LOCUS       NZ_CP079815             2581 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC02_S465MC plasmid
            pS465MC_p8, complete sequence.
ACCESSION   NZ_CP079815
VERSION     NZ_CP079815.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20115065
            Assembly: GCF_019334705.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC02_S465MC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC02_S465MC
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 91.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:44:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,688
            CDSs (total)                      :: 5,562
            Genes (coding)                    :: 5,417
            CDSs (with protein)               :: 5,417
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 61 of 145
            Pseudo Genes (incomplete)         :: 79 of 145
            Pseudo Genes (internal stop)      :: 24 of 145
            Pseudo Genes (multiple problems)  :: 17 of 145
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079815.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC02_S465MC"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pS465MC_p8"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2014"
                     /collected_by="Christian medical college"
     gene            complement(86..832)
                     /gene="istB"
                     /locus_tag="KWL80_RS28295"
                     /old_locus_tag="KWL80_28270"
     CDS             complement(86..832)
                     /gene="istB"
                     /locus_tag="KWL80_RS28295"
                     /old_locus_tag="KWL80_28270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="WP_001016257.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTAE
                     ELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNILLT
                     GATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNSDLL
                     LLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAILDRL
                     VHGSIKIELKGESMRKIQSPLTEGDQ"
                     /name="istB"
     gene            complement(847..2388)
                     /gene="istA"
                     /locus_tag="KWL80_RS28300"
                     /old_locus_tag="KWL80_28275"
     CDS             complement(847..2388)
                     /gene="istA"
                     /locus_tag="KWL80_RS28300"
                     /old_locus_tag="KWL80_28275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="WP_002431311.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRYANR
                     AAQLGIKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLWEE
                     YCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPETGEI
                     RTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSATSRA
                     CKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHEIFYSL
                     ASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKKVRVHAD
                     YHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYSTRNEHMPE
                     AHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRPHPEQSYRFCLGLLNLHKKYSKAR
                     LNAACARALETKVWRLSGIKSILEKGLDKQPVQDPKPDLLSTMEHENVRGSEYYH"
                     /name="istA"
ORIGIN
        1 tgcgtattac cgtgaaggag atcggtgagt aacatcgatg gagatcggtt cgtgtcactt
       61 tcacagaacc gtttttaaat taccttcact gatctccttc ggtcaacgga gattgtattt
      121 tccgcattga ttctccttta agttcgatct tgatactgcc atgaaccagt cgatctagga
      181 tggcatccgc atgtgtggag tctccgatca ttttgtacca gttttccacc ggcaactggc
      241 tcactacgat gattgagcct cgttggtaca tcagatccac tatttccagc aggtcgctac
      301 gctgttctga tgagagaggt tccagccccc agtcatccag aagcagcaga tcgctattat
      361 tcagcctggt caaaagtttg ctgtatcttc catcagcatg cccctgatag cactgttcca
      421 tcagcgcttt aaggcgataa tagtagacct tgtatccctg tcggcaggca ttatgaccaa
      481 gtgcacatgc caggaacgtt ttaccgctgc cggtggcccc ggtcagtaaa atattttgtt
      541 tcagggttaa ccagtttccc tgactgagtg aacggatgag ggccctgtcc agccctctat
      601 tgttacgata atccagcttt gataactcag cattaagtct gaaccgtgca tgtttgatca
      661 gacgctctgc cttcctgttt tcacggcagg ttagttcttc tgctgtcagt aatgacaggc
      721 gttcttcgaa gcccagctcc tggtatgtgc ccggttgagc aagttgcttt ttaagcgcat
      781 cacggaagcc ggtgagttta agtgcggtca gttgttcgta aagatgattc atcattggat
      841 cccgtatcag tggtaatact cactgccgcg tacgttttcg tgttccatcg tggataacag
      901 atctggtttt ggatcctgaa caggttgttt atccagacct ttttccagga tcgatttaat
      961 acctgacaga cgccatacct ttgtttccag agctcttgca catgctgcat taagtctggc
     1021 tttactgtat tttttatgaa ggttcaggag tccaaggcag aagcgatagc tttgttccgg
     1081 atgtggacga gagttcagta tatgaagcac ataactatga gtttcactgc ctatgtgccc
     1141 cgcccattcc agaagacgct ctggcgtcca ggtggcatgc tgtcgatgag cttcaggcat
     1201 gtgctcgttg cgggtactgt agccataagt acgcttgcgc gggtgcacag caacctcctg
     1261 cccctgattg aagagtctta ccagttctcc ggagatccat gcttccagtt gctggcctaa
     1321 cagcgaacat ggaaccgagt agtaatgttt gtcgatttcc acgtggtaat cggcatgaac
     1381 tctgactttc ttcaccaggg tgtaactgta actggcttca ggaagaggct tcagtgcggg
     1441 tttatcaagc tggatgaaga gttctgcacg tgaataaccc aacttctgca ttattttgtt
     1501 attcagtctt tccagcaact cccgaatgcg ctgattaagc gatgcaaggc tgtagaagat
     1561 ctcatgcctg attcgggcca tgatccagcg ttcaacaacc tgaacgccaa cttcagcttt
     1621 ggctttatct ttcggtttac gtggccgcgc aggcaaaact gcgacattat aatgctcaag
     1681 catctgctgg taggtagggt taacgtcagg atcatactta catgccctgg atgtggcgct
     1741 tttcagattg tccggaacaa caagttcagg aacgccaccc aaccactgga agcagcgaac
     1801 atgactcatc acccagtctt caagctgctg agaccaggtg gcctctgccc atgtgtaact
     1861 tgatgccccg agaacagcta cgatgacctg agcagttctt atttctccgg tctcagggtc
     1921 ggtaacgcca acggtaggtc cacagtaatc aacgaaaagt ttttcgccag ctttatgtac
     1981 ctgacgcatt gatggtgaag tggttttgag ccattcacgg tacatccggc agtaatggtt
     2041 atagctgtaa aaaccgcctg gattacgctc acagtattct tcccagagta gctgcagcgt
     2101 cacgcattta ttacgcagtt cccggtgtac tgtagcccag tcaggcagag agtgcttctt
     2161 catcttaacc tgggtctgaa ggaacgcatg ttttagtttt gtatcatccc atcctgtagg
     2221 taagggccac tgctttatgc caagttgagc cgcccgatta gcatatctgg atacaacgga
     2281 aggggagatt gcaagactac gaccaatttg tcgatggctg agtccaacac cgtatttaag
     2341 cctaagaatt tctttaagtt ttctcataga aattggaact gttggcatag gtatccttta
     2401 ccggaatggc aaaagataca gatcaacaca cctgtgaagt tccaataaca ttgatggaga
     2461 tcactgaata acaaaatgag tcaaaagtga tctccatcga tgttattcag cgatctgttc
     2521 aaatgttatt acccgatctc gatggaagtt attgagtgat ctcctttcat gaaaatacgc
     2581 a
//
