LOCUS       NZ_CP079734             3559 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP3295 plasmid pKp3295-9, complete
            sequence.
ACCESSION   NZ_CP079734
VERSION     NZ_CP079734.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20170224
            Assembly: GCF_019334545.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Wu,Y.
  TITLE     The genome of KP3295
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Wu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUL-2021) Clinical Laboratory, Sir Run Run Shaw
            Hospital, Zhejiang University School of Medicine, Caihe, Hangzhou,
            Zhejiang 310000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:24:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,654
            CDSs (total)                      :: 5,534
            Genes (coding)                    :: 5,354
            CDSs (with protein)               :: 5,354
            Genes (RNA)                       :: 120
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 180
            CDSs (without protein)            :: 180
            Pseudo Genes (ambiguous residues) :: 0 of 180
            Pseudo Genes (frameshifted)       :: 71 of 180
            Pseudo Genes (incomplete)         :: 112 of 180
            Pseudo Genes (internal stop)      :: 31 of 180
            Pseudo Genes (multiple problems)  :: 31 of 180
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079734.1.
            Bacteria and source DNA available from The Fourth Affiliated
            Hospital of Zhejiang University School of Medicine.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP3295"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKp3295-9"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="29.30 N 120.07 E"
                     /collection_date="2018-08-21"
                     /collected_by="The Fourth Affiliated Hospital of Zhejiang
                     University School of Medicine"
     gene            737..961
                     /locus_tag="KXI30_RS28155"
                     /old_locus_tag="KXI30_28145"
     CDS             737..961
                     /locus_tag="KXI30_RS28155"
                     /old_locus_tag="KXI30_28145"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071653266.1"
                     /translation="MSNKDSLERKVTPRKNEPVFPMALRVGIAEDICFLELVDFPEKES
                     GRVFYSIALTKKHAKNLIDGLNKFVESGD"
                     /name="hypothetical protein"
     gene            963..1607
                     /locus_tag="KXI30_RS28160"
                     /old_locus_tag="KXI30_28150"
     CDS             963..1607
                     /locus_tag="KXI30_RS28160"
                     /old_locus_tag="KXI30_28150"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044816498.1"
                     /translation="MKYINHCDSEDYYITNDSIDLGVRYDVSDYNKLELNDLRDPDLFK
                     VLGRAFVVVENSVWESWNDDVKTLDCSGDGYESILKHYESLELSGEFLLFFKKIRSAIS
                     VYNREYEFCTGDLEPSLQAIVQLIRYAPELAAKKDAQIYFDKKSECFGLIYKQTKKSKG
                     TLNLIVKTNNEIIFSFVRKVSGVVKVTGRAYIGDSLDNSIAIKSLFKMMEW"
                     /name="hypothetical protein"
     gene            1607..2083
                     /locus_tag="KXI30_RS28165"
                     /old_locus_tag="KXI30_28155"
     CDS             1607..2083
                     /locus_tag="KXI30_RS28165"
                     /old_locus_tag="KXI30_28155"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_077861907.1"
                     /translation="MTVLSTLNNLKSIPCINAFTGRANNSYLWRYVMEDHKDEFGATTI
                     KCFYLREEISEDYVSIYHSNKDYYEGMVRDVHKRIPVGKTKPAIYLSFDAAEVIEQVNK
                     PDKCIDIKDEGYPHLGLWYLFDINDEFTLIEVTTTILELSSFFQRNEGGVVVPL"
                     /name="hypothetical protein"
     gene            complement(2290..2403)
                     /locus_tag="KXI30_RS28415"
     CDS             complement(2290..2403)
                     /locus_tag="KXI30_RS28415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071653267.1"
                     /translation="MHEPPVQSDCCAFSGNYQLESNPERHDEAPLAAATGN"
                     /name="replication initiation protein"
     gene            2819..3010
                     /locus_tag="KXI30_RS28170"
                     /old_locus_tag="KXI30_28160"
     CDS             2819..3010
                     /locus_tag="KXI30_RS28170"
                     /old_locus_tag="KXI30_28160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_044816495.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSGLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3010..3432
                     /locus_tag="KXI30_RS28175"
                     /old_locus_tag="KXI30_28165"
     CDS             3010..3432
                     /locus_tag="KXI30_RS28175"
                     /old_locus_tag="KXI30_28165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_044816494.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKNTKVVD
                     TSELIRVFGSLYGGGDTVKHDVKGDAGATGVNISGLHDLQHKIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKLEPSPQVHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(2291..2698)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.39"
                     /coverage="99.0"
                     /evalue="1.26e-177"
ORIGIN
        1 gcagatttgt attttatatc agtagtttac gttctataaa tttcgttttt ttaattttat
       61 ttatacagat ccgactctat gacgactcgt catagagtcc ccggcgagtc gtcatagagt
      121 cggtaacgac tcaggactat aaaaacaata agttaacaac gacccgactc tagacccgac
      181 tccccgtttg gttgtttttt agacgtgacg actctgggta cgactctagt gaagagtcgc
      241 cggtgaatcg tgtggtgagt cgtccggcga cttgccataa cgtgattgcc ggccgtcgac
      301 gaacccgatt cctgaacgtg ctgaaaaccg gttttccggc tgcgccggca acgcttcacg
      361 gccacgcaat aaattgcgtc cccgttcacc gttttcggat gggaagaacg ccggaaacag
      421 gacttaccct ggctgaatgg cgtgaatttt cgcgctatgc acttgcgtgc atactcatgc
      481 atgccgtaaa aacagagcct gcgcgtttct ggcgggtttt cgggtggttt gttgcctatt
      541 ttaccggttt cccgtcagaa acgccctgag gccgttttag cggtgcgtac aattttggtg
      601 ttatgttaaa tcatggccac aaaattccat aaagtgacca atgtaatgtt aaatgagcat
      661 tgtggatgaa agatgttgtt ttatggtatt attttttatg ttctaaactt aaatttattt
      721 ctatcggggt attataatga gcaataaaga ttctttagaa agaaaagtaa caccaagaaa
      781 aaatgaacct gtattcccga tggctctaag agttgggata gctgaagata tttgtttttt
      841 agagttagtg gactttcctg aaaaagaatc aggaagggtt ttttattcca tagctctaac
      901 caaaaaacat gcaaagaacc ttatcgatgg attgaataaa ttcgttgagt caggtgatta
      961 gtatgaaata cattaaccac tgcgatagtg aagattatta tattactaat gatagcattg
     1021 atctcggtgt tcgttatgat gtttctgatt ataataagtt agagctaaat gatttaagag
     1081 atccggattt gtttaaagtt ttgggtcgag catttgttgt tgttgagaat tctgtttggg
     1141 agtcttggaa tgatgatgtt aaaacattag attgctctgg tgacggttat gagtcaattt
     1201 taaaacatta cgaatcatta gaattatcag gtgaattctt attgtttttt aaaaaaataa
     1261 gaagtgccat ttctgtttat aatagagaat acgagttctg cactggtgac ctagaaccat
     1321 ctttgcaggc tattgttcag ttgattaggt atgcccctga acttgctgca aaaaaagacg
     1381 ctcagattta ctttgataaa aaatcagagt gttttgggct tatatataaa caaactaaga
     1441 aaagcaaagg cacgctaaat ttaatagtga aaacaaataa cgagattatt ttttcctttg
     1501 ttagaaaagt ttctggagtg gttaaggtta caggtagggc atatattggc gatagccttg
     1561 ataactccat tgctatcaaa tcattattta aaatgatgga gtggtgatga ctgtgttatc
     1621 aactttaaat aacctcaaat caatcccttg tattaatgca tttacgggca gagcaaataa
     1681 ttcatattta tggcgttatg ttatggaaga tcataaagat gagtttggcg ctacaactat
     1741 taagtgtttt tatcttaggg aggaaatttc agaggattat gtgtctattt atcattcaaa
     1801 taaagattat tatgaaggaa tggtaaggga tgtacataaa agaatccctg tagggaaaac
     1861 caaacctgcc atatatctct catttgatgc agcggaagtg atcgagcaag ttaacaaacc
     1921 agataaatgt attgatataa aggacgaagg ttaccctcac ttgggtttgt ggtatttatt
     1981 tgatattaat gatgaattta ctttgattga ggtgactaca actattttgg aattatcctc
     2041 tttttttcaa agaaatgaag gtggcgtggt tgttcctctt tagcgaaaac cgtcttgacg
     2101 tcatcaagtg tgacctagta ctcttcttga aaaagaaaaa ccgcctttgc ggggcggttt
     2161 ttcttaggtt ctaagagttt gcggctctat gaaccaaggt aactggctag ttagaggagc
     2221 gcagtaacca aaatcgttct ttcagtttag ccttaactgg cacataactt caagactaac
     2281 tcctctaaat cagttaccag tggctgctgc cagtggcgct tcgtcgtgtc tttccgggtt
     2341 ggactcaagt tgatagttac cggaaaaggc gcagcagtcg gactgaacgg ggggttcgtg
     2401 catacagtcc agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaatgag
     2461 ataaacgcgg ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc
     2521 gcacgaggga gccaccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2581 accactgatt tgagcgtcag atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa
     2641 acggccgctg tgcggcctct ccttctcccc atcacgcgat cgctcggttt tctcgagctt
     2701 ttatacgcat gctcctgccg ctcgccgcag ccgaacggca ggagcgtagc gactgagtga
     2761 gcgaggaagc ggaaaagaga acatccctac actgagcact gacgttctga ggcctcttat
     2821 gacgaaacaa cagaaaacag cgctgaacat ggctaaattc atccagaatc agtctctgct
     2881 cctgctggag aagcttaacg agcttgatct tgatgtagag gccgaccttt gtgagaaact
     2941 ccacgatgat gcggagcatc ttttccgtac actgtcttcg ggactggatg aattacagga
     3001 tggaaactga tggccagagt gtctattagt gaagcggctc ggcttgttaa ggttagtcgc
     3061 cccacaattt acaagatgat taacagtggg aaactaagtt acacctctgt tgtaaagcat
     3121 gggaaaaata ctaaagttgt tgatacatca gagcttatta gggtttttgg ctcgctttat
     3181 ggtgggggcg acactgtaaa acatgatgta aaaggtgacg caggcgctac aggggttaac
     3241 atctctggtt tacatgattt acagcataaa attgctttgc ttgaagctga aaacgatggt
     3301 cttaaagggg ctgttaaagc tcgtgatgaa catatcgatt ctcttcgcca ggctatgcag
     3361 cttctggaac acaagctcga accctctcca caggttcatt ctccgtggtg gaaattctgg
     3421 aagaaatcct gaccccgtca tgcttcagtt ttcctctccg gccattccgg ggagttcctg
     3481 aaccttccca aaaatcgcca gatctctgaa cgctcctcac cactaaaaat atccacaccc
     3541 cacgcaaaat taaaatttt
//
