LOCUS       NZ_CP079812             4982 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC02_S465MC plasmid
            pS465MC_p5, complete sequence.
ACCESSION   NZ_CP079812
VERSION     NZ_CP079812.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20115065
            Assembly: GCF_019334705.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC02_S465MC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC02_S465MC
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 91.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:44:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,688
            CDSs (total)                      :: 5,562
            Genes (coding)                    :: 5,417
            CDSs (with protein)               :: 5,417
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 61 of 145
            Pseudo Genes (incomplete)         :: 79 of 145
            Pseudo Genes (internal stop)      :: 24 of 145
            Pseudo Genes (multiple problems)  :: 17 of 145
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079812.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC02_S465MC"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pS465MC_p5"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2014"
                     /collected_by="Christian medical college"
     gene            join(4950..4982,1..135)
                     /locus_tag="KWL80_RS28790"
     CDS             join(4950..4982,1..135)
                     /locus_tag="KWL80_RS28790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_324169719.1"
                     /translation="MTNGAEKGCAARCAFSGNYHLESNPERHGSSPLAAAIGNGLEEVS
                     LEVLRQLRLN"
                     /name="replication initiation protein"
     gene            complement(288..908)
                     /locus_tag="KWL80_RS28230"
                     /old_locus_tag="KWL80_28205"
     CDS             complement(288..908)
                     /locus_tag="KWL80_RS28230"
                     /old_locus_tag="KWL80_28205"
                     /inference="COORDINATES: protein motif:HMM:NF025084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="WP_023312615.1"
                     /translation="MSKKHGNNKKVPQVMYIYCEGEKTEPLYLEAYISDRSKRTLSVFK
                     IPKTRKNTPEQLVDEAIKKKNSSSSAEGDVFWVVYDQEHLTPSSISCHQKAWNKANRHG
                     INIAISCVCFELWLLLHFGFTTKDFSSYDNLMSSSPLKKLITNYDKGSKLTYLAIRERI
                     GTAKDNARKLDIYAKTNYKNTHEPYLFPCYTSFHELLKAIDDF"
                     /name="RloB family protein"
     gene            complement(901..2256)
                     /locus_tag="KWL80_RS28235"
                     /old_locus_tag="KWL80_28210"
     CDS             complement(901..2256)
                     /locus_tag="KWL80_RS28235"
                     /old_locus_tag="KWL80_28210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498067.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_014386473.1"
                     /translation="MFVYLTIENFRSVKEKSTLNLSASGSSNHLTTHIYKNDGMNVGTL
                     MSAGIYGANASGKSNVLMAFEAIKFIATESGDLKEGKRIPCYEPYALSSATNEAPVKFE
                     AEFYSVEGNRFSYEVAFIKNRILFESLDYYPSRVKANLFTRDESDTWETIKFGGHYKGG
                     IKKIPFFPNNSYLAKAGNNAASPDIIKEAYNFFRKGIRHIGLNEKIRISSFGEREDVVN
                     ETAKVLCMVDTGVSGISVREVEAEIPIKMDEEIPQEIKEIIEEDYKYRYMFSHPTDEGG
                     SVSFPMGRESEGTQKLFEIIPLIRSAFNDSMVVIVDELDNSLHPHIADLIVKLFNDPDV
                     NKKGSQLVFSTHNMQLMAPEKMRRDQIWFCEKNKGASSLYSLDDFDKKKIKTTTPYAAW
                     YDEGRFGGVPDINYLKVASFISGDISLVMPDIDVKELSDGFFEEFDGDLSDE"
                     /name="ATP-binding protein"
     gene            complement(2889..3101)
                     /locus_tag="KWL80_RS28240"
                     /old_locus_tag="KWL80_28215"
     CDS             complement(2889..3101)
                     /locus_tag="KWL80_RS28240"
                     /old_locus_tag="KWL80_28215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3105..3590)
                     /locus_tag="KWL80_RS28245"
                     /old_locus_tag="KWL80_28220"
     CDS             complement(3105..3590)
                     /locus_tag="KWL80_RS28245"
                     /old_locus_tag="KWL80_28220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_013631087.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            complement(<3628..4305)
                     /locus_tag="KWL80_RS28795"
                     /old_locus_tag="KWL80_28225"
                     /pseudo=""
     CDS             complement(<3628..4305)
                     /locus_tag="KWL80_RS28795"
                     /old_locus_tag="KWL80_28225"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4268..4588)
                     /locus_tag="KWL80_RS28255"
                     /old_locus_tag="KWL80_28230"
     CDS             complement(4268..4588)
                     /locus_tag="KWL80_RS28255"
                     /old_locus_tag="KWL80_28230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_014386475.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(4703..4875)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.659"
                     /coverage="100.0"
                     /evalue="9.74e-41"
     Typing          complement(2774..4278)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.215"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgctgcgcct tctccggtaa ctatcatctt gagtccaacc cggaaagaca cggttcatcg
       61 ccactggcag cagccattgg taacggatta gaggaagtga gtcttgaagt tctgcgccag
      121 ttaaggctaa actgaaagga caatttttgg tgactgcgct ccctctaagc cagttacccg
      181 gtacataagt tggtagctca tgtaccttcc aaaaaccacc gttggtagcg gtggttttta
      241 tttttcatag attacagcct gtaataagcc atggtcaatg gattacttca aaagtcgtca
      301 atcgccttaa gtagctcatg gaatgatgta taacatggaa atagataagg ttcgtgcgtg
      361 tttttataat tagttttagc gtaaatatct agctttcttg cgttatcttt cgcagtacca
      421 atcctctctc ttatagcaag gtaagttagt ttactacctt tatcatagtt agttattaat
      481 ttttttagcg gggagctaga catcaaatta tcatacgacg aaaaatcttt ggttgtgaag
      541 ccgaaatgca acaataacca taattcaaaa caaacacagg atattgctat atttatccca
      601 tgcctattag ctttattcca tgccttttga tgacatgaaa tagacgaagg cgtcagatgc
      661 tcttgatcat aaacaaccca aaagacgtca ccttctgcgg aagatgatga attctttttc
      721 tttatcgcct catccaccaa ctgctcaggt gtatttttcc ttgttttagg tattttaaat
      781 acacttaaag ttcttttaga tctgtctgaa atataagcct caaggtataa tggttcggtt
      841 ttctcaccct cacagtatat atacattact tgtgggactt tcttattatt tccatgtttt
      901 ttactcatca gaaagatccc cgtcaaactc ttcaaaaaaa ccatcagaca attctttgac
      961 gtcaatatcc ggcattacca aggatatatc accagatata aatgaggcta cctttaagta
     1021 gtttatgtcg ggaaccccac caaacctacc ctcatcatac caagctgcgt aaggtgttgt
     1081 tgtttttatc tttttcttat caaaatcatc taagctataa agagacgacg cacctttatt
     1141 tttttcacaa aaccatatct gatctcttct cattttctct ggagccatta actgcatatt
     1201 atgggttgag aatacaagtt gtgagccttt tttattgaca tcaggatcat taaataactt
     1261 aacaatcaag tcagcaatat gcgggtgcaa gctattatct aactcatcaa caattacaac
     1321 catactatca ttaaacgcag atctaattag aggtatgatt tcaaataatt tttgggtgcc
     1381 ttcagactct ctccccatag ggaaagaaac actcccccct tcatcagtag gatgagagaa
     1441 catataacga tatttataat cttcttcaat tatctccttt atttcttgag ggatttcctc
     1501 atccattttt attggtattt ccgcctcaac ctcccgtaca gatataccgg aaacaccagt
     1561 gtcaaccatg cataaaacct tggccgtttc atttacgacg tcctccctct ccccaaaaga
     1621 agatattctt atcttttcat ttaaacctat atgtctaatt ccctttctaa aaaagttata
     1681 agcttcttta attatgtctg gtgatgcggc attattacct gctttcgcta agtatgaatt
     1741 attagggaag aatggtattt ttttaatacc tcctttataa tgcccaccaa acttgattgt
     1801 ttcccaggtg tcgctctcat ctctagtaaa taaatttgct ttaaccctag atggataata
     1861 gtctaatgat tcaaataaaa tccgattttt aataaaagca acttcataac tgaaccgatt
     1921 cccctcaaca gaataaaatt cagcctcaaa ctttacagga gcctcattcg tagccgaaga
     1981 aagagcatat ggctcatagc agggaatccg tttaccttct ttcaaatcac cgctttctgt
     2041 tgcgataaat tttatagctt caaaagccat gagaacgtta gatttaccag acgcattagc
     2101 accataaatc ccagcactca tgagcgttcc aacattcatt ccatcatttt tatagatatg
     2161 agttgttaaa tggttactag aaccggatgc gctcaagttt aatgtcgatt tttccttaac
     2221 cgacctaaaa ttttcaatag tcaaataaac aaacatgcat acctcctaac ggggattttt
     2281 cgccaaaaaa gcgaaaaatc aatcatagga aagatatcct atatgtttta attttggaac
     2341 aacatcttcc ttaaaaaacg ttgtgctatt tataattaca cgctatttac cataacatct
     2401 gttgcccgca ccgctccgga caggttcgcc acaaacgggg cggcagtcag gtaaaaccgg
     2461 caacaaacca cccgatacac cgccccgttc gtgacagata aattttaccc ggctgcatgg
     2521 tcatgcagcc agatgcatac ggtctgattt caggccagaa tccggatgaa cgccttaaaa
     2581 acaggaaaat gcttctccag ccagtcccag atctccagcg ccatgtacag gcaattcagc
     2641 accgtctcaa tcacgttaaa cagtctcata atccctccgt tgtatacgct gtgatggaaa
     2701 catcacgacc gtatttagcg ggaaatttat gagcccttct tcttggggtt tgtcactgga
     2761 tgcgcgcagt cagcccagcg tcggaccgtc atagctccgc tgccgggaac gggtttccgc
     2821 cagctctttt tcatgctgtt tctgctcgta tgcccgttcg gcggccaccg ccatttcatg
     2881 ccgctgcact actggctcaa acgctgtaac tgcctgctca agttcgccac ctgctgactc
     2941 aagcgcgtga cgcgctcgtt cagcgccgcg ttctcccgtt gcataagacc agacatcttc
     3001 tgccattccg cgaaggcgct ctcccactcg tccagccttt gcgagtagtc ctgatgtagc
     3061 tgctctaatg cggtcagcaa ctgtttttcc agttccgtca tgggctattt cctctccagt
     3121 accatccagt tcccttcctc cccgtaagct cccgctttcg ggttcacttt gacgcaggac
     3181 agtttcttct ccccgcagag cgacagctgc acgccgtggt tcttctccga cagcatggcc
     3241 tgcgtccgct cctgcgcccg gatagtctgg taattcccgg tgatctgctg cccctgccac
     3301 cacagaatgc tcccgctcgt ggcgatcagc agtaccgaga ccagggtgat ggtcagccac
     3361 gtccggccga ccatacgcag cacgctcagc cggttcgact gcatcgccgc ggtcataccc
     3421 tgctcgtggg cgctgatggc gtcgctgatt ctcttcgcgc ttgcgctcag ttccgctttt
     3481 acagactgct cgtgctcgct gaatgcggct ttcagcatct cgccggtact ctgctgctgc
     3541 gctttcgatt gctgctccaa gtccttcgcc agcgctaaaa gactgctcat aaatggctcc
     3601 cttcagtcgg atgtttcgcc ccccgtccgg gtcggcaatg ctgatactgt tttttgtggt
     3661 gcggaccacc tcaaaacctg ccgctgtgag cgcccctgtg acgtcctcgc gcgttttgag
     3721 ctccccggat gaggctaagg ccagcaaccc acgtgtaatc gcctgagcgg cttccagctt
     3781 cgtttccggc agtgcggacg gcgtgaccag taaccgtcgg ttctccgggg cgtttggatc
     3841 atgcagcccc agcctgccgt tcaccacggt ctgccaggca tcgatgcgcg gccggtcggc
     3901 gcgatcgtag tacggctgca ggcgtttccc ggtcagcagt tcagtgttgg ggatcaggaa
     3961 attcagctcc agccgcccct tgtcggtgtg ctccacccac agaatgctgt actggtcttt
     4021 atcgagcccg ggcatcagaa cccgctcaaa gctggccatg atctgctccc gctgcccggg
     4081 cggcagttca gcctccgcga acgacagcac gccggaggtg tactttttcg catacggcga
     4141 ggcatcgata agctcccgga cttcctccgg ttttccctgc agcacggtcg ccccctcgcg
     4201 ctgccggtct tttcccagca ggtaatccac cggaccggca ccaccaccgc gcccccgggg
     4261 gtgaaattta acgatcatca tccccccctt tttccagcac cgcatgccgc agccgctcca
     4321 gtcccgcgtc gattgccatc agtgcggcca caatctgcac ccggtcatga ccggcaccac
     4381 cggcattcac tttccgggcg atctggttca ggttgttccc cataccggca agctggcgca
     4441 gcagcgccgg cgatatcgac ggcagtctgc cggcacgcgc cggcttctca tcgaggcagg
     4501 tctgacgcat ccacgccgcc agctgcttgc cgtcgcaccg ctcgagcagc cgccggtgtt
     4561 cgtcttccgt cacccacatc gtgagcatct tgttgcgttt gtctgccaga gtagcctcct
     4621 gataacaccg ggacgggcgg gaaatgtttc ccggtcggca cggtgttgga caagccgcag
     4681 gcgcgtcagt cgcttttagc gggtttcggg gcgcagccct gaaccagtca cgtagcgcta
     4741 gcggagtgta tactggctta ttatgttggc atgagtgcgg taatcaggaa agtgcttcat
     4801 gtgacagagg aaaatgcagc tgcgggcgta aggcagaaaa tggaagttca gaggatatga
     4861 cgcctcctcg ctcactcagt cgctgccgct cctgccgttc ggctgcggcg ggcgttacct
     4921 gcacacggag aagagcgaag gcatatggca tgacaaacgg ggcagaaaaa ggatgcgccg
     4981 ct
//
