LOCUS       NZ_CP080060             6647 bp    DNA     circular CON 14-JUN-2024
DEFINITION  Escherichia coli strain 18SZHZ655Rt plasmid p655Rt_5, complete
            sequence.
ACCESSION   NZ_CP080060
VERSION     NZ_CP080060.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20339660
            Assembly: GCF_023516035.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Liu,B., Liu,W. and Fu,Y.
  TITLE     Genetic environment and risk factors analysis of mobile tigecycline
            resistance genes tet(X) variants in healthy human carriages: a
            cross-sectional and case-control study
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Liu,B., Liu,W. and Fu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2021) Key Laboratory of Genetics & Molecular
            Medicine of Shenzhen, Shenzhen Center for Disease Control and
            Prevention, Longyuan Road 8, Shenzhen 518055, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023516035.1-RS_2024_06_14
            Annotation Date                   :: 06/14/2024 05:58:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,979
            CDSs (total)                      :: 4,858
            Genes (coding)                    :: 4,716
            CDSs (with protein)               :: 4,716
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 66 of 142
            Pseudo Genes (incomplete)         :: 91 of 142
            Pseudo Genes (internal stop)      :: 26 of 142
            Pseudo Genes (multiple problems)  :: 35 of 142
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080060.1.
            Bacteria and source DNA available from Shenzhen Center for Disease
            Control and Prevention.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="18SZHZ655Rt"
                     /isolation_source="Human feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p655Rt_5"
                     /geo_loc_name="China:Shenzhen"
                     /altitude="120 m"
                     /collection_date="2018-12-08"
                     /collected_by="Bang Liu"
     gene            join(6471..6647,1..57)
                     /locus_tag="KZO13_RS24675"
                     /old_locus_tag="KZO13_24645"
     CDS             join(6471..6647,1..57)
                     /locus_tag="KZO13_RS24675"
                     /old_locus_tag="KZO13_24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(375..794)
                     /locus_tag="KZO13_RS24680"
                     /old_locus_tag="KZO13_24650"
     CDS             complement(375..794)
                     /locus_tag="KZO13_RS24680"
                     /old_locus_tag="KZO13_24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(875..1312)
                     /locus_tag="KZO13_RS24685"
                     /old_locus_tag="KZO13_24655"
     CDS             complement(875..1312)
                     /locus_tag="KZO13_RS24685"
                     /old_locus_tag="KZO13_24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            1572..3140
                     /gene="cea"
                     /locus_tag="KZO13_RS24690"
                     /old_locus_tag="KZO13_24660"
     CDS             1572..3140
                     /gene="cea"
                     /locus_tag="KZO13_RS24690"
                     /old_locus_tag="KZO13_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(3137..3478)
                     /locus_tag="KZO13_RS24695"
                     /old_locus_tag="KZO13_24665"
     CDS             complement(3137..3478)
                     /locus_tag="KZO13_RS24695"
                     /old_locus_tag="KZO13_24665"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_000058760.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            3526..3663
                     /locus_tag="KZO13_RS24700"
                     /old_locus_tag="KZO13_24670"
     CDS             3526..3663
                     /locus_tag="KZO13_RS24700"
                     /old_locus_tag="KZO13_24670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(3862..4020)
                     /locus_tag="KZO13_RS24925"
     CDS             complement(3862..4020)
                     /locus_tag="KZO13_RS24925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(4690..4881)
                     /locus_tag="KZO13_RS24705"
                     /old_locus_tag="KZO13_24675"
     CDS             complement(4690..4881)
                     /locus_tag="KZO13_RS24705"
                     /old_locus_tag="KZO13_24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4945..5268
                     /locus_tag="KZO13_RS24710"
                     /old_locus_tag="KZO13_24680"
     CDS             4945..5268
                     /locus_tag="KZO13_RS24710"
                     /old_locus_tag="KZO13_24680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5477..>5908
                     /locus_tag="KZO13_RS24715"
                     /pseudo=""
     CDS             5477..>5908
                     /locus_tag="KZO13_RS24715"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5946..6464
                     /locus_tag="KZO13_RS24720"
                     /old_locus_tag="KZO13_24690"
     CDS             5946..6464
                     /locus_tag="KZO13_RS24720"
                     /old_locus_tag="KZO13_24690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_016230734.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     Typing          4518..4605
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(3949..4504)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.699"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5259..6646
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.568"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 cacttgagcg agcagacaga acgcttgagc cagttataca gcgagaactg gagataagag
       61 aggaacggct gatacaggag cgcgaacatg tgttatccct ggaacgggag cgtcagccgg
      121 aaatacagga acgcacgctg gatggccctt cgctgggatg gtgaaaccat gaaaaatggc
      181 agcttcagtg gattaagtgg gggtaatgtg gcctgtaccc tctggttgca taggtattca
      241 tacggttaaa atttatcagg cgcgatcgcg gcagtttttc gggtggtttg ttgccatttt
      301 tacctgtctg ctgccgtgat cgcgctgaac gcgttttagc ggtgcgtaca attaagggat
      361 tatggtaaat ccacttactg tctgccctcg tagccatcga gataaaccgc agaaatcgtg
      421 tcagccagca gcctggattt tttcggggta aggtctttgc tgcggtaatc agtacccgca
      481 aattgagttg tgccccggag tgagtttaaa aattcctggc tgtgaaactg ctgaatgcgt
      541 tgttgcacat cactccgtga ttttcctgtc gctttatctt tagctcccat atcccagaac
      601 tgccgaaaaa aaggcaccat cattcgtgtt gtgtcggctt tttgggtgga aaagttggga
      661 tcaaaaccgt catcagaagc atatacgtaa tgccgggtat gttgttccac gctgcccttt
      721 ggggtacagg ctgtgagagc tactgttgct gttatcatgg cgataagaca aagtttgttt
      781 ccggtacgtc tcatgagtgt ctccctacct taaagtattt attctcggtg agttattatc
      841 cgaagactat tttatcatca gtctctgaca gcgttcagcg accgaacaac ctggaccaga
      901 atccccgttt cttttccagt tccagtgcct gcctgagctg ggctatctca ttttgtagct
      961 gttcccgttc tgcttcctgg cgtctgcgat ccatatcctg tgcctgttta tcctcaagca
     1021 tcagcgtcag gcattgtttc agctcattca gctcccggag aatgcgttct gtctgctgat
     1081 catgtggatt ttctgcatgt ccctcactgt gcctttctgg tgtctcattc tgctttaatt
     1141 cgccgtatgc ccggatcagt tcactggttt caaactcccg tcgtccgtcc ctgccggtac
     1201 ggtaactgac aaggcctgcg cgcatatcac ggtaaaactg tgaacgcgat ctgcctgtca
     1261 tttttagtgc gtcccggagt gtgtgccatg ccataaagtg acagtgtccc atagatgtct
     1321 catctcatag tttcagtaaa acataatgag gtctgagaac ggtaatgttt gtgctggttt
     1381 ttgtggcatc gggcgagaat agcgcgtggt gtgaaagact gtttttttga tcgttttcac
     1441 aaaaatggaa gtccacagtc ttgacaggga aaatgcagcg gcgtagcttt tatgctgtat
     1501 ataaaaccag tggttatatg tacagtattt atttttaact tattgtttta aaagtcaaag
     1561 aggattttat aatggaaacc gcggtagcgt actataaaga tggtgttcct tatgatgata
     1621 agggacaggt aattattact cttttgaatg gtactcctga cgggagtggc tctggcggcg
     1681 gaggtggaaa aggaggcagt aaaagtgaaa gttctgcagc tattcatgca actgctaaat
     1741 ggtctactgc tcaattaaag aaaacacagg cagagcaggc tgcccgggca aaagctgcag
     1801 cggaagcaca ggcgaaagca aaggcaaaca gggatgcgct gactcagcgc ctgaaggata
     1861 tcgtgaatga ggctcttcgt cacaatgcct cacgtacgcc ttcagcaaca gagcttgctc
     1921 atgctaataa tgcagctatg caggcggaag cagagcgttt gcgccttgcg aaagcagaag
     1981 aaaaagcccg taaagaagcg gaagcagcag aaaaggcttt tcaggaagca gaacaacgac
     2041 gtaaagagat tgaacgggag aaggctgaaa cagaacgcca gttgaaactg gctgaagctg
     2101 aagagaaacg actggctgca ttgagtgaag aagctaaagc tgttgagatc gcccaaaaaa
     2161 aactttctgc tgcacaatct gaagtggtga aaatggatgg agagattaag actctcaatt
     2221 ctcgtttaag ctccagtatc catgcccgtg atgcagaaat gaaaacgctc gctggaaaac
     2281 gaaatgaact ggctcaggca tccgctaaat ataaagaact ggatgagctg gtcaaaaaac
     2341 tatcaccaag agccaatgat ccgcttcaga accgtccttt ttttgaagca accagacgac
     2401 gggttggggc cggtaagatt agagaagaaa aacaaaaaca ggtaacagca tcagaaacac
     2461 gtattaaccg gataaatgct gatataactc agatccagaa ggctatttct caggtcagta
     2521 ataatcgtaa tgccggtatc gctcgtgttc atgaagctga agaaaatttg aaaaaagcac
     2581 agaataatct ccttaattca cagattaagg atgctgttga tgcaacagtt agcttttatc
     2641 aaacgctgac tgaaaaatat ggtgaaaaat attcgaaaat ggcacaggaa cttgctgata
     2701 agtctaaagg taagaaaatc ggcaatgtga atgaagctct cgctgctttt gaaaaataca
     2761 aggatgtttt aaataagaaa ttcagcaaag ccgatcgtga tgctattttt aatgcgttgg
     2821 catcggtgaa gtatgatgac tgggctaaac atttagatca gtttgccaag tacttgaaga
     2881 ttacggggca tgtttctttt ggatatgatg tggtatctga tatcctaaaa attaaggata
     2941 caggtgactg gaagccacta tttcttacat tagagaagaa agctgcagat gcaggggtga
     3001 gttatgttgt tgctttactt tttagcttgc ttgctggaac tacattaggt atttggggta
     3061 ttgctattgt tacaggaatt ctatgctcct atattgataa gaataaactt aatactataa
     3121 atgaggtgtt agggatttaa ttattcttta ttgatataaa aagtcctagc aatccaaatg
     3181 ggattgctag gaccaaacaa agtagattat atagcataaa taggtttaat tttgctacgg
     3241 gggcgttatt taggtttttt cttctttcga aaaaatcttt ctttatgaag ttaaaagcta
     3301 tgtattcaat agcatatttt gaatatggac atagaatagt gcttatcact attgcatata
     3361 gcatcttatc tgacacaagg aaataatacc cttcgctgtt ttttgttata aggtatatat
     3421 atataagtgt gcagtacagg ccaaataaaa tattttttat gtagtatctt aagctcataa
     3481 attaaacctc gccatatatt cttttcattt tataaggatc gagttatgag gaaaagattt
     3541 tttgtgggaa tattcgcgat aaacctcctt gttggatgtc aggctaacta tatacgtgat
     3601 gttcagggag ggaccatcgc accatcctcc tcttctaaac tgacggggat cgcggttcag
     3661 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3721 aaacaaaaaa accaccgcta ccaacggtgg tttgtttgcc ggatcaagag ctaccaactc
     3781 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     3841 agccgtagtc gggccactac ttcaagaact ctgtagcacc gtttgtgcca tcatcgctct
     3901 gctaatccgg ttaccagtgg ctgctgccag tggcgttaag gcgtgcctta ccgggttgga
     3961 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     4021 acagcccagc ttggagcgaa cgacctacac cgaactgaga taccaacagc gtgagctatg
     4081 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4141 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4201 tgtcgggttt cgccacctct gacttgagcg tctatttttg tgatgctcgt caggggggcg
     4261 gagcctatgg aaaaacgcct gctacgtggc cttcttcctg ttcctggtct tttgctcaca
     4321 tgttctttcc ggccttatcc cctgattctg tggataactg tgttaccgtt tttgtgtgag
     4381 tcagtaccgc tcgccgcagt cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg
     4441 aaaagcgcct ggacgtgcat tttctcctta cgcatctgtg cggcatttca cacccggcat
     4501 ggcgtacttt tcatacaatc cgcactgatg ccgcatggtt aagccagtat acactccgct
     4561 atcgctacgt gactgggtca gggctgcgcc ccgacacccg ctaaaacctg ctgacgcgcc
     4621 ctgacgggct tgtcagctcc cggcatccgc tcacagacaa gctgtgaccg tctccgggag
     4681 ctgcatgtgt cagaggtttt caccgtcatc cccgaaacgt gcgaggcagc tgcggtaaag
     4741 ctcatcggcg tggtcgtgaa gcgattcaca aatatcggcc tgttcatctg cgtccagttc
     4801 gttgagcttc tccagcagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc
     4861 ggttttttcc tgtttagtca ctgatgcctc cgtgtaaggg ggatttctgt tcatggggta
     4921 atgataccga tgaaacgcga gaggatgctc acaatacggg ttactgatga tgaacatgcc
     4981 cggttacttg aacgctgtga gggtaaacaa ctggcggtat ggatgcggcg ggtctgcctg
     5041 ggggagccgg ttgcccgttc cggaaaactg ccgacactgg caccgccgtt actgcgtcag
     5101 ctggccgcca tcggaaataa cctgaatcag acagcccgta aggtgaacag cgggcagtgg
     5161 tcttccggtg accgggttca ggtggtggcc gcactgatgg ccatcgggga tgagctgcgc
     5221 cggctgcgtc tggccgtcag ggaacagggg gcgcgggatg atagttaaat ttcatgccag
     5281 gggaaaaggt ggtggcagtg gtccggttga ttacctgctg gggagggagc gtaaccgcga
     5341 aggcgcaacg gtgcttcagg gtaatccgga agaagtccgg gaactcatcg atgccacgcc
     5401 atttgcgaag aaatacacgt ccggtgttct gtcgttcgcg gagaaggagc tgccgccggg
     5461 aggacgtgaa aaagtgatgg cgagctttga gcgtgtactg atgcccggtc tcgaaaaaaa
     5521 tcagtacagc atcctgtggg tggagcacca ggacaaggga cggcttgagc tgaattttgt
     5581 cattccgaac atggagctac agaccggaaa acgcctccag ccgtactacg accgcgcaga
     5641 caggcctaga attgatgcct ggcagacgct ggtgaatcac cattacgggc tgcatgaccc
     5701 gaacgccccg gagaaccgca ggacgctgac actccctgat aacctgcctg aaacgaaaca
     5761 ggcgcttgct gagggcgtca cgcgaggtat agatgcactt taccatgccg gagagataaa
     5821 aggccgtcag gatgtgattc aggcgctcac tgaggcgggg ctggaagtgg tcagggtgac
     5881 gcgaagcagt atcagcattg cagatccgaa cggcgggaag aatatcaggc tgaaaggagc
     5941 attttatgag caatcttttg cagacgggcg cggagttcga gaaaaagctg aaagagagag
     6001 ccgaatctac agagaaaatg ctgaacaacg agttcaggag gctcggcgaa tctgtaagcg
     6061 aggctgtgac atcaaacgag acgaaaatca gagacgctat agccctgttc acagcctcga
     6121 cagagaaatc gctggaaaaa caccgggaag gggtgaaaga ggcgatgatg cagcacagga
     6181 gggacgtgtt aaagctggca gggaatacgg gcatgatgtt actggggata gtctttctcc
     6241 tgtttaccgc gagtggcggg acgctctggt atcttggagg gaggatacag gcgaacctgg
     6301 aagaaatcag gaagcaggaa gagacattgc agaaactgaa cgcgaagaca tggggcgtgg
     6361 agtttgtgca ggacgggaac aggaaattcc ttgtccttcc gtacgggaaa tcagcggagg
     6421 tgattccctt tcaggggaaa gagtgggtac atctgaagga gtgacacaga gtgacagagc
     6481 tggaaacaca tttgctgaac gccttagagc agctgcaaca ggactatatg cagcggctga
     6541 gcgaatggga gagcgccttc gtggaattgc agaagatgtt ttcgcttacg caacgggaca
     6601 acgcgatgct gaacgagcgg gtcatgcagt tgagtcagca ggtgcag
//
