LOCUS       NZ_CP080114             3108 bp    DNA     circular CON 04-SEP-2023
DEFINITION  Enterobacter asburiae strain WW-19C plasmid pWW19C-8, complete
            sequence.
ACCESSION   NZ_CP080114
VERSION     NZ_CP080114.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18388147
            Assembly: GCF_019428505.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ghiglione,B., Haim,M.S. and Dropa,M.
  TITLE     Characterization of Emerging Pathogens Carrying KPC-2 Gene in IncP-6
            Plasmids Isolated from Urban Sewage in Argentina
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Ghiglione,B., Haim,M.S. and Dropa,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUL-2021) Microbiology, University of Buenos Aires,
            Faculty of Farmacy & Biochemistry, Junin Street 956, Buenos Aires,
            Buenos Aires C1113 AAD, Argentina
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 117X
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/04/2023 11:04:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,234
            CDSs (total)                      :: 5,114
            Genes (coding)                    :: 4,977
            CDSs (with protein)               :: 4,977
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 51 of 137
            Pseudo Genes (incomplete)         :: 91 of 137
            Pseudo Genes (internal stop)      :: 24 of 137
            Pseudo Genes (multiple problems)  :: 24 of 137
            Pseudo Genes (short protein)      :: 1 of 137
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080114.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="WW-19C"
                     /isolation_source="raw sewage"
                     /db_xref="taxon:61645"
                     /plasmid="pWW19C-8"
                     /geo_loc_name="Argentina: Buenos Aires"
                     /lat_lon="34.715694 S 58.505958 W"
                     /collection_date="2018"
                     /collected_by="AySA"
     gene            join(1852..3108,1..96)
                     /locus_tag="KZX48_RS26075"
                     /old_locus_tag="KZX48_26065"
     CDS             join(1852..3108,1..96)
                     /locus_tag="KZX48_RS26075"
                     /old_locus_tag="KZX48_26065"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_219773562.1"
                     /translation="MPNNLNERSRKFQFIHLEAYSLQANQQGGKNAKFNKEVKARTVSE
                     VLGEVLRKDGFCNHIQEPEAPGVLYGDLNNIETDCKAYHQNHKNIDKNGKAKALRSDAN
                     VLIAGVVSLEGIEQNYKDWDQYKIDVLGFLKEKYGENLAAVVEHTDEENPHLHFYIVPK
                     IGQKLDELHDGKKAVLELKKKEPKALKGKQNKAYIEGMRNFQDDFYNKVSKNFGLTKIG
                     PARSRVSRKTYFEQKKAAEEYRKTLKQIEQNKFFIDSEFEQAKKNIENEIKKEKNNFEK
                     EKRKIVKKVVKETYEETMENYENKNYINKMLFSMTYNKTKILNQEKQISHLIKEGRKHF
                     DRKNEYKKEVSRLKDFEDKYTNLRKDFGKRLNNALGEVKVENDNLKMENQKLRSENENL
                     KYENEMGKYAIEFLNKLKNKLGSKFESFKNDLFPKSSHKHTPENPKIKRRF"
                     /name="plasmid recombination protein"
     gene            726..1328
                     /locus_tag="KZX48_RS26080"
                     /old_locus_tag="KZX48_26070"
     CDS             726..1328
                     /locus_tag="KZX48_RS26080"
                     /old_locus_tag="KZX48_26070"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_126513319.1"
                     /translation="MTMKKTKEELTSLFNMYYEIETTEEKLAFARENLFLDKKALSKSF
                     NLITNKEEKLDFIDEYIENSTLRSTTYTENTNQKTSFKNEFVIAFTDNLEKLAFELNVS
                     NAELRVITYILKRMEFGNLISLKQNAIAEKIGMKTANMSVIFKKLKQKKILIEDEDKNL
                     YMNANIFAKGLNHKMKKEKRDSLNRSKISTDIISQSF"
                     /name="hypothetical protein"
     gene            1635..1862
                     /locus_tag="KZX48_RS26085"
                     /old_locus_tag="KZX48_26075"
     CDS             1635..1862
                     /locus_tag="KZX48_RS26085"
                     /old_locus_tag="KZX48_26075"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_126513318.1"
                     /translation="MLKTDLNIFVKKIGKRFIVKIDYVNFESQKHLFLYQEQIKYFFND
                     YLKNVDGNINLFIEENIIKEFFDWEDDYAE"
                     /name="hypothetical protein"
ORIGIN
        1 ttaggatcaa aatttgaaag ttttaaaaat gatttatttc caaaatcttc gcataaacac
       61 acaccagaaa acccaaaaat caaaagaaga ttctgaacat tcgttcgatt ggttcatcgc
      121 ttacgctctt cacgctacgc cccagcccct atggggcgaa ggtcaaaaca ggctttgcag
      181 gaaggggtca caggctctct gtaacgctct ctaagcgttt taacgtttaa gacactaaac
      241 acaatcaggc cgaacggccg gggcggtagc tggtcggctg gatgcttttt gtcgatgatg
      301 ttttgccttt gtccccttgg ggaccaaata aaatccttca ggatttggtt ttgatgtgac
      361 gtttacataa ccgcgagcag ttccacggct tgcccgtggg gctgtgaggg gttggccggg
      421 cggcgagccg aagttgtctt ttctctctta tcttttatta ttttatattt taaaggtctg
      481 ttttttcctt atatatcaac gctttaacta aaaaaaagtt atgaaatttc ataacatcgg
      541 ttatgttttt tcataactct agatatgttt tttcataact gaagttatgg aattttataa
      601 ctctaaaaaa ttggattgaa atccaaaaaa ttagatttta tttaaaaaaa tataacttta
      661 tctattgact ttaatttttt atatcgtata taagtaacaa caaaggaaaa aaagggggtt
      721 ttattatgac gatgaaaaaa acaaaagaag aattaactag tctttttaat atgtattacg
      781 aaattgaaac aacggaagaa aaattagctt ttgcgaggga aaatttattt ttagataaaa
      841 aagcattaag taaaagtttt aatttaataa caaataaaga agaaaaattg gattttatag
      901 atgaatacat tgaaaattca accttaagat ctacgactta tacggaaaac acaaatcaaa
      961 aaacaagttt taaaaatgaa tttgtaattg cttttaccga taatttggaa aaattagcgt
     1021 ttgaattaaa cgtgtcaaat gccgaattgc gtgtaataac ttacatttta aaaaggatgg
     1081 aatttggtaa tttaattagc cttaaacaaa acgcaatcgc ggaaaaaata gggatgaaaa
     1141 ccgccaatat gtccgtaatt ttcaaaaagc taaaacaaaa aaagattttg attgaagatg
     1201 aagataaaaa tttatatatg aacgctaata ttttcgccaa gggtttaaac cacaaaatga
     1261 aaaaagaaaa acgcgattcg ctaaatagat ctaaaatttc aactgatatt atcagccaaa
     1321 gtttctaacg cgctgcgctt gttcaaatcc tataaattat aatcgaaatt ataatttata
     1381 ggatttctca tttaaaaaga cgaagaaaaa acaagcccgc gcagggcttt tttttatgag
     1441 tttttttaag gtgttggcct ttttgttttc cccatttatg gggccgcgct tatacccttt
     1501 agggtataag catagtcggt tcctatgctt ataaattaaa gcaaaattta atcgcaaacc
     1561 caccccagaa aaattgactt ttttttgaaa tatattatta aaattatata aataaaaact
     1621 atataggggg ttatatgtta aaaactgatt taaatatttt tgtaaaaaaa atagggaaaa
     1681 ggtttattgt taaaattgac tatgtgaatt ttgaaagcca aaagcatctt tttttgtatc
     1741 aagaacaaat aaaatatttt tttaatgact atttaaaaaa tgtagatggg aatattaatt
     1801 tatttattga ggaaaatata attaaagaat tttttgattg ggaggatgat tatgccgaat
     1861 aatttaaatg aacgatcaag aaaatttcaa tttattcacc tggaggctta cagcctccag
     1921 gctaaccagc agggcgggaa aaacgctaaa tttaacaaag aggtaaaagc tcgtacagtg
     1981 tccgaggttt tgggtgaggt tttgagaaaa gatgggtttt gtaatcacat ccaggagcca
     2041 gaggctccag gtgtgttata tggcgattta aacaacattg agaccgattg taaggcttac
     2101 caccaaaacc acaaaaatat tgacaagaac ggcaaggcta aagccttgcg ttcggatgct
     2161 aatgttttga tcgctggtgt tgtttcactt gaagggattg agcaaaatta taaagattgg
     2221 gatcagtata aaattgatgt tttagggttt ttgaaagaaa aatacgggga aaatttggcg
     2281 gcagttgtgg aacacacgga cgaagaaaac ccacatctac atttttacat tgtgcccaag
     2341 atcgggcaaa aattagatga attacacgac ggcaaaaaag cagttttaga attaaaaaag
     2401 aaagaaccaa aagccctaaa aggaaaacaa aacaaagcat atattgaagg aatgcgaaat
     2461 tttcaggatg atttttataa taaagtctcc aaaaattttg ggcttactaa aattggtcca
     2521 gcgcgtagcc gcgtaagtcg caaaacgtat tttgagcaaa aaaaagcagc ggaagaatac
     2581 aggaaaacct taaaacaaat agagcaaaat aaatttttta ttgattccga atttgaacaa
     2641 gctaaaaaaa acattgagaa tgaaataaaa aaagaaaaaa ataattttga aaaagagaaa
     2701 aggaaaattg ttaaaaaagt agttaaagaa acatacgaag aaacaatgga gaattatgaa
     2761 aataaaaatt atataaataa aatgcttttc tcgatgactt ataacaaaac taaaattttg
     2821 aaccaagaaa aacaaatttc ccacttgata aaggaaggaa gaaaacattt tgatcgaaaa
     2881 aatgaatata aaaaagaagt ttcaaggcta aaagattttg aagataaata tacaaattta
     2941 agaaaagatt ttggaaaaag gttaaataat gccctcggcg aggttaaagt tgaaaatgat
     3001 aatttaaaaa tggaaaatca aaaattaaga tctgaaaacg aaaatttaaa atatgaaaat
     3061 gagatgggaa aatatgcgat tgagttttta aataaattaa aaaataag
//
