LOCUS       NZ_CP080062             4593 bp    DNA     circular CON 14-JUN-2024
DEFINITION  Escherichia coli strain 18SZHZ655Rt plasmid p655Rt_7, complete
            sequence.
ACCESSION   NZ_CP080062
VERSION     NZ_CP080062.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20339660
            Assembly: GCF_023516035.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Liu,B., Liu,W. and Fu,Y.
  TITLE     Genetic environment and risk factors analysis of mobile tigecycline
            resistance genes tet(X) variants in healthy human carriages: a
            cross-sectional and case-control study
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Liu,B., Liu,W. and Fu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2021) Key Laboratory of Genetics & Molecular
            Medicine of Shenzhen, Shenzhen Center for Disease Control and
            Prevention, Longyuan Road 8, Shenzhen 518055, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023516035.1-RS_2024_06_14
            Annotation Date                   :: 06/14/2024 05:58:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,979
            CDSs (total)                      :: 4,858
            Genes (coding)                    :: 4,716
            CDSs (with protein)               :: 4,716
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 66 of 142
            Pseudo Genes (incomplete)         :: 91 of 142
            Pseudo Genes (internal stop)      :: 26 of 142
            Pseudo Genes (multiple problems)  :: 35 of 142
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080062.1.
            Bacteria and source DNA available from Shenzhen Center for Disease
            Control and Prevention.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="18SZHZ655Rt"
                     /isolation_source="Human feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p655Rt_7"
                     /geo_loc_name="China:Shenzhen"
                     /altitude="120 m"
                     /collection_date="2018-12-08"
                     /collected_by="Bang Liu"
     gene            51..611
                     /locus_tag="KZO13_RS24740"
                     /old_locus_tag="KZO13_24710"
     CDS             51..611
                     /locus_tag="KZO13_RS24740"
                     /old_locus_tag="KZO13_24710"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067488.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKQQLDTLSTTVSTQLSTTEAELSRQQKNLLWQVIKGRVLFPALTA
                     LSVTGGIFLGCWGLIQWQESRIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            618..851
                     /locus_tag="KZO13_RS24745"
                     /old_locus_tag="KZO13_24715"
     CDS             618..851
                     /locus_tag="KZO13_RS24745"
                     /old_locus_tag="KZO13_24715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137388.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHAVTQQNNA
                     ILNERVVNLSQQVQLLAGQVDRLSRLFITNNR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1539..2378)
                     /locus_tag="KZO13_RS24750"
                     /old_locus_tag="KZO13_24720"
     CDS             complement(1539..2378)
                     /locus_tag="KZO13_RS24750"
                     /old_locus_tag="KZO13_24720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001354646.1"
                     /translation="MLTLFIFFVLLIAACFFCFAPPRRGYDRNEIIPYKIKLSINKYRL
                     YIYSSGKVRQYLLFLVILSLYYSIAEPFKSELIKNISYSLMAAFIFDTGLNFSKENITK
                     GVISTRWHNDLYSSFERMKAINKIYYPSNKEINTEGLSKAITSSLFNDDANSFAKRDFR
                     LMWDLSSEKYLSYKEIIIRKGDKLDAVCLRFINDDYKFLVNFNRDEEVFKYFPSIMQPS
                     LKTYRALSRLVNSIKDPSRFKFTTESLEMELLEYLELRNELFNDIEEVMGSYAQRAP"
                     /name="hypothetical protein"
     gene            complement(2570..2719)
                     /locus_tag="KZO13_RS24930"
     CDS             complement(2570..2719)
                     /locus_tag="KZO13_RS24930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032194431.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPERHVKTPLAAAIGNRLEELVLK
                     LCAG"
                     /name="hypothetical protein"
     gene            complement(3389..3580)
                     /locus_tag="KZO13_RS24755"
                     /old_locus_tag="KZO13_24725"
     CDS             complement(3389..3580)
                     /locus_tag="KZO13_RS24755"
                     /old_locus_tag="KZO13_24725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001677262.1"
                     /translation="MTKQEKTAINMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3643..3966
                     /locus_tag="KZO13_RS24760"
                     /old_locus_tag="KZO13_24730"
     CDS             3643..3966
                     /locus_tag="KZO13_RS24760"
                     /old_locus_tag="KZO13_24730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001677263.1"
                     /translation="MLTIRVSDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     SPPLLRHLAAIGNNLNQTARKVNSGHWSSIDRVHVVAALMAIEGELRQLRQAVREQGGR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          3217..3301
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          complement(2648..3206)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.761"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3..949
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3957..4591
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="2.65e-137"
ORIGIN
        1 tattgccgac ccggaggggg ggcggaatct caggctaaag ggggcgctgt atgagcaatc
       61 ttttacaaat gggaacggac ttcgagaaga aactgaaaga gcgagccgct tctaccgaga
      121 acatgctgaa cagcgagttc aggaagctag aagagtctgt agacaaggca ctgagcttaa
      181 acaggcagaa aatcagagac gctatcagcg aacatacaac atccgtgaaa cagcaactgg
      241 atacgctgag caccaccgtg agcacgcagc tcagcaccac agaggccgaa ctgtcccggc
      301 agcagaaaaa cctgctctgg caggtgatca aagggcgggt actgtttccg gcgctgaccg
      361 cgctcagcgt cacgggcggc attttcctgg ggtgctgggg gctgattcag tggcaggaaa
      421 gcaggattgc gaaaaatatc ttgaccatca gagagcagga aaacacgctg gcgaaactcg
      481 aggcaaaaac ctggggggtg acgttcgtca atggcgagaa cgggaaattt ctggtgctgc
      541 cggacggggt gaaaggggaa aacacctgga cggtgggcga caagaacgcc gtgaggctgg
      601 tcagggagta aacgagtatg acggaactgg aagggcattt gctgaacgcc ttagagcatt
      661 tgcaacagga ctatatgcgg cggctgaacg agtgggagag cgccttcgca gaattgcaga
      721 agatgcacgc ggttacgcaa cagaacaacg cgatactgaa cgagcgtgtc gtgaacttga
      781 gtcagcaggt gcagctatta gccggacaag tcgaccgctt gagtcggtta tttatcacga
      841 acaacagata agggatgaac aggagcgtca ggcgcaggag cttgcccggc gctggcatga
      901 agagcaggaa cccgaagcgc ggatctggcg tggcccgtca atggggatgt gaccggggta
      961 ccccctgaaa tcgagccagt ttgtggcacg ccctacccgg atggcgccaa tgttaatgta
     1021 cgtacattag cattaacaat atccaaacct ccccattttg ccccgttcga cggctggaac
     1081 gtgcgaaact gatcgtcatt cagtttcgcg cataactatg catgaggtta aaatttacca
     1141 ggcgcgatcg cggcagtttt tcgggtggtt tgttgctgtt tttacctgtc tgctgccgtg
     1201 atcgcgctga acgcgtttaa gcggtacgcg caatgcgacg ttatggtaaa tacggagtat
     1261 tttaattgcg ggcctttgtt gtatatctct gtcgtttgtc cccgggggga agcattactg
     1321 ctaacaagtc ccgtgagaaa actgcgcaat cgtattgcgc accatgaacc tattctcaac
     1381 cgcaatctgg aggatgattt tgctacgata aagagaatta tcgcgtatcg gtgtcagcat
     1441 tcattggagt ggatgctgaa aaaccaggtg ttactccctc tacttacact taaacctctc
     1501 taaaaatata agtaagtact atcgagggct atcatgaact atggtgccct ctgagcatag
     1561 ctacccatta cttcttctat gtcattgaat aattcatttc ttaactctaa gtattctagt
     1621 aactccattt ctaatgactc tgtcgtgaac ttaaatctag aaggatcttt gattgagttt
     1681 actagtcttg ataacgctct atatgttttg agtgaaggtt gcattataga tggaaagtat
     1741 ttgaaaacct cttcatctct gttgaaatta actaaaaact tgtagtcatc atttataaat
     1801 ctcaagcata cagcgtcaag cttatcgcct tttcttatta ttatttcttt ataggaaaga
     1861 tatttttcag aagataagtc ccacatcaag cgaaaatctc tctttgcgaa cgagttcgcg
     1921 tcatcattga aaagagatga tgtgatggct ttggatagac cttctgtgtt tatctctttg
     1981 ttggatgggt agtaaatttt attaattgct ttcattctct caaatgatga atataggtca
     2041 ttatgccacc ttgttgaaat cactccttta gttatattct ccttgctaaa attaagccct
     2101 gtatcaaaaa taaatgcagc catcagtgaa tatgatatgt tttttattaa ctcagactta
     2161 aatggttccg ctatggagta gtacagtgat aaaataacta aaaataaaag gtattgtctt
     2221 acctttcccg atgagtatat gtaaaggcga tatttattta ttgatagttt tattttgtat
     2281 ggaatgatct catttctgtc atatcctctt cgtggtggag caaaacaaaa gaaacatgca
     2341 gctattaata aaacaaaaaa gatgaacagt gtaagcaagt tcggctcctg tgtatgctca
     2401 ctatgcatga ttgtcttgag atctaacgat cctgaaggta gtcttgtccg taaacgaaaa
     2461 aaccgcccag caaggcggct tttcgaaggt tctcagagca gcaactcttt gaaccaaggt
     2521 aactggcttg gaggagcaca gccaccaaat ctgtcctttc agtttagcct taaccggcgc
     2581 ataacttcaa gactaactcc tctaaacggt taccaatggc tgctgccagt ggcgttttta
     2641 cgtgtctttc cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     2701 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gggccgagat
     2761 accaacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     2821 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     2881 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcat ctgtttttgt
     2941 gatgctcgtc aggggggcgg agcctatgga aaaacgccag cggcgcggcc ttcttcctgt
     3001 tcctggcctt ttgctcacat gtttcttttg gcgttatccc ctgattctgt ggataaccgc
     3061 atcaccgtct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgtagcgag
     3121 tcagtgagcg aggaagcgga agagcgcctg atgcggcatt ttctccttac gcatctgtgc
     3181 ggtatttcac accgcatatg gtgcactttt cttataatcc gcactgatgc cacatagtta
     3241 agccagtata cactccgcta tcgctacgtg actgggtcag ggctgcgccc cgacacccgc
     3301 taaaacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct cacaggcaag
     3361 ctgtgaacgt ctccgggagc tgcatgtttc agttgctttc accgtcatcg ccgaagcgtg
     3421 cgaggcaact acggtagagt tcgtcggcgt ggtcgtgaag tgattcgcag atgtctgcct
     3481 gttcgtccgc gtccagctcg ttgagttttt ccagcagggt cagcgtctgg cttctgataa
     3541 agcgggccat gttaatggct gttttttcct gtttggtcat ggtcttaagg ctccttaata
     3601 ggggattttt tagggggaat gataccgatg aaacgcgaga agatgctcac aatacgggtt
     3661 agcgatgagg agcacgcccg gttactggaa cgctgcgagg gtaaacgact ggcagaatgg
     3721 atgcggcggg tctgtctggg ggagccggtt gcgcgcacag gaaaactgcc gacactatcg
     3781 ccgccattgc tgcggcatct ggctgccatc gggaacaatc tgaaccagac agcccggaag
     3841 gtgaacagcg gccactggtc ttcgattgac cgggttcatg tggtggccgc tctgatggcc
     3901 atcgagggtg aattgcgcca gttgcggcag gctgtcaggg aacagggggg acgggatgat
     3961 agttaaattt catgccaggg ggaaaggcgg tggaagtggc ccggtcgatt acctgctggg
     4021 gcgggagcgt aaccgcgaag gcgcaacggt gctccggggt aacccggaag aaatccggga
     4081 actcattgat gccacgccat tttcgaagaa atacacgtca ggcgttctgt cgttcgcgga
     4141 gaaggagtta ccgccgggtg aacgcgaaag agtgatgacg agctttgagc gtgttcttat
     4201 gcccggtctc gaaaaagacc agtacagcat tctctgggtg gaacaccagg ataaggggcg
     4261 gctcgaactg aattttgtca ttccgaacat ggaactacag agcggtaagc gcctccagcc
     4321 gtactacgac cgtgcggaca ggccgaggat aaatgcctgg cagacgctgg ttaatcatca
     4381 ttacgggctg cgtgacccga acgcaccgga gaacagacgg acgcttgtca caccgaataa
     4441 tctgccaaaa gcgaaacagg aggccgcaga agcgattaca cggggtcttg aggcgcttta
     4501 ccgtgccgga gcaataaaaa cgcgtcagga tgtcactgag gcgctcacag cggcaggttt
     4561 tgaggtggtg aggacaacca gaagcagtat cag
//
