LOCUS       NZ_CP080105             3478 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella quasipneumoniae strain WW-14A plasmid pWW14A-7, complete
            sequence.
ACCESSION   NZ_CP080105
VERSION     NZ_CP080105.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18388146
            Assembly: GCF_019428485.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Ghiglione,B., Haim,M.S. and Dropa,M.
  TITLE     Characterization of Emerging Pathogens Carrying KPC-2 Gene in IncP-6
            Plasmids Isolated from Urban Sewage in Argentina
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Ghiglione,B., Haim,M.S. and Dropa,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUL-2021) Microbiology, University of Buenos Aires,
            Faculty of Farmacy & Biochemistry, Junin Street 956, Buenos Aires,
            Buenos Aires C1113 AAD, Argentina
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 82X
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:48:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,986
            CDSs (total)                      :: 5,859
            Genes (coding)                    :: 5,625
            CDSs (with protein)               :: 5,625
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 84 of 234
            Pseudo Genes (incomplete)         :: 159 of 234
            Pseudo Genes (internal stop)      :: 43 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080105.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WW-14A"
                     /isolation_source="raw sewage"
                     /db_xref="taxon:1463165"
                     /plasmid="pWW14A-7"
                     /geo_loc_name="Argentina: Buenos Aires"
                     /lat_lon="34.715694 S 58.505958 W"
                     /collection_date="2018"
                     /collected_by="AySA"
     gene            join(3141..3478,1..64)
                     /locus_tag="KZX49_RS29725"
                     /old_locus_tag="KZX49_29705"
     CDS             join(3141..3478,1..64)
                     /locus_tag="KZX49_RS29725"
                     /old_locus_tag="KZX49_29705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_011977838.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGSFEQPKGEHLSGQVSEQRDTAFADQNEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(847..1524)
                     /locus_tag="KZX49_RS29730"
                     /old_locus_tag="KZX49_29710"
     CDS             complement(847..1524)
                     /locus_tag="KZX49_RS29730"
                     /old_locus_tag="KZX49_29710"
                     /inference="COORDINATES: protein motif:HMM:NF025107.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_117058735.1"
                     /translation="MIYKEKKINRNQIPAILASRWGYSILEEAGWTTVPNLLLEKQMVL
                     ELSNTEMLLLIHLISFIHFADTPAFPSISTLAGRINQHPRTVQRTINKLISKGVLKRKI
                     RAKSVNDKGLSNLYSFEPLIEKLLISISNNKDQYEELQKEQGERDLKTVDQLVRIAAAG
                     GGLRLSASTKTVDQLVRIAAAAGNSITPNKAKLVLFDLESKTVDELVRIAAAGKGCVLF
                     ELE"
                     /name="helix-turn-helix domain-containing protein"
     gene            1716..1946
                     /locus_tag="KZX49_RS29735"
                     /old_locus_tag="KZX49_29715"
     CDS             1716..1946
                     /locus_tag="KZX49_RS29735"
                     /old_locus_tag="KZX49_29715"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WP_011977835.1"
                     /translation="MTTAIGKWGNGAALRIPQPFMKQLGLSIGDVVNFRVTERDLVITK
                     AGPTLEELLGQCTAENRHHEYLCDSQGKEML"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            1943..2281
                     /gene="mazF"
                     /locus_tag="KZX49_RS29740"
                     /old_locus_tag="KZX49_29720"
     CDS             1943..2281
                     /gene="mazF"
                     /locus_tag="KZX49_RS29740"
                     /old_locus_tag="KZX49_29720"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="WP_011977836.1"
                     /translation="MTTYCPARGDVILLDFNPQSGHEQAGKRPALVVSDDLFNQVTGFA
                     VVCPITNQIKGYPFEVPVDGTTKTTGVILADQVKSLDWKARAARTVDSVSGETVTTVVD
                     MVSKIIKA"
                     /name="mazF"
     gene            complement(2412..2545)
                     /locus_tag="KZX49_RS30125"
                     /pseudo=""
     CDS             complement(2412..2545)
                     /locus_tag="KZX49_RS30125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            2950..3141
                     /locus_tag="KZX49_RS29745"
                     /old_locus_tag="KZX49_29725"
     CDS             2950..3141
                     /locus_tag="KZX49_RS29745"
                     /old_locus_tag="KZX49_29725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_011977837.1"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(2445..2902)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.5"
                     /coverage="90.0"
                     /evalue="1.69e-147"
ORIGIN
        1 aaatcaccag gcaaaattaa ctgaatcccc ggcgccatgg tggaaattct ggaaaaagtc
       61 ctgacaccgt catgcttcag ttttcctctc cggccattcc ggggagttcc tgaaccttcc
      121 caaaaatcgt cagatctctg aacgcacctc accacttaaa atacccacac accacgcaaa
      181 attaaaattt tgcagataac tcattaatat cagtttgtta ggtgttatta aattacgaat
      241 tattaccttc tgtaaggtaa atccaagtcg ccggcaagtc gtatacgact tgccggcgac
      301 ttgtcataga gtcatcgaca agtcactcca gactatcagt aacttatgac gacttgactc
      361 tagagttgag tcgttcattt ggtgtttttt tgaccttggt gactctagag tcaagtcacg
      421 agtcgaatcg ccggtgaatc gtgtgctaag tcgtccggtg acttgccatc acgtcattgc
      481 cggctgtcgc tgaactggat tcttgaacgt gctgaaaacg ggttttccgg ctgcgccggc
      541 aacgcctcac ggccacgcaa taaattgcgt ccccgttcac cgttttcgga tcagaaaaac
      601 gccggaaaca ggacttaccc tggctgaatg gcatgaattc ccgcgctatg cacttgcatg
      661 catactcatg tatgccgtaa aaacagcccc tgcgcgtttc tggcaggttt tcgggtggtt
      721 tgttgcctgt tttgccggtt tcccgtcaga aacgccctga agccgtttta gcggtgcacg
      781 taatagggct ttatgttaaa ttaagactgg tttgatgttt aaacttatag tgatcttttt
      841 agtgtgttac tccaattcga atagtacaca cccctttcct gctgcagcaa ttcgaactag
      901 ctcatcaaca gtcttggact caagatcaaa cagaactaac tttgctttat tgggggttat
      961 cgagttacct gcggctgcgg caatccgcac gagctgatcc actgtttttg tggatgcact
     1021 taaacgtaaa ccacctcctg ctgcagcaat ccgaactaat tgatctactg ttttcaagtc
     1081 tctttctcct tgttctttct gaagttcttc atactggtct ttgttgttac taattgatat
     1141 taatagcttt tctatcaaag gttcaaagct gtaaagattg ctcaagcctt tgtcattgac
     1201 tgattttgcg cggatttttc tcttcaagac ccctttagat attaatttgt ttatggttcg
     1261 ctgcacggtt cttgggtgtt ggttgatcct acctgctagt gtagaaatgc ttgggaatgc
     1321 tggggtgtct gcaaagtgaa tgaaagagat tagatgaatg agtaacagca tttcggtgtt
     1381 actcaattct agaaccatct gtttttcaag caataaatta ggaacagttg tccaccctgc
     1441 ttcctctagg atgctatatc cccacctgct tgctaagatc gctggtattt ggttgcggtt
     1501 tatttttttt tctttgtaaa tcaatttgtt atcgccactt agttaaaaaa aatttttttt
     1561 tgccattggt aactacttaa acaaataagt atttatttgt ttaagtagtt accccttttt
     1621 agggtggcat tggtgccacc cttcaaactg aactcaaaga ctatacaata caaatattgt
     1681 ttacacattg tttatccatc tgctttggag gtattatgac tacggctatt ggtaaatggg
     1741 gtaatggcgc tgctttgcgt atcccgcagc cgttcatgaa gcagcttggg cttagtatcg
     1801 gtgatgttgt taatttccgc gtcactgaaa gggatttagt aattactaaa gctggcccga
     1861 cgcttgaaga actgctcgga cagtgcactg cagaaaatcg ccatcacgag tatctatgtg
     1921 actctcaagg taaggagatg ctgtgacgac atattgtcca gccagaggcg atgtcattct
     1981 tttagatttt aaccctcaat cagggcatga gcaggcaggg aaaaggcctg ctcttgtcgt
     2041 ttctgatgat ttgtttaacc aagtcactgg gtttgctgtt gtttgcccca taactaatca
     2101 gataaaaggg tacccttttg aagttcctgt tgatgggact actaaaacaa caggggttat
     2161 cttggctgat caagtgaagt ctttagactg gaaggccaga gcggcacgta ctgttgatag
     2221 cgtttccggc gagacagtaa ctacagttgt tgatatggtt tcaaaaatta ttaaagcata
     2281 aaaaaagacc ggcataagcc ggtcttttca ggccgaaaat gttaccagca ttttcggcct
     2341 tcggtgactg ctctaaaggg gaacagtaac caatattgtt tatcaagtgt aactgcttac
     2401 tgttacagca atcaagattt tctttccccc gtagagcgac cgatggctac tgccaatggc
     2461 gctttggcgt gcttgttcgg gttggactca agttgatagt taccgaatta gcgcagtagt
     2521 cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc taaccggaac
     2581 tgagtgtcag gcgtggaatg agataaacgc ggccataaca gcggaatgac accggtaaac
     2641 cgaaaggcgg gaacaggaga gcgcacgagg gagccatcag ggggaaacgc ctggtatctt
     2701 tatagtcctg tcgggtttcg ccaccactga tttgagcgtc aaattttgtg atgcttgtca
     2761 ggggggcgga gcctatggaa aaacggccgt tgtgcggcct tgtctctctt cgtgctgtct
     2821 gtcctatgcc gttatgtgtg cgttgcgacc gctcgccgca gccgaacggc aggagcgtag
     2881 cgactgagtg agcgaggaag cggaaaagag actggtttta cactgagcac tgacgctctg
     2941 aggcttctta tgacgaaaca acagaaaaca gcgctgaaca tggctaaatt catccagcat
     3001 cagtctctgc tcctgctgga gaagcttaac gagcttgatc ttgatgctga ggccgacctt
     3061 tgtgagaaac tccatgatga tgcggagcat cttttccgta cactgtcctc gagactggat
     3121 gcattacagg atggtaactg atggccagag tgtctattag tgaggcagcc cgattgacgg
     3181 gtaagagtag aacaacactg cacaggttaa ttaaagcagg tgacctgtcc acctgtagcg
     3241 gtgaacgtaa tgcaaaaatg gtagatacgt cagagctttt gcgtgtcttt ggttcctttg
     3301 agcaacctaa gggtgaacat ctttctggac aggtctctga acagcgtgat acagcctttg
     3361 ctgaccagaa tgaacaggtt attcgtcatc tgaaacagga aattgaacac ctgaacacct
     3421 tacttttgtc caaggattcg cacattgaca gcctgaaaca ggccatgctc cttcttga
//
