LOCUS       NZ_CP080113             3872 bp    DNA     circular CON 04-SEP-2023
DEFINITION  Enterobacter asburiae strain WW-19C plasmid pWW19C-7, complete
            sequence.
ACCESSION   NZ_CP080113
VERSION     NZ_CP080113.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18388147
            Assembly: GCF_019428505.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Ghiglione,B., Haim,M.S. and Dropa,M.
  TITLE     Characterization of Emerging Pathogens Carrying KPC-2 Gene in IncP-6
            Plasmids Isolated from Urban Sewage in Argentina
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Ghiglione,B., Haim,M.S. and Dropa,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUL-2021) Microbiology, University of Buenos Aires,
            Faculty of Farmacy & Biochemistry, Junin Street 956, Buenos Aires,
            Buenos Aires C1113 AAD, Argentina
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 117X
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/04/2023 11:04:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,234
            CDSs (total)                      :: 5,114
            Genes (coding)                    :: 4,977
            CDSs (with protein)               :: 4,977
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 51 of 137
            Pseudo Genes (incomplete)         :: 91 of 137
            Pseudo Genes (internal stop)      :: 24 of 137
            Pseudo Genes (multiple problems)  :: 24 of 137
            Pseudo Genes (short protein)      :: 1 of 137
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080113.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="WW-19C"
                     /isolation_source="raw sewage"
                     /db_xref="taxon:61645"
                     /plasmid="pWW19C-7"
                     /geo_loc_name="Argentina: Buenos Aires"
                     /lat_lon="34.715694 S 58.505958 W"
                     /collection_date="2018"
                     /collected_by="AySA"
     gene            complement(join(3716..3872,1..188))
                     /gene="mobC"
                     /locus_tag="KZX48_RS26060"
                     /old_locus_tag="KZX48_26050"
     CDS             complement(join(3716..3872,1..188))
                     /gene="mobC"
                     /locus_tag="KZX48_RS26060"
                     /old_locus_tag="KZX48_26050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_219773561.1"
                     /translation="MSYRERYQRKNFISLCLSDEELSEIENIADRLNMKRAAAAREILV
                     TNSKRLKSQIKKNDNSEILFLYSKISNNINQIAKKMNTNLDKFLSGNGEEFSLLIEEIF
                     EDLERLKNDT"
                     /name="mobC"
     gene            complement(470..1873)
                     /locus_tag="KZX48_RS26065"
                     /old_locus_tag="KZX48_26055"
     CDS             complement(470..1873)
                     /locus_tag="KZX48_RS26065"
                     /old_locus_tag="KZX48_26055"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_123652868.1"
                     /translation="MNEKSIDEILAERQLKEFNESKFGQFFNNQKLKEQELTYLNNKAL
                     DIVNEEDLFEIMSLIPQPFITEENKNVDVLSLIYNNFLENTEIPLTLPIFSFLSFLSAF
                     CVKNEITYTIPLSDEKSPLDTWITILAPSGSAKTFSNSQIKKVIPVGIDGKKVIESNFV
                     KPNGPAKFIEELSNLPETKNGENQYGFWIEDEVSQMFKQVEQIGSPLSEIKEYLLKSYD
                     HEKLTRKSKKETIETKNIVLTLLFINTFESYANNISNESMTDGLMRRFNLIYTEKDSER
                     DFTDYSLYNLKKIRDETLQDKISELFNSIKPDQNFTFSDNCFKLYQKYFKIFWHRKYKT
                     ILPEKENFYRTYMMQSWKFAVFYHLILKREGTEIQGDCLVFGMKISLLFANSMKKFFDF
                     KLNKKLTPKKKEKIKVDFTKYIEYLNENTDITLRTFCRKFNLKKADSISILKSLECNNY
                     QHKLIEELE"
                     /name="hypothetical protein"
     gene            complement(2302..3726)
                     /locus_tag="KZX48_RS26070"
                     /old_locus_tag="KZX48_26060"
     CDS             complement(2302..3726)
                     /locus_tag="KZX48_RS26070"
                     /old_locus_tag="KZX48_26060"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_219773560.1"
                     /translation="MIHKHIGGKKNQRSVKNSLDYLLRTKKPEEQKFVEVLSQTNRSDI
                     ENFNEYIKEKNFKNPFITGVLSFEEKDIDPELKTKIMNEFEEMMFSGIDPENRPPVIWI
                     QHKDKNRLELNYTTFNSLMNKKHFQCYYHLSDKKLFNSFCEKINYENNISSVLDVKEHK
                     ERNTLVNTLNNKIPADKKAIVEKLQEDILSKIITEEINNREELISFIQSKNIIINRVRE
                     NAISIKFSKEDKPISLKGDIYEQGRDYKTYRTESTIDHRADQQYVENQLKHHRENFDTE
                     LKKRHSRNRTRFKPTQSKDQKNVEYRVKKANDSKNVRSEIPDNNNVSFRNEPDIDFFHN
                     NKTIEDVSNEQNFTNDKTKPREEEPAKFDLSREIETANTQQQFIRSRVEEVRRNQQQFR
                     KSLDRTQERSGVCRRSLHSIIHNIRRYVRTFSIYTRQNQRRQEIEHRQKIFLEKLKNQQ
                     TQNNQRSVVRSRFKPY"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
ORIGIN
        1 atttcagaat tatcattttt ctttatttgt gattttagtc tctttgagtt tgtgactaat
       61 atttcacgag cagccgctgc tcgtttcata tttaacctat cagcaatatt ttcaatctct
      121 gataattctt catcactcaa acacaatgag ataaaatttt ttctctggta tcgttcacgg
      181 taactcaaat ttaactcctt tgggtttaag gttttgattt tgatttggtt tttaggggat
      241 tgaaggggga acccctcaca agtaaagtaa tacgaagtat tactctaact tgctatctat
      301 tacatagata acaaattaaa accttaagtc aagagcaaaa ctgaatataa tttgaccttt
      361 tataaaaccg tgagaaaata aaaaagcccg tcagggcttt attatttttg aggggttgaa
      421 caagcgaaaa tatatttgaa tatggcacaa tttaaaattc gcttgttaat tactccagtt
      481 cctcaataag tttgtgttga taattattac attcaagact tttcaaaatt gatattgaat
      541 cggctttttt caagttgaat tttctgcaaa atgttctcaa tgtaatatct gtattttcat
      601 ttaaatattc aatatatttt gtgaaatcta cttttatttt ttcttttttc tttggtgtta
      661 gttttttatt taatttgaaa tcaaaaaact ttttcatact gttagcgaat aataaagata
      721 ttttcatacc aaacactaaa caatcgccct gaatttccgt tccttctctc tttaagatca
      781 aatgataaaa tacagcaaat ttccagcttt gcatcatata agttctataa aaattttctt
      841 tctctggtag aattgtttta tattttctat gccaaaaaat cttgaaatat ttttgatata
      901 atttaaaaca attgtctgaa aatgtgaaat tttgatctgg ttttattgag ttgaataatt
      961 cagatatttt gtcctgtaat gtctcatctc ttattttttt caaattatat aatgagtaat
     1021 ctgtgaaatc tctttcactg tctttttctg tataaatgag attaaatctt ctcattaaac
     1081 catctgtcat tgattcattt gaaatgttat tcgcataact ttcaaatgta tttataaata
     1141 acagggttaa aactatattt ttagtttcta ttgtttcttt tttgcttttt cttgttaatt
     1201 tttcatggtc atatgacttt aacaaatatt ctttaatttc agataatgga gatccgattt
     1261 gttctacctg tttaaacatt tgtgaaactt catcttcaat ccaaaaacca tattgatttt
     1321 cgccgttttt ggtttcggga agatttgaaa gttcttcgat aaatttcgca gggccattag
     1381 gttttacgaa atttgactca ataacttttt ttccgtcaat acctactggt ataacctttt
     1441 ttatttgtga atttgaaaaa gttttcgcac ttccagaagg tgccaaaatc gtgatccaag
     1501 tatcaagcgg cgatttttcg tcgctcaatg gaattgtata tgtgatttca tttttgacac
     1561 aaaaagcact taaaaaactt aaaaacgaaa aaataggaag tgttaatggt atttcggtat
     1621 tttctaaaaa gttattatat ataaggctca aaacatcaac atttttattt tcttctgtaa
     1681 taaatggttg aggtattaat gacattattt cgaataaatc ttcttcgttc actatgtcta
     1741 atgctttatt gttaaggtat gttagttctt gttcttttag tttttgatta ttgaaaaact
     1801 gaccaaattt cgattcgtta aattctttta gttgtctttc agctaatatt tcatcaattg
     1861 atttttcatt cataattaat ttctcctatt ttggttatat tacatatata acataggaaa
     1921 aaacattgtc aacggttttt gtgaaaaaac tatgacacgt tttaaacctt gatttatgac
     1981 actatgacac tatgacacaa cagataaagc cttgatacat aaggatttta tcacttttta
     2041 gggttatgac aaaacctatg acaaaagtta tgacacttgt cttaaaatca tagttgagaa
     2101 aaaaagctgt tttttatgtc ataagaaaac gaaaaatatt cagacaggtt cttgtcaaaa
     2161 actccgacaa agttatgaca gaaaaaccgc cctgaccggg caggccaacc cctcacaagc
     2221 aaaaaccagc ctctggctgg ttcttacttg ttcacggtta aggttttgtc gtagtgggtt
     2281 agaagagtgt tcagaacggt tctaatacgg tttaaatcta cttctgacga cacttctttg
     2341 attgttttgt gtttgctggt ttttgagctt ttcaaggaag attttttgtc tgtgttcgat
     2401 ttcttgtctt ctctggttct gtcttgtata gattgaaaac gttctgacgt atcttctaat
     2461 attgtgaata attgaatgta gagatcttct acatactcca gatcgttcct gagttctatc
     2521 aagcgacttt ctgaattgct gttgatttct tctaacctct tcaactcttg aacgaatgaa
     2581 ttgttgttgt gtattagctg tttcaatttc tcttgataga tcaaattttg ctggttcttc
     2641 ttctcttggt tttgttttat catttgtgaa attttgctca ttgcttacat cctcgatagt
     2701 tttattatta tgaaaaaaat caatatcagg ttcattccta aaagaaacat tattattgtc
     2761 tggtatttca gatctaacat ttttactgtc atttgctttt ttaactctgt attcaacatt
     2821 tttctggtct ttgctctgtg ttggcttaaa acgagttcta tttctgctgt gtcttttttt
     2881 aagctctgtg tcaaaatttt ctctatggtg tttgagttgg ttttcgacat actgctgatc
     2941 tgctctgtga tcgattgttg attctgttct ataagttttg taatctctgc cctgttcata
     3001 tatatctcct ttcagtgata ttggtttatc ttcttttgaa aactttattg atatagcatt
     3061 ttctctaact ctatttatta ttatgttttt agattgaatg aatgatatta attcttctct
     3121 gttgtttatt tcttctgtga ttattttcga taaaatatct tcctgtaatt tttcaacaat
     3181 ggctttttta tctgctggta ttttattatt taaagtattt acaagcgtgt ttctttcttt
     3241 gtgttcttta acatctaaaa cagaactgat attattttca taatttattt tttcacaaaa
     3301 tgagttgaat aattttttat cagataaatg ataataacat tgaaaatgtt ttttattcat
     3361 taaggagtta aaagtcgtgt agtttaattc aagtctgttt ttatctttgt gttgtatcca
     3421 aatgaccgga ggtctatttt ctggatctat tcctgaaaac atcatttctt caaattcatt
     3481 cattattttt gtttttagtt ctggatcgat gtctttttct tcaaaagata aaacaccagt
     3541 tatgaaaggg tttttgaagt ttttttcttt tatgtattca ttaaaatttt caatatcaga
     3601 tctgtttgtt tgtgataaaa cttcaacaaa tttctgttct tctggttttt tagttcttaa
     3661 taaataatca agtgaatttt taacacttcg ctgatttttc tttcctccga tatgtttatg
     3721 tatcattttt caacctctct aaatcttcga atatttcttc aatgagtaat gaaaattctt
     3781 ctccgtttcc tgataaaaat ttatcaagat tagtattcat cttttttgcg atctgattta
     3841 tattgtttga gattttagaa tataaaaaca at
//
