LOCUS       NZ_CP080159             8910 bp    DNA     circular CON 17-FEB-2024
DEFINITION  Escherichia coli strain PK8261 plasmid pPK8261-8kb, complete
            sequence.
ACCESSION   NZ_CP080159
VERSION     NZ_CP080159.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20353475
            Assembly: GCF_019428745.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8910)
  AUTHORS   Li,R., Lu,X., Munir,A., Abdullah,S., Liu,Y., Xiao,X., Wang,Z. and
            Mohsin,M.
  TITLE     Widespread prevalence and molecular epidemiology of tet(X4) and
            mcr-1 harboring Escherichia coli isolated from chickens in Pakistan
  JOURNAL   Sci Total Environ 806 (Pt 2), 150689 (2022)
   PUBMED   34599956
REFERENCE   2  (bases 1 to 8910)
  AUTHORS   Mohsin,M., Li,R. and Lu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUL-2021) Colloege of Veterinary Medicine, Yangzhou
            University, Shuangqiao Street, YangZhou, JiangSu 225000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2021
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/17/2024 08:12:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,557
            CDSs (total)                      :: 5,437
            Genes (coding)                    :: 5,135
            CDSs (with protein)               :: 5,135
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 302
            CDSs (without protein)            :: 302
            Pseudo Genes (ambiguous residues) :: 0 of 302
            Pseudo Genes (frameshifted)       :: 124 of 302
            Pseudo Genes (incomplete)         :: 205 of 302
            Pseudo Genes (internal stop)      :: 62 of 302
            Pseudo Genes (multiple problems)  :: 78 of 302
            Pseudo Genes (short protein)      :: 5 of 302
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080159.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="PK8261"
                     /isolation_source="chicken"
                     /db_xref="taxon:562"
                     /plasmid="pPK8261-8kb"
                     /geo_loc_name="Pakistan: Faisalabad"
                     /lat_lon="31.50 N 73.02 E"
                     /collection_date="2020-05-11"
                     /collected_by="Mashkoor Mohsin"
     gene            complement(1811..2044)
                     /locus_tag="KZY07_RS27435"
                     /old_locus_tag="KZY07_27440"
     CDS             complement(1811..2044)
                     /locus_tag="KZY07_RS27435"
                     /old_locus_tag="KZY07_27440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_029400396.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTEHLSQLYSESRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2051..2569)
                     /locus_tag="KZY07_RS27440"
                     /old_locus_tag="KZY07_27445"
     CDS             complement(2051..2569)
                     /locus_tag="KZY07_RS27440"
                     /old_locus_tag="KZY07_27445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_029400393.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKVLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMRQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2607..3305)
                     /locus_tag="KZY07_RS28265"
                     /old_locus_tag="KZY07_27450"
                     /pseudo=""
     CDS             complement(<2607..3305)
                     /locus_tag="KZY07_RS28265"
                     /old_locus_tag="KZY07_27450"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3247..3570)
                     /locus_tag="KZY07_RS27450"
                     /old_locus_tag="KZY07_27455"
     CDS             complement(3247..3570)
                     /locus_tag="KZY07_RS27450"
                     /old_locus_tag="KZY07_27455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3635..3826
                     /locus_tag="KZY07_RS27455"
                     /old_locus_tag="KZY07_27460"
     CDS             3635..3826
                     /locus_tag="KZY07_RS27455"
                     /old_locus_tag="KZY07_27460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165974.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4496..4654
                     /locus_tag="KZY07_RS27460"
                     /old_locus_tag="KZY07_27465"
     CDS             4496..4654
                     /locus_tag="KZY07_RS27460"
                     /old_locus_tag="KZY07_27465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001354639.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAAVGNLVSRAMMA
                     QMVQQSS"
                     /name="hypothetical protein"
     gene            5036..5377
                     /locus_tag="KZY07_RS27470"
                     /old_locus_tag="KZY07_27475"
     CDS             5036..5377
                     /locus_tag="KZY07_RS27470"
                     /old_locus_tag="KZY07_27475"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="WP_000058758.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNNEGYYFLASDKMLYAIVI
                     STILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 family microcin immunity protein"
     gene            complement(5374..6939)
                     /locus_tag="KZY07_RS27475"
                     /old_locus_tag="KZY07_27480"
     CDS             complement(5374..6939)
                     /locus_tag="KZY07_RS27475"
                     /old_locus_tag="KZY07_27480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_209200378.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETKRQLKLAEAEEKRLAALNEEARAVEVAQKNLAAAQSELAKVDEEIKTLNTRLSSSI
                     HARDAEMNTLSGKRNELAQASAKYKELDELVKKLSPRANDPLQSRPFFYATSRRTGAGK
                     IMEEKQKQVTASETRINQLNAEINGIQGAMSQANNNRNTAVLRVHESEENLKTAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALESVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKTVDAGVSYIVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTIND
                     VLGI"
                     /name="colicin-like pore-forming protein"
     gene            7217..7636
                     /locus_tag="KZY07_RS27480"
                     /old_locus_tag="KZY07_27485"
     CDS             7217..7636
                     /locus_tag="KZY07_RS27480"
                     /old_locus_tag="KZY07_27485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312320.1"
                     /translation="MAWHTLQNALKMTGRSRSQFYRDMRAGLVSYRTGQDGRREFETSE
                     LIRAYGELKQDETPERHSAGQAENPHDQQTERILRELNELRQCLTLMLEDKQAQATERR
                     RQEAEREQLHKEIEQLRQALEQEKKRGFWARLFSR"
                     /name="hypothetical protein"
     gene            7720..8139
                     /locus_tag="KZY07_RS27485"
                     /old_locus_tag="KZY07_27490"
     CDS             7720..8139
                     /locus_tag="KZY07_RS27485"
                     /old_locus_tag="KZY07_27490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254598.1"
                     /translation="MRRSGGTLYLIALLTAATVLTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            8665..>8841
                     /locus_tag="KZY07_RS27490"
                     /old_locus_tag="KZY07_27495"
                     /pseudo=""
     CDS             8665..>8841
                     /locus_tag="KZY07_RS27490"
                     /old_locus_tag="KZY07_27495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611469.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     Typing          complement(3912..3999)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.753"
                     /coverage="100.0"
                     /evalue="1.48e-35"
     Typing          4022..4568
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.841"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1708..3257)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.656"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaaaaaacc ctctactgaa gaggggtagt gtaaggaact tgctgatttt cttgatatta
       61 ttaaccatca ataactgagc atacaaaaaa taacaaaact ctgacacaga tacaaccatc
      121 cctctttgta taagaccgat gtgcatctca aaaaagaagc gtgtttctgg aacacagaac
      181 aacaatgcac attgtagcca acaaattcac accagaaaca cgctctgttt tttaatttac
      241 acaagaatat atgtctggaa aatcaggaag aagaaattaa tgggcattct gtatacgaag
      301 ggagagcgcg tttccggtgc cccatattag atattatccc tgcaacaaac accagagacg
      361 cgctcataat tcttctagcg taaatgatcc tttcaggcaa gaaaaaagcc gtgattctgt
      421 atcacggcct taattttatc gaaaacacgg attttagtat gcacatgact gtactcctgt
      481 ggagtacaag gaaatcataa tcagcgacca aaccatttat caacagaaaa ccaacatatt
      541 gatgttattt ttgtgacaaa tgttacaaat tcgaaaccac tgtattttta ctgcaacaaa
      601 atctggttga tctacaagtt acactggggt aaaaagttgt ccggaatgtt tctgcgtagt
      661 aaagcctcta gccggattgc ctttgcaaaa acgcaggaac gttcccccct tcatcagtac
      721 tcctgacgct gtaaggagct gactgatgca gacagggctg atgatatcta ataaaagcta
      781 tctcattttt gcgcgtacat aaaataaatc aggcacttcc ctacactccc cctccggaat
      841 tgagggggtt ttctttacaa tgtaaataaa ttgcttgcgt aaagttcaaa aaaactgcaa
      901 atttttactg tagtgtcgag cttcatgtag ctcaccaggg catggactaa aaagaagtaa
      961 acccatcttt taccccccct tatacggggg gttctttata gcacaaagaa cacttataat
     1021 ttatcatctc acagctataa atataaaaga ataatattct aaactacagt tatttatgca
     1081 gaacataaca tcaaactcac aataaatata aaacaattat tttataactg ttcacttatt
     1141 atattcagac atatcagaaa cacgactcaa ttctttttta tttaacaaaa tccataaaaa
     1201 acttatggtt gaaatcacat ttaaactgtg aaggatatca tccggagaaa tttttcaatc
     1261 cgacactcca tagaatggac ctttaagact ttatcctcat catgtatgag gatttttttt
     1321 ctggcaaaca gtatctttca gtaatccttg ctcagttccg attctgacgt atccccgtgt
     1381 ggatctgaaa aggagataat tacgctctgt ggccaacagg aataactcaa gcagctcctc
     1441 tctgatacca tcagaagaag cattaaacgc attctggcgt cgattacaga agaagcggaa
     1501 cgcgttccag gctcctgttg ctatacacac tacccacact aaccccatgg tttttgagcg
     1561 ccagaaacgc gtccgtatgc gttttaacgc gttcctgtgt tttcagcaag gcctgactga
     1621 agctagttcc tagtgccctg aaccaaccat tatccaaaaa aatgcaccgg aaacgcgctg
     1681 acaacacttt agcctgtctt tggttaccat cccagcgacg ggccatccag tgtccgttcc
     1741 tgtatttcca gatggcgctc acgctctagg aatagctcat gttcacgttc ctgtatcagt
     1801 agttcgtccc ttatctcctg ctctcgctgt ataactggct caagtgttct gtctgctcgc
     1861 tcaagtgctg cacctgctga ctcaactgca tgacccgctc gttcagcatc gcgttgtccc
     1921 gttgcgtaag cgaaaacatc ttctgcaatt ccacgaaggc gctctcccat tcgttcagcc
     1981 gctgcatata gtcctgttgc agctgctcta aggcgttcag caaatgtgtt tccagctctg
     2041 tcactctgtg tcactccttc agatgtaccc actctttccc ctgaaaggga atcacctctg
     2101 ctgatttccc gtacggaagg accagaaatt tcctgttccc gtcctgcacg aactccacgc
     2161 cccatgtctt cgcgttcagt ttctgcaatg tctcttcctg cttcctgata tcttccaggt
     2221 tcgcctgtat cctcccgcca agataccaga gcgtcccgcc actcgcggta aacaggagaa
     2281 agactatccc cagtaacatc atgcccgtat tccctgccag ctttaacacg tccttcctgt
     2341 gctgcctcat cgcctctttc accccttccc ggtgtttctc cagcgattcc tctgtcgagg
     2401 ctgtgaacag ggctatagcg tctctgattt tcgtctcgtt tgatgtcaca gcctcgctta
     2461 cagattcgcc gagcctcctg aactcgttgt tcagcacttt ctctgtagat tcggctctct
     2521 ctttcagctt tttctcgaac tccgcgcccg tctgtaaaag attgctcata aaacgctcct
     2581 ttcagcctga tattcttccc gccgttcggg tctgcaatgc tgatgctgct tcgcgtcacc
     2641 ctgaccactt caagccccgc ctctgtgagc gcctgaatca catcctgacg gccttttatc
     2701 tcccctacat gataaagagc gtctatcccg cgtgtgacgc tctcagcaag cgcctgtttc
     2761 gtttccggca ggttatcaga gagagtcagt atccggcggt tctccggggc gttggggtca
     2821 tgcagcccgt aatggtgatt caccagcgtc tgccaggcat caattctcgg cctgtctgcc
     2881 cggtcgtagt acggctggag acgttttccg gtctgtagct ccatgttcgg aatgacaaaa
     2941 ttcagctcaa gccgtccctt gtcctggtgc tccacccaca ggatgctgta ctgattcttt
     3001 tcgagacctg gcatcagtac gcgctcaaag ctcgccatca ctttttcacg tcctcccggc
     3061 ggcagctcct tctccgcgaa cgacagaacg cctgacgtgt atttcttcgc aaatggcgtg
     3121 gcatcgatga gttcccggac ttcttccgga ttaccctgaa gcaccgttgc gccttcgcgg
     3181 ttacgctccc tccccagcag gtaatcaacc ggaccactgc caccaccttt tcccctggca
     3241 tgaaatttaa ctatcatccc gcgccccctg ttccctgacg gccagacgca gccggcgcag
     3301 ctcatccccg atggccatca gtgcggccac cacctgaacc cgatcaccgg aagaccactg
     3361 cccgctgttc accttcctcg cggtctgatt caggttattc ccgatggcgg ccagctgacg
     3421 cagtaacggc ggtgccagtg tcggcagctt cccggaacgg gcaaccggct cccccaggca
     3481 gacccgccgc atccataccg ccagttgttt accctcacag cgttccagta accgggcatg
     3541 ttcatcatca gtaacccgta tcgtgagcat cctctcgcgc ttcatcggta tcattacccc
     3601 catgaacaga aatccccctt acacggaggc atcagtgact aaacaggaaa aaaccgccct
     3661 taacatggcc cgcttcatca gaggccagac attaacgctg ctggagaaac tcaacgaact
     3721 ggacgcggat gaacaggcag atatctgtga atcgcttcac gaccacgccg atgagcttta
     3781 ccgcagctgc ctcgcacgtt tcggggatga cggtgaaaac ctctgacaca tgcagctccc
     3841 ggagacggtc acagcttgtc tgtgagcgga tgccgggagc cgacaagccc gtcagggcgc
     3901 gtcagcaggt tttagcgggt gtcggggcgc agccctgacc cagtcacgta gcgatagcgg
     3961 agtgtatact ggcttagtca tgcggcatcg gtgcggattg tatgaaaagt gcaccatgta
     4021 cggtgtgaaa tgccgcacag atgcgtaagg agaacatgca gatgccgatg ctcttccgct
     4081 tcctcgctca ctgactcgct gcgctcggtc gttcggctgt ggcgagcggt gtctgctcac
     4141 tcaaaagcgg tgatactgtt atccacacaa tcaggggata acgccggaaa gaacatgtga
     4201 gcaaaaaacg aagaccccag aaaaggccgc gccggaggcg ctttttccat aggctccgcc
     4261 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4321 ttaaagatac caggcgtttc cccccggaag ctccctcgtg cgctctcctg ttccgaccct
     4381 gccgcttacc ggatacctct ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4441 ctcacgctgt tggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4501 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4561 cccggtaaga cacgacaaag cgccactggc agcagccgtt ggtaacttgg ttagcagagc
     4621 gatgatggcg caaatggtgc aacagagttc ttgaagtagt ggcccgacta cggctacact
     4681 ggaaggacag tatttggtat ctgcgctctg ctgaagccag ttacctcggt taagcagttc
     4741 cccaactgac ttaaccttcg atcaaaccgc ctccccaggc ggttttttcg tttacaggca
     4801 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctactgaaca
     4861 gagatccccg tcagtttaga agaagatgat ggagccacag tccctccctg aacatcacgt
     4921 atatagttag cctgacatcc aacaaggagg tttatagcta atattcccac aaaaaatctt
     4981 ttcctcataa ctcgatcctt ataaaatgaa aagaatatat aacgaggttt aatttatgag
     5041 tttaagatac tacataaaaa acattttgtt tggcctatat tgcacactta tatatatata
     5101 ccttataaca aaaaacaacg aagggtatta tttcctagcg tcagataaga tgctatacgc
     5161 aatcgtgata agcactattc tatgcccata ttcaaaatat gctattgaac acatattttt
     5221 taagttcata aagaaagatt ttttcagaaa aagaaaaaat ctaaataatg cccccgtggc
     5281 aaaattaaac ctatttatgc tatacaatct actttgtttg gtcctagcaa tcccatttgg
     5341 attgctagga ctgtttatat caataaagaa taattaaata cccaacacat catttatagt
     5401 attaagttta ttcttgtcaa taaaggcaca tagaatgcct gtaacaatag caatccccca
     5461 gatacctaat gtagttccag caagcacact aaaaagtaaa acaacaatat aactaactcc
     5521 agcatctaca gttttcttct ctaatgtaag aaatagtggc ttccagtcac ctgtatcctt
     5581 aatttttagg atatcagata ccacatcata tccaaaagaa acatgtcccg taatcttcaa
     5641 gtacttagca aactgatcta aatgcttagc ccagtcttca tacttaaccg attccagtgc
     5701 attgaaaatc gcatcacggt ctgctttgct gaatttctta tttaaaacat ccttgtattt
     5761 ttcaaaagca gctagagctt cattcacatt gctgattttc ttacctttag acttatcagc
     5821 aagttcctgt gccatttttg aatatttttc accatatttt tcagatagcg tttgataaaa
     5881 gctaactgtt gcatccacag catccttaat ctgcgagttc aggagattag tctgcgctgt
     5941 tttcaaattt tcttcagatt catgaacacg taaaacagct gtattacgat tattattagc
     6001 ctgagacata gccccctgaa ttccatttat ctcagcatta agttggttaa tacgcgtttc
     6061 tgatgctgtt acctgttttt gtttttcctc cataatctta ccggctcccg tccgtcgact
     6121 ggtggcatag aaaaaaggac gactctgaag cggatcatta gctcttggtg acaatttttt
     6181 gaccagttca tcaagttcct tatatttagc agatgcctga gccagctcat ttcgttttcc
     6241 ggacagcgta ttcatttctg catcacgggc atgaatactg gagcttaaac gggtattgag
     6301 agtcttaatc tcttcatcca ctttcgccag ctcagattgt gcagcagcaa gatttttttg
     6361 tgccacctcc actgcccggg cctcttcatt caatgctgcc agccgtttct cttcagcttc
     6421 agccagtttc aactggcgtt ttgtttcagc ctgctccttc tctatctctt tacgtcgttg
     6481 ttctgcctcc tgaaaagcct tttccgcagc ttccgcttct ttacgggctt tttcttctgc
     6541 ttttgcaagg cgcaaccgct ctgcttctgc ctgcatcgct gcattattgg catgagccag
     6601 gtcaataacc tccggatgag tggaattatg acgaagcgcc tcattcacaa tatccttcag
     6661 atgttgagtc agcgcatccc ggtttgcttt tgctttagcc tgtgcttctg cggcagcttt
     6721 tgcccgggca gcctgttctg cctgcgtttt cttcaattga gcagtagacc atttagctgt
     6781 ggcatgaatg gctgcagaac tttcactttt gctacctcca gttccaccac cgccgccaga
     6841 gccactcccg tctggattac cattcaaaag agtaatgatt acctgcccct tatcatcata
     6901 aggaacacca tctttatagt acgctacagc tgtttccatt ataaaatcct ctttgacatt
     6961 aaaaacaaat agttaaaagc aatatactgt atatataacc actggtttta tatacagctt
     7021 aaaattacgc cgctgcattt tccctgtcaa ccctgtggat tttcattttt gtgaaaacga
     7081 tcaaaaaaac agccacccac accacgtgct attcccaccc gatgccacaa aaaccagcac
     7141 aaacattacc gttctccggc cccagtatgt tttactgaaa ccatgatatg agacattaat
     7201 gggacactgt cacttcatgg catggcacac actccagaac gcactgaaaa tgacaggcag
     7261 atcgcgttca cagttttacc gtgatatgcg cgcaggcctt gtcagttatc gtacaggcca
     7321 ggacggacgg cgggagtttg aaaccagtga actgatccgg gcatacggcg aattaaagca
     7381 ggatgagaca ccagaaaggc acagtgcggg acaggcagaa aatccacatg atcagcagac
     7441 agaacgcatt ctccgggagc tgaatgagct gagacaatgc ctgacactga tgcttgagga
     7501 caaacaggca caggctacgg aacgccggcg ccaggaagcc gaacgggaac agctacataa
     7561 agagatagag cagctcaggc aggcactgga gcaggaaaaa aaacggggat tctgggccag
     7621 actgttcagc cggtgagcac cgactgcaag tgatgataag atagtcagcg gttaaatata
     7681 agtcactgag tacaaagatt taaaggtaag gagacactca tgagacgttc cggcggcaca
     7741 ctgtatctta tcgccctgct gacagcggca acggtcctga cagcctgcac gccaaagggc
     7801 agtgtggaac aacatacccg gcattacgtt tatgcatcag acgatggttt tgatcctaac
     7861 ttttccaccc agaaggccga cacaacacga atgatggtgc cttttttccg gcagttctgg
     7921 gatatgggag caaaggataa agcgacagga aaatcacgga gtgatgtgca gcaacgcatt
     7981 cagcagtttc acagccagga atttttaaac tcactccggg gcacaactca atttgcgggt
     8041 actgattacc gcagcaaaga ccttaccccg aaaaaatcca ggctgctggc tgagacgatt
     8101 tctgcggttt atctcgatgg ctacgaggga agacagtaag tggatttacc ataatccctt
     8161 aattgtacgc accgctgaaa tgcattcagc gcaatcacgg cagcagacag gtaaaaatcg
     8221 caacaacccc cccgaaaaac taccgcgatc gcgcctgata aattttaacc ttatgcatac
     8281 ctatgcatcc aggtgaatca caagcgaatt tccttactga gacgactgaa acggtttttt
     8341 ttcacaattt tcagctgttt tcgattccac aaaatagaag ttacaggata gggattaatc
     8401 cccttcgccg ccaaggggag caggatgagt caggggaatg tctattcacc tgcgctgtac
     8461 aggctgccag gtaaaagtat tcgctgcggc acgatactct tttgccctca tagcctttgc
     8521 gcttcttact tttcgttctt ccagaatcat tcttaagaca ttttcccgca cttttctcta
     8581 acttcccgtg gcaatttatc tctgccggac tgaaccgccc cggaaatcct ggagactaaa
     8641 cttcctgaga aagatgtaaa caggatgact aaaaatactc gtttttcccc cgaagtccgt
     8701 caacgggcag ttcgtatggt tctggaaagc cagggcgaat atgactcaca atgggcggca
     8761 atttgttcca ttgctccaaa gattggctgt acgccagaga ccctgcgtgt ctgggttcgc
     8821 cagcatgagc gggataccgg gtattacctt tcgaagaaat ttttgacatc ctctacgccc
     8881 tgaaggaccg ggttttacgg cgtaccagat
//
