LOCUS       NZ_CP079888             9354 bp    DNA     circular CON 02-NOV-2023
DEFINITION  Escherichia fergusonii strain 6S41-1 plasmid p4-6S41-1, complete
            sequence.
ACCESSION   NZ_CP079888
VERSION     NZ_CP079888.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20239770
            Assembly: GCF_020002285.1
KEYWORDS    RefSeq.
SOURCE      Escherichia fergusonii
  ORGANISM  Escherichia fergusonii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9354)
  AUTHORS   Liu,R.
  TITLE     Genomic of Escherichia fergusonii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9354)
  AUTHORS   Liu,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JUL-2021) Zhejiang University, State Key Laboratory
            for Diagnosis and Treatment of Infectious Disease, No. 79 Qingchun
            Road, Hangzhou, Zhejiang 310003, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.10.0
            Assembly Name                     :: 6S41-1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Oxford Nanopore; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/02/2023 04:16:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,919
            CDSs (total)                      :: 4,804
            Genes (coding)                    :: 4,637
            CDSs (with protein)               :: 4,637
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 167
            CDSs (without protein)            :: 167
            Pseudo Genes (ambiguous residues) :: 0 of 167
            Pseudo Genes (frameshifted)       :: 35 of 167
            Pseudo Genes (incomplete)         :: 118 of 167
            Pseudo Genes (internal stop)      :: 32 of 167
            Pseudo Genes (multiple problems)  :: 18 of 167
            Pseudo Genes (short protein)      :: 1 of 167
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079888.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9354
                     /organism="Escherichia fergusonii"
                     /mol_type="genomic DNA"
                     /strain="6S41-1"
                     /isolation_source="soil"
                     /db_xref="taxon:564"
                     /plasmid="p4-6S41-1"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2019-05"
     gene            join(9253..9354,1..318)
                     /locus_tag="KYC43_RS24405"
                     /old_locus_tag="KYC43_24290"
     CDS             join(9253..9354,1..318)
                     /locus_tag="KYC43_RS24405"
                     /old_locus_tag="KYC43_24290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            684..1361
                     /gene="tnpA"
                     /locus_tag="KYC43_RS24410"
                     /old_locus_tag="KYC43_24295"
     CDS             684..1361
                     /gene="tnpA"
                     /locus_tag="KYC43_RS24410"
                     /old_locus_tag="KYC43_24295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="WP_001682408.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKSTV
                     CSMFVRFRRAGLSWPLPAGMSEQELDACLYGQFSTVPVVRPESTVISEAPMVKKRPRRP
                     NFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPALLP
                     VTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIREIKG
                     SSH"
                     /name="tnpA"
     gene            1361..1708
                     /gene="tnpB"
                     /locus_tag="KYC43_RS24415"
                     /old_locus_tag="KYC43_24300"
     CDS             1361..1708
                     /gene="tnpB"
                     /locus_tag="KYC43_RS24415"
                     /old_locus_tag="KYC43_24300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="WP_000624622.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLFI
                     FRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINWKH
                     PKRTERAGIRI"
                     /name="tnpB"
     gene            1728..3299
                     /locus_tag="KYC43_RS24420"
                     /old_locus_tag="KYC43_24305"
     CDS             1728..3299
                     /locus_tag="KYC43_RS24420"
                     /old_locus_tag="KYC43_24305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISCro1 family transposase"
                     /protein_id="WP_000381395.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEIN
                     RLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPSAL
                     RQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIETQR
                     PKQACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQGVEL
                     SRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTARLWVYV
                     RDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESGRITEAA
                     CMAHARRKIHDVHARAPTYITTEALQRIGELYAIEAEVRGCSAEQRLAARKARAAPLMQ
                     SLYDWIQQQMKTLSRHSDTAKAFAYLLKQWDALNVYCSNGWVEIDNNIAENALRGVAVG
                     RKNWMFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPANRVRDLLPWK
                     VDLSSQ"
                     /name="IS66-like element ISCro1 family transposase"
     gene            complement(3343..3576)
                     /locus_tag="KYC43_RS24425"
                     /old_locus_tag="KYC43_24310"
     CDS             complement(3343..3576)
                     /locus_tag="KYC43_RS24425"
                     /old_locus_tag="KYC43_24310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3583..4101)
                     /locus_tag="KYC43_RS24430"
                     /old_locus_tag="KYC43_24315"
     CDS             complement(3583..4101)
                     /locus_tag="KYC43_RS24430"
                     /old_locus_tag="KYC43_24315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4139..4837)
                     /locus_tag="KYC43_RS24675"
                     /old_locus_tag="KYC43_24320"
                     /pseudo=""
     CDS             complement(<4139..4837)
                     /locus_tag="KYC43_RS24675"
                     /old_locus_tag="KYC43_24320"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4779..5102)
                     /locus_tag="KYC43_RS24440"
                     /old_locus_tag="KYC43_24325"
     CDS             complement(4779..5102)
                     /locus_tag="KYC43_RS24440"
                     /old_locus_tag="KYC43_24325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5166..5357
                     /locus_tag="KYC43_RS24445"
                     /old_locus_tag="KYC43_24330"
     CDS             5166..5357
                     /locus_tag="KYC43_RS24445"
                     /old_locus_tag="KYC43_24330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6027..6185
                     /locus_tag="KYC43_RS24680"
     CDS             6027..6185
                     /locus_tag="KYC43_RS24680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(6384..6521)
                     /locus_tag="KYC43_RS24450"
                     /old_locus_tag="KYC43_24335"
     CDS             complement(6384..6521)
                     /locus_tag="KYC43_RS24450"
                     /old_locus_tag="KYC43_24335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            6683..6910
                     /locus_tag="KYC43_RS24455"
                     /old_locus_tag="KYC43_24340"
     CDS             6683..6910
                     /locus_tag="KYC43_RS24455"
                     /old_locus_tag="KYC43_24340"
                     /inference="COORDINATES: protein motif:HMM:NF015489.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_229471606.1"
                     /translation="MLYAIVISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(6907..8475)
                     /gene="cea"
                     /locus_tag="KYC43_RS24460"
                     /old_locus_tag="KYC43_24345"
     CDS             complement(6907..8475)
                     /gene="cea"
                     /locus_tag="KYC43_RS24460"
                     /old_locus_tag="KYC43_24345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            8735..9172
                     /locus_tag="KYC43_RS24465"
                     /old_locus_tag="KYC43_24350"
     CDS             8735..9172
                     /locus_tag="KYC43_RS24465"
                     /old_locus_tag="KYC43_24350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     Typing          complement(5443..5530)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          5541..6099
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.722"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3372..4789)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.789"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 cattacgtat atgcttctga tgacggtttt gatcccaact tttccaccca aaaagccgac
       61 acaacacgaa tgatggtgcc tttttttcgg cagttctggg atatgggagc taaagataaa
      121 gcgacaggaa aatcacggag tgatgtgcaa caacgcattc agcagtttca cagccaggaa
      181 tttttaaact cactccgggg cacaactcaa tttgcgggta ctgattaccg cagcaaagac
      241 cttaccccga aaaaatccag gctgctggct gacacgattt ctgcggttta tctcgatggc
      301 tacgagggca gacagtaagt ggatttacca taatccctta attgtacgca ccgctaaaac
      361 gcgttcagcg cgatcacggc agcagacagg taaaaatggc aacaaaccac ccgaaaaact
      421 gccgcgatcg cgcctgataa attttaaccg tatgaatacc tatgcaacca gagggtacag
      481 gccacattac ccccacttaa tccactgaag ctgccatttt tcatggtttc accatcccag
      541 cgaagggcca tccagcgtgc gttcctgtat ttccggctga cgctcccgtt ccagggataa
      601 cacatgttcg cgctcctgta tcagccgttc gtaaccggct catttaaacc gtctggtctg
      661 tttcctccgg ctctacaaaa ataatgtcca tcatttttaa tggacactat cgtatgaaac
      721 accggacctg gatcactgaa gctttacgtc ttcactttga agaacattta ccccgggttg
      781 tggccgggcg tcgcctgggt gtaccaaaat caacagtttg tagtatgttc gtgcgctttc
      841 ggagagctgg cctttcgtgg cctttgcccg caggcatgtc ggagcaggaa cttgatgcct
      901 gcctttacgg acaattttcc acggtaccag tcgtacgtcc tgaaagcacc gttatatccg
      961 aagcccccat ggtaaaaaaa cgtccccggc ggcccaactt cccttatgag tttaaaatcg
     1021 ccttagtgga gcagtcactg cagcccggag cctgtgtggc gcagatcgcc cgggaaaacg
     1081 gaatcaacga taacctgctc ttcaactggc gccatcaata ccggaaaggt ggcctgctgc
     1141 cttccggaaa aaatatgccg gcactgcttc ccgtgacgtt aacgccggag ccggataata
     1201 aaatcccggc ccccgcacag gaaccagagc agataaatac accgtccgac agtctgtgtt
     1261 gtgagctggt tctgccggcc ggaactctca ggcttaaagg taaactgacg ccggcgttat
     1321 tacagacact tatccgcgaa ataaaaggga gcagccactg atgatatctc tccctgcagg
     1381 ttcgcgtatc tggctggttg caggtatcac cgatatgcga aatggcttta acggcctggc
     1441 atcaaaagtt cagaacgtcc tgaaggatga cccgttctcc ggacacctgt tcatcttccg
     1501 cggacgccgg ggtgaccaga taaaagtgtt gtgggctgac agtgacggac tgtgcctctt
     1561 caccaaacgc ctggagcggg gccgcttcgt ctggccagtc acccgtgacg gcaaggtgca
     1621 ccttactccg gctcagttat ccatgcttct tgaaggtatc aactggaagc acccgaaacg
     1681 aacggaacgc gctggaatcc gcatataacc cgttgtaaag tgaggatatg gacacctcac
     1741 ttgctcatga gaacgcccgc ctgcgggcac tgttgcagac gcaacaggac accatccgcc
     1801 agatggctga atacaaccgc ctgctctcac agcgggtggc ggcttatgct tccgaaatca
     1861 accggctgaa ggcgctggtt gcgaaactgc aacgtatgca gttcggtaaa agctcagaaa
     1921 aacttcgtgc aaaaaccgaa cggcagatac aggaagcaca ggagcgaatc agcgcacttc
     1981 aggaagaaat ggcggaaacg ctgggtgagc aatatgaccc ggtactgcca tccgccctgc
     2041 gccagtcttc agcccgtaaa ccgttaccgg cctcacttcc ccgtgaaacc cgggttatcc
     2101 ggccggaaga ggaatgctgt cctgcctgtg gtggtgaact cagttctctg ggatgtgatg
     2161 tgtcagagca actggagctt atcagcagcg cctttaaggt tatcgaaaca caacgtccga
     2221 aacaggcctg ttgccggtgc gaccatatcg tgcaggcacc agtaccttca aaacccattg
     2281 cacgcagtta tgccggagcg gggcttctgg cccatgttgt caccgggaaa tatgcagacc
     2341 atctgccgtt ataccgccag tcagaaatat accgtcgtca gggagtggag ctgagccgtg
     2401 ccacactggg gcgctggaca ggtgctgttg ctgaactgct ggagccgctg tatgacgtcc
     2461 tgcgccagta tgtgctgatg cccggtaaag tccatgctga tgatatcccc gtcccggtcc
     2521 aggagccggg cagcggtaaa acccggacag cccggctgtg ggtctacgtc cgtgatgacc
     2581 gtaacgccgg ttcacagatg cccccggcgg tctggttcgc gtacagtccg gaccggaaag
     2641 gtatccatcc acaaaatcac ctggccggtt acagcggtgt gcttcaggcc gatgcttacg
     2701 gtggttaccg ggcgttatac gaatccggca gaataacgga agccgcgtgt atggctcatg
     2761 cccggagaaa aatccacgat gtgcatgcaa gagcgcccac ctacatcacc acggaagccc
     2821 tgcagcgtat cggtgaactg tatgccatcg aggcagaggt ccggggctgt tcagcagaac
     2881 agcgtctggc ggcaagaaaa gccagagccg cgccactgat gcagtcactg tatgactgga
     2941 tacagcaaca gatgaaaaca ctgtcgcgtc actcagatac ggcaaaagcg ttcgcatacc
     3001 tgctgaaaca gtgggatgca ctgaacgtgt actgcagtaa tggctgggtg gaaatcgaca
     3061 acaacatcgc agagaacgcc ttacggggag tggccgtagg ccggaaaaac tggatgttcg
     3121 cgggttccga cagcggtggt gaacatgcgg cggtgttgta ctcgctgatc ggcacatgcc
     3181 gtctgaacaa tgtggagcca gaaaagtggc tgcgttacgt cattgaacat atccaggact
     3241 ggccggcaaa ccgggtacgc gatctgttgc cctggaaagt tgatctgagc tctcagtaaa
     3301 tatcaatacg gttctgacga gtcgcttaca gccgttcctc tcttatctcc agttctcgct
     3361 gtataactgg ctcaagcgtt ctgtctgctc gctcaagtgc tgcacctgct gactcaactg
     3421 catgacccgc tcgttcagca tcgcgttgtc ccgttgcgta agcgaaaaca tcttctgcaa
     3481 ttccacgaag gcgctctccc attcgctcag ccgctgcata tagtcctgtt gcagctgctc
     3541 taaggcgttc agcaaatgtg tttccagctc tgtcactctg tgtcactcct tcagatgtac
     3601 ccactctttc ccctgaaagg gaatcacctc cgctgatttc ccgtacggaa ggacaaggaa
     3661 tttcctgttc ccgtcctgca caaactccac gccccatgtc ttcgcgttca gtttctgcaa
     3721 tgtctcttcc tgcttcctga tttcttccag gttcgcctgt atcctccctc caagatacca
     3781 gagcgtcccg ccactcgcgg taaacaggag aaagactatc cccagtaaca tcatgcccgt
     3841 attccctgcc agctttaaca cgtccctcct gtgctgcatc atcgcctctt tcaccccttc
     3901 ccggtgtttt tccagcgatt cctctgtcga ggctgtgaac agggctatag cgtctctgat
     3961 tttcgtctcg tttgatgtca cagcctcgct tacagattcg ccgagcctcc tgaactcgtt
     4021 gttcagcatt ttctctgtag attcggctct ctctttcagc tttttctcga actccgcgcc
     4081 cgtctgcaaa agattgctca taaaatgctc ctttcagcct gatattcttc ccgccgttcg
     4141 gatctgcaat gctgatactg cttcgcgtca ccctgaccac ttccagcccc gcctcagtga
     4201 gcgcctgaat cacatcctga cggcctttta tctctccggc atggtaaagt gcatctatac
     4261 ctcgcgtgac gccctcagca agcgcctgtt tcgtttcagg caggttatca gggagtgtca
     4321 gcgtcctgcg gttctccggg gcgttcgggt catgcagccc gtaatggtga ttcaccagcg
     4381 tctgccaggc atcaattcta ggcctgtctg cgcggtcgta gtacggctgg aggcgttttc
     4441 cggtctgtag ctccatgttc ggaatgacaa aattcagctc aagccgtccc ttgtcctggt
     4501 gctccaccca caggatgctg tactgatttt tttcgagacc gggcatcagt acacgctcaa
     4561 agctcgccat cactttttca cgtcctcccg gcggcagctc cttctccgcg aacgacagaa
     4621 caccggacgt gtatttcttc gcaaatggcg tggcatcgat gagttcccgg acttcttccg
     4681 gattaccctg aagcaccgtt gcgccttcgc ggttacgctc cctccccagc aggtaatcaa
     4741 ccggaccact gccaccacct tttcccctgg catgaaattt aactatcatc ccgcgccccc
     4801 tgttccctga cagccagacg cagccggcgc agctcatccc cgatggccat cagtgcggcc
     4861 accacctgaa cccggtcacc ggaagaccac tgcccgctgt tcaccttacg ggctgtctga
     4921 ttcaggttat ttccgatggc ggccagctga cgcagtaacg gcggtgccag tgtcggcagt
     4981 tttccggaac gggcaaccgg ctcccccagg cagacccgcc gcatccatac cgccagttgt
     5041 ttaccctcac agcgttcaag taaccgggca tgttcatcat cagtaacccg tattgtgagc
     5101 atcctctcgc gtttcatcgg tatcattacc ccatgaacag aaatccccct tacacggagg
     5161 catcagtgac taaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga
     5221 cattaacgct gctggagaag ctcaacgaac tggacgcaga tgaacaggcc gatatttgtg
     5281 aatcgcttca cgaccacgcc gatgagcttt accgcagctg cctcgcacgt ttcggggatg
     5341 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtgagcgg
     5401 atgccgggag ctgacaagcc cgtcagggcg cgtcagcagg ttttagcggg tgtcggggcg
     5461 cagccctgac ccagtcacgt agcgatagcg gagtgtatac tggcttaacc atgcggcatc
     5521 agtgcggatt gtatgaaaag tacgccatgc cgggtgtgaa atgccgcaca gatgcgtaag
     5581 gagaaaatgc acgtccaggc gcttttccgc ttcctcgctc actgactcgc tacgctcggt
     5641 cgttcgactg cggcgagcgg tactgactca cacaaaaacg gtaacacagt tatccacaga
     5701 atcaggggat aaggccggaa agaacatgtg agcaaaagac caggaacagg aagaaggcca
     5761 cgtagcaggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     5821 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     5881 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     5941 tcccttcggg aagcgtggcg ctttctcata gctcacgctg ttggtatctc agttcggtgt
     6001 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6061 ccttatccgg taactatcgt cttgagtcca acccggtaag gcacgcctta acgccactgg
     6121 cagcagccac tggtaaccgg attagcagag cgatgatggc acaaacggtg ctacagagtt
     6181 cttgaagtag tggcccgact acggctacac tagaaggaca gtatttggta tctgcgctct
     6241 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     6301 cgttggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     6361 tcaagaagat cctttgatct tttctactga accgcgatcc ccgtcagttt agaagaggag
     6421 gatggtgcga tggtccctcc ctgaacatca cgtatatagt tagcctgaca tccaacaagg
     6481 aggtttatcg cgaatattcc cacaaaaaat cttttcctca taactcgatc cttataaaat
     6541 gaaaagaata tatggcgagg tttaatttat gagcttaaga tactacataa aaaatatttt
     6601 atttggcctg tactgcacac ttatatatat ataccttata acaaaaaaca gcgaagggta
     6661 ttatttcctt gtgtcagata agatgctata tgcaatagtg ataagcacta ttctatgtcc
     6721 atattcaaaa tatgctattg aatacatagc ttttaacttc ataaagaaag attttttcga
     6781 aagaagaaaa aacctaaata acgcccccgt agcaaaatta aacctattta tgctatataa
     6841 tctactttgt ttggtcctag caatcccatt tggattgcta ggacttttta tatcaataaa
     6901 gaataattaa atccctaaca cctcatttat agtattaagt ttattcttat caatatagga
     6961 gcatagaatt cctgtaacaa tagcaatacc ccaaatacct aatgtagttc cagcaagcaa
     7021 gctaaaaagt aaagcaacaa cataactcac ccctgcatct gcagctttct tctctaatgt
     7081 aagaaatagt ggcttccagt cacctgtatc cttaattttt aggatatcag ataccacatc
     7141 atatccaaaa gaaacatgcc ccgtaatctt caagtacttg gcaaactgat ctaaatgttt
     7201 agcccagtca tcatacttca ccgatgccaa cgcattaaaa atagcatcac gatcggcttt
     7261 gctgaatttc ttatttaaaa catccttgta tttttcaaaa gcagcgagag cttcattcac
     7321 attgccgatt ttcttacctt tagacttatc agcaagttcc tgtgccattt tcgaatattt
     7381 ttcaccatat ttttcagtca gcgtttgata aaagctaact gttgcatcaa cagcatcctt
     7441 aatctgtgaa ttaaggagat tattctgtgc ttttttcaaa ttttcttcag cttcatgaac
     7501 acgagcgata ccggcattac gattattact gacctgagaa atagccttct ggatctgagt
     7561 tatatcagca tttatccggt taatacgtgt ttctgatgct gttacctgtt tttgtttttc
     7621 ttctctaatc ttaccggccc caacccgtcg tctggttgct tcaaaaaaag gacggttctg
     7681 aagcggatca ttggctcttg gtgatagttt tttgaccagc tcatccagtt ctttatattt
     7741 agcggatgcc tgagccagtt catttcgttt tccagcgagc gttttcattt ctgcatcacg
     7801 ggcatggata ctggagctta aacgagaatt gagagtctta atctctccat ccattttcac
     7861 cacttcagat tgtgcagcag aaagtttttt ttgggcgatc tcaacagctt tagcttcttc
     7921 actcaatgca gccagtcgtt tctcttcagc ttcagccagt ttcaactggc gttctgtttc
     7981 agccttctcc cgttcaatct ctttacgtcg ttgttctgct tcctgaaaag ccttttctgc
     8041 tgcttccgct tctttacggg ctttttcttc tgctttcgca aggcgcaaac gctctgcttc
     8101 cgcctgcata gctgcattat tagcatgagc aagctctgtt gctgaaggcg tacgtgaggc
     8161 attgtgacga agagcctcat tcacgatatc cttcaggcgc tgagtcagcg catccctgtt
     8221 tgcctttgct ttcgcctgtg cttccgctgc agcttttgcc cgggcagcct gctctgcctg
     8281 tgttttcttt aattgagcag tagaccattt agcagttgca tgaatagctg cagaactttc
     8341 acttttactg cctccttttc cacctccgcc gccagagcca ctcccgtcag gagtaccatt
     8401 caaaagagta ataattacct gtcccttatc atcataagga acaccatctt tatagtacgc
     8461 taccgcggtt tccattataa aatcctcttt gacttttaaa acaataagtt aaaaataaat
     8521 actgtacata taaccactgg ttttatatac agcataaaag ctacgccgct gcattttccc
     8581 tgtcaagact gtggacttcc atttttgtga aaacgatcaa aaaaacagtc tttcacacca
     8641 cgcgctattc tcgcccgatg ccacaaaaac cagcacaaac attaccgttc tcagacctca
     8701 ttatgtttta ctgaaactat gagatgagac atctatggga cactgtcact ttatggcatg
     8761 gcacacactc cgggacgcac taaaaatgac aggcagatcg cgttcacagt tttaccgtga
     8821 tatgcgcgca ggccttgtca gttaccgtac cggcagggac ggacgacggg agtttgaaac
     8881 cagtgaactg atccgggcat acggcgaatt aaagcagaat gagacaccag aaaggcacag
     8941 tgagggacat gcagaaaatc cacatgatca gcagacagaa cgcattctcc gggagctgaa
     9001 tgagctgaaa caatgcctga cgctgatgct tgaggataaa caggcacagg atatggatcg
     9061 cagacgccag gaagcagaac gggaacagct acaaaatgag atagcccagc tcaggcaggc
     9121 actggaactg gaaaagaaac ggggattctg gtccaggttg ttcggtcgct gaacgctgtc
     9181 agagactgat gataaaatag tcttcggata ataactcacc gagaataaat actttaaggt
     9241 agggagacac tcatgagacg taccggaaac aaactttgtc ttatcgccat gataacagca
     9301 acagtagctc tcacagcctg taccccaaag ggcagcgtgg aacaacatac ccgg
//
