LOCUS       NZ_CP080064             1943 bp    DNA     circular CON 14-JUN-2024
DEFINITION  Escherichia coli strain 18SZHZ655Rt plasmid p655Rt_9, complete
            sequence.
ACCESSION   NZ_CP080064
VERSION     NZ_CP080064.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20339660
            Assembly: GCF_023516035.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Liu,B., Liu,W. and Fu,Y.
  TITLE     Genetic environment and risk factors analysis of mobile tigecycline
            resistance genes tet(X) variants in healthy human carriages: a
            cross-sectional and case-control study
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Liu,B., Liu,W. and Fu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2021) Key Laboratory of Genetics & Molecular
            Medicine of Shenzhen, Shenzhen Center for Disease Control and
            Prevention, Longyuan Road 8, Shenzhen 518055, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023516035.1-RS_2024_06_14
            Annotation Date                   :: 06/14/2024 05:58:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,979
            CDSs (total)                      :: 4,858
            Genes (coding)                    :: 4,716
            CDSs (with protein)               :: 4,716
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 66 of 142
            Pseudo Genes (incomplete)         :: 91 of 142
            Pseudo Genes (internal stop)      :: 26 of 142
            Pseudo Genes (multiple problems)  :: 35 of 142
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080064.1.
            Bacteria and source DNA available from Shenzhen Center for Disease
            Control and Prevention.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="18SZHZ655Rt"
                     /isolation_source="Human feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p655Rt_9"
                     /geo_loc_name="China:Shenzhen"
                     /altitude="120 m"
                     /collection_date="2018-12-08"
                     /collected_by="Bang Liu"
     gene            complement(225..338)
                     /locus_tag="KZO13_RS24935"
     CDS             complement(225..338)
                     /locus_tag="KZO13_RS24935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001517211.1"
                     /translation="MHEPPVQSDCCACSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     Typing          complement(226..707)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.144"
                     /coverage="92.0"
                     /evalue="7.55e-176"
ORIGIN
        1 cactgactcg ttagatttca tgttgctact tgagattatt taattcagcg ggtagtcttt
       61 cgcttgaaaa agaaaaaacc gccctgcaag gcggtttttc gaaggttctc tgagctacca
      121 actctttgaa ccgaggtaac tggcttggag gagcacagtc accaaaactt gtcctttcag
      181 tttagcctta accggcgcat aacttcaaga ctaactcctc taaatcaatt accagtggct
      241 gctgccagtg gcgcttttgc atgtctttcc gggttggact caagtcgata gttaccggaa
      301 caggcgcagc agtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact
      361 gccttcccgg aaccgagtgt caggcgtgga atgagataaa cgcggccata acagcggaat
      421 gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagctac cagggggaaa
      481 cgcctgggat ctttatagtc ctgtcgggtt tcgccgccac tgatttgagc gtcagatttt
      541 gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc aacgccgctt ccttttctct
      601 tcctttccca atttgattgc tactagccta ctcctttact tctgagccgg taacgcccgc
      661 cgcagtctca cgtccggagc gtagcgaccg agtgagcgag gaggcgtaat ttcatcgact
      721 cctgcatttt tcacttacgc cctacagcta catttttcac ctgccagggg tcagtttgga
      781 tatagagaat tattgtacgg taagcctgtt ttttgaacag acttacctat agcaataggg
      841 tgtactcccg cgctatacgg cattttcaga ggtttgacct taatcggata acctaaatgc
      901 gatactgcaa gagcgacaac ttaaaagcga tagccactga cgcaaaatca accccttacg
      961 aaccaagatt ggttaaataa tgcgcttaac gtacaaaaat ttccgatctc caaactgacc
     1021 ccttcttcgc ctcttatgtt tctcgaatga atcgatgggg acaggaaatg ttttgtggcc
     1081 accgccgctt cgccggcacc acaaaactgt cggacaagat gtgtttttgg agtaccgccg
     1141 gcaccaaaaa catttcatca ggcgcagcct gataccctga atttcgtacg tacgaaattt
     1201 tgcttttgtt cagatcgagg gcaggattcg caaagtgctg gcgtttgtgc tcacatcctt
     1261 tagacgttgc gatgcaggat agggcagacg ccgcaaaatg acgtcatttg cggtatctgc
     1321 cccgaaaaca gaatgttttc tcctgagcct gcagctggct ctcagaaccc ctaccgcctc
     1381 gcaaagctcg ttgctcaacg cctcacgacc gcacagtaaa ctgtgcaccc gttcaccgtt
     1441 atcgatgggg ttcagcgagc cttttgagca gttcgcagga ttttagccgc taaaattccg
     1501 gccaggcatg ttcgtgcata gccatgcaga tgcgcagaaa tcatcaggcg cgtttttcag
     1561 gggttggtgc tgggaaaggt gccggaaacg gcccgttcgc ctcaaaatgg ctctgaggcg
     1621 ttttcagcgg tgcgggcaag ggatgttatg gtaaatatcg tttatgttga ttaacaaatt
     1681 aaaagataag attattccta cttctctatt ttctggggct tttatgaaaa aggtaattct
     1741 ctcattggtt tttctgtcat cagtaattct tgctggctgc tcaagccaag caggtccatt
     1801 tgtgaccaac attagcagtg atggtagagg tgggcttgtc gttgagaaat gtatggttcg
     1861 ccttgataga atgattaata ccgttgaatc atctagctgc actaattcaa gtattactct
     1921 gtcctcacca tcttctagct cga
//
