LOCUS       NZ_CP080112             4152 bp    DNA     circular CON 04-SEP-2023
DEFINITION  Enterobacter asburiae strain WW-19C plasmid pWW19C-6, complete
            sequence.
ACCESSION   NZ_CP080112
VERSION     NZ_CP080112.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18388147
            Assembly: GCF_019428505.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Ghiglione,B., Haim,M.S. and Dropa,M.
  TITLE     Characterization of Emerging Pathogens Carrying KPC-2 Gene in IncP-6
            Plasmids Isolated from Urban Sewage in Argentina
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Ghiglione,B., Haim,M.S. and Dropa,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUL-2021) Microbiology, University of Buenos Aires,
            Faculty of Farmacy & Biochemistry, Junin Street 956, Buenos Aires,
            Buenos Aires C1113 AAD, Argentina
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 117X
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/04/2023 11:04:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,234
            CDSs (total)                      :: 5,114
            Genes (coding)                    :: 4,977
            CDSs (with protein)               :: 4,977
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 51 of 137
            Pseudo Genes (incomplete)         :: 91 of 137
            Pseudo Genes (internal stop)      :: 24 of 137
            Pseudo Genes (multiple problems)  :: 24 of 137
            Pseudo Genes (short protein)      :: 1 of 137
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080112.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="WW-19C"
                     /isolation_source="raw sewage"
                     /db_xref="taxon:61645"
                     /plasmid="pWW19C-6"
                     /geo_loc_name="Argentina: Buenos Aires"
                     /lat_lon="34.715694 S 58.505958 W"
                     /collection_date="2018"
                     /collected_by="AySA"
     gene            109..1302
                     /gene="mobQ"
                     /locus_tag="KZX48_RS26035"
                     /old_locus_tag="KZX48_26025"
     CDS             109..1302
                     /gene="mobQ"
                     /locus_tag="KZX48_RS26035"
                     /old_locus_tag="KZX48_26025"
                     /inference="COORDINATES: protein motif:HMM:NF041496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_000190114.1"
                     /translation="MATFHISFKKLRRSEGKSSVYLSAYQNREKTKDNRTGATWDYSKK
                     EGFFGSAILSPAGTPAELVKDSGTLWNAVEAGEKRKDAELCRYVDIAIPKELDDGQKKQ
                     IVLDYCQENFVDYGMIADIAFHDLDSHNPHAHVMLTLRTVSPNGFGNKQREWNNRENIE
                     AWRVNWEIIANDRLKKYGHKSRIDSRSLKDQKEEAERKAVFAKTPKEKAKWISKTIELD
                     RTPMTRIHRHQWRKGQKLRAMEQKEKALLLKIANATYLQQTKPPQPEETMKIEKKPLAR
                     SIIDNLKALYKKTQHIFKSKPNVNKNKKEEKEDPQNFVSSYVINEFGEHILKTELETKP
                     KTGTGTGTGGNNGGGKMGSGSGMSPMNQPQPKIEEPEPEPMPEFEDQQPKRKKKFGM"
                     /name="mobQ"
     gene            1841..2695
                     /locus_tag="KZX48_RS26040"
                     /old_locus_tag="KZX48_26030"
     CDS             1841..2695
                     /locus_tag="KZX48_RS26040"
                     /old_locus_tag="KZX48_26030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_219773559.1"
                     /translation="MIIQEEHNIDFFKNQMPNKPYCTNNLDRGLSIRNKAKALEMLYLQ
                     ANQPAIQTCLLFDLDKKNSFYTFEKVGLPIPHFITKTPKTGRCHYGYMLKAGVCKTQQA
                     RLKPLKYAAAVEMAMAKKLKADLGFAGLITKNPLNDHWSPFWSGADLYDLDYLADFVDL
                     EKPQIQEKKSEAYGLGRNVNLFEDLRQYAYRTVLNFKKISTFEKFETELLLKAQGLNTF
                     CNPLNPLQYKELKATARSIARWTWKNFDSHTFSQIQSNRAKKPRKTSVKNEMLNILMTK
                     GAL"
                     /name="replication initiation protein"
     gene            2692..2955
                     /locus_tag="KZX48_RS26045"
                     /old_locus_tag="KZX48_26035"
     CDS             2692..2955
                     /locus_tag="KZX48_RS26045"
                     /old_locus_tag="KZX48_26035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein"
                     /protein_id="WP_219773558.1"
                     /translation="MIINGKKIKAKEIMEMTGRCERTVRKYFSQPREDYEQTAADRRRQ
                     AYELRSQGLKWQQVADKMGCSYHGAVALYRRYVALDMPQNSL"
                     /name="plasmid replication protein"
     gene            complement(3199..3468)
                     /locus_tag="KZX48_RS26050"
                     /old_locus_tag="KZX48_26040"
     CDS             complement(3199..3468)
                     /locus_tag="KZX48_RS26050"
                     /old_locus_tag="KZX48_26040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_001549726.1"
                     /translation="MSKSTAQQIADLQNKLQALRTKKARQDRREETRQKIILGAEVAKV
                     LNSKPEEINKALVLGLLSKINELDEEEKRDFTRIGMDILQQWKS"
                     /name="conjugal transfer protein TraD"
     gene            complement(3470..3850)
                     /locus_tag="KZX48_RS26055"
                     /old_locus_tag="KZX48_26045"
     CDS             complement(3470..3850)
                     /locus_tag="KZX48_RS26055"
                     /old_locus_tag="KZX48_26045"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_213070177.1"
                     /translation="MEYRGLDFEEQEFHDCLNILHKEIVSFLNENNMAMDFFQYPVEDI
                     CRIIFRDKIDLRTAVILDFGITTYDLSPLADHDPFVWRLLEWPLYDYENFQFVIVQKLA
                     SYELDKLNEIIQVEDNKDKRRL"
                     /name="hypothetical protein"
     Typing          110..1119
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.703"
                     /coverage="85.0"
                     /evalue="0.0"
ORIGIN
        1 gccgaaaaaa taatattagt aatgcccact tacacgtcgc aagcgacgta tgggccctgc
       61 ggggctaaaa accaaaattc aaaagacaaa aaaagtaagg ggtagactat ggcgacattt
      121 catatcagct ttaaaaaatt acgaagatca gaaggtaaat cttcggttta tttatctgca
      181 tatcaaaacc gagaaaagac aaaagataat cgaaccgggg caacctggga ttattcaaaa
      241 aaagagggat ttttcggctc tgctatcctc tcccctgccg gcacacctgc cgaactggtg
      301 aaggactcag gcaccctctg gaatgccgta gaggctggcg agaagcgaaa ggatgccgaa
      361 ttatgtcgct atgtggacat agccatcccc aaggagctgg acgacggcca gaagaagcag
      421 atcgtcctcg attactgcca agaaaatttc gtggattatg gaatgattgc agacatagca
      481 tttcatgatc ttgatagtca taaccctcat gctcatgtaa tgctaacttt aagaactgtt
      541 agcccaaatg gatttgggaa caaacaaaga gaatggaata atagagaaaa tattgaagcc
      601 tggcgagtta actgggaaat aatagcaaat gacagattaa aaaaatatgg tcataagtcc
      661 cgtattgatt cccgttcatt aaaagatcaa aaagaagagg cagaaagaaa ggctgttttt
      721 gctaaaactc cgaaagagaa agccaagtgg atttcaaaaa ctattgaact ggacagaacg
      781 ccaatgacca ggatacacag gcaccaatgg cgaaaaggtc aaaaattaag agctatggag
      841 caaaaagaaa aagcattatt attaaaaata gctaatgcaa catacttaca gcaaaccaaa
      901 ccacctcagc cagaggaaac tatgaaaata gagaaaaagc ctcttgcaag gtcaatcatt
      961 gataatttaa aggcattata caaaaagacc cagcatatat ttaaatcaaa acctaatgta
     1021 aataaaaata aaaaagaaga aaaagaagat cctcaaaatt tcgttagtag ttatgtaata
     1081 aatgaatttg gcgaacatat attaaagaca gagttagaaa caaaacctaa aacaggcaca
     1141 ggaaccggaa ccggtggaaa taatggaggc ggaaaaatgg gttcaggctc tggaatgtct
     1201 cctatgaacc aaccacaacc aaaaattgaa gagcctgagc cggaaccaat gccggaattc
     1261 gaagatcagc aacctaagag gaagaagaaa tttggaatgt agtatatagt ggtatatatt
     1321 cctatatagt agtatcactt aatacaactt aatattattt aatactatac tataatatac
     1381 tacattttta gagttgtgat cctcgccggt tctatttccc ctttatgtat tgccacagct
     1441 tcattaactg acttcataag cattttaaaa aatttactat gcttatttct tttccgtttt
     1501 ttctgcactt tctttttttt catatattca ccaaaaaaaa tcgaatcgcc aatttttctt
     1561 cgaacaatac tcttttttct ttttttattg tttttctatc ttcggtaatg cttcgcacca
     1621 tagatctgat agtaatgctt tgctgccgta tgtaaagaac tgccccaact tcgttgggct
     1681 gttatctgat atggttagac gcacacttga caaattttta agatcggtta gaacaggtac
     1741 aggattgaaa aacctataaa aataaaaacc ccaccctctg cgattttgtt ttggcgacag
     1801 aaaacagaga gtagggccag ataaaaagga ctgaaaaaaa atgataatac aagaagaaca
     1861 taacatcgat ttttttaaaa atcaaatgcc taacaagcct tattgtacaa ataacctcga
     1921 tcgggggttg agcataagga acaaggcaaa agccttagaa atgctgtatt tacaggcaaa
     1981 tcagccagca atccaaactt gcttactctt tgatttagat aagaaaaact cattctatac
     2041 atttgagaag gtgggcttac ccatacctca ctttattaca aaaacaccaa agacaggtcg
     2101 ttgccattac gggtacatgc ttaaagccgg tgtttgtaag acacaacaag caaggttgaa
     2161 gccgttgaag tatgccgctg ccgttgaaat ggctatggcg aaaaaactca aagcagatct
     2221 ggggtttgct ggattgatta caaaaaaccc actcaatgat cactggtcgc cgttctggtc
     2281 aggtgctgac ctttacgacc tggattattt agctgatttt gtggacttag aaaaacctca
     2341 aatacaagag aaaaaaagcg aggcttatgg tttaggtcgc aatgttaatt tgtttgaaga
     2401 tttacgccaa tatgcttata gaacggtttt aaatttcaaa aaaatatcca ctttcgagaa
     2461 gttcgaaacc gaattactat taaaggcaca gggcttaaat actttttgca atccactaaa
     2521 cccgttacaa tacaaagagt taaaagcaac agcaagaagc atagctcgtt ggacctggaa
     2581 aaattttgat agccatacat tttcacagat ccaatcaaac agagcaaaga aacctcgaaa
     2641 aacaagtgtt aagaatgaga tgcttaatat attaatgacc aagggggcat tatgatcatt
     2701 aatggtaaaa aaataaaagc aaaagaaata atggaaatga cgggtcgttg tgaaagaaca
     2761 gtaagaaaat atttttcgca acctcgtgaa gattatgaac aaacagcggc tgatcgtcgt
     2821 cgtcaggctt acgaactacg aagccagggg ctgaaatggc aacaggtagc cgacaaaatg
     2881 ggctgttcgt atcatggagc cgttgccctg tatcgtcgtt atgttgcttt ggatatgcct
     2941 caaaacagcc tgtaacgact tctgagaggt ttttccccct aacctaacca aaccctccac
     3001 ccctgggggg atttttcccc ctttccccgt atccgggctt gtgtctggat aggtgaggga
     3061 cactctggta ccaacaaatc aacaaaccca cccagccacc agaggccctc tggcaacctt
     3121 aaaacctcca gcctacggct ggcaaccctt cacaatgaaa aaccagccaa aggcggctgg
     3181 ttcttcactg ttcactgttt acgatttcca ttgctgcaat atatccattc cgatccgtgt
     3241 aaaatctctt ttctcttctt cgtctaattc attgatttta cttaacaagc ctaatacaag
     3301 agctttattt atttcttctg gtttactatt taaaacctta gccacttctg cacccaggat
     3361 tattttttgt cttgtttctt cccttctgtc ctgccttgct ttttttgttc ttaatgcttg
     3421 tagtttattt tgtagatctg cgatctgctg tgctgttgat ttgctcatat tacaacctcc
     3481 ttttatcttt attatcttct acttggatta tttcatttaa tttatctaat tcataagaag
     3541 ccagtttttg aacaatcaca aactggaaat tctcataatc atataatggc cattcaagca
     3601 aacgccaaac aaaaggatcg tgatctgcca aagggcttaa atcataggtt gtaattccaa
     3661 aatctaaaat aacagcagtt ctcagatcta ttttatctcg aaaaattatt ctgcatatat
     3721 cctcaactgg atattgaaaa aaatccatag ccatattatt ttcatttaaa aaagacacta
     3781 tctctttgtg taatatattt aaacagtcgt gaaattcttg ttcttcaaaa tctaatcctc
     3841 tatattccat attaaaaacc tccacactat aagatagtga atgtattacc acatttagta
     3901 cactcaacct ctgtaattgg tccctcttct tttgaatact cctcagtaaa tctaaatata
     3961 tggcgagtgt tttttttaca atgatcacac caattgactt gtatatcagg accgagagtt
     4021 tctttttcca tataacctcc taattattta ttttattatt cattacctcc attatttact
     4081 ttagtcaaat atattttaat atatttatat tgggcgaagc ctgggtttat tactttatta
     4141 ttttgaggtg ca
//
