LOCUS       NZ_CP080138             6680 bp    DNA     circular CON 17-FEB-2024
DEFINITION  Escherichia coli strain PK8277 plasmid pPK8277-6kb, complete
            sequence.
ACCESSION   NZ_CP080138
VERSION     NZ_CP080138.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20349091
            Assembly: GCF_019428665.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   Li,R., Lu,X., Munir,A., Abdullah,S., Liu,Y., Xiao,X., Wang,Z. and
            Mohsin,M.
  TITLE     Widespread prevalence and molecular epidemiology of tet(X4) and
            mcr-1 harboring Escherichia coli isolated from chickens in Pakistan
  JOURNAL   Sci Total Environ 806 (Pt 2), 150689 (2022)
   PUBMED   34599956
REFERENCE   2  (bases 1 to 6680)
  AUTHORS   Mohsin,M., Li,R. and Lu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUL-2021) Colloege of Veterinary Medicine, Yangzhou
            University, Shuangqiao Street, YangZhou, JiangSu 225000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2021
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/17/2024 08:01:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,870
            CDSs (total)                      :: 4,752
            Genes (coding)                    :: 4,475
            CDSs (with protein)               :: 4,475
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 277
            CDSs (without protein)            :: 277
            Pseudo Genes (ambiguous residues) :: 0 of 277
            Pseudo Genes (frameshifted)       :: 84 of 277
            Pseudo Genes (incomplete)         :: 190 of 277
            Pseudo Genes (internal stop)      :: 50 of 277
            Pseudo Genes (multiple problems)  :: 45 of 277
            Pseudo Genes (short protein)      :: 3 of 277
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080138.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="PK8277"
                     /isolation_source="chicken"
                     /db_xref="taxon:562"
                     /plasmid="pPK8277-6kb"
                     /geo_loc_name="Pakistan: Faisalabad"
                     /lat_lon="31.44 N 73.07 E"
                     /collection_date="2020-10-20"
                     /collected_by="Mashkoor Mohsin"
     gene            complement(join(6251..6680,1..83))
                     /locus_tag="KZY04_RS24405"
     CDS             complement(join(6251..6680,1..83))
                     /locus_tag="KZY04_RS24405"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_232379250.1"
                     /translation="MENALRKKLDANAGYSGLNCKNPNHDHWKIAVWQRELYTLDWLAD
                     SLDLNTEYDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYDQWLQACYERANAYNL
                     QFAVPLEESEVRGITKSIAKLTHMKMTERFFEEYIKKTHSQEIQRKRECLGGLKSKGGG
                     RPSLGSP"
                     /name="replication initiation protein"
     gene            complement(29..412)
                     /locus_tag="KZY04_RS24410"
     CDS             complement(29..412)
                     /locus_tag="KZY04_RS24410"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_232379251.1"
                     /translation="MRNAALELFNDRLPHKPYFSDDLHFGVRMAGKERAILAKYIQFNQ
                     PHPMFWLGFDVDRVGAAIDWSDRNAPALTITNPDNGHAHLLYALETSIRTAPNGKMKPL
                     KYAAAQWRTRCVKNWMLMRGIRD"
                     /name="replication initiation protein"
     gene            complement(944..1312)
                     /locus_tag="KZY04_RS24040"
                     /old_locus_tag="KZY04_24065"
     CDS             complement(944..1312)
                     /locus_tag="KZY04_RS24040"
                     /old_locus_tag="KZY04_24065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_121863227.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIHREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLADADSVSAAETNFSEASGPDNGFFLSGNDFMRAEEE
                     SQRVVESFRDSLSRKKGF"
                     /name="hypothetical protein"
     gene            complement(1944..2175)
                     /locus_tag="KZY04_RS24415"
                     /pseudo=""
     CDS             complement(1944..2175)
                     /locus_tag="KZY04_RS24415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332449.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            2405..2536
                     /locus_tag="KZY04_RS24650"
                     /old_locus_tag="KZY04_24070"
     CDS             2405..2536
                     /locus_tag="KZY04_RS24650"
                     /old_locus_tag="KZY04_24070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_074442714.1"
                     /translation="MHEPPVQSDRCVLSGNYRLESNPERHAKTPLAAAIGKQDVREM"
                     /name="replication initiation protein"
     gene            2919..3559
                     /locus_tag="KZY04_RS24050"
                     /old_locus_tag="KZY04_24075"
     CDS             join(2919..3168,3168..3559)
                     /locus_tag="KZY04_RS24050"
                     /old_locus_tag="KZY04_24075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558270.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_232379019.1"
                     /translation="MASISISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGYRASARIMGVGLNTILRHFKKLRPQSVNSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYVYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWLLYESRLKGELHVISKRYTQRIERHNLKLRQHLARLGRKSLSFSK"
                     /name="IS1 family transposase"
     gene            3870..5032
                     /locus_tag="KZY04_RS24055"
                     /old_locus_tag="KZY04_24080"
     CDS             join(3870..4131,4131..5032)
                     /locus_tag="KZY04_RS24055"
                     /old_locus_tag="KZY04_24080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="WP_085947598.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYESQ
                     LYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKYVF
                     IEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSKQRY
                     GAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLLEQDF
                     YASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDALQMALW
                     RRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVEC
                     IHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENQNLA"
                     /name="IS3-like element IS3 family transposase"
     gene            5081..6229
                     /locus_tag="KZY04_RS24060"
                     /old_locus_tag="KZY04_24085"
     CDS             5081..6229
                     /locus_tag="KZY04_RS24060"
                     /old_locus_tag="KZY04_24085"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="WP_121863229.1"
                     /translation="MYILAALLSLIVSFFILRGLNYQKGIYNSNGIESISGLRALLALA
                     VLLSHSTHYLYSLEKKWIYDANYKEFFGIGNIYLNLGKVGVLMFFMISGFLFYRLVYKE
                     SINTRMFLKKRILRIVPAYWFSMIVIIIIGCFYYKVDYNISSLIELFRWAFFIGDYSIG
                     GISTADINAGVDWTLKIEWILYLSLPVLFFMTRKKSKMHKDISILISILIIFTIAFMIR
                     LYAGIYTDPRPVLGFASGLLAFRFSEKTKKFKNNKLLGYVAASFIVLSLTLGTYAVFYL
                     SMVLLCTFSFLIVSSGNTIGNILSNKTIVSLGEASYSIYLIHGIVLFLMKKVDMASKIN
                     TFLEIFLFNLILIIITAYASKLLHLKIERRWFNKDLSIPKES"
                     /name="acyltransferase"
     Typing          2031..2514
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.776"
                     /coverage="93.0"
                     /evalue="7.660000000000002e-166"
ORIGIN
        1 cagtggtcgt ggtttgggtt tttacagttc agtcccgaat accccgcatt agcatccagt
       61 tttttacgca gcgcgttctc cactgcgcag cagcgtactt cagcggcttc atttttccgt
      121 tcggcgcggt gcggatcgat gtttccaggg cgtatagcag gtgcgcgtgt ccgttgtcag
      181 gattggtgat ggtcagcgcg ggcgcgttcc ggtcgctcca gtcgatagca gcgccgacac
      241 gatccacgtc aaatcccagc cagaacatgg ggtgaggctg attgaactga atgtatttcg
      301 ccaggatagc gcgctctttc cccgccatgc gtacaccgaa atgcaaatcg tccgagaagt
      361 acggcttgtg cggtaagcgg tcgttaaaaa gttcgagtgc ggcgttacgc aatgtgtcct
      421 gaacttatgg agggctgttg tcttgcagcg tgcatatgct agtatccttc ttaggaattt
      481 agtcctgcgc cctcgtcatc gtgatcttcc gccaagttga taacacgatt tcgggggcgt
      541 ttgctttatg gggttaaaac tacacccagt tcaaccagtt gtcgacttta cctcgattta
      601 ccataacatc cgttgcgtgc accgcctgag aggctctgag ggcgtttctg acggaaaacc
      661 ggtaaaacag gcaacaaacc acccgaaaac ccgccagaaa cgcgcaggct ctgtttttac
      721 ggcatgcatg agtatgcgcg caagtgcata gcgcagaatt tcacgccgtg cagccagtgg
      781 aagtcctgtt ttcgacgttc ttctcatccg aaaacggtga acggggacgc aatttattgc
      841 gtggccgtga ggcattgacc aagccataag gcacggtagg taattgcagg tatccgacaa
      901 tttcgagtag aggatccact atttcactac ttagaattca aaatcagaat cctttcttcc
      961 tgctaaggct atctctgaat gattcaacca ctctttgaga ttcttcttct gctcgcatga
     1021 agtcattccc ggacagaaaa aaaccattgt caggcccact ggcttcagaa aaattcgttt
     1081 ctgcagcaga cacagaatcc gcatcagcaa gagacggttc atctgtactt tttctcaatc
     1141 tgtaaagagc cttatcaaac gcaaccagtg agatattaag gccgtaacgt tcattcagca
     1201 cagtcacaat ctcttcccta tggatgccat ttttcttcca ggtttctata tcgttcttgg
     1261 aatccctgac agccgcaatg gctgttcttg gaccagggtt aaaattcgcc atataccgcc
     1321 cataatgtct taaatttgtc tcataatagt ccaaaaataa gagtgaaacg caaaaaatgt
     1381 cttacaattg tcttattgtt gtccttcccg gttcatgtat ccatgaaccg caggatatgg
     1441 aacgcttttt atcgatctca tgggctgcca aagatagatt cattctttaa aagtcaggtt
     1501 acattatccc ttaaatggcc atacacctaa cgcagccgcg attttcatcg caactgcatg
     1561 aattaaatta aaaaaatgga gttctttcgt gtattagcgt aatacgcaac gtattacaat
     1621 catctgtaac aacatgaaaa taaacaaata acatgtaata cgctggtatt acatgtacgc
     1681 tggccagcga aaaagctata acgattcccg gccgacaggc ggcccggaat ccacaggggc
     1741 atgcaggttc tcttcagtaa ccttcgccat taaatcaaaa aggtcatatt cggcaggaag
     1801 ctgcgtatca gtagaatccc gtagttcatc atattcagat ggcatggggt gatatccagt
     1861 agctgagagg ttgttagcgg atttcggggc gtagccctga accagtcaca tagcactagc
     1921 ggagtgtaga cgggcttaat attttaaacc gaagtggtga tttgccaaag tgcttcatgt
     1981 ggcaggagtg aaatgaggct ttccggcgtc aggagaagat gctggttcag gtgatattcc
     2041 gcttcctcgc tcactgactc gctacgctcg gtcgttcggc tgcggcgagc ggacgaagct
     2101 cacggataag agcataaagc tatgacacag gaataaagag gtgtccgaaa ggatgcggcg
     2161 ttgccgtttt tccataggct ccgcccccct gacgagtatc aaaaaatctg acgctcaaat
     2221 cagtggcggc gaaacccgac aggactataa agatcccagg cgtttccccc tggtagctcc
     2281 ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg
     2341 cgtttgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctgga
     2401 ctgtatgcac gaaccccccg ttcagtccga ccgctgcgtc ttgtccggta actatcgtct
     2461 tgagtccaat ccggaaagac acgcaaaaac gccactggca gcggccattg gtaaacagga
     2521 tgtgcgagag atgtagtaag agttcttgaa gtgatggcct gagtgcggct acactgaaag
     2581 gacagatttg gtgacatgtc tcgcacaaga ctgttaccac ggttagaaaa tctgccagga
     2641 ttaactaacc ttcgaaaaac cacctgccag ggtggttttt tcgtttacag agcaagagat
     2701 tacgacgcaa cagaaaggaa ctcaagaagt tccttgctgt attgctacta tcctagagat
     2761 ctgacccatt aggggcatta tcagattacg gccttgtaat ccacgataaa aacccagaaa
     2821 ccagaagagt tggcccagcg acatatgtta cataaacgcc cacggtaatg gtgccaactt
     2881 actgatttag tgtacgatgg tgtttttgag gagctcccgt ggcttccatc tccatcagtt
     2941 gtccctcctg ctcagctact gacggggtgg tgcgtaacgg taaaagtact gccggacatc
     3001 agcgctatct ctgctctcac tgccgtaaaa catggcagct acagttcact tacaccgcct
     3061 ctcagcccgg tacgcaccag aaaatcattg atatggccat gaatggcgtc ggatatcgcg
     3121 ccagtgcacg cattatgggc gttggcctca acacgatttt acgacactta aaaaactcag
     3181 gccgcagtcg gtaaactcac gcatacaacc gggcagtgac gtcattgttt gcgcggaaat
     3241 ggacgaacag tggggttacg tcggcgctaa atcacgccag cgctggttgt tttacgtgta
     3301 tgacaggata cggaggacgg ttgtggcgca cgtattcggt gaacgcacgc tggccacgct
     3361 ggagcgtctt ctgagcctgc tgtcggcctt tgaggtcgtg gtatggatga cggatggctg
     3421 gctgctttat gaatcacgcc tgaagggaga actgcacgtt atcagcaagc gatatacgca
     3481 gcgcattgag cgacataacc tgaagctgag acaacatctg gcaagactgg gacggaagtc
     3541 actgtcgttc tcaaaatagg tggagctgca tgacaaggtc atcgggcatt atctgaacat
     3601 aaaacactat cagtaagttg gagtcattac ccactctcac aaaggagaag aacaaaggaa
     3661 acaagagaga tgagtggtaa gtgcaagata aatcgggagt taattagatt aattagggga
     3721 ggaagccctc tggcaaaaga gggctgttat ctgacacagg cttgtttagt ctcattttag
     3781 taatgataat gcgttaccga aaattgatct tacccagcaa tagtggacac gcggctaagt
     3841 gagtaaactc tcagtcagag gtgactcaca tgacaaaaac agtatcaacc agtaaaaaac
     3901 cccgtaaaca gcattcgcct gaatttcgca gtgaagccct gaagcttgct gaacgcatcg
     3961 gtgttactgc cgcagcccgt gaactcagcc tgtatgaatc acagctctac aactggcgca
     4021 gtaaacagca aaatcagcag acgtcttctg aacgtgaact ggagatgtct accgagattg
     4081 cacgtctcaa acgccagctg gcagaacggg atgaagagct ggctatcctc caaaaggccg
     4141 cgacatactt cgcgaagcgc ctgaaatgaa gtatgtcttt attgaaaaac atcaggctga
     4201 gttcagcatc aaagcaatgt gccgcgtgct ccgggtggcc cgcagcggct ggtatacgtg
     4261 gtgtcagcgg cggacaagga taagcacgcg tcagcagttc cgccaacact gcgacagcgt
     4321 tgtcctcgcg gcttttaccc ggtcaaaaca gcgttacggt gccccacgcc tgacggatga
     4381 actgcgtgct cagggttacc cctttaacgt aaaaaccgtg gcggcaagcc tgcgccgtca
     4441 gggactgagg gcaaaggcct cccggaagtt cagcccggtc agctaccgcg cacacggcct
     4501 gcctgtgtca gaaaatctgt tggagcagga tttttacgcc agtggcccga accagaagtg
     4561 ggcaggagac atcacgtact tacgtacaga tgaaggctgg ctgtatctgg cagtggtcat
     4621 tgacctgtgg tcacgtgccg ttattggctg gtcaatgtcg ccacgcatga cggcgcaact
     4681 ggcctgcgat gccctgcaga tggcgctgtg gcggcgtaag aggccccgga acgttatcgt
     4741 tcacacggac cgtggaggcc agtactgttc agcagattat caggcgcaac tgaagcggca
     4801 taatctgcgt ggaagtatga gcgcaaaagg ttgctgctac gataatgcct gcgtggaaag
     4861 cttctttcat tcgctgaaag tggaatgtat ccatggagaa cactttatca gccgggaaat
     4921 aatgcgggca acggtgttta attatatcga atgtgattac aatcggtggc ggcggcacag
     4981 ttggtgtggc ggcctcagtc cggaacaatt tgaaaaccag aacctcgctt aggcctgtgt
     5041 ccatattacg tgggtaggat caaatgacag gtaacgcata atgtatatac ttgcagcact
     5101 tttatctctg atcgtgagct tttttatctt gcgaggatta aactatcaaa aaggaattta
     5161 taatagtaat gggatagaaa gtattagcgg attaagagcc ctccttgctc ttgctgtgct
     5221 tttatctcac tcaactcatt atttgtactc tcttgaaaaa aagtggatct atgatgctaa
     5281 ttacaaagag ttttttggta tcgggaatat atatttaaac ttaggaaagg ttggtgtttt
     5341 gatgttcttt atgatatcag ggttcctttt ctatagatta gtatataaag aatctataaa
     5401 tacgaggatg tttttgaaaa aacgaattct tagaatagtt ccggcttatt ggttcagtat
     5461 gatagtaata ataataattg gctgctttta ttacaaggtg gactataaca tatcatctct
     5521 aatcgagctt tttagatggg cgttttttat aggggactat tctattggag ggatcagtac
     5581 tgcggatata aacgcaggcg ttgactggac tttaaaaata gaatggattt tatacttatc
     5641 actcccagtt ttatttttca tgacaagaaa aaagagtaaa atgcataagg atataagtat
     5701 attgataagc atactaatta tattcacaat agcttttatg attagacttt acgcggggat
     5761 ttacacagat ccgaggcctg ttctagggtt tgcatctggg ttgcttgcat ttagattctc
     5821 ggaaaaaacg aagaaattta agaataataa gctattaggt tatgtggcag cgtcttttat
     5881 tgttttatct ttaactttgg gaacatatgc agttttttat ttatccatgg tactactttg
     5941 tactttttca tttttaatag tgtcgtctgg aaatacaata ggaaacatac tatcaaacaa
     6001 gacaattgtt agcctcggag aggccagtta tagcatatac ctaatccatg ggatagtttt
     6061 attccttatg aagaaagtag acatggcaag taaaattaac actttcttag agattttttt
     6121 attcaactta attttaatca taataacagc ttacgcttca aaacttcttc atttaaaaat
     6181 agagaggcga tggtttaata aagacctatc tattcctaaa gaaagttgac cgactgatac
     6241 caagtgaaat ctagggagaa cctaatgatg gtctccctcc acctttactt tttaaccctc
     6301 caaggcactc tctctttctt tgaatttctt gagagtgtgt cttcttaata tactcttcaa
     6361 agaaacgttc tgtcattttc atgtgggtca acttagctat acttttagtt atccctctaa
     6421 cctcactttc ctcaagagga actgcaaact gcagattata agcgttagct cgctcatagc
     6481 aggcttgcag ccattggtcg tactcaggcc agccctgacg aattgcgcgg tatgcccact
     6541 tccgggtctt gtcgaacagt gtgcagtttc ggcctaagcc atagtcggcg acgatttcct
     6601 tgtcgtattc ggtgttcaga tcgagtgagt cggccagcca gtccagcgtg tatagttccc
     6661 gctgccatac ggctattttc
//
