LOCUS       NZ_CP079814             2710 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC02_S465MC plasmid
            pS465MC_p7, complete sequence.
ACCESSION   NZ_CP079814
VERSION     NZ_CP079814.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20115065
            Assembly: GCF_019334705.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC02_S465MC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC02_S465MC
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 91.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:44:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,688
            CDSs (total)                      :: 5,562
            Genes (coding)                    :: 5,417
            CDSs (with protein)               :: 5,417
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 61 of 145
            Pseudo Genes (incomplete)         :: 79 of 145
            Pseudo Genes (internal stop)      :: 24 of 145
            Pseudo Genes (multiple problems)  :: 17 of 145
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079814.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC02_S465MC"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pS465MC_p7"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2014"
                     /collected_by="Christian medical college"
     gene            complement(149..1009)
                     /locus_tag="KWL80_RS28280"
                     /old_locus_tag="KWL80_28255"
     CDS             complement(149..1009)
                     /locus_tag="KWL80_RS28280"
                     /old_locus_tag="KWL80_28255"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     gene            complement(1192..1749)
                     /locus_tag="KWL80_RS28285"
                     /old_locus_tag="KWL80_28260"
     CDS             complement(1192..1749)
                     /locus_tag="KWL80_RS28285"
                     /old_locus_tag="KWL80_28260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001235713.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            1913..>2710
                     /locus_tag="KWL80_RS28290"
                     /old_locus_tag="KWL80_28265"
                     /pseudo=""
     CDS             1913..>2710
                     /locus_tag="KWL80_RS28290"
                     /old_locus_tag="KWL80_28265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /name="DUF4158 domain-containing protein"
     ARG             complement(153..1009)
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
       61 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
      121 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
      181 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
      241 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
      301 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
      361 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
      421 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
      481 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
      541 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
      601 gaagtaagtt ggcagcagtg ttatcactca tggttatggc agcactgcat aattctctta
      661 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
      721 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
      781 caccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
      841 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
      901 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
      961 atgccgcaaa aaagggaata agggcgacac gaaaatgttg aatactcata ctcttccttt
     1021 ttcaatatta ttgaagcatt taccagggtt attgtctcat gagcggatac atatttgaat
     1081 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     1141 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     1201 cctttcgtct tcaagaattt tataaaccgt ggagcgggca atactgagct gatgagcaat
     1261 ttccgttgca ccagtgccct tctgatgaag cgtcagcacg acgttcctgt ccacggtacg
     1321 cctgcggcca aatttgattc ctttcagctt tgcttcctgt cggccctcat tcgtgcgttc
     1381 taggatcctc cggcgttcag cctgtgccac agccgacagg atggtgacca ccatttgccc
     1441 catatcaccg tcggtactga tcccgtcatc aatgaaccgg actgccacgc cctgagcgtc
     1501 aaattccttt atcagttgga tcatatcggc agtgtcgcgg ccaagacggt cgagcttctt
     1561 aaccagaatg acatcacctt cctccacctt catcctcagc aaatccagcc cttcccggtc
     1621 tgttgaactg ccggatgcct tatcggtaaa tatacggttt gctttcacac ctgcgtcttt
     1681 gagtgctctg acctgaagat caagagactg ctgactggtt gagacccgag cgtaaccaaa
     1741 aagtcgcata aaaatgtacc ttaaatcgaa tatcggacaa ctcatgtcta ttattacaaa
     1801 tttacgattt aatagacata ttaatgtaac agttttacga tgtccgataa tttataacat
     1861 ttcgtacggt tggaaaaatg ttactaaatg cccgtcaggc agggaggccg atatgcccgt
     1921 tgactttctg accactgagc agactgaaag ctatggcaga ttcaccggtg aaccggatga
     1981 gcttcagctg gcacgatatt ttcaccttga tgaagcagac aaggaattta tcggaaaaag
     2041 cagaggtgat cacaaccgtc tgggcattgc cctgcaaatt ggatgtgtcc gttttctggg
     2101 caccttcctc accgatatga atcatattcc ttccggcgtc cggcatttta ccgccagaca
     2161 gctcgggatt cgtgatatca ccgttcttgc agaatacggt cagagggaaa atacccgccg
     2221 tgagcatgca gcgctgatac gtcagcacta tcagtatcgt gaatttgcct ggccctggac
     2281 atttcgcctt acccgtcttt tatatacccg gagctggata agcaacgaac gtcctggcct
     2341 gcttttcgat ctggcgacag ggtggcttat gcaacatcgt attattctcc ccggagccac
     2401 tacgctgacc cggttgattt cagaggtaag ggaaaaggcg acgttgcgcc tgtggaacaa
     2461 actggcactg ataccgtcag ccgaacagcg ttcacagctg gagatgctgc tggggccaac
     2521 tgattgcagc cgcctgtctt tactggaatc actgaaaaag ggccctgtga ccatcagtgg
     2581 tccggcgttt aatgaagcaa ttgaacgctg gaaaactctg aacgattttg gcctgcatgc
     2641 tgaaaacctg agtacactcc cggctgtgcg cctgaaaaat ctcgcacgtt atgctggtat
     2701 gacttcggtg
//
