LOCUS       NZ_CP080302             5596 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP-C76 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP080302
VERSION     NZ_CP080302.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20373014
            Assembly: GCF_019434275.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Chen,J. and Cai,J.
  TITLE     In vivo emergence of colistin and tigecycline resistance in
            carbapenem- resistant hypervirulent Klebsiella pneumoniae during
            antibiotics treatment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Chen,J. and Cai,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUL-2021) Clinical Microbiology Laboratory, The Second
            Affiliated Hospital of Zhejiang University School of Medicine, 88
            Jiefang Rd, Hangzhou, Zhejiang Province 310009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v.0.4.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina NovaSeq 6000; Nanopore PromethION
            48
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:41:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,931
            CDSs (total)                      :: 5,810
            Genes (coding)                    :: 5,631
            CDSs (with protein)               :: 5,631
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 67 of 179
            Pseudo Genes (incomplete)         :: 119 of 179
            Pseudo Genes (internal stop)      :: 26 of 179
            Pseudo Genes (multiple problems)  :: 33 of 179
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080302.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP-C76"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed1"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2020"
     gene            join(5426..5596,1..174)
                     /locus_tag="K0E64_RS29420"
                     /old_locus_tag="K0E64_29420"
     CDS             join(5426..5596,1..174)
                     /locus_tag="K0E64_RS29420"
                     /old_locus_tag="K0E64_29420"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            383..>817
                     /locus_tag="K0E64_RS29825"
                     /pseudo=""
     CDS             383..>817
                     /locus_tag="K0E64_RS29825"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            852..1361
                     /locus_tag="K0E64_RS29430"
                     /old_locus_tag="K0E64_29430"
     CDS             852..1361
                     /locus_tag="K0E64_RS29430"
                     /old_locus_tag="K0E64_29430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            1368..1580
                     /locus_tag="K0E64_RS29435"
                     /old_locus_tag="K0E64_29435"
     CDS             1368..1580
                     /locus_tag="K0E64_RS29435"
                     /old_locus_tag="K0E64_29435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2247..2471
                     /locus_tag="K0E64_RS29440"
                     /old_locus_tag="K0E64_29440"
     CDS             2247..2471
                     /locus_tag="K0E64_RS29440"
                     /old_locus_tag="K0E64_29440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            2455..2721
                     /locus_tag="K0E64_RS29445"
                     /old_locus_tag="K0E64_29445"
     CDS             2455..2721
                     /locus_tag="K0E64_RS29445"
                     /old_locus_tag="K0E64_29445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2791..3141)
                     /locus_tag="K0E64_RS29450"
                     /old_locus_tag="K0E64_29450"
     CDS             complement(2791..3141)
                     /locus_tag="K0E64_RS29450"
                     /old_locus_tag="K0E64_29450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(3207..3752)
                     /locus_tag="K0E64_RS29455"
                     /old_locus_tag="K0E64_29455"
     CDS             complement(3207..3752)
                     /locus_tag="K0E64_RS29455"
                     /old_locus_tag="K0E64_29455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(3778..4104)
                     /locus_tag="K0E64_RS29460"
                     /old_locus_tag="K0E64_29460"
     CDS             complement(3778..4104)
                     /locus_tag="K0E64_RS29460"
                     /old_locus_tag="K0E64_29460"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(4462..4620)
                     /locus_tag="K0E64_RS29945"
     CDS             complement(4462..4620)
                     /locus_tag="K0E64_RS29945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            5260..5436
                     /locus_tag="K0E64_RS29465"
                     /old_locus_tag="K0E64_29465"
     CDS             5260..5436
                     /locus_tag="K0E64_RS29465"
                     /old_locus_tag="K0E64_29465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     Typing          5095..5174
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(4512..4909)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          165..1696
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgccagcttg ccgggatggg gaacaacctc aaccagatcg cccggaaaat taacggcggc
       61 cagtggtcag gcgctgaccg ggttcaggtg gtggccgcgc tgatggccat tgatgccgaa
      121 ctcgagcggc tgcggcatgc cgtgctggaa aagggggcag acgatgatcg ttaagtttca
      181 tccgcgaggg cgaggcggcg gtgccgggcc ggtggattat ctgctgggga aagaccgcca
      241 gcgcgacggg gccatcgtcc tgcagggaaa gccggaggag gtccgggagc ttatcgacgc
      301 ctcgccctac gccaaaaagt acacctccgg cgtcctgtct tttgctgaac aggatttacc
      361 gcccggccag cgtgaaaagc tgatggcgag cttcgagcgg gttctgatgc ccggactcga
      421 taaagaccag tacagcgtgc tgtgggttga acaccgggac aaggggcggc tggaactgaa
      481 ttttctggta ccgaacacgg aactgctgac cggcaggcgt ctccagccgt actacgaccg
      541 ggcagaccgt ccgcgcatcg atgcgtggca gaccgtggtg aacgggcgac tggggctgca
      601 cgacccgaac gcaccggaga accggcgcgc gctggtgacg ccatcttcgt tacccaaaac
      661 gaagcaggaa gccgcagagg ctattacgcg gggcttactg gccctcgcct cgtcagggga
      721 gcttaaaacg cgtcaggacg tcactgaggc gctggaaagc gcaggttttg aggtcgtgcg
      781 caccacaaag agcagcatca gcattgccga cccggacgga gggcgaaaca tccgacttaa
      841 gggagccatc tatgaacagt cttttaacgc tggcgaagga cttagagcag aaatcgaaag
      901 cgcagcagca gagtaccggc gaaatgctga aagccgcatt cagcgagcac gagaagtctg
      961 tcagagcgga actgagcgaa agcgggaaga gaatcagcgc cgccatccgc gcccacgagc
     1021 agggtatgag cgcggccatg cagtcgaacc gcctgagcgt gatgcgtatg gtcgggcgga
     1081 catggctgac catcacgatg gtgtcaggac tgctgttcgc cagcctgagc ggggtgttgt
     1141 ggtatcaggg gagcttgata gcgtcaaatc tggccgaaat cgaccggcag aacgcagcgc
     1201 tgtcgaagct gaacgcgaag acctggggcg tgacgtacct ggaagacagc aacggacgtt
     1261 ttctggtgct gccgaagggg acggccgtag acaggacgca gagctggacg gtcgggaacg
     1321 ggcggtcgaa gcagaacgcg ctgagactgg tgaaggagta agtgccgatg accgagctgg
     1381 aaaaacagtt gctgagcgca ttagagcagc tacagcagga ctactcgaaa aggctggacg
     1441 agtgggagag cgccttcggg gaatggcgga aaatgtcagg gcttatgcaa cgggagaacg
     1501 cggcgctgag cgagcgcgtg acgcgcttga gtcagcaggt ggaacgcttg agcgggcaac
     1561 tgcagcgttt gagccggtag tgcagcgtca tgagatggcc gtggccgcag aacgggcgca
     1621 cgagcaacgg cagcacgaaa aggaactgac ggaagcgcgt tcccggcagc gaagctatga
     1681 cggtccgtcg ctgggctgaa atgcaggaaa acagggttct catcgggcat ttacgctgaa
     1741 accggatgtc attcacctgg ctgcatggct atgcagccag gtaaaattta tctgtcgcga
     1801 acggggcggt ttatcgggtg gtttgttgcc ggttttacct gactgccacc ccgttcgcgg
     1861 cgaacccgtc cggggcggtg cgggcaacgg gtgttatgtt taatcagcgt gaacggcaga
     1921 tacaaaaaag ccccggaggg cttttaacta ctgacttatt tttttgaaga tttgctgggt
     1981 ttcaatttgt cagtatttcc cggcgttttt ttattgtcac gctggcgttt atcaggttcc
     2041 cctgttgaat cggcaattcg tttcggaccc ggcttaagac ccataatcgt tatcctttca
     2101 atagttaaga ggaaagccct caagctagat atattgaccg cgttacggat tatcaagagg
     2161 ggctaacaag attatttttt gttcttttag gttgattgtg gtactgttga tatacatgta
     2221 tagcaaatgg tgttcgggag gtttatatgc ttgctatccg gttatctgat gagattgagt
     2281 cccgtctgga ctcgctggcg aagcaaaccg gcagaacaaa gacgttttat gcgcgggaag
     2341 caatactggc gcatctggaa gacctggagg attattatct ttcagcagaa actgctgcac
     2401 gcgttcgccg tggtgatgaa gcagtgcatt cgtctgaaga cgtgaggaag tcacttggtc
     2461 tggacgatta actattccga tcgggcgctc aaatcgttac gcaagatgga caaacagaac
     2521 gcacgacgga ttgtggattt tatgagttta cgcattgcag ttgctgccga tcctcgccag
     2581 tcagggaagc cgctcaaagg tgagctgggc gagttctggc gctatcgggt gggagattat
     2641 cgcgttctgt gtgagatccg agatgacgag cttgttatcc ttgccgccac gattggacat
     2701 cgccgcgaag tttacgactg aagcccgcta tgcgggcttt tttgatgccg acaacaggta
     2761 ttatggtaaa tactcgacta actacgtgtt ctactgtttg tcggttttag ttaaccagca
     2821 ggttgtctta acgataatgt agccgttatt tttaactaat aaggtcatat tgttaacgtc
     2881 aggcataata aaagacatta tggcctcaga gttatcatca gaagcagctc tctgcattac
     2941 tacttcgatt cccttttctt tgaatctata agtatccagt tgcctattaa acttagaaag
     3001 acctacaacc atacttagtt tgttatcttt aacagtaaga gatgcttcct gagtaccatt
     3061 acatcggtag taagcggtgt aaccgtcagc aaaagatcga ccggggaata taaatgctag
     3121 gaacgcaatt aaccaataca tagttgagaa tcttggtatt acacatttca ctatcctctc
     3181 tttcattttt cgttcaccat actttgttat ctgattttta atttttctat tttataaacc
     3241 aagtctctac caaaaaggcg aaaattatcc tcaaataata ataattgttt aagaacatcc
     3301 atgaaagcag ctgcttcacc ctcagttatg ggagctactc tatataacat ataacctgag
     3361 ttaagggttt cttttatact ttcttcaatg ctcattataa atattagtgg attatcattt
     3421 ttcttccccc atgggtttct aattaatcct tcgatgtact caggtccttt gcgtcctatt
     3481 gcctgcgcaa tattttttat ttcatctgcg tgagggcttt gttgtacagc tttgtttatg
     3541 cctgcttgag tgattttcag agactctaca ccgtgtatgt tgtatgagat aaaatgtaat
     3601 aaattaagat gcaatgcaat taatccattt ttgtttacat ctgagtattt ggataaaaaa
     3661 cgtaatgaag tgttaactct cccttgaagc ctaacaattg ggagtgctgc cttgtatatt
     3721 tccttctccg ttaaataccg tttaaaaaac ataattctcc gcctccgtat agcgtgccta
     3781 ttttatttcg tcgtaaaact tagctctgaa atattccttt agaaccatca tgtcattaat
     3841 aattgaatct atctcatcca ttttttcttg gtattctttt aatggtgaag ggagcggtaa
     3901 ctcaatattt gaaaagtgat tggcgagata gggatcaagg tttctcatcc attcaggctt
     3961 ttcgccaatg cgttcttcaa ctgaaagtga acctgataat ctctggttaa taagatcact
     4021 tagactcatt ttggttttat tcgaaagccg ttgtaaggat tgctttaatc ctatatctag
     4081 catgatattt gttctttctc tcattacccc cccttgctat tgcgtttttt gttgcgcaca
     4141 aagctaccat tgcgcattaa gaaacgcaat aaaaaaggag ggagggagtg gtttgtacat
     4201 gatgtgcagt gcgctgaggg catcagtggc gggtgttttt ccgtgggatg gatggagctc
     4261 cggggataac agaactgacg atcatttaaa ggatcttctt gagatccttt ttttctgcgc
     4321 gtaatctctt gcactgtaaa cgaaaaaacc gcctggggag gcggtttgat cgaaggttaa
     4381 gtcagttggg gaactgctta accgggtaac tgggtatacg agaccgcaga taccaaaact
     4441 gttctttcag tgtagccgca gttaggcacc cacttcaaga cctctcaata tctctctcgt
     4501 atatatccag ttaccaatgg ctgctgccag tggcgttttt gcgtgtccgt ccgggttgga
     4561 ctcaagatga tagttaccgg aaagggcgca gcagtcgggc tgaacggggg gttcgtgcat
     4621 acagcccagc ttggagcgaa cgacctaaac cgagccgaga taccagcagc gtgagctatg
     4681 agaaagcgcc acgcttcccg aaagggaaaa aggcggaaca ggtatccggt aaacggcagg
     4741 gtcggaacag gagagcgcac gagggagccg ccggagagaa atcaccggca tctttaagtc
     4801 ctgtcgggtt tcgtcctcct ctgacttgag cgtccatttc tgtgatgctc gtcaggggag
     4861 gcggagccta tggaaaaacg gcaacgccgc atccttttct ccggcccttt ctgatttgag
     4921 cgcctttgct ttatcccgtt atccgtgagt cggtcacgcc cgccgcagcc gaacggcagg
     4981 agcgcagcta ccgagtgagc gaggaggcgt catttcatct gtccgtgcat ggtattgcgg
     5041 acgctcctga gccacatatc acacctgcca catgaggcac tttcatgaat cgtgctctca
     5101 tgccaacata gtaagccagt atacacagcg taaacgctgt gactggttca gggctgcgcc
     5161 ccgaaacccg ctaacaccac tgacgcgctg gcgcttgtcc aacgccggag cgacccggaa
     5221 aacttttccg tgccgtccgg cgtttgcgaa ggagggataa tgcagaagcc gaagaagaag
     5281 ccgctcgtcg taaaaaaaag atcctggcat ccggcagtga tggcaaatct gatgcgtcag
     5341 gcacaggagg aagattaccg ccgggagcag atggcagagc ttgagcgttc agcccggtca
     5401 cattttgcgg atatcggagg agaggatggc ggttaagcgt acccgttttt taggtatccg
     5461 ggtgaccgat ggggagtacc agcagctgct ggagcgctgc aatggaaggc aactggcggt
     5521 gtggatgcgg gaaacctgtc tcgatacgcg cccggcgcgg tcgttgcggc tgccgtccat
     5581 cgatccggtt ttgctg
//
