LOCUS       NZ_CP080147            12396 bp    DNA     circular CON 17-FEB-2024
DEFINITION  Escherichia coli strain PK8276 plasmid pPK8276-tetX, complete
            sequence.
ACCESSION   NZ_CP080147
VERSION     NZ_CP080147.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20353404
            Assembly: GCF_019428725.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12396)
  AUTHORS   Li,R., Lu,X., Munir,A., Abdullah,S., Liu,Y., Xiao,X., Wang,Z. and
            Mohsin,M.
  TITLE     Widespread prevalence and molecular epidemiology of tet(X4) and
            mcr-1 harboring Escherichia coli isolated from chickens in Pakistan
  JOURNAL   Sci Total Environ 806 (Pt 2), 150689 (2022)
   PUBMED   34599956
REFERENCE   2  (bases 1 to 12396)
  AUTHORS   Mohsin,M., Li,R. and Lu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUL-2021) Colloege of Veterinary Medicine, Yangzhou
            University, Shuangqiao Street, YangZhou, JiangSu 225000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2021
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/17/2024 08:09:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,272
            CDSs (total)                      :: 5,154
            Genes (coding)                    :: 4,770
            CDSs (with protein)               :: 4,770
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 384
            CDSs (without protein)            :: 384
            Pseudo Genes (ambiguous residues) :: 0 of 384
            Pseudo Genes (frameshifted)       :: 174 of 384
            Pseudo Genes (incomplete)         :: 232 of 384
            Pseudo Genes (internal stop)      :: 64 of 384
            Pseudo Genes (multiple problems)  :: 79 of 384
            Pseudo Genes (short protein)      :: 5 of 384
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080147.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="PK8276"
                     /isolation_source="chicken"
                     /db_xref="taxon:562"
                     /plasmid="pPK8276-tetX"
                     /geo_loc_name="Pakistan: Faisalabad"
                     /lat_lon="31.44 N 73.07 E"
                     /collection_date="2020-10-20"
                     /collected_by="Mashkoor Mohsin"
     gene            1..840
                     /locus_tag="KZY06_RS23790"
                     /old_locus_tag="KZY06_23780"
     CDS             1..840
                     /locus_tag="KZY06_RS23790"
                     /old_locus_tag="KZY06_23780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_219808276.1"
                     /translation="MATHKPINILEAFTAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVLYLPAEDPPTAIHHRLHALGAYLNDEQRQAVAA
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLEKQRKSKGVRRGEA"
                     /name="helicase RepA family protein"
     gene            827..1678
                     /gene="repC"
                     /locus_tag="KZY06_RS23795"
                     /old_locus_tag="KZY06_23785"
     CDS             827..1678
                     /gene="repC"
                     /locus_tag="KZY06_RS23795"
                     /old_locus_tag="KZY06_23785"
                     /function="putative replication protein"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_010895881.1"
                     /translation="MVKPKNKYSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFKGPEPLGADDLRILQGLVAMAGPNGLVLSPEPKTEGGQQLRLFLEPKWEAV
                     TADAMVVKGSYRALAREIGYAEDGGSQFKAIRECIERLWTVSIIAQNGRKRQGFRLLAE
                     YASDEAGGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSEAARLLHQRLCGWIDPGK
                     TGKAAIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            <1727..2182
                     /locus_tag="KZY06_RS23800"
                     /old_locus_tag="KZY06_23790"
                     /pseudo=""
     CDS             <1727..2182
                     /locus_tag="KZY06_RS23800"
                     /old_locus_tag="KZY06_23790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            2965..3810
                     /gene="estT"
                     /locus_tag="KZY06_RS23805"
                     /old_locus_tag="KZY06_23795"
     CDS             2965..3810
                     /gene="estT"
                     /locus_tag="KZY06_RS23805"
                     /old_locus_tag="KZY06_23795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014053561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide hydrolase EstT"
                     /protein_id="WP_164538741.1"
                     /translation="MKEKIIKTNGIELCTESFGNKKNPAILLVAGATVSMLYWDTEFCQ
                     QLSEKGFFVIRYDNRDVGKSTNYEPGSAPYDIVDLTNDAIAILDGYEIDKAHFVGMSLG
                     GLITQIAAIKFADRVSSLTLMSTGPWGDSDPTIPEMDTRILDFHGKAGTVDWSNEDSVV
                     NYLIQGAELMCGKKQFDKQRSEKLIRAEFNRANNYISMFNHATLQGSEEYWNRLNEVNQ
                     PTLIIHGTDDKIWHYKNAGVLIEKIQNTKLITLEGTGHELHFEDWTTIIDGIEKHINEQ
                     "
                     /name="estT"
     gene            3800..4957
                     /locus_tag="KZY06_RS23810"
                     /old_locus_tag="KZY06_23800"
     CDS             3800..4957
                     /locus_tag="KZY06_RS23810"
                     /old_locus_tag="KZY06_23800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014615010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline-inactivating monooxygenase Tet(X4)"
                     /protein_id="WP_094309310.1"
                     /translation="MSNKEKQMNLLSDKNVAIIGGGPVGLTMAKLLQQNGIDVSVYERD
                     NDREARIFGGTLDLHKGSGQEAMKKAGLLQTYYDLALPMGVNIADEKGNILSTKNVKPE
                     NRFDNPEINRNDLRAILLNSLENDTVIWDRKLVMLEPGKKKWTLTFENKPSETADLVII
                     ANGGMSKVRKFVTDTEVEETGTFNIQADIHHPEVNCPGFFQLCNGNRLMAAHQGNLLFA
                     NPNNNGALHFGISFKTPDEWKNQTQVDFQNRNSVVDFLLKEFSDWDERYKELIRVTSSF
                     VGLATRIFPLGKSWKSKRPLPITMIGDAAHLMPPFAGQGVNSGLMDALILSDNLTNGKF
                     NSIEEAIENYEQQMFIYGKEAQEESTQNEIEMFKPDFTFQQLLNV"
                     /name="tetracycline-inactivating monooxygenase Tet(X4)"
     gene            5296..>6522
                     /locus_tag="KZY06_RS23815"
                     /old_locus_tag="KZY06_23805"
                     /pseudo=""
     CDS             5296..>6522
                     /locus_tag="KZY06_RS23815"
                     /old_locus_tag="KZY06_23805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /name="IS91-like element ISVsa3 family transposase"
     gene            <6504..6905
                     /locus_tag="KZY06_RS23820"
                     /old_locus_tag="KZY06_23810"
                     /pseudo=""
     CDS             <6504..6905
                     /locus_tag="KZY06_RS23820"
                     /old_locus_tag="KZY06_23810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747810.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
                     /name="DUF3363 domain-containing protein"
     gene            7123..8337
                     /gene="floR"
                     /locus_tag="KZY06_RS23825"
                     /old_locus_tag="KZY06_23815"
     ARG             7124..8334
                     /name="floR"
                     /gene="floR"
                     /gene_name="chloramphenicol/florfenicol efflux MFS
                     transporter FloR"
                     /tool="amrfinderplus"
                     /drug_class="PHENICOL"
                     /identity="99.5"
                     /coverage="100.0"
     gene            8365..>8484
                     /locus_tag="KZY06_RS26465"
                     /pseudo=""
     CDS             8365..>8484
                     /locus_tag="KZY06_RS26465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /name="LysR family transcriptional regulator"
     gene            complement(8439..8789)
                     /locus_tag="KZY06_RS23830"
                     /old_locus_tag="KZY06_23820"
     CDS             complement(8439..8789)
                     /locus_tag="KZY06_RS23830"
                     /old_locus_tag="KZY06_23820"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_223424540.1"
                     /translation="MAFAIMRAKKLNSMGTVAAALQHCYRDRETPNADQERTPDNDHLA
                     ARSTDEAMGKLRERLPEKRRKDAVLAVEYVMSASPEWWQTASADQQREFFKRSTEWLAA
                     CRKFRCSATAMN"
                     /name="plasmid recombination protein"
     gene            complement(9299..9466)
                     /locus_tag="KZY06_RS23835"
                     /old_locus_tag="KZY06_23825"
     CDS             complement(9299..9466)
                     /locus_tag="KZY06_RS23835"
                     /old_locus_tag="KZY06_23825"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_161403145.1"
                     /translation="MTDLKLDDYTDADFIDELLSDELRCECGEELHYYDDGSLSRCNSC
                     DPDRETNPNL"
                     /name="hypothetical protein"
     gene            complement(9493..9777)
                     /locus_tag="KZY06_RS23840"
                     /old_locus_tag="KZY06_23830"
     CDS             complement(9493..9777)
                     /locus_tag="KZY06_RS23840"
                     /old_locus_tag="KZY06_23830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_094336938.1"
                     /translation="MAKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPA"
                     /name="mobilization protein"
     gene            9977..>10618
                     /locus_tag="KZY06_RS26470"
                     /pseudo=""
     CDS             9977..>10618
                     /locus_tag="KZY06_RS26470"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            10725..11138
                     /locus_tag="KZY06_RS26475"
     CDS             10725..11138
                     /locus_tag="KZY06_RS26475"
                     /inference="COORDINATES: protein motif:HMM:NF036929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_223384840.1"
                     /translation="MSAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRETQAAWAKLAKELREVASEVSDAAQSARSAARGWHWKLWLTVMAASM
                     MPTLVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            11237..12106
                     /locus_tag="KZY06_RS26480"
     CDS             11237..12106
                     /locus_tag="KZY06_RS26480"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_229025478.1"
                     /translation="MNREWSASEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDL
                     SELDLEDMKAEGREPALIVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASA
                     DSRHYGRLAGFTNRKDKHTTRTGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQ
                     EKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRYGDDLSKCDFIAAQKLASRGRS
                     AEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELAQAPAPRQRGMD
                     RGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            12162..12368
                     /gene="urfF"
                     /locus_tag="KZY06_RS23850"
                     /old_locus_tag="KZY06_23840"
     CDS             12162..12368
                     /gene="urfF"
                     /locus_tag="KZY06_RS23850"
                     /old_locus_tag="KZY06_23840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_094336940.1"
                     /translation="MKDHRDTQTGNLLATPNAIRQARFADAQRKLGRKARKIWATDDEA
                     EALRLYLEELRAAQGGGSDPASA"
                     /name="urfF"
     ARG             3801..4954
                     /name="tet(X4)"
                     /gene="tet(X4)"
                     /gene_name="tetracycline-inactivating monooxygenase
                     Tet(X4)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          9732..9888
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.671"
                     /coverage="100.0"
                     /evalue="1.08e-59"
     Typing          19..840
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.205"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          9978..12103
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.769"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctaccc ataagcctat caatattctg gaggcgttca cagcagcgcc gccaccgctg
       61 gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt
      121 gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg
      181 ctggaggtgg gcgaactgcc caccggcccg gtgctctacc tgcccgccga agatccaccc
      241 accgccatcc atcaccggct tcacgccctg ggcgcatacc tcaacgacga gcagcggcaa
      301 gccgtggccg ctggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc
      361 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac
      421 acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc
      481 ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc
      541 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg
      601 gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc
      661 gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag
      721 gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc
      781 aaacccgccg tgctggaaaa gcagcgcaaa agcaaggggg tgcgccgtgg tgaagcctaa
      841 gaacaagtac agtctcagcc acgtccggca cgacccggcg cactgtctgg ctcccggcct
      901 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
      961 cggcgacggc aagcggatcg agttcaaggg gccggagccg ctgggtgctg atgatctgcg
     1021 catcctgcaa gggctggtgg caatggctgg gcctaatggc ctagtgctta gcccagaacc
     1081 caagaccgaa ggcgggcagc agctccggtt gttcttggaa cccaagtggg aggccgttac
     1141 cgctgatgcc atggtggtta agggcagcta tcgggcgctg gcccgtgaaa tcgggtatgc
     1201 agaggatggc ggcagccagt tcaaggcaat ccgggagtgc attgagcgcc tgtggacagt
     1261 ctccatcatc gcccaaaatg gccgcaagcg gcaggggttc cggctgcttg ccgagtacgc
     1321 cagcgacgag gcaggcgggc gtctgtacgt ggccctgaac cccttaattg ctcaggccgt
     1381 catgggtggt ggccagcatg tgcgcatcag catggacgag gtgcgagcgc tggacagcga
     1441 agcggcccgc ctgctgcatc agcgcctgtg tggctggatc gaccccggca agaccggcaa
     1501 ggcggccata gataccctgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
     1561 gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag ctggttgcgc tgggctggac
     1621 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
     1681 ccctgtttaa cagtcacgcc cctccccctc cgcactgtca gattgctacc agctcaagac
     1741 gccgtaccgg gacggcacca cgcacgtcat tttcgaacca ttggatttca ttgcaaggct
     1801 ggccgccctg gtaccgaagc ccagagtcaa cctaacccgc ttccacgggg tgttcgcacc
     1861 caacagtcgg caccgggcgt tggtcacgcc ggcaaaacgg ggcaggggca acaaggtcag
     1921 ggtggctgat gaaccggcaa caccagcaca acggcgagcg tcgatgacat gggcgcaacg
     1981 gctcaagcgt gttttcaata tcgacatcga gacctgcagc ggctgcggcg gcgccatgaa
     2041 agtcatcgcc tgcattgaag accctatagt gatcaagcag atccttgatc acctgaagca
     2101 caaagccgaa accagcggga ccagggcgtt acccgaaagc cgggcgccac cggctgagct
     2161 gctcctgggt ctgtttgact gacgagcctg aaggccaacg ataccaatca aaatgctgcg
     2221 ttcacagcgc cgcggcaggg atccgccgtg ctggttgtcg gaaaaggagc cgctagtggg
     2281 aaagaggagg gtaaattttc agcgttgctg gctccccgtc agccggattg ggttgcatcg
     2341 caggggtgtc gaaagagtca actgcggtcc aaagctgttg gacttgggtg aaaagggtgt
     2401 ttattcttcc tatacgtcaa taggaaatat tgatgaaatt tgttgcttaa gattgtttgc
     2461 tggtgtctgt aaatcaaggt tctgagcaat agaactctgt gcgatagcaa aggtcgctat
     2521 cgacatataa attaatttct ttttcatgga ttttcttttt caaaacccaa gttataaatt
     2581 ttttactaac tataaactcc tttttctgtt aattttaaat tttaacattt gacacaactt
     2641 taatatattt atatatacat atatttttat atattatatg atttcataag atttgaaaca
     2701 taaataatta agcactataa gtactaaaat gttaaaataa tttttatatt tgagaaattc
     2761 aaaatgcgaa ataaaatagt aaaaacattc gcctatcttg ccatatgggt atgaaataga
     2821 gtagaatgtt tcgtatatat ataagttagc gggcattgtg aaagatacca gcataacagt
     2881 taaacattga taaatgaaaa aaaactactt tggatattaa ttttaggact gataataatc
     2941 agttgcaaac aaaggaaaac agaaatgaaa gagaaaataa ttaaaacaaa cggcattgaa
     3001 ctctgtacgg aaagttttgg aaataagaaa aatccagcaa tccttttggt agcaggtgca
     3061 accgtatcaa tgctgtattg ggacactgaa ttttgccaac aattatctga aaaaggattt
     3121 tttgttattc gttacgacaa cagagatgta ggaaaatcca ctaattatga gccgggttca
     3181 gcaccatacg acattgttga cttaacaaat gatgccattg caattttgga cggctatgaa
     3241 atagacaaag cacattttgt gggtatgtca ttaggtgggc taattacaca aatagcagct
     3301 ataaagtttg ccgatagagt tagctcatta acacttatgt ctacaggccc ttggggagac
     3361 tcggacccaa ctattcctga gatggacaca cgtattttag atttccatgg taaagcaggc
     3421 acagtcgatt ggtcaaatga agacagtgtg gtaaactatt taattcaggg tgcagaatta
     3481 atgtgcggca agaaacaatt tgataaacaa agaagtgaaa agctgataag agctgagttc
     3541 aatagagcaa acaattatat aagtatgttc aatcacgcta cattgcaagg tagtgaagaa
     3601 tattggaata gactaaatga agtaaatcaa ccaaccttga taattcacgg aacagatgac
     3661 aaaatttggc actataaaaa cgctggtgta ttaattgaga aaatacaaaa tacgaaatta
     3721 attactcttg aaggaacagg acacgaattg cactttgaag attggacaac aataattgac
     3781 ggaattgaaa aacatataaa tgagcaataa agaaaaacaa atgaatttac ttagtgataa
     3841 gaacgttgca ataattggtg gtggacccgt tggactgact atggcaaaat tattacagca
     3901 aaacggcata gacgtttcag tttacgaaag agacaacgac cgagaggcaa gaatttttgg
     3961 tggaaccctt gacctacaca aaggttcagg tcaggaagca atgaaaaaag cgggattgtt
     4021 acaaacttat tatgacttag ccttaccaat gggtgtaaat attgctgatg aaaaaggcaa
     4081 tattttatcc acaaaaaatg taaagcccga aaatcgattt gacaatcctg aaataaacag
     4141 aaatgactta agggctatct tgttgaatag tttagaaaac gacacggtta tttgggatag
     4201 aaaacttgtt atgcttgaac ctggtaagaa gaagtggaca ctaacttttg agaataaacc
     4261 gagtgaaaca gcagatctgg ttattattgc caatggtgga atgtctaaag taagaaaatt
     4321 tgttaccgac acggaagttg aagaaacagg tactttcaat atacaagccg atattcatca
     4381 tccagaggtg aactgtcctg gattttttca gctatgcaat ggaaaccggc taatggctgc
     4441 tcatcaaggt aatttattat ttgcgaatcc taataataat ggtgcattgc attttggaat
     4501 aagttttaaa acacctgatg aatggaaaaa ccaaacgcag gtagattttc aaaacagaaa
     4561 tagtgtcgtt gattttcttc tgaaagaatt ttccgattgg gacgaacgct acaaagaact
     4621 gattcgtgtg acatcatctt ttgtagggtt agcgacacga atatttccct taggtaagtc
     4681 ttggaaaagt aagcgtccat tacccataac gatgattgga gatgctgctc atttgatgcc
     4741 tccttttgca ggacaaggcg taaacagcgg gttgatggat gccttgatat tgtcggataa
     4801 tctgaccaat gggaaattta acagcattga agaggctatt gaaaattatg aacagcaaat
     4861 gtttatctat ggcaaagaag cacaagaaga atcaactcaa aacgaaattg aaatgtttaa
     4921 acccgacttt acgtttcagc aattgttaaa tgtataaaat ggaataaaac ggcacataat
     4981 cgagtagacg gctccgccag taactgacgg agtgcgcctc tcacaccacc gagcgtacgg
     5041 gtcacgtact cggcggttcg caaagagatg ggttaagttg taaatgtaat tcagttaagg
     5101 aaacatatcc tcttttcttc aggcgttgta aagtaatggt agtgcctaga ataggacttt
     5161 gtgccaacta ttgagtggga gtgacgggca ctggctggca atgtctagca acggcaggca
     5221 tttcggctga gggtaaaaga actttccgct aagcgataga ctgtatgtaa acacagtatt
     5281 gcaaggacgc ggaacatgcc tcatgtggcg gccaggacgg ccagccggga tcgggatact
     5341 ggtcgttacc agagccaccg acccgagcaa acccttctct atcagatcgt tgacgagtat
     5401 tacccggcat tcgctgcgct tatggcagag cagggaaagg aattgccggg ctatgtgcaa
     5461 cgggaatttg aagaatttct ccaatgcggg cggctggagc atggctttct acgggttcgc
     5521 tgcgagtctt gccacgccga gcacctggtc gctttcagct gtaagcgtcg cggtttctgc
     5581 ccgagctgtg gggcgcggcg gatggccgaa agtgccgcct tgctggttga tgaagtactg
     5641 cctgaacaac ccatgcgtca gtgggtgttg agcttcccgt ttcagctgcg tttcctgttt
     5701 gccagccggc ccgagatcat ggggtgggtg ctgggcatcg tttaccgcgt cattgccacg
     5761 cacctggtca agaaagcggg ccatacccac caagtggcca agacgggcgc ggtcaccctg
     5821 atccagcgtt ttggatcggc gctcaatctg aatgttcact tccacatgct gtttctcgac
     5881 ggtgtgtatg tcgagcaatc ccacggctca gcgcgtttcc gctgggtcaa ggcgccgacc
     5941 agcccagagc tcacccagct gacgcacacc atcgcccacc gggtgggtcg ctatctggaa
     6001 cggcaaggcc tgctggaacg ggatgtcgaa aacagctatc tggcctcgga tgcggtggat
     6061 gacgacccga tgacacccct gctggggcac tcgatcactt accgtatcgc tgtcggttca
     6121 caggcggggc gaaaggtgtt cactttgcaa actctgccga ccagtggtga tccgttcggt
     6181 gacgggattg gcaaggtagc cgggtccagc ctgcacgccg gcgtggcggc cagggccgat
     6241 gaacgcaaga agctcgaacg gctgtgccgg tacatcagcc gcccggcggt atccgagaag
     6301 cggctgtcgt taacacgagg cggcaacgtg cgctaccagc tcaagacgcc gtaccgggac
     6361 ggcaccacgc acgtcatttt cgaaccattg gatttcattg caaggctggc cgccctggta
     6421 ccgaagccca gagtcaacct aacccgcttc cacggggtgt tcgcacccaa cagtcggcac
     6481 cgggcgttgg tcacgccggc aaaacggggc aggggcaaca agtccgcgaa gccatggacc
     6541 agcgccgcga gcaccatatc gaacagggcg acgccacccg cagccgggac agccgcgtct
     6601 tctaccggcg caaccttctc gccatcctgc gggagcgcga ggtagccggc gtcggatcgg
     6661 atatggcttt gagtaagggc ctgccgttcc gcgccgccac ggacggcgag agcgtcagcg
     6721 gcaagtttac cggaaccgtg catctatcga gcggcaagtt cgccgtggtc gagaaatccc
     6781 atgagttcac ccttgtcccg tggcggccga tcatcgaccg ccaactcggc cgcgaggtta
     6841 tgggcatcgt gcagggcggg tcggtgtcgt ggcagttagg gcggcagagg gggctggaac
     6901 gctgagtgcg cccatgccgc attgcgaatg caaaagataa tcggataaaa tgtagcaatt
     6961 catattcgta agcgtggagt aatcagatgg gaaattccaa gtcagcagac aagtaagccg
     7021 caacaaccag tattgttgtt gcggcgctct gtaaggctag tctcatctga ttgctgacga
     7081 gcagacgtcg cccggtattc cttaatcgag ggttgattcg tcatgaccac cacacgcccc
     7141 gcgtgggcct atacgctgcc ggcagcactg ctgctgatgg ctcctttcga catcctcgct
     7201 tcactggcga tggatattta tctccctgtc gttccagcga tgcccggcat cctgaacacg
     7261 acgcccgcta tgatccaact cacgttgagc ctctatatgg tgatgctcgg cgtgggccag
     7321 gtgatttttg gtccgctctc agacagaatc gggcgacggc caattctact tgcgggcgca
     7381 acggctttcg tcattgcgtc tctgggagca gcttggtctt caactgcacc ggcctttgtc
     7441 gctttccgtc tacttcaagc agtgggcgcg tcggccatgc tggtggcgac gttcgcgacg
     7501 gttcgcgacg tttatgccaa ccgtcctgag ggtgtcgtca tctacggcct tttcagttcg
     7561 atgctggcgt tcgtgcctgc gctcggccct atcgccggag cattgatcgg cgagttcttg
     7621 ggatggcagg cgatattcat tactttggct atactggcga tgctcgcact cctaaatgcg
     7681 ggtttcaggt ggcacgaaac ccgccctctg gatcaagtca agacgcgccg atctgtcttg
     7741 ccgatcttcg cgagtccggc tttttgggtt tacactgtcg gctttagcgc cggtatgggc
     7801 acctacttcg tcttcttctc gacggctccc ggtgtgctca taggccaagc ggaatattcc
     7861 gagatcggat tcagctttgc cttcgccact gtcgcgcttg taatgatcgt gacaacccgt
     7921 ttcgcgaagt cctttgtcgc cagatggggc atcgcaggat gcgtggcgcg tgggatggcg
     7981 ttgcttgttt gcggagcggt cctgttgggg atcggcgaac tttacggctc gccgtcattc
     8041 ctcaccttca tcctaccgat gtgggttgtc gcggtcggta ttgtcttcac ggtgtccgtt
     8101 accgcgaacg gcgctttggc agagttcgac gacatcgcgg gatcagcggt cgcgttctac
     8161 ttctgcgttc aaagcctgat agtcagcatt gtcgggacat tggcggtggc acttttaaac
     8221 ggtgacacag cgtggcccgt gatctgttac gccacggcga tggcggtact ggtttcgttg
     8281 gggctggtgc tccttcggct ccgtggggct gccaccgaga agtcgccagt cgtctaaccg
     8341 acgactggta gcaggcccgc tccgatgcgg cgcactaacc atcgaaacct cgtgaatgtc
     8401 ggtatcctgt ctggcaggat accgctcatt tcccttgttc agttcatcgc cgtcgccgag
     8461 catctgaatt ttcggcatgc ggccagccac tcggtcgatc gcttgaagaa ctcccgctgc
     8521 tggtcggcgc ttgcggtctg ccaccactcc ggcgaggcgc tcatcacgta ttcgaccgcc
     8581 agcaccgcat ccttgcgccg cttctccggc aaccgctccc tgagcttgcc catcgcctca
     8641 tcggtcgatc tggccgccag gtgatcgttg tccggtgtgc gctcctgatc ggcgttcggc
     8701 gtctcgcggt cgcggtagca gtgctgcaac gcagccgcca ccgtgcccat gctgttgagc
     8761 ttcttcgccc tcatgatcgc aaacgccatc cgtcccccgg cgcaactgtc acgcccccgg
     8821 cgtaactgtc acgccccccc ggcgtaactg tcacgccccc ccggcgtaac tgtcacgaac
     8881 ccccggcgta actgtcacgc ccctaaacct gcaaacccag gaaggggggg agctggtggg
     8941 gtgttggaaa aatccatcca tgattatcca agaataatcc actaggcgcg gttaacagcg
     9001 ccctagtggg gcgctgtttg cccttgctta aaatgccggc cagaggccgg atagctggtc
     9061 tattcgctgc gctaggctac acaccgcccc accgctgcgc ggcaggggga aaggcgggca
     9121 aagcccgcta aaccccacac caaaccccgc agaaatacgc tggagcgctt ttagccgctt
     9181 tagcggcctt tccccctacc cgaagggtgg gggcgcgtgt gcagccccgc agggcctgtc
     9241 tcggtcgatc attcagcatc ggcatcatct gctaatttcc cgcaatgttc gcaggtaatc
     9301 ataggtttgg attggtttct ctatctggat cacacgaatt gcagcgactc aatgaaccat
     9361 catcgtaata atgaagctcc tctccgcatt cacagcgcag ttcatcactg agcagttcat
     9421 caataaaatc agcgtctgtg taatcgtcta gttttaaatc agtcattatt gctttccttt
     9481 attagtttaa attcaggccg gctcatcctt ctggcgtggc ggcagaccga acaaggcccg
     9541 gtcgtggtcg cgttcaaggt acgcatccat tgccgccatg agccgatctt ctggccactc
     9601 gctgctgttc accttggcca gaatcatggc gcccaccagc accttgcgcc gcgtttcgtt
     9661 cttgcgctct tgctgctgct cccttgccct cacccgctga atctcggcat tgatccgtgc
     9721 tcgctgttct tcgagcttgg ccagcctatc cgccgccttg ttgctcccct tagccatctt
     9781 gacacctcat tgttaatgtg ctgtctctta ggctatcatg gaggcacgac ggcggcaaac
     9841 cccgaccgta ctttgtacgg gagggcgcac ttaccggttt ctcttcgaga aactggccta
     9901 acggccaccc ttcgggcggt gcgctctccg agggccattg catggagccg aaaagcaaaa
     9961 gcaacagcga ggcagcatgg cgatttatca ccttacggcg aaaaccggca gcaggtcggg
    10021 cggccaatcg gccaaggcca aggccgacta catccagcgc gaaggcaagt atgcccgcga
    10081 catggatgaa gtgctgcacg ccgaatccgg gcacatgccg gagttcgtcg agcggcccgc
    10141 cgactactgg gatgctgccg acctgcatga acgcgccaat gggcggctgt tcaaggaggt
    10201 cgaatttgcc ctgccggtcg agctgaccct cgaccagcag aaggcgctgg cgtccgagtt
    10261 cgcccagcac ctgaccggtg ccgaacgttt gccgtatacg ctggccatcc atgccggtgg
    10321 cggcgagaac ccgcactgcc acctgatgat ctccgagcgg atcaatgacg gcatcgagcg
    10381 gcccgccgct cagtggttca agcggtacaa cggcaaggcc ccggagaagg gcggagcgca
    10441 gaagaccgaa gcactcaagc ccaaggcatg gcttgagcag acccgcgagg catgggccga
    10501 ccatgccaac cgggcattag agcgggctgg ccacgacgcc cgcattgacc accgaacgct
    10561 tgaggcgcag ggcatcgagc gcctgcccgg tgttcacctg gggccgaacg tggtggagat
    10621 ggaaggccgg ggcattcgca ccgaccgggc agacgtggcc ctgaacatcg acaccgccaa
    10681 cgcccagatc atcgacttgc aggaataccg ggaggctata gaccatgagc gcaatcgaca
    10741 gagtgaagaa atccagagga atcaacgagt tagcggagca gatcgagccg ctggcccaga
    10801 gcatggcgac actggccgac gaggcccggc aggtcatgag ccagacccag caggccagcg
    10861 aggcgcaggc agcggagtgg ctgaaagccc agcgcgagac acaggcagca tgggccaagc
    10921 tggccaagga attgcgggaa gtggccagcg aggtgagcga cgccgcgcag agcgcccgga
    10981 gcgcggccag agggtggcac tggaagctgt ggctaaccgt gatggcggct tccatgatgc
    11041 ctacgctggt gctgctgatc gcatcgttgc tcttgctcga cctgacgcca ctgacaacag
    11101 aggacggctc gatctggctg cgcttggtgg cccgatgaaa aacgacagaa ccttgcaggc
    11161 cataggccga cagctcaagg ccatgggctg tgagcgcttc gatatcggcg tcagggatgc
    11221 caccaccggc cagatgatga accgggaatg gtcagcctcc gaagtgctcc agaacacgcc
    11281 atggctcaag cggatgaatg cccagggcaa tgacgtgtat atcaggcccg ccgagcagga
    11341 acgccatggt ctggtgctgg tggacgacct cagcgagctc gacctggagg acatgaaagc
    11401 cgagggccgg gaaccggcgc tgatcgtgga gaccagcccg aagaactatc aggcatgggt
    11461 caaggtggcc gacgccgcag gcggtgaact ccgggggcag attgcccgga cgctggccag
    11521 cgagtacgac gccgacccgg ccagcgccga cagccgccac tatggccgct tggcgggttt
    11581 caccaaccgc aaggacaagc acaccacccg caccggctat cagccgtggg tgctgctgcg
    11641 tgagtccaag ggcaagaccg ccaccgctgg cccggcgctg gtgcagcagg ctggccagca
    11701 gatcgagcag gcccagcggc agcaggagaa ggcccgcagg ctggccagcc tcgaactgcc
    11761 cgagcggcag cttagccgcc accggcgcac ggcgctggac gagtaccgca gcgagatggc
    11821 cggactggtc aagcgctacg gtgacgacct cagcaagtgc gactttatcg ccgcgcagaa
    11881 actggccagc cggggccgca gtgccgagga aatcggcaag gccatggccg aggccagccc
    11941 ggcgctggca gagcgcaagc ccggccacga agcggattac atcgagcgca ccgtcagcaa
    12001 ggttatgggt ctgcccagcg tccagcttgc gcgggccgag ctggcacagg caccggcacc
    12061 ccgccagcga ggcatggaca ggggcgggcc agatttcagc atgtaacgct tgcattagcg
    12121 ctaacgggta gtatactgtt agctctaatg gaggatttga gatgaaagac catcgggaca
    12181 cgcagaccgg caacctgttg gccacgccca acgcgatcag gcaggctagg tttgccgacg
    12241 ctcagcgaaa actgggccgc aaggcccgta agatatgggc cacagacgac gaggccgagg
    12301 cgctgcgtct gtacctggaa gaactcagag cggcgcaggg cgggggtagt gaccccgcca
    12361 gcgcctaacc accaactgcc tgaacaggag gcaatc
//
