63_RS29640"
                     /old_locus_tag="K0E63_29640"
     CDS             complement(4985..5161)
                     /locus_tag="K0E63_RS29640"
                     /old_locus_tag="K0E63_29640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     Typing          complement(5248..5324)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          complement(94..274)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.956"
                     /coverage="90.0"
                     /evalue="3.08e-71"
     Typing          complement(3130..4661)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcagaggagg acgaaacccg acaggactta aagatgccgg tgatttctct ccggcggctc
       61 cctcgtgcgc tctcctgttc cgaccctgcc gtttaccgga tacctgttcc gcctttttcc
      121 ctttcgggaa gcgtggcgct ttctcatagc tcacgctgct ggtatctcgg ctcggtttag
      181 gtcgttcgct ccaagctggg ctgtatgcac gaaccccccg ttcagcccga ctgctgcgcc
      241 ctttccggta actatcatct tgagtccaac ccggacggac acgcaaaaac gccactggca
      301 gcagccattg gtaactggat atatacgaga gagatattga gaggtcttga agtgggtgcc
      361 taactgcggc tacactgaaa gaacagtttt ggtatctgcg gtctcgtata cccagttacc
      421 cggttaagca gttccccaac tgacttaacc ttcgatcaaa ccgcctcccc aggcggtttt
      481 ttcgtttaca gtgcaagaga ttacgcgcag aaaaaaagga tctcaagaag atcctttaaa
      541 tgatcgtcag ttctgttatc cccggagctc catccatccc acggaaaaac acccgccact
      601 gatgccctca gcgcactgca catcatgtac aaaccactcc ctccctcctt ttttattgcg
      661 tttcttaatg cgcaatggta gctttgtgcg caacaaaaaa cgcaatagca agggggggta
      721 atgagagaaa gaacaaatat catgctagat ataggattaa agcaatcctt acaacggctt
      781 tcgaataaaa ccaaaatgag tctaagtgat cttattaacc agagattatc aggttcactt
      841 tcagttgaag aacgcattgg cgaaaagcct gaatggatga gaaaccttga tccctatctc
      901 gccaatcact tttcaaatat tgagttaccg ctcccttcac cattaaaaga ataccaagaa
      961 aaaatggatg agatagattc aattattaat gacatgatgg ttctaaagga atatttcaga
     1021 gctaagtttt acgacgaaat aaaataggca cgctatacgg aggcggagaa ttatgttttt
     1081 taaacggtat ttaacggaga aggaaatata caaggcagca ctcccaattg ttaggcttca
     1141 agggagagtt aacacttcat tacgtttttt atccaaatac tcagatgtaa acaaaaatgg
     1201 attaattgca ttgcatctta atttattaca ttttatctca tacaacatac acggtgtaga
     1261 gtctctgaaa atcactcaag caggcataaa caaagctgta caacaaagcc ctcacgcaga
     1321 tgaaataaaa aatattgcgc aggcaatagg acgcaaagga cctgagtaca tcgaaggatt
     1381 aattagaaac ccatggggga agaaaaatga taatccacta atatttataa tgagcattga
     1441 agaaagtata aaagaaaccc ttaactcagg ttatatgtta tatagagtag ctcccataac
     1501 tgagggtgaa gcagctgctt tcatggatgt tcttaaacaa ttattattat ttgaggataa
     1561 ttttcgcctt tttggtagag acttggttta taaaatagaa aaattaaaaa tcagataaca
     1621 aagtatggtg aacgaaaaat gaaagagagg atagtgaaat gtgtaatacc aagattctca
     1681 actatgtatt ggttaattgc gttcctagca tttatattcc ccggtcgatc ttttgctgac
     1741 ggttacaccg cttactaccg atgtaatggt actcaggaag catctcttac tgttaaagat
     1801 aacaaactaa gtatggttgt aggtctttct aagtttaata ggcaactgga tacttataga
     1861 ttcaaagaaa agggaatcga agtagtaatg cagagagctg cttctgatga taactctgag
     1921 gccataatgt cttttattat gcctgacgtt aacaatatga ccttattagt taaaaataac
     1981 ggctacatta tcgttaagac aacctgctgg ttaactaaaa ccgacaaaca gtagaacacg
     2041 tagttagtcg agtatttacc ataatacctg ttgtcggcat caaaaaagcc cgcatagcgg
     2101 gcttcagtcg taaacttcgc ggcgatgtcc aatcgtggcg gcaaggataa caagctcgtc
     2161 atctcggatc tcacacagaa cgcgataatc tcccacccga tagcgccaga actcgcccag
     2221 ctcacctttg agcggcttcc ctgactggcg aggatcggca gcaactgcaa tgcgtaaact
     2281 cataaaatcc acaatccgtc gtgcgttctg tttgtccatc ttgcgtaacg atttgagcgc
     2341 ccgatcggaa tagttaatcg tccagaccaa gtgacttcct cacgtcttca gacgaatgca
     2401 ctgcttcatc accacggcga acgcgtgcag cagtttctgc tgaaagataa taatcctcca
     2461 ggtcttccag atgcgccagt attgcttccc gcgcataaaa cgtctttgtt ctgccggttt
     2521 gcttcgccag cgagtccaga cgggactcaa tctcatcaga taaccggata gcaagcatat
     2581 aaacctcccg aacaccattt gctatacatg tatatcaaca gtaccacaat caacctaaaa
     2641 gaacaaaaaa taatcttgtt agcccctctt gataatccgt aacgcggtca atatatctag
     2701 cttgagggct ttcctcttaa ctattgaaag gataacgatt atgggtctta agccgggtcc
     2761 gaaacgaatt gccgattcaa caggggaacc tgataaacgc cagcgtgaca ataaaaaaac
     2821 gccgggaaat actgacaaat tgaaacccag caaatcttca aaaaaataag tcagtagtta
     2881 aaagccctcc ggggcttttt tgtatctgcc gttcacgctg attaaacata acacccgttg
     2941 cccgcaccgc cccggacggg ttcgccgcga acggggtggc agtcaggtaa aaccggcaac
     3001 aaaccacccg ataaaccgcc ccgttcgcga cagataaatt ttacctggct gcatagccat
     3061 gcagccaggt gaatgacatc cggtttcagc gtaaatgccc gatgagaacc ctgttttcct
     3121 gcatttcagc ccagcgacgg accgtcatag cttcgctgcc gggaacgcgc ttccgtcagt
     3181 tccttttcgt gctgccgttg ctcgtgcgcc cgttctgcgg ccacggccat ctcatgacgc
     3241 tgcactaccg gctcaaacgc tgcagttgcc cgctcaagcg ttccacctgc tgactcaagc
     3301 gcgtcacgcg ctcgctcagc gccgcgttct cccgttgcat aagccctgac attttccgcc
     3361 attccccgaa ggcgctctcc cactcgtcca gccttttcga gtagtcctgc tgtagctgct
     3421 ctaatgcgct cagcaactgt ttttccagct cggtcatcgg cacttactcc ttcaccagtc
     3481 tcagcgcgtt ctgcttcgac cgcccgttcc cgaccgtcca gctctgcgtc ctgtctacgg
     3541 ccgtcccctt cggcagcacc agaaaacgtc cgttgctgtc ttccaggtac gtcacgcccc
     3601 aggtcttcgc gttcagcttc gacagcgctg cgttctgccg gtcgatttcg gccagatttg
     3661 acgctatcaa gctcccctga taccacaaca ccccgctcag gctggcgaac agcagtcctg
     3721 acaccatcgt gatggtcagc catgtccgcc cgaccatacg catcacgctc aggcggttcg
     3781 actgcatggc cgcgctcata ccctgctcgt gggcgcggat ggcggcgctg attctcttcc
     3841 cgctttcgct cagttccgct ctgacagact tctcgtgctc gctgaatgcg gctttcagca
     3901 tttcgccggt actctgctgc tgcgctttcg atttctgctc taagtccttc gccagcgtta
     3961 aaagactgtt catagatggc tcccttaagt cggatgtttc gccctccgtc cgggtcggca
     4021 atgctgatgc tgctctttgt ggtgcgcacg acctcaaaac ctgcgctttc cagcgcctca
     4081 gtgacgtcct gacgcgtttt aagctcccct gacgaggcga gggccagtaa gccccgcgta
     4141 atagcctctg cggcttcctg cttcgttttg ggtaacgaag atggcgtcac cagcgcgcgc
     4201 cggttctccg gtgcgttcgg gtcgtgcagc cccagtcgcc cgttcaccac ggtctgccac
     4261 gcatcgatgc gcggacggtc tgcccggtcg tagtacggct ggagacgcct gccggtcagc
     4321 agttccgtgt tcggtaccag aaaattcagt tccagccgcc ccttgtcccg gtgttcaacc
     4381 cacagcacgc tgtactggtc tttatcgagt ccgggcatca gaacccgctc gaagctcgcc
     4441 atcagctttt cacgctggcc gggcggtaaa tcctgttcag caaaagacag gacgccggag
     4501 gtgtactttt tggcgtaggg cgaggcgtcg ataagctccc ggacctcctc cggctttccc
     4561 tgcaggacga tggccccgtc gcgctggcgg tctttcccca gcagataatc caccggcccg
     4621 gcaccgccgc ctcgccctcg cggatgaaac ttaacgatca tcgtctgccc ccttttccag
     4681 cacggcatgc cgcagccgct cgagttcggc atcaatggcc atcagcgcgg ccaccacctg
     4741 aacccggtca gcgcctgacc actggccgcc gttaattttc cgggcgatct ggttgaggtt
     4801 gttccccatc ccggcaagct ggcgcagcaa aaccggatcg atggacggca gccgcaacga
     4861 ccgcgccggg cgcgtatcga gacaggtttc ccgcatccac accgccagtt gccttccatt
     4921 gcagcgctcc agcagctgct ggtactcccc atcggtcacc cggataccta aaaaacgggt
     4981 acgcttaacc gccatcctct cctccgatat ccgcaaaatg tgaccgggct gaacgctcaa
     5041 gctctgccat ctgctcccgg cggtaatctt cctcctgtgc ctgacgcatc agatttgcca
     5101 tcactgccgg atgccaggat ctttttttta cgacgagcgg cttcttcttc ggcttctgca
     5161 ttatccctcc ttcgcaaacg ccggacggca cggaaaagtt ttccgggtcg ctccggcgtt
     5221 ggacaagcgc cagcgcgtca gtggtgttag cgggtttcgg ggcgcagccc tgaaccagtc
     5281 acagcgttta cgctgtgtat actggcttac tatgttggca tgagagcacg attcatgaaa
     5341 gtgcctcatg tggcaggtgt gatatgtggc tcaggagcgt ccgcaatacc atgcacggac
     5401 agatgaaatg acgcctcctc gctcactcgg tagctgcgct cctgccgttc ggctgcggcg
     5461 ggcgtgaccg actcacggat aacgggataa agcaaaggcg ctcaaatcag aaagggccgg
     5521 agaaaaggat gcggcgttgc cgtttttcca taggctccgc ctcccctgac gagcatcaca
     5581 gaaatggacg ctcaag
//
