LOCUS       NZ_CP079895             6669 bp    DNA     circular CON 02-NOV-2023
DEFINITION  Escherichia fergusonii strain 5zf15-2-1 plasmid p45ZF15-2-1,
            complete sequence.
ACCESSION   NZ_CP079895
VERSION     NZ_CP079895.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20243900
            Assembly: GCF_020002305.1
KEYWORDS    RefSeq.
SOURCE      Escherichia fergusonii
  ORGANISM  Escherichia fergusonii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   Liu,R.
  TITLE     Genomic of Escherichia fergusonii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6669)
  AUTHORS   Liu,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JUL-2021) Zhejiang University, State Key Laboratory
            for Diagnosis and Treatment of Infectious Disease, No. 79 Qingchun
            Road, Hangzhou, Zhejiang 310003, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.10.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/02/2023 04:27:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,110
            CDSs (total)                      :: 4,988
            Genes (coding)                    :: 4,848
            CDSs (with protein)               :: 4,848
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 36 of 140
            Pseudo Genes (incomplete)         :: 93 of 140
            Pseudo Genes (internal stop)      :: 25 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            Pseudo Genes (short protein)      :: 1 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079895.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Escherichia fergusonii"
                     /mol_type="genomic DNA"
                     /strain="5zf15-2-1"
                     /isolation_source="feces"
                     /host="pig"
                     /db_xref="taxon:564"
                     /plasmid="p45ZF15-2-1"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2019-05"
     gene            join(5229..6669,1..128)
                     /gene="cea"
                     /locus_tag="KYD87_RS25470"
                     /old_locus_tag="KYD87_25330"
     CDS             join(5229..6669,1..128)
                     /gene="cea"
                     /locus_tag="KYD87_RS25470"
                     /old_locus_tag="KYD87_25330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            514..651
                     /locus_tag="KYD87_RS25475"
                     /old_locus_tag="KYD87_25340"
     CDS             514..651
                     /locus_tag="KYD87_RS25475"
                     /old_locus_tag="KYD87_25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(850..1008)
                     /locus_tag="KYD87_RS25480"
                     /old_locus_tag="KYD87_25345"
     CDS             complement(850..1008)
                     /locus_tag="KYD87_RS25480"
                     /old_locus_tag="KYD87_25345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(1678..1869)
                     /locus_tag="KYD87_RS25485"
                     /old_locus_tag="KYD87_25350"
     CDS             complement(1678..1869)
                     /locus_tag="KYD87_RS25485"
                     /old_locus_tag="KYD87_25350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1934..2257
                     /locus_tag="KYD87_RS25490"
                     /old_locus_tag="KYD87_25355"
     CDS             1934..2257
                     /locus_tag="KYD87_RS25490"
                     /old_locus_tag="KYD87_25355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2466..>2897
                     /locus_tag="KYD87_RS25580"
                     /pseudo=""
     CDS             2466..>2897
                     /locus_tag="KYD87_RS25580"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2935..3453
                     /locus_tag="KYD87_RS25500"
                     /old_locus_tag="KYD87_25365"
     CDS             2935..3453
                     /locus_tag="KYD87_RS25500"
                     /old_locus_tag="KYD87_25365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_223666489.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWGHLKE"
                     /name="MbeB family mobilization protein"
     gene            3460..3714
                     /locus_tag="KYD87_RS25505"
                     /old_locus_tag="KYD87_25370"
     CDS             3460..3714
                     /locus_tag="KYD87_RS25505"
                     /old_locus_tag="KYD87_25370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015059032.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4032..4451)
                     /locus_tag="KYD87_RS25510"
                     /old_locus_tag="KYD87_25375"
     CDS             complement(4032..4451)
                     /locus_tag="KYD87_RS25510"
                     /old_locus_tag="KYD87_25375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(4532..4969)
                     /locus_tag="KYD87_RS25515"
                     /old_locus_tag="KYD87_25380"
     CDS             complement(4532..4969)
                     /locus_tag="KYD87_RS25515"
                     /old_locus_tag="KYD87_25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     Typing          1506..1593
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          complement(937..1492)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.52"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2248..3818
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.809"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctttactttt tagcttgctt gctggaacta cattaggtat ttggggtatt gctattgtta
       61 caggaattct atgctcctat attgataaga ataaacttaa tactataaat gaggtgttag
      121 ggatttaatt attctttatt gatataaaaa gtcctagcaa tccaaatggg attgctagga
      181 ccaaacaaag tagattatat agcataaata ggtttaattt tgctacgggg gcgttattta
      241 ggttttttct tctttcgaaa aaatctttct ttatgaagtt aaaagctatg tattcaatag
      301 catattttga atatggacat agaatagtgc ttatcactat tgcatatagc atcttatctg
      361 acacaaggaa ataataccct tcgctgtttt ttgttataag gtatatatat ataagtgtgc
      421 agtacaggcc aaataaaata ttttttatgt agtatcttaa gctcataaat taaacctcgc
      481 catatattct tttcatttta taaggatcga gttatgagga aaagattttt tgtgggaata
      541 ttcgcgataa acctccttgt tggatgtcag gctaactata tacgtgatgt tcagggaggg
      601 accatcgcac catcctcctc ttctaaactg acggggatcg cggttcagta gaaaagatca
      661 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
      721 caccgctacc aacggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      781 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagtcgg
      841 gccactactt caagaactct gtagcaccgt ttgtgccatc atcgctctgc taatccggtt
      901 accagtggct gctgccagtg gcgttaaggc gtgccttacc gggttggact caagacgata
      961 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     1021 ggagcgaacg acctacaccg aactgagata ccaacagcgt gagctatgag aaagcgccac
     1081 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     1141 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     1201 ccacctctga cttgagcgtc tatttttgtg atgctcgtca ggggggcgga gcctatggaa
     1261 aaacgcctgc tacgtggcct tcttcctgtt cttggtcttt tgctcacatg ttctttccgg
     1321 ccttatcccc tgattctgtg gataactgtg ttaccgtttt tgtgtgagtc agtaccgctc
     1381 gccgcagtcg aacgaccgag cgtagcgagt cagtgagcga ggaagcggaa aagcgcctgg
     1441 acgtgcattt tctccttacg catctgtgcg gcatttcaca cccggcatgg cgtacttttc
     1501 atacaatccg cactgatgcc gcatggttaa ggcagtatac actccgctat cgctacgtga
     1561 ctgggtcagg gctgcgcccc gacacccgct aaaacctgct gacgcgccct gacgggcttg
     1621 tctgctcccg gcatccgctc acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     1681 gaggttttca ccgtcatccc cgaaacgtgc gaggcagctg cggtaaagct catcggcgtg
     1741 gtcgtgaagc gattcacaga tgtctgcctg ttcatctgcg tccagttcgt tgagcttctc
     1801 cagcagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg
     1861 tttagtcact gatgcctccg tgtaaggggg tatttctatt catggggtaa tgataccgat
     1921 gaaacgcgag aggatgctca cgatacgggt tactgatgat gaacatgcca ggttacttga
     1981 acgctgtgag ggtaaacaac tggcagtatg gatgcggcgg gtctgcctgg gtgagccggt
     2041 tgcccgttcc gggaagctgc cgacactggc accgccgtta ctgcgtcagc tggccgccat
     2101 cgggaataac ctgaatcaga cagcgaggaa ggtgaacagc gggcagtggt cttccggtga
     2161 ccgggttcag gtggtggccg cactgatggc catcggggat gagctgcgcc ggctgcgtct
     2221 ggccgtcagg gaacaggggg cgcgggatga tagttaaatt tcatgccagg ggaaaaggtg
     2281 gtggcagtgg tccggttgat tacctgctgg ggagggagcg taaccgcgaa ggcgcaacgg
     2341 tgcttcaggg taatccggaa gaagtccggg aactcatcga tgccacgcca tttgcgaaga
     2401 aatacacgtc cggtgttctg tcgttcgcgg agaaggagct gccgccggga ggacgtgaaa
     2461 aagtgatggc gagctttgag cgtgtactga tgcccggtct cgaaaaaaat cagtacagca
     2521 tcctgtgggt ggagcaccag gacaagggac ggcttgagct gaattttgtc attccgaaca
     2581 tggagctaca gaccggaaaa cgcctccagc cgtactacga ccgcgcagac aggcctagaa
     2641 ttgatgcctg gcagacgctg gtgaatcacc attacgggct gcatgacccg aacgccccgg
     2701 agaaccgcag gacgctgaca ctccctgata acctgcctga aacgaaacag gcgcttgctg
     2761 agggcgtcac gcgaggtata gatgcacttt accatgccgg agagataaaa ggccgtcagg
     2821 atgtgattca ggcgctcact gaggcggggc tggaagtggt cagggtgacg cgaagcagta
     2881 tcagcattgc agatccgaac ggcgggaaga atatcaggct gaaaggagca ttttatgagc
     2941 aatcttttgc agacgggcgc ggagttcgag aaaaagctga aagagagagc cgaatctaca
     3001 gagaaaatgc tgaacaacga gttcaggagg ctcggcgaat ctgtaagcga ggctgtgaca
     3061 tcaaacgaga cgaaaatcag agacgctata gccctgttca cagcctcgac agaggaatcg
     3121 ctggaaaaac accgggaagg ggtgaaagag gcgatgatgc agcacaggag ggacgtgtta
     3181 aagctggcag ggaatacggg catgatgtta ctggggatag tctttctcct gtttaccgcg
     3241 agtggcggga cgctctggta tcttggaggg aggatacagg cgaacctgga agaaatcagg
     3301 aagcaggaag agacattgca gaaactgaac gcgaagacat ggggcgtgga gtttgtgcag
     3361 gacgggaaca ggaaattcct tgtccttccg tacgggaaat cagcggaggt gattcccttt
     3421 caggggaaag agtggggaca tctgaaggag tgacacagag tgacagagct ggaaacacat
     3481 ttgctgaacg ccttagagca gctgcaacag gactatatgc agcggctgag cgaatgggag
     3541 agcgccttcg tggaattgca gaagatgttt tcgcttacgc aacgggacaa cgcgatgctg
     3601 aacgagcggg tcatgcagtt gagtcagcag gtgcagcact tgagcgagca gacagaacgc
     3661 ttgagcgagc agacagaacg cttgagccag ttatacagcg agaactggag ataagagagg
     3721 aacggctgat acaggagcgc gaacatgtgt tatccctgga acgggagcgt cagccggaaa
     3781 tacaggaacg cacgctggat ggcccttcgc tgggatggtg aaaccatgaa aaatggcagc
     3841 ttcagtggat taagtggggg taatgtggcc tgtaccctct ggttgcatag gtattcatac
     3901 ggttaaaatt tatcaggcgc gatcgcggca gtttttcggg tggtttgttg ccatttttac
     3961 ctgtctgctg ccgtgatcgc gctgaacgcg ttttagcggt gcgtacaatt aagggattat
     4021 ggtaaatcca cttactgtct gccctcgtag ccatcgagat aaaccgcaga aatcgtgtca
     4081 gccagcagcc tggatttttt cggggtaagg tctttgctgc ggtaatcagt acccgcaaat
     4141 tgagttgtgc cccggagtga gtttaaaaat tcctggctgt gaaactgctg aatgcgttgt
     4201 tgcacatcac tccgtgattt tcctgtcgct ttatctttag ctcccatatc ccagaactgc
     4261 cgaaaaaaag gcaccatcat tcgtgttgtg tcggcttttt gggtggaaaa gttgggatca
     4321 aaaccgtcat cagaagcata tacgtaatgc cgggtatgtt gttccacgct gccctttggg
     4381 gtacaggctg tgagagctac tgttgctgtt atcatggcga taagacaaag tttgtttccg
     4441 gtacgtctca tgagtgtctc cctaccttaa agtatttatt ctcggtgagt tattatccga
     4501 agactatttt atcatcagtc tctgacagcg ttcagcgacc gaacaacctg gaccagaatc
     4561 cccgtttctt ttccagttcc agtgcctgcc tgagctgggc tatctcattt tgtagctgtt
     4621 cccgttctgc ttcctggcgt ctgcgatcca tatcctgtgc ctgtttatcc tcaagcatca
     4681 gcgtcaggca ttgtttcagc tcattcagct cccggagaat gcgttctgtc tgctgatcat
     4741 gtggattttc tgcatgtccc tcactgtgcc tttctggtgt ctcattctgc tttaattcgc
     4801 cgtatgcccg gatcagttca ctggtttcaa actcccgtcg tccgtccctg ccggtacggt
     4861 aactgacaag gcctgcgcgc atatcacggt aaaactgtga acgcgatctg cctgtcattt
     4921 ttagtgcgtc ccggagtgtg tgccatgcca taaagtgaca gtgtcccata gatgtctcat
     4981 ctcatagttt cagtaaaaca taatgaggtc tgagaacggt aatgtttgtg ctggtttttg
     5041 tggcatcggg cgagaatagc gcgtggtgtg aaagactgtt tttttgatcg ttttcacaaa
     5101 aatggaagtc cacagtcttg acagggaaaa tgcagcggcg tagcttttat gctgtatata
     5161 aaaccagtgg ttatatgtac agtatttatt tttaacttat tgttttaaaa gtcaaagagg
     5221 attttataat ggaaaccgcg gtagcgtact ataaagatgg tgttccttat gatgataagg
     5281 gacaggtaat tattactctt ttgaatggta ctcctgacgg gagtggctct ggcggcggag
     5341 gtggaaaagg aggcagtaaa agtgaaagtt ctgcagctat tcatgcaact gctaaatggt
     5401 ctactgctca attaaagaaa acacaggcag agcaggctgc ccgggcaaaa gctgcagcgg
     5461 aagcacaggc gaaagcaaag gcaaacaggg atgcgctgac tcagcgcctg aaggatatcg
     5521 tgaatgaggc tcttcgtcac aatgcctcac gtacgccttc agcaacagag cttgctcatg
     5581 ctaataatgc agctatgcag gcggaagcag agcgtttgcg ccttgcgaaa gcagaagaaa
     5641 aagcccgtaa agaagcggaa gcagcagaaa aggcttttca ggaagcagaa caacgacgta
     5701 aagagattga acgggagaag gctgaaacag aacgccagtt gaaactggct gaagctgaag
     5761 agaaacgact ggctgcattg agtgaagaag ctaaagctgt tgagatcgcc caaaaaaaac
     5821 tttctgctgc acaatctgaa gtggtgaaaa tggatggaga gattaagact ctcaattctc
     5881 gtttaagctc cagtatccat gcccgtgatg cagaaatgaa aacgctcgct ggaaaacgaa
     5941 atgaactggc tcaggcatcc gctaaatata aagaactgga tgagctggtc aaaaaactat
     6001 caccaagagc caatgatccg cttcagaacc gtcctttttt tgaagcaacc agacgacggg
     6061 ttggggccgg taagattaga gaagaaaaac aaaaacaggt aacagcatca gaaacacgta
     6121 ttaaccggat aaatgctgat ataactcaga tccagaaggc tatttctcag gtcagtaata
     6181 atcgtaatgc cggtatcgct cgtgttcatg aagctgaaga aaatttgaaa aaagcacaga
     6241 ataatctcct taattcacag attaaggatg ctgttgatgc aacagttagc ttttatcaaa
     6301 cgctgactga aaaatatggt gaaaaatatt cgaaaatggc acaggaactt gctgataagt
     6361 ctaaaggtaa gaaaatcggc aatgtgaatg aagctctcgc tgcttttgaa aaatacaagg
     6421 atgttttaaa taagaaattc agcaaagccg atcgtgatgc tatttttaat gcgttggcat
     6481 cggtgaagta tgatgactgg gctaaacatt tagatcagtt tgccaagtac ttgaagatta
     6541 cggggcatgt ttcttttgga tatgatgtgg tatctgatat cctaaaaatt aaggatacag
     6601 gtgactggaa gccactattt cttacattag agaagaaagc tgcagatgca ggggtgagtt
     6661 atgttgttg
//
