LOCUS       NZ_CP080065             1718 bp    DNA     circular CON 14-JUN-2024
DEFINITION  Escherichia coli strain 18SZHZ655Rt plasmid p655Rt_10, complete
            sequence.
ACCESSION   NZ_CP080065
VERSION     NZ_CP080065.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20339660
            Assembly: GCF_023516035.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Liu,B., Liu,W. and Fu,Y.
  TITLE     Genetic environment and risk factors analysis of mobile tigecycline
            resistance genes tet(X) variants in healthy human carriages: a
            cross-sectional and case-control study
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Liu,B., Liu,W. and Fu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2021) Key Laboratory of Genetics & Molecular
            Medicine of Shenzhen, Shenzhen Center for Disease Control and
            Prevention, Longyuan Road 8, Shenzhen 518055, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023516035.1-RS_2024_06_14
            Annotation Date                   :: 06/14/2024 05:58:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,979
            CDSs (total)                      :: 4,858
            Genes (coding)                    :: 4,716
            CDSs (with protein)               :: 4,716
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 66 of 142
            Pseudo Genes (incomplete)         :: 91 of 142
            Pseudo Genes (internal stop)      :: 26 of 142
            Pseudo Genes (multiple problems)  :: 35 of 142
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080065.1.
            Bacteria and source DNA available from Shenzhen Center for Disease
            Control and Prevention.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="18SZHZ655Rt"
                     /isolation_source="Human feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p655Rt_10"
                     /geo_loc_name="China:Shenzhen"
                     /altitude="120 m"
                     /collection_date="2018-12-08"
                     /collected_by="Bang Liu"
     gene            complement(519..923)
                     /locus_tag="KZO13_RS24780"
                     /old_locus_tag="KZO13_24755"
     CDS             complement(519..923)
                     /locus_tag="KZO13_RS24780"
                     /old_locus_tag="KZO13_24755"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061604172.1"
                     /translation="MKILAAMEIADCDFEHMDMDVLVGALLKLKLWDSHHHNLNMQGKR
                     SFLDDLRADGIEAIKKYEAEIESENESFAEERLILRIYQNELGLWLGRLFCGEAEIGQC
                     GVYGSADEVRTAARQSGILCDSIEIFQKDA"
                     /name="hypothetical protein"
     gene            complement(1137..1373)
                     /locus_tag="KZO13_RS24785"
                     /old_locus_tag="KZO13_24760"
     CDS             complement(1137..1373)
                     /locus_tag="KZO13_RS24785"
                     /old_locus_tag="KZO13_24760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016230891.1"
                     /translation="MTRLTNLTPAEKKFIDDAIAAAERAAGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     Typing          complement(1531..1615)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.512"
                     /coverage="100.0"
                     /evalue="3.06e-32"
ORIGIN
        1 ctacgctccg gacgtgagac tgcggcgggc actacaactt ttttcacata catgaagtga
       61 ggtgtgacgg ggatcttgtt gaaagatcag gctacagatg tacaaaagcc ccgtgacaga
      121 atcacagggc ttcggtactc taattcttcg cagatacctg ccaggatcag acgtagcaat
      181 cctatcctag cagggctggc tagccttgtc actaccagct taataccatt caacgacatt
      241 aaaatcatat ctgcaccctt gaaactcagg tctacgcccc ggactccggc aggataaaca
      301 ggcaaacggt ggaaacgtaa cttaaccgcg aaagagttaa gctctacccc gtctcgctgt
      361 acgataacag gcacctgata aagtgagatt caaggcagga gttgcggtga aatgcgattg
      421 cccagtctgg cacaacaccc acacaatcag aacaacatag aacgacctga cgctggtcgc
      481 tccgctcccc gttttctggt gtacccacca cgggggcatt aggcatcctt ttgaaaaatt
      541 tcgatgctat cgcacaaaat accgctttga cgcgcagctg ttcgaacctc atcagccgat
      601 ccataaacac cacactgacc gatctcagcc tcaccacaaa agagcctccc taaccataag
      661 ccaagctcgt tctgatagat cctcagtatt aatcgctcct ccgcaaagga ctcattctca
      721 gattcaattt cagcctcgta tttcttaata gcctcaatac catccgctct taaatcatcg
      781 agaaagctcc tcttaccttg catgttcaag ttgtgatggt ggctatccca caattttaat
      841 ttcagtaaag cccctactaa gacatccata tccatatgtt caaaatcaca atcagctatt
      901 tccattgcag ccaaaatctt catacaccct ccgatttacc ataacatccg ttatcagcac
      961 cccctgacag gccctcagcg caattctgac gcatcagagc cgtttccggc acatttcctc
     1021 acccacatcc cggaaaaacg cccctgatga tttctgcgcg actgcatagc tatgcacgcg
     1081 ggtgaaccgc aggtgatttc gcagcatcag caccactaaa ccgccaaaat ccgcaatcag
     1141 cggcgcggcg cgcgcggacg tgaccaggca aaatcggact gctgccgctc atcctcacgt
     1201 aatgcacgct ggcggtcggc gtaacgctgc gactcaatct gcgcacgcgc tctgttcagg
     1261 acgatgtgcc ggttaggctg attgagcttc ttcccggccg cacgttcggc agcggctata
     1321 gcgtcgtcga taaacttttt ctcagcgggg gtcaggtttg tcagtctcgt catgtttatc
     1381 tccgtatgag aatagcccct gcagtctgcc agacacagta ggaacgatga agcgtaagcg
     1441 acggtgaaag gcagtcagaa acggtgaacg ggagcggatt ttaatccggt gccgtgaggc
     1501 gttggacaag ccgcaggcgc gtcagtggat ttagcgggtt tcggggcgca gccctgaacc
     1561 agtcacgtag cgctagcgga gtgtatactg gcttaatatg ttggcatagg tgcggttatc
     1621 aggaaagtgc gtcatgtggc aggtgtgata tgtggctcag aagcgtccgc aataccatgc
     1681 ccgaatagtg aaatgatgcc tcctcgctca ctcggtcg
//
