LOCUS       NZ_CP080104             8819 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella quasipneumoniae strain WW-14A plasmid pWW14A-6, complete
            sequence.
ACCESSION   NZ_CP080104
VERSION     NZ_CP080104.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18388146
            Assembly: GCF_019428485.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8819)
  AUTHORS   Ghiglione,B., Haim,M.S. and Dropa,M.
  TITLE     Characterization of Emerging Pathogens Carrying KPC-2 Gene in IncP-6
            Plasmids Isolated from Urban Sewage in Argentina
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8819)
  AUTHORS   Ghiglione,B., Haim,M.S. and Dropa,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUL-2021) Microbiology, University of Buenos Aires,
            Faculty of Farmacy & Biochemistry, Junin Street 956, Buenos Aires,
            Buenos Aires C1113 AAD, Argentina
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 82X
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:48:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,986
            CDSs (total)                      :: 5,859
            Genes (coding)                    :: 5,625
            CDSs (with protein)               :: 5,625
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 84 of 234
            Pseudo Genes (incomplete)         :: 159 of 234
            Pseudo Genes (internal stop)      :: 43 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080104.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8819
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WW-14A"
                     /isolation_source="raw sewage"
                     /db_xref="taxon:1463165"
                     /plasmid="pWW14A-6"
                     /geo_loc_name="Argentina: Buenos Aires"
                     /lat_lon="34.715694 S 58.505958 W"
                     /collection_date="2018"
                     /collected_by="AySA"
     gene            complement(join(8634..8819,1..210))
                     /locus_tag="KZX49_RS30185"
     CDS             complement(join(8634..8819,1..210))
                     /locus_tag="KZX49_RS30185"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_000634405.1"
                     /translation="MIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLA
                     TLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLG
                     RKSLSFSKSVELHDKVIGHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            complement(742..1749)
                     /locus_tag="KZX49_RS29660"
                     /old_locus_tag="KZX49_29640"
     CDS             complement(742..1749)
                     /locus_tag="KZX49_RS29660"
                     /old_locus_tag="KZX49_29640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131685.1"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_065808204.1"
                     /translation="MNIKSALLSAAMAAVSVNCFADTPAPDRHVQQFLDALNGGGGKPI
                     EQLSPQAARQVLIDAQKGATLPAADVSEKTISVDGLNITLKIVKPHNAQGTLPVFMFFH
                     GGGWILGDFQTHERLIRDLVSQSGAAAVYVNYTPSPEAHFPVAINQAYAATRWVAENGS
                     EIGVDGKRLALVGNSVGGNMVAAVALQAKQHKSPAIRYNVMLWPVTDANFDTGSYNQYQ
                     NGYFLSRNMMKWFWDAYTTKESERNNILASPLRATREQLKGLPPTLIQTAEFDVLRDEG
                     EAFGRKLDAAGVDVTVTRYNGLIHDFGLLNALSDVPAVRAAIGQAAGQLKEHLK"
                     /name="alpha/beta hydrolase"
     gene            complement(1966..2733)
                     /locus_tag="KZX49_RS29665"
                     /old_locus_tag="KZX49_29645"
     CDS             complement(1966..2733)
                     /locus_tag="KZX49_RS29665"
                     /old_locus_tag="KZX49_29645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020050724.1"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_065808205.1"
                     /translation="MSKFAASLAIATALLSASAVAETAKPTVVLVHGAFADSSSWNGVT
                     RILQKDGYNVVAAANPLRSVSTDAAYISSVVNNIKGPVVLVGHSYGGQVITNAARETKN
                     VKSLVYVAAFAPDAGEAAAELAGKFPGSTLGEALAAPVKLADGNVDLSIEQSKFHQQFA
                     HDVSAENAALMAAGQRPVTEAALTEKSGAPAWKTLPSYFIYGDSDKNIPVKSLRFMAER
                     AGSKHTVVIKGASHVVMVSHPQDVATLIEEAAK"
                     /name="alpha/beta hydrolase"
     gene            2886..3353
                     /locus_tag="KZX49_RS29670"
                     /old_locus_tag="KZX49_29650"
     CDS             2886..3353
                     /locus_tag="KZX49_RS29670"
                     /old_locus_tag="KZX49_29650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014884107.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_065813429.1"
                     /translation="MTKDINTGDLLPDVHGMLCFSLYSAGHAFTQLYRPMLDKLGLTYP
                     QYLVMVTLWNQDGRTVKELSGILFLESSTLTPLLKRLETAGLITRTRNPKDEREVLIHL
                     TEKGNRLKIEASNITRCIVDTLEMDADVVADIKASVDKIRDQIHRAHKPDI"
                     /name="MarR family transcriptional regulator"
     gene            complement(3535..3684)
                     /locus_tag="KZX49_RS29680"
                     /old_locus_tag="KZX49_29660"
     CDS             complement(3535..3684)
                     /locus_tag="KZX49_RS29680"
                     /old_locus_tag="KZX49_29660"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_213928782.1"
                     /translation="MPTPEVKPAPLRDTNYQNKIIELIDFNKKKNRYNHDPQSPNLGNE
                     INDL"
                     /name="hypothetical protein"
     gene            complement(3890..4021)
                     /locus_tag="KZX49_RS30115"
     CDS             complement(3890..4021)
                     /locus_tag="KZX49_RS30115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_047741455.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGKQDVREM"
                     /name="hypothetical protein"
     gene            4431..4622
                     /locus_tag="KZX49_RS29685"
                     /old_locus_tag="KZX49_29665"
     CDS             4431..4622
                     /locus_tag="KZX49_RS29685"
                     /old_locus_tag="KZX49_29665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_016947206.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSLRLDALQEGD"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4622..5020
                     /locus_tag="KZX49_RS29690"
                     /old_locus_tag="KZX49_29670"
     CDS             4622..5020
                     /locus_tag="KZX49_RS29690"
                     /old_locus_tag="KZX49_29670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_219804397.1"
                     /translation="MAKVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLETSE
                     LLRVFGAFAPVPVEQRGEQVSGQRDTVTSDHNEQVIHTLKQEIEHLNTLLASKDSHIDS
                     LKQAMLLLENREDQRQNTTRPWWRFWQS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(5027..5725)
                     /locus_tag="KZX49_RS29695"
                     /old_locus_tag="KZX49_29675"
     CDS             complement(5027..5725)
                     /locus_tag="KZX49_RS29695"
                     /old_locus_tag="KZX49_29675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_219804398.1"
                     /translation="MTRTFILLCTGVFAIAYALYELNHYGVYYPDVSNPEAFTGWKAVT
                     ELGPAILATAIAATAFSLWLFILFFRGSESRRIQREISGYQQALREAEEAHRARQNTLI
                     QNHNLETAALRSHYIGAEDVLREAFAQKESLLLQAVQNARKEARKELEEQREALNQRES
                     ILQKKELALETTLEKARKYVAGERHNTELARLEAEKAEERRRNACATAERRRRKLEKLQ
                     ATGGTAGQKH"
                     /name="hypothetical protein"
     gene            complement(5722..6609)
                     /locus_tag="KZX49_RS29700"
                     /old_locus_tag="KZX49_29680"
     CDS             complement(5722..6609)
                     /locus_tag="KZX49_RS29700"
                     /old_locus_tag="KZX49_29680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_121567987.1"
                     /translation="MAKFASAARQAAGVIKPLQGNILRSVGTVRNYEQGLTRVAEWMKA
                     NRINHGDLRTLTPAQAITYLEQRGEQVGQKTLDMERQAIQAMMIHVTQTLPAGEHLPVV
                     KSEQAQILSSRAYSPDQVALIAAAQQPANALATAIALAAGLRAHELLTLAPRQERAADP
                     RPAADSKFAGREGVIYTVQGKGGLVREVLLPSPLAERLEHVRLASPVRVTDRGVFYQSQ
                     YAIGGGQRWSNAFSAASMRTLGWSRGAHGVRHSYAQQRMQELQKLGLVRDMALRTVSQE
                     MGHFRPEITETYLR"
                     /name="site-specific integrase"
     gene            complement(6602..6823)
                     /locus_tag="KZX49_RS29705"
                     /old_locus_tag="KZX49_29685"
     CDS             complement(6602..6823)
                     /locus_tag="KZX49_RS29705"
                     /old_locus_tag="KZX49_29685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016947202.1"
                     /translation="MQFDTAAELAALQAQTRRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLRARRIKVVLSTVTRWLEKNG"
                     /name="hypothetical protein"
     gene            7939..8184
                     /locus_tag="KZX49_RS29710"
                     /old_locus_tag="KZX49_29690"
     CDS             7939..8184
                     /locus_tag="KZX49_RS29710"
                     /old_locus_tag="KZX49_29690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012848468.1"
                     /GO_component="GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0097351 - toxin sequestering activity [Evidence IEA]"
                     /GO_process="GO:0045927 - positive regulation of growth
                     [Evidence IEA]; GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="WP_047359280.1"
                     /translation="MHAITFTDARKHFADTMKRVTDDAEPVRVMRRDAPDIVMIDAGEY
                     EALIETVYLFSNPANAAHINESMKQAERGEFVEVEY"
                     /name="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            8165..8440
                     /locus_tag="KZX49_RS29715"
                     /old_locus_tag="KZX49_29695"
     CDS             8165..8440
                     /locus_tag="KZX49_RS29715"
                     /old_locus_tag="KZX49_29695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02116.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="WP_072058806.1"
                     /translation="MSKWNIKFTKNGADDINYWRGTDKKKFERIRQLLHSIESDPLTGI
                     GKPERLRHHKDPALYSRRIDQSHRLVYSIQNGEIIIYAARYHYGDK"
                     /name="Txe/YoeB family addiction module toxin"
     gene            8517..>8615
                     /locus_tag="KZX49_RS30120"
                     /pseudo=""
     CDS             8517..>8615
                     /locus_tag="KZX49_RS30120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /name="DnaJ domain-containing protein"
     Typing          complement(3913..4384)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.586"
                     /coverage="91.0"
                     /evalue="2.1e-176"
ORIGIN
        1 atacagcggc cagccatccg tcatccatac cacgacctca aaggccgaca gcaggctcag
       61 aagacgctcc agtgtggcca gagtgcgttc accgaagacg tgcgccacaa ccgtcctccg
      121 tatcctgtca tacgcgtaaa acagccagcg ctgacgtgat ttagcaccga cgtagcccca
      181 ctgttcgtcc atttcagcgc agacaatcac atcactgccc ggttgtatgc gcgaggttac
      241 cgactgcggc ctgagttttt taagtgacgt aaaaccgtgt tgaggccaac gcccataatg
      301 cgtgcactgg cgcgacatcc gacgccattc atggccatat caatgatttt ctggtgcgta
      361 ccgggctgag aggcggtgta agtgaactgt agttgccatg ttttacggca atgagagcag
      421 agatagcgct gatgtccggc agtgcttttg ccgttacgca ccacgccttc agtagcggag
      481 caggaaggac atctgatgga aatggaagcc acgcaagcac cttaaaatca ccatcataca
      541 ctaaatcagt aagttggcag cattacccgg atacgaacaa ggggaacccc cccccgggga
      601 tgcggacact cccatacctc tcgggggaaa actaaaaaaa ctgaaggcgc tgtcccggca
      661 aatccggaac ctaatcagga tgacaagcag atggccgagg gttcagcgac cagccggcgg
      721 taccggctgg tcacttgttc gttatttcag atgttctttc agctggccgg cggcctggcc
      781 tatggctgcc cgtacggcag gaacgtcact cagcgcattc agcaaaccaa aatcatggat
      841 caagccgtta taccgggtca ccgtcacatc cactcccgct gcgtcgagct tacgtccgaa
      901 cgcttctccc tcatcacgaa gcacgtcaaa ctcggcagtc tggatcagtg tgggtggtaa
      961 gcccttcagc tgctcacgcg ttgcacgcag tggtgaggcg agaatattat tacgctcgct
     1021 ctctttggtc gtataggcat cccagaacca cttcatcatg ttccgtgaca ggaaataacc
     1081 gttttggtac tgattataag aaccggtatc aaaatttgcg tcagtgacag gccataacat
     1141 gacattgtag cgaatggcag gtgacttatg ctgttttgcc tgaagcgcta cggcggcaac
     1201 catgttacct cccacgctgt taccgacaag ggctaaccgt ttgccatcca cgccaatctc
     1261 gctgccgttt tcagccaccc agcgggtagc agcatatgcc tgattgatag caaccgggaa
     1321 gtgcgcttct ggtgacgggg tgtagttgac atagaccgct gctgctccgg actgactgac
     1381 caggtcacga ataagacgct catgtgtctg gaaatcgccc agtatccagc caccaccgtg
     1441 gaagaacatg aaaacaggca gagtgccttg tgcgttgtga ggcttcacaa ttttcagcgt
     1501 aatattcagg ccatcaacac tgatcgtttt ttcgctgacg tccgctgccg gtagcgtggc
     1561 gcctttctgc gcatcaatca acacctgacg tgcggcctgg ggagataatt gttcgatggg
     1621 ctttcccccg cctccgttta atgcatccag gaattgctgg acatggcgat ccggtgcagg
     1681 tgtatcagca aaacagttga ctgaaacagc tgccatcgcg gcagacagta atgccgattt
     1741 gatattcatt gtgttacctc attaacgttg cttgcaatta aattgcaaac tattcatatg
     1801 attaagaaat aatctgtatc aacactttaa taaacattcc tgtcatctcc catgtgaatg
     1861 ccatctcacc agtaaggggt attcacatca ccgaaacagg caggggcgta agggaaaaag
     1921 acagggcccc tgcgggaaat ggagttaaag acaggacaaa gacgtttatt ttgctgcttc
     1981 ctcaataagt gtcgctacgt cctgaggatg agaaaccatc acgacatggg atgccccttt
     2041 gatcacaacg gtgtgttttg atcccgcgcg ttcagccatg aaacgcaaac ttttgacagg
     2101 gatatttttg tcgctgtcac cgtaaatgaa gtaagaaggc agtgttttcc aggcgggagc
     2161 ccctgatttt tcagttaatg ctgcttcggt cacaggtctt tgtcctgccg ccatcaatgc
     2221 agcattttca gctgacacgt catgagcgaa ttgctgatgg aacttcgact gctcaatgga
     2281 cagatcgacg tttccatcag ccagcttaac cggtgcggca agcgcttcgc caagcgtgct
     2341 tccggggaat ttccccgcca gttcagcggc cgcttcaccc gcgtcaggag caaatgcggc
     2401 gacataaacc agtgacttaa cattttttgt ttcccgggct gcattcgtta ttacctgacc
     2461 accataggaa tgcccgacaa gtacaacggg tcccttaata ttgttcacaa cagaagagat
     2521 gtaagccgca tcagttgaaa cactgcgcaa tggattagcc gctgcgacga cgttataccc
     2581 gtctttttgc aggatccggg tgacaccatt ccagctagaa gagtcagcaa aggcaccatg
     2641 gaccaggaca acagtgggct ttgcggtttc ggcgacagca ctggcagaca gcagagcggt
     2701 ggcaatggcg agggaggcag caaatttaga cataatagtt tctctcttca acggttgtat
     2761 gttcgtgcgc aatttgtttg tgcgcgattt gtttgtatgc aaattaagat agtgctcctt
     2821 tttgtcgttg tcaatcgctt gcaattaaat cgcacacaaa ttattttaga ggcactggag
     2881 gacatatgac taaagacatc aataccggag atttactccc ggatgtgcac ggcatgctct
     2941 gcttctctct ctactccgcg ggacatgctt ttacgcagct ctatcgtcca atgctcgata
     3001 agctgggatt aacctatccc cagtacctgg tgatggtcac gttgtggaat caggatggac
     3061 gtactgtgaa ggaactgagt ggaatactct tccttgagtc cagcacgctg actcctctac
     3121 tcaagcggct ggagacggcg ggcttgatca cccggacacg caatccgaag gatgaacgtg
     3181 aagtgcttat tcatctcaca gagaagggga acagattgaa aattgaggcc agcaatatca
     3241 cgcgctgtat tgtggatacc cttgagatgg atgcggatgt ggttgctgac atcaaagcct
     3301 cagtcgataa gatccgtgat cagatacatc gtgcacataa accggatatc tgatgatgct
     3361 ataaagcaat atggccctac ataacattaa gttatagggg cgtatggagc tgcatgacaa
     3421 ggtcatcggg cattatctga acataaaaca ctatcagtaa gttggagtca ttaccgaaca
     3481 ttgctggtag aaacacaacc aactacgagt agcgatattg ataaaattac taatctataa
     3541 atcattgatt tcattaccca agttgggtga ttgtggatca tgattatatc tgtttttttt
     3601 cttattaaaa tcaatgagtt caataatttt attttgatag ttggtgtcgc ggagtggtgc
     3661 tggtttcact tcaggggtag gcatatggaa caggatcctc ttgagatcct ttctttggca
     3721 tcgtaatctc ttgctcagta aacgaaaaaa ccaccctggc aggtggtttt tcgaaggtta
     3781 gttaatcctg gcagattttc taaccgcggt aacagtcttg tgcgagacat gtcaccaaat
     3841 ctgtcctttc agtgtagccg cactcaggcc atcacttcaa gaactcttac tacatctctc
     3901 gcacatcctg tttaccagtg gctgctgcca gtggcgcttt ggcgtgtctt tccgggttgg
     3961 actcaagacg atagttaccg gataaggcgc agcagtcgga ctgaacgggg ggttcgtgca
     4021 tacagtccag cttggagcga actgcctacc cggaactgag tgtcaggcgt ggaatgagat
     4081 aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc
     4141 acgagggagc caccaggggg aaacgcctgg tatctttaag tcctgtcggg tttcgccacc
     4201 gctgatttga gcgtccgttt ctgtgatgct tgtcaggggg gcggagccta tggaaaaacg
     4261 gcaacgccgt ggctttttcc acaggctctt ttcttcccgg tgatagcgat gactcttctg
     4321 tgtgtcccgt ccgctcgccg cagccgaacg gcaggagcgt agcgactgag tgagcgagga
     4381 agcggaaaag agacaggttt tacactgagc actgacgctc tgaggccctt atgactaaac
     4441 aacagaaaac tgccctgaac atggctaaat tcatccagaa tcagtccctg ctcctgctgg
     4501 agaagcttaa tgagcttgat ctcgatgcgg aggctgacct ttgtgagaaa ctccacgatg
     4561 atgcggagca tcttttccgt acactgtccc tcagactgga tgcactacag gagggtgact
     4621 gatggcgaaa gtgtcgatca gtgaggctgc acgcctgacg ggtaagagca ggacaaccct
     4681 gcacaggtta attaaagccg gtgaactgtc cacctgtcgg ggtgaacgga atgcgaaaat
     4741 gctcgagact tcagagcttt tgcgggtgtt tggtgccttt gcgcctgtac ccgttgaaca
     4801 gaggggtgaa caggtcagtg gacagcgtga cacagtcact tctgaccata atgaacaggt
     4861 cattcatact ctgaaacagg aaattgaaca cctgaacaca cttcttgcct caaaagattc
     4921 acatatcgac agcctgaaac aggcgatgtt gttgctggag aaccgggaag atcagcggca
     4981 gaacacgacc cggccctggt ggcgtttctg gcagtcctga aatgcctcag tgtttttgcc
     5041 ctgctgttcc gccagtggcc tggagttttt ccagtttccg tctgcgccgt tccgccgtcg
     5101 cgcaggcatt tctccgtctt tcctctgctt tctctgcttc cagacgggcc agctcggtgt
     5161 tatgccgctc cccggccaca tatttccggg ccttctccag ggttgtttcc agcgccagct
     5221 cctttttctg caggatggat tcgcgctgat tcagtgcctc cctctgttct tccagctcct
     5281 ttctggcttc tttccgggca ttctgcaccg cctgaagcaa gaggctttct ttttgcgcaa
     5341 acgcttcccg cagtacatcc tcagcaccaa tgtagtggct tcgcagggcc gctgtctcca
     5401 ggttgtgatt ctgaataagg gtattttgtc tggctctgtg agcctcttct gcctccctca
     5461 gtgcctgctg atatccgctt atctcgcgct gtatccggcg ggactctgag cctctgaaaa
     5521 acaaaatgaa cagccacagc gagaacgctg tggctgcgat cgcggtagcc aggatagcgg
     5581 gaccgagttc ggtgaccgcc ttccagccag taaacgcctc aggattgctg acgtccggat
     5641 agtaaacgcc atagtgattc agctcataca gggcgtacgc gatggcgaaa acaccggtac
     5701 acaataaaat aaatgttctt gtcatcgcag gtatgtctcc gtgatttccg gacgaaaatg
     5761 gcccatttcc tggctcacgg tacgcagggc catatcacga accagaccga gtttttgcag
     5821 ctcctgcatc cgttgctggg cgtagctgtg acgcacgccg tgtgcccccc gtgaccagcc
     5881 cagggtacgc atggaggcgg cactgaaggc gtttgaccag cgctggcctc cgccaatggc
     5941 gtactgactc tggtaaaaca cgccgcgatc ggtcacccgg accgggctgg ccagcctgac
     6001 atgctcgaga cgttcagcca ggggggacgg cagcagaact tccctgacca ggccgccttt
     6061 gccctgaacg gtgtaaatca caccctcccg accggcaaat ttactgtccg ctgccggacg
     6121 gggatccgct gctcgctcct ggcggggcgc gagggtcagc agttcatggg cgcgtaatcc
     6181 ggcggccagg gcaatcgcgg tggccagagc gttggcgggc tgttgtgctg cggcaatcag
     6241 ggccacctgg tccgggctgt aggcccggct gctcaggatc tgtgcctgct ccgatttcac
     6301 caccggcagg tgttcgccgg cagggagggt ctgcgtcacg tggatcatca tggcctggat
     6361 ggcctggcgt tccatatcga gcgttttctg gccaacctgc tccccgcgct gctccaggta
     6421 ggtgatggcc tgcgccggtg tcagtgtgcg taaatcgccg tgattaatcc ggttggcttt
     6481 catccactct gcgacgcggg tcagtccctg ctcgtaattc cggactgtgc cgacagagcg
     6541 cagaatattt ccctggagtg gttttatcac gccagccgcc tggcgggcag cgctggcgaa
     6601 tttagccatt tttttccagc cagcgggtaa ccgtggacaa caccaccttg attcgccggg
     6661 cccggagcca gcgttgcagc tccgctgcac tggccccggc ctggtacagg ctcagcagtt
     6721 cgccggtata gcggtcaagg cgggagggac ggtagcggac ctggcggata cggcgggtct
     6781 gcgcctgcag ggcggccagt tcggcagcgg tatcaaattg catctgtgct ccggattggg
     6841 tgtaagaaca tcagtggccg ttacgttaac ggtcactgcg gcatgcgccg atcgccctcg
     6901 ttcgaacctc tcgtggttca gcccggactg ctcgcggctg ataacgtccc ttaagccagg
     6961 gaatggcctg acctgattgc cggtctcgca gattttagct gttttctcag gattctcgga
     7021 ttgggctgcc cggtacagta tccccggagg gacccggctg ttttacctgc tgccggtagg
     7081 caggtgcgcg tcagagtgcg cctcctcatg gtttgactaa atactagcca ggtttttcgt
     7141 gcgtgtgaat gttgtttttt aaatgttgcg cctgactccg ctcaatagcg acacccgcta
     7201 tgtacaggcg catttatgcg tcgtacatag cgggtgtcgc aggtgctgat ttggcggaat
     7261 aaaaaacacc gggactgacc gccccggacc ccgccaacgg tgaccggaca gacaaaaaat
     7321 ttgcctgccc ggtcaccgtt tcacatcatg gcgaggaggc gggatctgaa acggtgtgat
     7381 gaaagtgcag atgaggtcag acgatgcagg tcggtgagtc cgtggtgaac gcgatagtgg
     7441 aataccacgc acagggcagt gaggaggcgc atgcagagga aaacaggatg ttcctgcgtt
     7501 atctgcgcga gttaacggga acacagcgtg tggcggttga ggtggccagg aaatattttt
     7561 ctccggtgag gggctgatat gagctatctt tttcttgtcg ccggcaccgc accaggtgcg
     7621 acggtccttt tacagtgttg tcctcatttg acgggcctgg atggcccgtt cttttatgct
     7681 tttaacgtcc ggggcccttt tctcggccga aatcaccggc tatgcggtgg tgtgcatagc
     7741 catgcatatg cgtaaaataa atcaggcgcg tttctggcgg ttttcggtgt acttgccacc
     7801 accttttgcc cgttaaccac cagaaacgcc ctgaggccgt tttcgcggtg cgcgtaagaa
     7861 gggattatgg taaattctat atgtacttaa ttgcgtacat gttctatgct ggtactacaa
     7921 ctgaagggag ccaataagat gcatgcaatc acttttacgg atgcccgtaa acattttgcc
     7981 gataccatga agcgtgttac cgatgatgct gagccagtac gagtaatgcg gcgagatgca
     8041 cctgatatcg tcatgatcga tgctggagag tatgaggcac tcatagaaac agtgtatctg
     8101 ttcagcaatc ctgcgaatgc tgctcatatc aatgagtcaa tgaaacaggc ggagcgcggc
     8161 gaatttgtcg aagtggaata ttaagttcac gaagaatggc gctgatgata tcaattactg
     8221 gcgtggtact gacaaaaaga agtttgaacg tatccggcag ctacttcaca gcatcgagtc
     8281 tgatccgtta actggtatag ggaagcctga gcgattacgc catcacaaag atcctgcgct
     8341 ctattctcgg cgtatcgatc agagccatag gcttgtatat tcaatccaaa acggagaaat
     8401 tatcatctac gcagcgcgtt atcactacgg cgataaatag aaataaaaat attttacatt
     8461 tagctatcgg gtttatattt ttttctaaaa ggatttttcc aactggtgat tttaaaatga
     8521 gaactcatta cgataatttg aaggtgtctc aggatgcacc tgtcgaggtg attcaggctg
     8581 cttatagaac ccttgcaaaa aaataccatc ctgatttggg taatgactcc aacttactga
     8641 tagtgtttta tgttcagata atgcccgatg accttgtcat gcagctccac cgattttgag
     8701 aacgacagtg acttccgtcc cagccttgcc agatgttgtc tcagattcag gttatgtcgc
     8761 tcaatgcgct gagtgtaacg cttgctgata acgtgcagct ttcccttcag gcgtgattc
//
