LOCUS       NZ_CP080106             2954 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella quasipneumoniae strain WW-14A plasmid pWW14A-8, complete
            sequence.
ACCESSION   NZ_CP080106
VERSION     NZ_CP080106.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18388146
            Assembly: GCF_019428485.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ghiglione,B., Haim,M.S. and Dropa,M.
  TITLE     Characterization of Emerging Pathogens Carrying KPC-2 Gene in IncP-6
            Plasmids Isolated from Urban Sewage in Argentina
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Ghiglione,B., Haim,M.S. and Dropa,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUL-2021) Microbiology, University of Buenos Aires,
            Faculty of Farmacy & Biochemistry, Junin Street 956, Buenos Aires,
            Buenos Aires C1113 AAD, Argentina
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 82X
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:48:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,986
            CDSs (total)                      :: 5,859
            Genes (coding)                    :: 5,625
            CDSs (with protein)               :: 5,625
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 84 of 234
            Pseudo Genes (incomplete)         :: 159 of 234
            Pseudo Genes (internal stop)      :: 43 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080106.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WW-14A"
                     /isolation_source="raw sewage"
                     /db_xref="taxon:1463165"
                     /plasmid="pWW14A-8"
                     /geo_loc_name="Argentina: Buenos Aires"
                     /lat_lon="34.715694 S 58.505958 W"
                     /collection_date="2018"
                     /collected_by="AySA"
     gene            complement(256..678)
                     /locus_tag="KZX49_RS29750"
                     /old_locus_tag="KZX49_29730"
     CDS             complement(256..678)
                     /locus_tag="KZX49_RS29750"
                     /old_locus_tag="KZX49_29730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_004207907.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKDIKVID
                     VSELIRVFGSLDGVTDTVKYDVKSDAISTEVNTIGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHDPSSPAHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(678..869)
                     /locus_tag="KZX49_RS29755"
                     /old_locus_tag="KZX49_29735"
     CDS             complement(678..869)
                     /locus_tag="KZX49_RS29755"
                     /old_locus_tag="KZX49_29735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004207910.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDSLQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1312..1455
                     /locus_tag="KZX49_RS30305"
     CDS             1312..1455
                     /locus_tag="KZX49_RS30305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_085409091.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDLSPLAAATGNGFAEQRNV
                     EV"
                     /name="replication initiation protein"
     gene            1745..2122
                     /locus_tag="KZX49_RS29760"
                     /old_locus_tag="KZX49_29740"
     CDS             1745..2122
                     /locus_tag="KZX49_RS29760"
                     /old_locus_tag="KZX49_29740"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_110982627.1"
                     /translation="MIHNHPTWVIKSMIYRLGILSISLLTVGCVSTSNVLELGRDTYTV
                     SATADGFRDAASARQSAFKTGSEKCNSLGKKFLLTNENVANTRMGIDTTVTLYFRCLDE
                     RDSEYVRPNLQKAPDLRIESK"
                     /name="hypothetical protein"
     gene            2179..2484
                     /locus_tag="KZX49_RS29765"
                     /old_locus_tag="KZX49_29745"
     CDS             2179..2484
                     /locus_tag="KZX49_RS29765"
                     /old_locus_tag="KZX49_29745"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058876158.1"
                     /translation="MMKVGNVREYGPTKSHLLLMVTTLPAGVGVFIYPIVEVIWGDLSL
                     LKVAGYTFLSLIVGGLLANIIVRGLSATTFGILLVNPLAMICSIAGLIVLAIQHLP"
                     /name="hypothetical protein"
     Typing          1055..1420
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.095"
                     /coverage="85.0"
                     /evalue="1.36e-162"
ORIGIN
        1 cacatatgaa tcgtgggcaa ttcgtcagcg attcgccata gagtcgtcca cgacttggat
       61 ctgcactttg taacacatca ccgcacattt atttttcatt aaaccattgt tgtaaataag
      121 gaatctgcaa aattttaatt ttgcgtgggg tgtgggtatt tttagtggtg aggagcgttc
      181 agagatctgg cgatttttgg gaaggttcag gaactccccg ggatcgccgg agagcaaaac
      241 tgaaacatgg caggatcagg attttttcca gaatttccac cacggagaat gagccggtga
      301 agaggggtcg tgcttgtgct ccaaaagctg catagcctgg cgaagggaat caatatgctc
      361 atcgcgtgct ttaacagccc ctttaagacc atcgttttca gcttcaagta aagcgattct
      421 atgttgtaaa tcatgtaaac caatggtgtt aacctctgta gaaattgcgt cactttttac
      481 atcatatttt acagtatcag ttacaccatc aagagaacca aacaccctaa taagctcgga
      541 tacatcaata actttaatat ccttcccgtg ctttacaaca gaggtataac ttaacttccc
      601 gctgttaatc atcttgtaaa tagtgggacg actaacctta acaagccgtg cagcttcact
      661 aatagacact ctggccatca gttaccatcc tgtaatgaat ccagtctcga ggagagtgta
      721 cggaaaagat gctctgcatc atcgtggagt ttctcacaaa ggtcggcctc agcatcaaga
      781 tcaagctcgt taagcttctc cagcaggagc agagactgat tctggatgaa tttagccatg
      841 ttcagcgccg ttttctgttg tttcgtcata aagggcctca gagcgtcagt gctcagtgta
      901 aaacctgtct cttttccgct tcctcgctca ctcagtcgct acgctccggt cgttcgactg
      961 cggcgagcgg caggagcatg catagaaaag ctcgagaaaa ccgagcggtc aaaggaggaa
     1021 aaggaggaaa aggaggaaaa ggaggaaaaa aaggaaaggc cacggcgttg ccgtttttcc
     1081 ataggctccg cccccctgac aagcatcgca gaaacggacg ctcaaatcag tggtggcgaa
     1141 acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc
     1201 ctgttcctgc ctttcggttt accggtgtca ctccgctgtt atggccgcgt ttatctcatt
     1261 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
     1321 ccccccgttc agtccgactg ctgcgcctta tccggtaact atcgtcttga gtccaatccg
     1381 gaaagacacg acttatcgcc actggcagca gccactggta acggattcgc agaacaaaga
     1441 aatgttgaag tgtgaggtga agtacagcta cactgtaata actaatttgg tttctgtgct
     1501 ctgcgaaagc cagttacctc ggttcaaaga gttgctgctc taagaacctt cgaaaaaacc
     1561 gccttgctgg gcggtttttt cgtttccagg gcaagagatt acgacgcaac agaaaggatc
     1621 tcaagaggat cctattccat ccctctcccc cccaaaggga aaccagcacc actccacgac
     1681 accagttctt tgaagaaaat tattgaaccc cttgatttta ataataaaaa aaacagatat
     1741 aatcatgatc cacaatcacc caacttgggt aataaaatca atgatttata ggttaggtat
     1801 tttatcaata tcgctgctta cagttggttg tgtttctacc agcaacgttc ttgagttagg
     1861 tagggataca tatacggttt cagcaactgc tgatggtttt agagatgcgg catcagcacg
     1921 tcaaagtgcc tttaaaacag gttctgagaa atgtaatagc ctcggtaaaa aattcctgct
     1981 cactaacgaa aacgtggcta acacaagaat ggggatagat accacagtaa cactctactt
     2041 ccgttgtctt gacgaaagag acagcgagta tgtacgacca aatctacaga aagcacctga
     2101 tctacgcatc gagagtaaat aattatgaat ttccttatga tcatgatcct atggtggata
     2161 tcgtcattca tcacacacat gatgaaagtt ggaaatgtca gagagtacgg tcccactaaa
     2221 agccatttac tgttgatggt tactacgctt cctgctggcg ttggtgtttt tatctatccg
     2281 atagtagagg tgatatgggg agatctgtcg ttactgaaag ttgctggtta taccttttta
     2341 tccctcattg ttggcggact tcttgccaat atcattgttc gcggattatc tgcgacaaca
     2401 ttcggcattt tgctcgtaaa tccattagca atgatatgta gcattgccgg actgatagtc
     2461 cttgctatcc aacatttacc ataacatccg ttatcagcac cgcctgagag gccctcagcg
     2521 cgtttctgac gggaaaccgg taaaacaggc aacaaaccac ccgaaaaccc gccagaaacg
     2581 cgcaggctct gtttttacgg catgcatgag tatgcgcgca agtgcatagc gcggaaattc
     2641 acaccattca gccggggtaa gtcctgtttc cggcgttctt cccatccgaa aacggtgaac
     2701 ggggacgcaa tttattgcgt ggccgtgaag cgttgccggc gcagccggaa aaccggtttt
     2761 cagcacgttc aggaatcggg ttcgtcgacg gccggcaatc acgttatggc aagtcaccgg
     2821 acgacttagc acacgattca ccggcgattc gaatcgtgaa ttgaatcaag agtcaccatg
     2881 aacaaaaaac acccaaagtg atgaattgaa tcaagagtcg attcatcata acaatatgat
     2941 aataaagcgt gatt
//
