LOCUS       NZ_CP079733             3770 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP3295 plasmid pKP3295-8, complete
            sequence.
ACCESSION   NZ_CP079733
VERSION     NZ_CP079733.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20170224
            Assembly: GCF_019334545.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Wu,Y.
  TITLE     The genome of KP3295
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Wu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUL-2021) Clinical Laboratory, Sir Run Run Shaw
            Hospital, Zhejiang University School of Medicine, Caihe, Hangzhou,
            Zhejiang 310000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:24:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,654
            CDSs (total)                      :: 5,534
            Genes (coding)                    :: 5,354
            CDSs (with protein)               :: 5,354
            Genes (RNA)                       :: 120
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 180
            CDSs (without protein)            :: 180
            Pseudo Genes (ambiguous residues) :: 0 of 180
            Pseudo Genes (frameshifted)       :: 71 of 180
            Pseudo Genes (incomplete)         :: 112 of 180
            Pseudo Genes (internal stop)      :: 31 of 180
            Pseudo Genes (multiple problems)  :: 31 of 180
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079733.1.
            Bacteria and source DNA available from The Fourth Affiliated
            Hospital of Zhejiang University School of Medicine.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP3295"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP3295-8"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="29.30 N 120.07 E"
                     /collection_date="2018-08-21"
                     /collected_by="The Fourth Affiliated Hospital of Zhejiang
                     University School of Medicine"
     gene            join(3391..3770,1..619)
                     /locus_tag="KXI30_RS28135"
                     /old_locus_tag="KXI30_28125"
     CDS             join(3391..3770,1..619)
                     /locus_tag="KXI30_RS28135"
                     /old_locus_tag="KXI30_28125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="WP_001477600.1"
                     /translation="MGHGVTVLFLLLLLVHIIFDFYLQTEEMVREKAFDNGIGRALKAH
                     LFHAVMHGFFLCLSCFAFIYVLLGCTNLLNFMLFVLLFSLFTFFHFAIDVAKSITSLKV
                     KGFYCFAVFLFDQVLHILTIFFVIYYFFCDYGVYIYDFNFIKYVGGVLIFACSIAFLLK
                     PSSIIVYLFFKSSGIGDGSEKVKITKSKIAELLYIVLNKELGDSDVKDCKVAVNKYKKK
                     AEEIVIGISDGQADIDIRTISKVNNAGRFIGYLERLIIFLFLILGSVTAVVAVLAMKTA
                     LRFSDLKDDNDSGKAEYIMIGTFFSLIVTVIISVLARMYLIEIGIKINDFS"
                     /name="DUF3307 domain-containing protein"
     gene            complement(887..1036)
                     /locus_tag="KXI30_RS28530"
     CDS             complement(887..1036)
                     /locus_tag="KXI30_RS28530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_162847635.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPGRHDKAPLAAAIDKSRCARDFR
                     ECLK"
                     /name="replication initiation protein"
     gene            complement(1523..1717)
                     /locus_tag="KXI30_RS28140"
                     /old_locus_tag="KXI30_28130"
     CDS             complement(1523..1717)
                     /locus_tag="KXI30_RS28140"
                     /old_locus_tag="KXI30_28130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041619.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEAENRTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2001..2324
                     /gene="mobA"
                     /locus_tag="KXI30_RS28145"
                     /old_locus_tag="KXI30_28135"
     CDS             2001..2324
                     /gene="mobA"
                     /locus_tag="KXI30_RS28145"
                     /old_locus_tag="KXI30_28135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_052522663.1"
                     /translation="MTKRSGSNTRRRAICRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            2712..3380
                     /locus_tag="KXI30_RS28150"
                     /old_locus_tag="KXI30_28140"
     CDS             2712..3380
                     /locus_tag="KXI30_RS28150"
                     /old_locus_tag="KXI30_28140"
                     /inference="COORDINATES: protein motif:HMM:NF016205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015059034.1"
                     /translation="MSDSGLMMGVITGDLYRSSTDYERGVSYIGLMEVMLEKIKKNNRL
                     RVSDVDFFRGDSFQITSKDPINLLSIAVYIRAYLRSYSEKNDGKGMYDARLAISIDKVN
                     KYRRDINSIFESAQIKSGKSLDLIENDEMMVFDSSLYDLKEIYSPSIILLDALLSMLSK
                     PQSEILREIVYDRDASISEIAERLGKSKQVVYKTIKRGAVDKIIKFLDAHLSLLGMRFF
                     "
                     /name="hypothetical protein"
     Typing          1753..1992
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.585"
                     /coverage="100.0"
                     /evalue="3.54e-121"
     Typing          complement(965..1488)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.528"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          2002..2321
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.131"
                     /coverage="100.0"
                     /evalue="1.44e-61"
ORIGIN
        1 tatttattat ttcttttgtg attatggcgt atatatatat gattttaatt tcataaaata
       61 tgttggcggg gtgttgatat ttgcttgttc tatagcgttt ttactaaagc cttcatctat
      121 tatagtttac ttattcttta aatcatcagg tattggtgat ggttcagaga aggttaaaat
      181 aaccaaaagt aaaattgcgg aattgcttta tattgtattg aataaagaac tgggtgattc
      241 tgatgttaaa gattgcaagg tggcagtaaa taaatacaag aaaaaagcag aggaaattgt
      301 aatcggtatt tctgatgggc aagctgatat tgatatcaga actatttcca aagttaataa
      361 tgctggtaga tttataggtt accttgaaag attgataatt tttttgtttt tgattttagg
      421 ctcggtcact gcggttgtcg cggtgttagc aatgaaaaca gctctgagat ttagtgatct
      481 aaaagatgat aatgatagtg ggaaggctga atatataatg attggtacat tttttagtct
      541 tattgtaacc gtgataatat ctgtgttagc cagaatgtac ttgattgaga ttggtataaa
      601 aattaatgat tttagttagt ttgagctcag tttctcacat aagaagatga tggttgcaat
      661 gatgagaaat gatatcaatg gaatgatgat gcattttcgt atctgagaag gatcttcttg
      721 agatcattta tatctgcgcg taatctcttg cactggaaac gaaaaaacca cctggggagg
      781 tggtttttcg aaggttaagt cagttgggga actgcttaac caggtaactg tcttgtgcga
      841 gacgagccac caaaactgtt cttttagtgt agctgtagtc cggccatcac ttcaagcact
      901 ctctaaaatc tctcgcacat cgactcttat caatggctgc tgccagtggc gctttgtcgt
      961 gtcttcccgg attggactca agtcgatagt taccggataa ggcgcagcgg tcgggctgaa
     1021 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     1081 aacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     1141 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     1201 ggtatcttta agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1261 ctcgtcaggg gggcggagcc tatggaaaaa cgtctgcggc gctggcttct tccggtgctt
     1321 tgctttttgc tcacatgttc tttccggctt tatcccctga ttctgtggat aaccgtatta
     1381 ccgcttttga gtgagctgac accgctcgcc gcagtcgaac gaccgagcgt agcgagtcag
     1441 tgagcgagga agcggaagag cgccttatgt gacattttct ccttacgctc ctctatgccg
     1501 ttctgcatcc tgtccggatg cgttatatcc cggtgcgatt ttccgcttca aagcgtgtct
     1561 gtatgctgtt ctgaagttct tccgcgagtt cgtgcagctt ctcacacatg gcggcctgtt
     1621 cgtcagcatc gagtgcgtcc agtttttcga gcagcgtcag gctctggctt tttatgaatc
     1681 ctgccatgtt gagtacggct tgctgctgct tgttcatctt ttcgttttct ccgttctgtc
     1741 tgtcatctgc gttgtgtgat tatatcgcgt accacttttc gactgtttta ccgccggtat
     1801 tctgccgtct gacgctttga cgggtatttc tgcctgacaa tactgtcact gccaaaaaac
     1861 tgccgtgtct ttgtcggtaa ttcgaacttg ctgacaggac aggatgtgta attgttatac
     1921 cgcgcatgca tgcacgctat tacaattacc ctggtcaggg ctttgccccg acacccatgt
     1981 cagatacgga gccatgtttt atgacaaaac gaagtggaag taatacgcgc aggcgggcta
     2041 tctgtcgccc tgttcgtcta acggcagaag aagaccagga aatcagaaaa agggctgctg
     2101 aatgcggcaa gaccgtttcc ggttttttac gggcggcagc tctcggtaag aaagttaact
     2161 cactgactga tgatcgggta ctgaaagaag ttatgagact gggggcgtta cagaaaaaac
     2221 tctttatcga cggcaagcgt gtcggggaca gggagtatgc ggaggtgctg atcgctatta
     2281 cggagtatca ccgtgccctg ttatccaggc ttatggcaga ttagcttccc ggagagaaaa
     2341 ctgtcgaaaa cagacggtat gaacaccgta agctcccaaa gtgatcacca ttcgctttca
     2401 tgcatagcta tgcagcgagc tgaaaacgat cctgacgcat ccttcctgtt ttcccggggt
     2461 aaaacatctc tttttgcggt gtctgcgtca gaatcgcgtt cagcgcgttt cagcggtgcg
     2521 tacaattatg gctttatgtt aaatgcaggg tgacaactga attttaatat tgagcgcctt
     2581 agagccaaga agtatcaagt tattggtgta aaccaaaatg ggttactttt ttgtttttca
     2641 acctttttgg ttgactattt tccttttcgg ttgtgcaatc tgtcaagaat tattttagtt
     2701 tgggggatga aatgagtgat tcagggttaa tgatgggggt tatcactggt gatttgtacc
     2761 gctcgagtac cgattacgaa cgaggggttt cttacattgg cttgatggag gttatgttag
     2821 aaaagataaa gaaaaacaat aggttaagag ttagtgatgt tgattttttt cgaggggatt
     2881 catttcagat aaccagcaaa gatcctatta atttattaag tattgccgtt tatattaggg
     2941 cttatttaag atcatatagt gagaaaaacg atggtaaagg gatgtatgat gctcgcttgg
     3001 cgatatccat agataaagtt aataagtata gaagggatat aaactcaata tttgaatctg
     3061 ctcagataaa gtcagggaaa agtttagatc tcattgaaaa tgatgagatg atggtcttcg
     3121 actcatcact ttatgattta aaggaaatat attccccatc tattattctt cttgatgcat
     3181 tgctttcaat gttgtccaaa cctcaatcag aaatcctaag ggagattgta tatgataggg
     3241 atgcaagtat ttctgaaata gcagaaagac tgggaaagag taaacaagtt gtttataaaa
     3301 ccataaaaag aggagcggtt gataagataa taaagttttt ggatgctcat ctttcactgc
     3361 ttggaatgag atttttttaa tggagggggt atggggcacg gtgtaactgt tctatttttg
     3421 ttgttgcttt tggttcatat aatttttgac ttttatttgc aaactgagga gatggttaga
     3481 gaaaaggcat tcgataatgg cattggtagg gctttaaagg ctcacttatt tcatgccgtg
     3541 atgcatggtt tttttctatg tctatcctgc ttcgctttca tatatgtact gcttggctgt
     3601 accaatctat taaactttat gctatttgtt ttgctgtttt ctttatttac attttttcat
     3661 tttgcaatag atgtcgcaaa gtcgattacc tctctgaaag taaagggttt ttattgtttt
     3721 gctgttttct tgtttgatca agttttgcat attttaacta tattttttgt
//
