LOCUS       NZ_CP080111             4935 bp    DNA     circular CON 04-SEP-2023
DEFINITION  Enterobacter asburiae strain WW-19C plasmid pWW19C-5, complete
            sequence.
ACCESSION   NZ_CP080111
VERSION     NZ_CP080111.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18388147
            Assembly: GCF_019428505.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Ghiglione,B., Haim,M.S. and Dropa,M.
  TITLE     Characterization of Emerging Pathogens Carrying KPC-2 Gene in IncP-6
            Plasmids Isolated from Urban Sewage in Argentina
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Ghiglione,B., Haim,M.S. and Dropa,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUL-2021) Microbiology, University of Buenos Aires,
            Faculty of Farmacy & Biochemistry, Junin Street 956, Buenos Aires,
            Buenos Aires C1113 AAD, Argentina
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 117X
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/04/2023 11:04:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,234
            CDSs (total)                      :: 5,114
            Genes (coding)                    :: 4,977
            CDSs (with protein)               :: 4,977
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 51 of 137
            Pseudo Genes (incomplete)         :: 91 of 137
            Pseudo Genes (internal stop)      :: 24 of 137
            Pseudo Genes (multiple problems)  :: 24 of 137
            Pseudo Genes (short protein)      :: 1 of 137
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080111.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="WW-19C"
                     /isolation_source="raw sewage"
                     /db_xref="taxon:61645"
                     /plasmid="pWW19C-5"
                     /geo_loc_name="Argentina: Buenos Aires"
                     /lat_lon="34.715694 S 58.505958 W"
                     /collection_date="2018"
                     /collected_by="AySA"
     gene            join(4481..4935,1..832)
                     /locus_tag="KZX48_RS26010"
                     /old_locus_tag="KZX48_26000"
     CDS             join(4481..4935,1..832)
                     /locus_tag="KZX48_RS26010"
                     /old_locus_tag="KZX48_26000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899670.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_223230178.1"
                     /translation="MFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFLSTMGYR
                     ADEIIGHHHRIFCPPSLVSSPEYSQFWQRLARGESFSGKYLRLAKGNRSVWLEASYIPV
                     SDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEANENFVNA
                     TGYKRDEIIGKHHRLFCAETLYKSDEYRHFWERLNQGEFFSGLFPRLNRQGDPLWFRAT
                     YNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRESAQNGANVIENS
                     ILMIDRIAQGMGAVSTDISRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEAARAGAS
                     GRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMNTRKGVTLMRE
                     AGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            1839..2162
                     /locus_tag="KZX48_RS26015"
                     /old_locus_tag="KZX48_26005"
     CDS             1839..2162
                     /locus_tag="KZX48_RS26015"
                     /old_locus_tag="KZX48_26005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_176490892.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRPLAAWMRQTCLDEKSARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2371..>2805
                     /locus_tag="KZX48_RS26290"
                     /pseudo=""
     CDS             2371..>2805
                     /locus_tag="KZX48_RS26290"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2840..3331
                     /locus_tag="KZX48_RS26025"
                     /old_locus_tag="KZX48_26015"
     CDS             2840..3331
                     /locus_tag="KZX48_RS26025"
                     /old_locus_tag="KZX48_26015"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_047720453.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            3338..3553
                     /locus_tag="KZX48_RS26030"
                     /old_locus_tag="KZX48_26020"
     CDS             3338..3553
                     /locus_tag="KZX48_RS26030"
                     /old_locus_tag="KZX48_26020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          1554..1726
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.276"
                     /coverage="100.0"
                     /evalue="9.21e-81"
     Typing          complement(1083..1626)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.698"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          2153..3651
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.297"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgtgcttgaa gcaaatgaaa atttcgtcaa tgccactggc tataagcggg atgaaatcat
       61 aggtaagcat catcgtctgt tctgtgccga gacgctttac aaaagtgatg aataccgaca
      121 tttctgggag agactgaatc aaggtgagtt tttttctggt ctgtttccac gccttaaccg
      181 tcagggagac cctctgtggt tccgtgcaac ttataaccct gtctttaaca gtgatgggca
      241 gctttataaa attgtgaaat tcgctacaga tgttacggct gatgtcctgc gaaaccagag
      301 agagcaagag gctgctgtgc atgcatggga tatggccgtg cagacccgag aaagtgcgca
      361 gaacggtgcc aatgttattg aaaatagcat ccttatgatt gacagaattg cgcaggggat
      421 gggggctgtc tccaccgata tctcacggct caacaatcag tctgaaagta ttgacgatat
      481 ggtggaaacc atccggaagt ttgccatgca gacaagactt attgcactga atgccgcgat
      541 tgaagcagca agggctggcg catccggaag aagttttgcg gttgttgccg cagaagtgcg
      601 taatcttgcg gcgagcgtca gtagcgcgac tgaagaaatt gagcaagtcg tggccagcaa
      661 tagtcaactg gccaaggatg ttctctgtgg cattgaaaac agcctgatga acactcggaa
      721 aggggttaca ctcatgcgcg aagcgggtga agttatgacc agcattcaga ggaatgctgc
      781 aggggttgag actgcggtta aggatgtcgc catttcagtt aaagccggat aggccatggt
      841 gcatatcccc atatctgtcg aacatcacag atgatcttct tgagatcctt tccgttgcgt
      901 cgtaatctct tgctctgaaa acgaaaaaac cacccggcaa ggtggctttt tcgaaggttc
      961 gcaagagttg acgctctttt gaaccgcggt aactggcttg gaggagcaca gtcaccaaaa
     1021 ctgtcctttc agtgtagcct tatcgcaccc gaaacttcaa gactctcctc ctctaaacct
     1081 gttaccagtg gctgctgcca gtggcgcttt gtcgtgtttt tccggattgg actcaagttg
     1141 atagttaccg gataaggcgc agcggtcgga ctgaacgggg ggttcgtgca tacagtccag
     1201 cttggagcga actgccttcc cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc
     1261 ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc
     1321 caccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac cactgatttg
     1381 agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggcttctccg
     1441 tggccttctc gcttccctgt taagcttctt tctggcatct cccaggaaat ttccgccccg
     1501 cctgtaagcc ggtaccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1561 ggaagcggaa tatatcctgt atcacatatt ctgctgacgc tccgctgctg catttttctc
     1621 ctgccagatg aagcacttca ctgatattcg catccgtgcc aatatagtaa gccagtatac
     1681 actccgctag cgctacgtga ctggttcagg gctgcgcccc gaaacccgcc aaaagcctga
     1741 cgcgcctgcg gcttgtccaa ccccgcgccg accgggaaag gctttcccgc ccgtcccggc
     1801 gttatcagga ggccggtttg tcagagaaac ggaacaagat gctcaccatg tgggtgacgg
     1861 aggacgagca ccggcggctg cttgagcgct gtgacggcag gccgctggcg gcgtggatgc
     1921 ggcagacctg cctggacgaa aagtctgcgc gcaccggaaa gctcccctca atctcgccgg
     1981 cgctgctgcg ccagcttgcc ggcatgggga ataacctgaa ccagatagcc cgccgtgtta
     2041 acgccggcgg cggcaccggc catgacaggg tgcagattgt ggcggcgctg atggccatcg
     2101 atgccgggct tgagcggctg cggcacgccg ttcttgagaa aggaacggac gatgatcgtt
     2161 aaatttcatc cacgtggccg cggcggtggc gccgggccgg tggactatct gctgggtaaa
     2221 gaccggcagc gtgaaggcgc cagcgtcctg caggggaagc cggaagaagt ccgggagctg
     2281 attgacgcgt cgccttacgt gaagaaatac acctccggcg tcctgtcgtt tgcggaggcc
     2341 gatctgcctc ccggccagcg ggaaaaactg atggccagct tcgagcgggt tctgatgccc
     2401 ggacttgata aagatcagta cagcattctg tgggtggagc acgaggacaa ggggcgtctg
     2461 gagctgaact ttctgatccc caataccgag ctgctgaccg gcaggcggct ccagccgtac
     2521 tacgaccgcg ccgaccggcc gcgtatcgat gcctggcaga ccatcgtgaa cggcagactg
     2581 gggctgcacg atccgaacgc gccggaaaac cgccgggcgc tggtcacgcc ggccggactg
     2641 ccgaaaacga agcaggaagc cgcagaagcc atcacccgtg gactgctgag cttagcctcg
     2701 tccggggagc tgaaaagccg tcaggacgtc actgaggctc ttgaaggtgc aggttttgag
     2761 gtggtgcgta caacgaaaag cagtatcagc attgccgacc ctgacggggg gcgaaacatc
     2821 cgactgaagg gagccatcta tgagcagtct tttaacgctg gcgaaggact tagagcagca
     2881 atcgaaagcg cagcagaaga gtaccggcga gatgctgaaa gccgcattca gcgagcacga
     2941 gcagtctgtc agagcggaac tgaacgcaag cgcgaggaga atcagcgacg ccatcagcgc
     3001 ccacgagcag agtatgagcg aggcgatgga gaaaaaccgg cggagcgtgt tgctgacggc
     3061 cgggaggaca tggctcacca tcctgatggt gtcagcgctg ctgatcgcca cgagcgggag
     3121 cattctgtgg tggcaggggc agcagataac cgacaattac acgcatcttc gtcagcagga
     3181 agataccctg gcgaaaatga ccgcccggac gtggggagtg cgctatcagg agagcagcga
     3241 cggccggagg tttcttatcc tgccgccggg gatgcaagcc gaagccatcc cctacgacgg
     3301 gacgacatgg atccgcctga aacaggagta gcggaaaatg acagagctgg aaaagcagtt
     3361 gctgagcgca ttagagcagc tacagcagga ctactcgcaa aggctggacg aatgggagag
     3421 cgccttcgcg gaatggcgga gcatgtgtgg tcttatgcaa cgggagaacg cggtgctgaa
     3481 cgagcgcgtg tcggacttga gcacgcaggt gctgagttta agcgagcagc tgcgccgctt
     3541 gtccgggaac tgaatgccat cgaggagaag gctgcactgg agtatcagcg tcagaacgag
     3601 ctggagctga cgctccgaca gaagcagaag agtttcgatg gtccgaagat gaggtgacag
     3661 cccgcacgaa gaagggctca tcgcgttcct gctaaatagg ctgactaccg gaatggtagt
     3721 gacttatccc ggaggaagtg atgaaaattc tcaacgttat tgaagccctg ctgaactgtt
     3781 tttacctggt gatggagctg tgtgagttgc cggagaggca tttgccggtt tttaaggcgt
     3841 tcgcgctgat tttagtgtga ctttacgctg catgcatctg gctgcatgac tatgcatcag
     3901 tgtaatatcc agcggctgcg tttttggtgt tttttcgtca tgtctgtcgc cactttctcg
     3961 cgtctggcac cggaaacgca gccaaccccg tttctgcggt gcgggcaagg ggtgttatgt
     4021 taaaagccga ggtctggcgg atacggaaaa aattatgccg gccgggctta tgcccgcgga
     4081 aatccggccg atacttaaat tatccttcac aaaggatatc ctgcgatcca gtcagttgag
     4141 tgttttccgt ctcccggatg ggagccattt ttttgccttc tgatgcgtgt taaggcttgc
     4201 ggagctgcgc tggttcttcc aagattgaga gacgccagct tcctgctgga gccggcggat
     4261 ttgccgtaaa tatgaacatt ttcttattgt tcagcccgct aaagatggcg ggttttttac
     4321 gcgtgaaatc cggagccgga catcatgccg gcatggctga agagcgggct ttttcccgcc
     4381 ctgtcgcagt aatatataaa gctacataag gagtagccga taaccatggc agtacttaca
     4441 ttatccgggc aatctgattc atatgttttc acttaaatat atgttctccc tgaacctcca
     4501 gcgatcagcc atttcgtatg acgcaatttg tcagaataag cctgtcattg agttcagtcc
     4561 ggagggggtg ataaataaag ccagcccgct ttttctctcc actatggggt acagggctga
     4621 tgaaataatc ggtcaccacc atcgtatatt ctgcccgccc tcgctggtaa gttcaccgga
     4681 atacagccag ttctggcagc gccttgccag gggagaaagc tttagtggca agtacctgcg
     4741 gctggcaaag ggaaatcgtt ccgtatggct tgaagccagt tatatccccg tttctgacag
     4801 gcgcggccgt gttatcagag tcattaaaat tgctgccgat atttctgagc gcgtgcattc
     4861 tgctcttgag caggaagcag tcgtaaatgc cataaaccgt tctatggcca tcatcacctt
     4921 caatcctgaa gggat
//
