LOCUS       NZ_CP080152             8909 bp    DNA     circular CON 17-FEB-2024
DEFINITION  Escherichia coli strain PK8276 plasmid pPK8276-8kb, complete
            sequence.
ACCESSION   NZ_CP080152
VERSION     NZ_CP080152.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20353404
            Assembly: GCF_019428725.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8909)
  AUTHORS   Li,R., Lu,X., Munir,A., Abdullah,S., Liu,Y., Xiao,X., Wang,Z. and
            Mohsin,M.
  TITLE     Widespread prevalence and molecular epidemiology of tet(X4) and
            mcr-1 harboring Escherichia coli isolated from chickens in Pakistan
  JOURNAL   Sci Total Environ 806 (Pt 2), 150689 (2022)
   PUBMED   34599956
REFERENCE   2  (bases 1 to 8909)
  AUTHORS   Mohsin,M., Li,R. and Lu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUL-2021) Colloege of Veterinary Medicine, Yangzhou
            University, Shuangqiao Street, YangZhou, JiangSu 225000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2021
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/17/2024 08:09:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,272
            CDSs (total)                      :: 5,154
            Genes (coding)                    :: 4,770
            CDSs (with protein)               :: 4,770
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 174 of 384
            Pseudo Genes (incomplete)         :: 232 of 384
            Pseudo Genes (internal stop)      :: 64 of 384
            Pseudo Genes (multiple problems)  :: 79 of 384
            Pseudo Genes (short protein)      :: 5 of 384
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080152.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="PK8276"
                     /isolation_source="chicken"
                     /db_xref="taxon:562"
                     /plasmid="pPK8276-8kb"
                     /geo_loc_name="Pakistan: Faisalabad"
                     /lat_lon="31.44 N 73.07 E"
                     /collection_date="2020-10-20"
                     /collected_by="Mashkoor Mohsin"
     gene            complement(join(8838..8909,1..315))
                     /locus_tag="KZY06_RS26080"
                     /old_locus_tag="KZY06_26065"
     CDS             complement(join(8838..8909,1..315))
                     /locus_tag="KZY06_RS26080"
                     /old_locus_tag="KZY06_26065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_085448100.1"
                     /translation="MTGRSRSQFYRDMRAGLVSYRTGQDGRREFETSELIRAYGELKQD
                     ETPERHSAGQAENPHDQQTERILRELNELRQCLTLMLEDKQAQATERRRQEAEREQLHK
                     EIEQLRQALEQEKKRGFWARLFSR"
                     /name="entry exclusion protein 1"
     gene            626..2191
                     /locus_tag="KZY06_RS26085"
                     /old_locus_tag="KZY06_26070"
     CDS             626..2191
                     /locus_tag="KZY06_RS26085"
                     /old_locus_tag="KZY06_26070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_000447001.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETERQLKLAEAEEKRLAALNEEARAVEVAQKNLAAAQSELAKVDEEIKTLNTRLSSSI
                     HARDAEMNTLSGKRNELAQASAKYKELDELVKKLSPRANDPLQSRPFFYATSRRTGAGK
                     IMEEKQKQVTASETRINQLNAEINGIQGAMSQANNNRNTAVLRVHESEENLKTAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALESVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKTVDAGVSYIVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTIND
                     VLGI"
                     /name="colicin-like pore-forming protein"
     gene            complement(2188..2529)
                     /locus_tag="KZY06_RS26090"
                     /old_locus_tag="KZY06_26075"
     CDS             complement(2188..2529)
                     /locus_tag="KZY06_RS26090"
                     /old_locus_tag="KZY06_26075"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="WP_000058758.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNNEGYYFLASDKMLYAIVI
                     STILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 family microcin immunity protein"
     gene            2577..2714
                     /locus_tag="KZY06_RS26095"
                     /old_locus_tag="KZY06_26080"
     CDS             2577..2714
                     /locus_tag="KZY06_RS26095"
                     /old_locus_tag="KZY06_26080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001519492.1"
                     /translation="MRKRFFVGILAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(2911..3069)
                     /locus_tag="KZY06_RS26100"
                     /old_locus_tag="KZY06_26085"
     CDS             complement(2911..3069)
                     /locus_tag="KZY06_RS26100"
                     /old_locus_tag="KZY06_26085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001354639.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAAVGNLVSRAMMA
                     QMVQQSS"
                     /name="hypothetical protein"
     gene            complement(3739..3930)
                     /locus_tag="KZY06_RS26105"
                     /old_locus_tag="KZY06_26090"
     CDS             complement(3739..3930)
                     /locus_tag="KZY06_RS26105"
                     /old_locus_tag="KZY06_26090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3994..4317
                     /locus_tag="KZY06_RS26110"
                     /old_locus_tag="KZY06_26095"
     CDS             3994..4317
                     /locus_tag="KZY06_RS26110"
                     /old_locus_tag="KZY06_26095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955996.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4526..>4957
                     /locus_tag="KZY06_RS26560"
                     /pseudo=""
     CDS             4526..>4957
                     /locus_tag="KZY06_RS26560"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4995..5513
                     /locus_tag="KZY06_RS26120"
                     /old_locus_tag="KZY06_26105"
     CDS             4995..5513
                     /locus_tag="KZY06_RS26120"
                     /old_locus_tag="KZY06_26105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_029400393.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKVLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMRQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            5520..5753
                     /locus_tag="KZY06_RS26125"
                     /old_locus_tag="KZY06_26110"
     CDS             5520..5753
                     /locus_tag="KZY06_RS26125"
                     /old_locus_tag="KZY06_26110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_029400396.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTEHLSQLYSESRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(<7633..7809)
                     /locus_tag="KZY06_RS26130"
                     /old_locus_tag="KZY06_26115"
                     /pseudo=""
     CDS             complement(<7633..7809)
                     /locus_tag="KZY06_RS26130"
                     /old_locus_tag="KZY06_26115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611469.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            complement(8335..8754)
                     /locus_tag="KZY06_RS26135"
                     /old_locus_tag="KZY06_26120"
     CDS             complement(8335..8754)
                     /locus_tag="KZY06_RS26135"
                     /old_locus_tag="KZY06_26120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254598.1"
                     /translation="MRRSGGTLYLIALLTAATVLTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     Typing          3573..3654
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          complement(2998..3544)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.841"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4308..5857
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.893"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctctttatgt agctgttccc gttcggcttc ctggcgccgg cgttccgtag cctgtgcctg
       61 tttgtcctca agcatcagtg tcaggcattg tctcagctca ttcagctccc ggagaatgcg
      121 ttctgtctgc tgatcatgtg gattttctgc ctgtcccgca ctgtgccttt ctggtgtctc
      181 atcctgcttt aattcgccgt atgcccggat cagttcactg gtttcaaact cccgccgtcc
      241 gtcctggcct gtacgataac tgacaaggcc tgcgcgcata tcacggtaaa actgtgaacg
      301 cgatctgcct gtcattttca gtgcgttctg gagtgtgtgc catgccatga agtgacagtg
      361 tcccattaat gtctcatatc atggtttcag taaaacatac tggggccgga gaacggtaat
      421 gtttgtgctg gtttttgtgg catcgggtgg gaatagcacg tggtgtgggt ggctgttttt
      481 ttgatcgttt tcacaaaaat gaaaatccac agggttgaca gggaaaatgc agcggcgtaa
      541 ttttaagctg tatataaaac cagtggttat atatacagta tattgctttt aactatttgt
      601 ttttaatgtc aaagaggatt ttataatgga aacagctgta gcgtactata aagatggtgt
      661 tccttatgat gataaggggc aggtaatcat tactcttttg aatggtaatc cagacgggag
      721 tggctctggc ggcggtggtg gaactggagg tagcaaaagt gaaagttctg cagccattca
      781 tgccacagct aaatggtcta ctgctcaatt gaagaaaacg caggcagaac aggctgcccg
      841 ggcaaaagct gccgcagaag cacaggctaa agcaaaagca aaccgggatg cgctgactca
      901 acatctgaag gatattgtga atgaggcgct tcgtcataat tccactcatc cggaggttat
      961 tgacctggct catgccaata atgcagcgat gcaggcagaa gcagagcggt tgcgccttgc
     1021 aaaagcagaa gaaaaagccc gtaaagaagc ggaagctgcg gaaaaggctt ttcaggaggc
     1081 agaacaacga cgtaaagaga tagagaagga gcaggctgaa acagaacgcc agttgaaact
     1141 ggctgaagct gaagagaaac ggctggcagc attgaatgaa gaggcccggg cagtggaggt
     1201 ggcacaaaaa aatcttgctg ctgcacaatc tgagctggcg aaagtggatg aagagattaa
     1261 gactctcaat acccgtttaa gctccagtat tcatgcccgt gatgcagaaa tgaatacgct
     1321 gtccggaaaa cgaaatgagc tggctcaggc atctgctaaa tataaggaac ttgatgaact
     1381 ggtcaaaaaa ttgtcaccaa gagctaatga tccgcttcag agtcgtcctt ttttctatgc
     1441 caccagtcga cggacgggag ccggtaagat tatggaggaa aaacaaaaac aggtaacagc
     1501 atcagaaacg cgtattaacc aacttaatgc tgagataaat ggaattcagg gggctatgtc
     1561 tcaggctaat aataatcgta atacagctgt tttacgtgtt catgaatctg aagaaaattt
     1621 gaaaacagcg cagactaatc tcctgaactc gcagattaag gatgctgtgg atgcaacagt
     1681 tagcttttat caaacgctat ctgaaaaata tggtgaaaaa tattcaaaaa tggcacagga
     1741 acttgctgat aagtctaaag gtaagaaaat cagcaatgtg aatgaagctc tagctgcttt
     1801 tgaaaaatac aaggatgttt taaataagaa attcagcaaa gcagaccgtg atgcgatttt
     1861 caatgcactg gaatcggtta agtatgaaga ctgggctaag catttagatc agtttgctaa
     1921 gtacttgaag attacgggac atgtttcttt tggatatgat gtggtatctg atatcctaaa
     1981 aattaaggat acaggtgact ggaagccact atttcttaca ttagagaaga aaactgtaga
     2041 tgctggagtt agttatattg ttgttttact ttttagtgtg cttgctggaa ctacattagg
     2101 tatctggggg attgctattg ttacaggcat tctatgtgcc tttattgaca agaataaact
     2161 taatactata aatgatgtgt tgggtattta attattcttt attgatataa acagtcctag
     2221 caatccaaat gggattgcta ggaccaaaca aagtagattg tatagcataa ataggtttaa
     2281 ttttgccacg ggggcattat ttagattttt tctttttctg aaaaaatctt tctttatgaa
     2341 cttaaaaaat atgtgttcaa tagcatattt tgaatatggg catagaatag tgcttatcac
     2401 gattgcgtat agcatcttat ctgacgctag gaaataatac ccttcgttgt tttttgttat
     2461 aaggtatata tatataagtg tgcaatatag gccaaacaaa atgtttttta tgtagtatct
     2521 taaactcata aattaaacct cgttatatat tcttttcatt ttataaggat cgagttatga
     2581 ggaaaagatt ttttgtggga atattagcta taaacctcct tgttggatgt caggctaact
     2641 atatacgtga tgttcaggga gggactgtgg ctccatcatc ttcttctaaa ctgacgggga
     2701 tctctgttca gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2761 ctgctgcctg taaacgaaaa aaccgcctgg ggaggcggtt tgatcgaagg ttaagtcagt
     2821 tggggaactg cttaaccgag gtaactggct tcagcagagc gcagatacca aatactgtcc
     2881 ttccagtgta gccgtagtcg ggccactact tcaagaactc tgttgcacca tttgcgccat
     2941 catcgctctg ctaaccaagt taccaacggc tgctgccagt ggcgctttgt cgtgtcttac
     3001 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3061 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat accaacagcg
     3121 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggagaggt atccggtaag
     3181 cggcagggtc ggaacaggag agcgcacgag ggagcttccg gggggaaacg cctggtatct
     3241 ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3301 ggggggcgga gcctatggaa aaagcgcctc cggcgcggcc ttttctgggg tcttcgtttt
     3361 ttgctcacat gttctttccg gcgttatccc ctgattgtgt ggataacagt atcaccgctt
     3421 ttgagtgagc agacaccgct cgccacagcc gaacgaccga gcgcagcgag tcagtgagcg
     3481 aggaagcgga agagcatcgg catctgcatg ttctccttac gcatctgtgc ggcatttcac
     3541 accgtacatg gtgcactttt catacaatcc gcaccgatgc cgcatgacta agccagtata
     3601 cactccgcta tcgctacgtg actgggtcag ggctgcgccc cgacaccccc taaaacctgc
     3661 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct cacagacaag ctgtgaccgt
     3721 ctccgggagc tgcatgtgtc agaggttttc accgtcatcc ccgaaacgtg cgaggcagct
     3781 gcggtaaagc tcatcggcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc
     3841 gtccagttcg ttgagctttt ccagcagcgt taatgtctgg cttctgataa agcgggccat
     3901 gttaagggct gttttttcct gtttagtcac tgatgcctcc gtgtaagggg gatttctgtt
     3961 catggggtaa tgataccgat gaaacgcgag aggatgctca cgatacgggt tactgatgat
     4021 gaacatgcca ggttacttga acgctgtgag ggtaaacaac tggcggtatg gatgcggcgg
     4081 gtatgcctgg gtgagccggt tgcccgttcc gggaagctgc cgacactggc accgccgtta
     4141 ctgcgtcagc tggctgccat cgggaataac ctgaatcaga cggcgaggaa ggtgaacagc
     4201 gggcagtggt cttccggtga ccgggttcag gtggtggccg cactgatggc catcgagaga
     4261 gagttgcgca gcttacggca ggtcgtcagg gaacagggag cgcgggatga tagttaaatt
     4321 tcatgccagg ggaaaaggcg gtggcagtgg tccggttgat tacctgctgg gaagggagcg
     4381 taaccgggaa ggggcaacgg tgcttcaggg taatccggaa gaagtccggg aactcatcga
     4441 tgccacgcca tttgcgaaga aatacacgtc aggtgttctg tcgttcgcgg agaaggagct
     4501 gccgccggga ggacgtgaaa aagtgatggc gagctttgag cgtgtactga tgcccggtct
     4561 cgaaaaaaat cagtacagca tcctgtgggt tgagcaccag gacaagggac ggcttgagct
     4621 gaattttgtc attccgaaca tggagctaca gagcggaaaa cgcctccagc cgtactatga
     4681 ccgcgcagac aggccgagaa ttgatgcctg gcagacgctg gtgaatcacc attacgggct
     4741 gcatgacccg aatgccccgg agaaccgcag gacgctgaca ctccctgata acctgccgga
     4801 aacgaaacag gcgattgctg agagcgttac acgggggctt gatgtgcttt accgtgccgg
     4861 agagataaaa gaccgtcagg atgtgattca ggcactcaca gatgtgggag ttgaggttgt
     4921 cagggtgacg cgaagcagta tcagcattgc agacccgaac ggcgggaaga atatcaggct
     4981 gaaaggagcg ttttatgagc aatcttttac agacgggcgc ggagttcgag aaaaagctga
     5041 aagagagagc cgaatctaca gagaaagtgc tgaacaacga gttcaggagg ctcggcgaat
     5101 ctgtaagcga ggctgtgaca tcaaacgaga cgaaaatcag agacgctata gccctgttca
     5161 cagcctcgac agaggaatcg ctggagaaac accgggaagg ggtgaaagag gcgatgaggc
     5221 agcacaggaa ggacgtgtta aagctggcag ggaatacggg catgatgtta ctggggatag
     5281 tctttctcct gtttaccgcg agtggcggga cgctctggta tcttggcggg aggatacagg
     5341 cgaacctgga agatatcagg aagcaggaag agacattgca gaaactgaac gcgaagacat
     5401 ggggcgtgga gttcgtgcag gacgggaaca ggaaatttct ggtccttccg tacgggaaat
     5461 cagcagaggt gattcccttt caggggaaag agtgggtaca tctgaaggag tgacacagag
     5521 tgacagagct ggaaacacat ttgctgaacg ccttagagca gctgcaacag gactatatgc
     5581 agcggctgaa cgaatgggag agcgccttcg tggaattgca gaagatgttt tcgcttacgc
     5641 aacgggacaa cgcgatgctg aacgagcggg tcatgcagtt gagtcagcag gtgcagcact
     5701 tgagcgagca gacagaacac ttgagccagt tatacagcga gagcaggaga taagggacga
     5761 actactgata caggaacgtg aacatgagct attcctagag cgtgagcgcc atctggaaat
     5821 acaggaacgg acactggatg gcccgtcgct gggatggtaa ccaaagacag gctaaagtgt
     5881 tgtcagcgcg tttccggtgc atttttttgg ataatggttg gttcagggca ctaggaacta
     5941 gcttcagtca ggccttgctg aaaacacagg aacgcgttaa aacgcatacg gacgcgtttc
     6001 tggcgctcaa aaaccatggg gttagtgtgg gtagtgtgta tagcaacagg agcctggaac
     6061 gcgttccgct tcttctgtaa tcgacgccag aatgcgttta atgcttcttc tgatggtatc
     6121 agagaggagc tgcttgagtt attcctgttg gccacagagc gtaattatct ccttttcaga
     6181 tccacacggg gatacgtcag aatcggaact gagcaaggat tactgaaaga tactgtttgc
     6241 cagaaaaaaa atcctcatac atgatgagga taaagtctta aaggtccatt ctatggagtg
     6301 tcggattgaa aaatttctcc ggatgatatc cttcacagtt taaatgtgat ttcaaccata
     6361 agttttttat ggattttgtt aaataaaaaa gaattgagtc gtgtttctga tatgtctgaa
     6421 tataataagt gaacagttat aaaataattg ttttatattt attgtgagtt tgatgttatg
     6481 ttctgcataa ataactgtag tttagaatat tattctttta tatttatagc tgtgagatga
     6541 taaattataa gtgttctttg tgctataaag aaccccccgt ataagggggg gtaaaagatg
     6601 ggtttacttc tttttagtcc atgccctggt gagctacatg aagctcgaca ctacagtaaa
     6661 aatttgcagt ttttttgaac tttacgcaag caatttattt acattgtaaa gaaaaccccc
     6721 tcaattccgg agggggagtg tagggaagtg cctgatttat tttatgtacg cgcaaaaatg
     6781 agatagcttt tattagatat catcagccct gtctgcatca gtcagctcct tacagcgtca
     6841 ggagtactga tgaagggggg aacgttcctg cgtttttgca aaggcaatcc ggctagaggc
     6901 tttactacgc agaaacattc cggacaactt tttaccccag tgtaacttgt agatcaacca
     6961 gattttgttg cagtaaaaat acagtggttt cgaatttgta acatttgtca caaaaataac
     7021 atcaatatgt tggttttctg ttgataaatg gtttggtcgc tgattatgat ttccttgtac
     7081 tccacaggag tacagtcatg tgcatactaa aatccgtgtt ttcgataaaa ttaaggccgt
     7141 gatacagaat cacggctttt ttcttgcctg aaaggatcat ttacgctaga agaattatga
     7201 gcgcgtctct ggtgtttgtt gcagggataa tatctaatat ggggcaccgg aaacgcgctc
     7261 tcccttcgta tacagaatgc ccattaattt cttcttcctg attttccaga catatattct
     7321 tgtgtaaatt aaaaaacaga gcgtgtttct ggtgtgaatt tgttggctac aatgtgcatt
     7381 gttgttctgt gttccagaaa cacgcttctt ttttgagatg cacatcggtc ttatacaaag
     7441 agggatggtt gtatctgtgt cagagttttg ttattttttg tatgctcagt tattgatggt
     7501 taataatatc aagaaaatca gcaagttcct tacactaccc ctcttcagta gaggggtttt
     7561 tttatctggt acgccgtaaa acccggtcct tcagggcgta gaggatgtca aaaatttctt
     7621 cgaaaggtaa tacccggtat cccgctcatg ctggcgaacc cagacacgca gggtctctgg
     7681 cgtacagcca atctttggag caatggaaca aattgccgcc cattgtgagt catattcgcc
     7741 ctggctttcc agaaccatac gaactgcccg ttgacggact tcgggggaaa aacgagtatt
     7801 tttagtcatc ctgtttacat ctttctcagg aagtttagtc tccaggattt ccggggcggt
     7861 tcagtccggc agagataaat tgccacggga agttagagaa aagtgcggga aaatgtctta
     7921 agaatgattc tggaagaacg aaaagtaaga agcgcaaagg ctatgagggc aaaagagtat
     7981 cgtgccgcag cgaatacttt tacctggcag cctgtacagc gcaggtgaat agacattccc
     8041 ctgactcatc ctgctcccct tggcggcgaa ggggattaat ccctatcctg taacttctat
     8101 tttgtggaat cgaaaacagc tgaaaattgt gaaaaaaaac cgtttcagtc gtctcagtaa
     8161 ggaaattcgc ttgtgattca cctggatgca taggtatgca taaggttaaa atttatcagg
     8221 cgcgatcgcg gtagtttttc gggggggttg ttgcgatttt tacctgtctg ctgccgtgat
     8281 tgcgctgaat gcatttcagc ggtgcgtaca attaagggat tatggtaaat ccacttactg
     8341 tcttccctcg tagccatcga gataaaccgc agaaatcgtc tcagccagca gcctggattt
     8401 tttcggggta aggtctttgc tgcggtaatc agtacccgca aattgagttg tgccccggag
     8461 tgagtttaaa aattcctggc tgtgaaactg ctgaatgcgt tgctgcacat cactccgtga
     8521 ttttcctgtc gctttatcct ttgctcccat atcccagaac tgccggaaaa aaggcaccat
     8581 cattcgtgtt gtgtcggcct tctgggtgga aaagttagga tcaaaaccat cgtctgatgc
     8641 ataaacgtaa tgccgggtat gttgttccac actgcccttt ggcgtgcagg ctgtcaggac
     8701 cgttgccgct gtcagcaggg cgataagata cagtgtgccg ccggaacgtc tcatgagtgt
     8761 ctccttacct ttaaatcttt gtactcagtg acttatattt aaccgctgac tatcttatca
     8821 tcacttgcag tcggtgctca ccggctgaac agtctggccc agaatccccg ttttttttcc
     8881 tgctccagtg cctgcctgag ctgctctat
//
