LOCUS       NZ_CP079732             4510 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP3295 plasmid pKP3295-7, complete
            sequence.
ACCESSION   NZ_CP079732
VERSION     NZ_CP079732.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20170224
            Assembly: GCF_019334545.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Wu,Y.
  TITLE     The genome of KP3295
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Wu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUL-2021) Clinical Laboratory, Sir Run Run Shaw
            Hospital, Zhejiang University School of Medicine, Caihe, Hangzhou,
            Zhejiang 310000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:24:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,654
            CDSs (total)                      :: 5,534
            Genes (coding)                    :: 5,354
            CDSs (with protein)               :: 5,354
            Genes (RNA)                       :: 120
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 180
            CDSs (without protein)            :: 180
            Pseudo Genes (ambiguous residues) :: 0 of 180
            Pseudo Genes (frameshifted)       :: 71 of 180
            Pseudo Genes (incomplete)         :: 112 of 180
            Pseudo Genes (internal stop)      :: 31 of 180
            Pseudo Genes (multiple problems)  :: 31 of 180
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079732.1.
            Bacteria and source DNA available from The Fourth Affiliated
            Hospital of Zhejiang University School of Medicine.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP3295"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP3295-7"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="29.30 N 120.07 E"
                     /collection_date="2018-08-21"
                     /collected_by="The Fourth Affiliated Hospital of Zhejiang
                     University School of Medicine"
     gene            join(4448..4510,1..294)
                     /locus_tag="KXI30_RS28105"
                     /old_locus_tag="KXI30_28095"
     CDS             join(4448..4510,1..294)
                     /locus_tag="KXI30_RS28105"
                     /old_locus_tag="KXI30_28095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_032413514.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIERVTPAHEIIER
                     KHNAESLEKKHAVA"
                     /name="HigA family addiction module antitoxin"
     gene            1123..1314
                     /locus_tag="KXI30_RS28110"
                     /old_locus_tag="KXI30_28100"
     CDS             1123..1314
                     /locus_tag="KXI30_RS28110"
                     /old_locus_tag="KXI30_28100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032421723.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1314..1724
                     /locus_tag="KXI30_RS28115"
                     /old_locus_tag="KXI30_28105"
     CDS             1314..1724
                     /locus_tag="KXI30_RS28115"
                     /old_locus_tag="KXI30_28105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_060578860.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKIIKVID
                     TSELIRVFGSLDGVIDTVKSDAGSTGVNSVGLHDLQHRIALLEAENDGLKGAVKARDEH
                     IDSLRQAMQLLEHKHEPSSPPDSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            2511..2957
                     /locus_tag="KXI30_RS28120"
                     /old_locus_tag="KXI30_28110"
     CDS             2511..2957
                     /locus_tag="KXI30_RS28120"
                     /old_locus_tag="KXI30_28110"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_125450400.1"
                     /translation="MNDKSGFSFMKIGAGAKVTGLRMKRNVMVVDDLSHVESFVTVGER
                     GEVINLDADGNELHTPDSYREKLKIERGELQASLEKAKEEINSIDNDITSQKIKNQLSA
                     ALSQPMDAEGEWNTKRILQGVLDLSKQVGCGVLTAAITHYLGVS"
                     /name="hypothetical protein"
     gene            complement(3499..3741)
                     /locus_tag="KXI30_RS28125"
                     /old_locus_tag="KXI30_28115"
     CDS             complement(3499..3741)
                     /locus_tag="KXI30_RS28125"
                     /old_locus_tag="KXI30_28115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_071993219.1"
                     /translation="MALVRSGNKTKIFEADPKFNPVFDTQHKPGDTCTYEGIYKCTGCG
                     KEVTIAGGKTLPPQNHHQHSPGQGDIRWQLFVYAE"
                     /name="protein L"
     gene            3924..4244
                     /locus_tag="KXI30_RS28130"
                     /old_locus_tag="KXI30_28120"
     CDS             3924..4244
                     /locus_tag="KXI30_RS28130"
                     /old_locus_tag="KXI30_28120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_072198282.1"
                     /translation="MQQNHDSDDDCRIQFRDDWLYQFYKYGKANRKIPATISVSLGRKL
                     DMLNAADKIGDLRSPPGNRFENLNPPLTGYSSIRVNDKYRLIFKWGENGVEDLYLDPHD
                     YR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          complement(595..1004)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccaagtggtt taaaaatcaa tgacttggct gaaatgttaa acgtgcatag aaattctgca
       61 agtgctttgg ttaacaataa taggaaactg tctaccgata tggcgtttcg gttagctaaa
      121 gcattcgata ctaccgctaa gttctggtta aatatacagc agtctgttga tttgtgggag
      181 gttacgagca gcccacgcct tcaggaagaa atagaaagag tcactcccgc tcatgaaatt
      241 atagaaagga agcacaatgc tgaatctttg gaaaaaaaac atgcggtcgc atgatttttt
      301 taaaaaaatt taaccaaact agaagaaggc ctctgtaata gtgtgccttc tttttttgta
      361 accgctaaag gtcatcatca ttttctttta cgagcacttg agatccggtt tcggtacgcg
      421 taatctcttg ctctgtaaac ggaaaaaccg ccttgcaggg cggtttttcc gaaggttcaa
      481 acacttgcag gtgattgaac caaggtaact ggcttggagg agcgcagtca ccaaaacttg
      541 ttctttcagt ttagccttaa caggcgcatg acttcaagac taactcctct aaatcagtta
      601 ccagtggctg ctgccagtgg cgcttttgca tgtcttaccg ggttggactc aagacgatag
      661 ttaccggata agacgcagca gtcggactga acggggggtt cgtgcataca gtccagcttg
      721 gagcgaactg ccttcccgga actgagtgtc tggcgtggaa tgagataaac gcggccataa
      781 cagcggaatg acaccggtaa accgaatggc aggaacagga gagcgcacga gggagccatc
      841 agggggaaac gcctgatatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg
      901 tcaatttcta tgatgcttgt caggggggcg gagcctatgg aaaaacggcc gctaagcggc
      961 ctttccttct gcccatcacg cgtccgctcg gttttctcga gcatttatac gcatagtcct
     1021 accgctcgcc gcagccgaac ggcaggagcg tagcgactga gtgagcgagg aagcggaaaa
     1081 gagactggtt ttacactgag cactgacgct ctgaggcttc ttatgacgaa acaacagaaa
     1141 acagcgctga acatggctaa attcatccag aatcagtctc tgctcctgct ggagaagctt
     1201 aacgagcttg atcttgatgc agaggccgac ctttgtgaga aactccacga tgatgcggag
     1261 catcttttcc gtacactgtc ctcgagactg gatgaattac aggatggtaa ctgatggcca
     1321 gagtgtctat tagtgaagcg gcacggcttg ttaaggttag tcgccccaca atttacaaga
     1381 tgattaacag tgggaagtta agttacacct ctgttgtaaa gcacgggaag atcattaaag
     1441 ttattgatac ttcagagctt attagagttt ttggttctct tgatggtgtc atcgatactg
     1501 taaaaagtga cgcaggatct acaggtgtta acagcgttgg tttacatgat ttacaacata
     1561 gaattgcttt actagaagct gaaaacgatg gtcttaaagg ggctgttaag gctcgagatg
     1621 agcatattga ttcccttcgt caggctatgc agcttctgga gcacaagcac gaaccttctt
     1681 caccacccga ttccccgtgg tggaaattct ggaaaaaatc ctgactccgt catgcttccg
     1741 ttttcctctc cggccatccc ggggagttcc tgaaccttcc caaaaatcgc cagatctctg
     1801 aacgctcctc accactaaaa atacccacac cccacgcaaa attaaaattt tgaagataat
     1861 tcattaatat cagttagttg tgggttatta cattacgcag tattactttc tgtaaggtga
     1921 atccaagtcg tcggcgattc gtgtacgact tgccgacgac tcgtcataga gtcatcgacg
     1981 actcacttaa gactatcagt aacttatgac gacttgactc tagagtcgaa tcgtcatttt
     2041 ggtgtttttt tgaccttggc gactctagag tcaagtcacg agtcgaatcg ccggtgaatc
     2101 gtgtggtgag tcgtccggcg acttgcataa cgtgattgcc ggccgtcgac gaacccgatt
     2161 cctgaacgtg ctgaaaaccg tttttccggc tgcgccggca acgcttcacg gccacgcaat
     2221 aaattgcgtc cccgttcacc gttttcagtc tggtttggtg cgggttttga gttgtctggc
     2281 ctgaattcag gctgaaatcc gccaccagtc agtctggtgc ataacaattc atgcccgtaa
     2341 aacgccctgg agcgattttg aggcgttttc agggggtaag acgtaggatt taatggatta
     2401 cgccccagaa tcgtttctag agcgtttgag cggtgcgcgt aatgaggcgt tatggtaaat
     2461 aattgctcgt gaaaacgtta ttaacatttt tatcttatga ggtgatgtga gtgaacgata
     2521 aatcaggctt tagttttatg aagatcggtg caggtgcaaa agtgactggt ttaagaatga
     2581 aaaggaatgt gatggttgtt gatgatctgt ctcacgtaga gagctttgtt actgttggtg
     2641 agcgaggcga agttattaat ctcgatgcgg atgggaacga actacacaca ccagacagtt
     2701 atcgtgaaaa gttaaaaata gaacgcggtg aactccaggc ttccttggag aaagccaagg
     2761 aggaaatcaa ctcaatagat aacgatataa cttcgcaaaa gattaagaac cagttgagtg
     2821 ctgcactatc tcaaccaatg gatgcagaag gtgagtggaa cactaagaga atcctacaag
     2881 gggttttaga tttatctaaa caagtgggct gtggtgtact cacggctgcg atcacgcatt
     2941 atcttggagt tagttaataa actacctatg cccccatggt gggtatacaa aaaccatggg
     3001 gagcggagcg accttcgtct agctcttttc ttgtgtgtct catcttgcaa cggattcgat
     3061 ttgtgtgggg ttagtgtcag acttcttggg gtcagtttgg atatagagaa ttattgtacg
     3121 gtaagcctgt gttttgaaca ggcttacctc tcgcaatgat gtactcccgc gctatacgcc
     3181 gtttcccgtg aattttcctt aatcggataa cctaaatgcg atacagaaag agcggtgact
     3241 taaaagcgat cgtagctgag gtaaaatcaa tcccttacgc atcaatattg gttaaataat
     3301 gcgcttaacg tacaaaagtt tccgatatcc aaactgaccc ctatttggcg gcttttctta
     3361 ttttcttgat tttcccgcat ctgaacgttt ctctcggact cttccattat tattacgatc
     3421 tcgtggctga gttccgcctc ctggtttagg gacgacgaca tgcgggttaa gtggtctacg
     3481 gtctgccatt ttcatacctc actctgcata aacaaatagt tgccagcgga tgtccccctg
     3541 tccgggacta tgttgatgat gattttgtgg tggaagtgtt ttccctcctg cgatagttac
     3601 ttctttaccg caacctgtgc atttgtaaat tccttcatac gtgcacgtat cgccaggctt
     3661 gtgttgtgta tcaaaaactg ggttaaattt cggatctgct tcaaaaatct tggttttatt
     3721 tcctgagcga acaagcgcca tatgacctcc aactattctt tttttgcaca gtagcactgt
     3781 gcttttgtgc attttatatc attatggttt tttgcacaag tgcacagtgc tattgtgcat
     3841 ttctggcgtt tgttgtacta tggtggtggt aaaaggtgat ctagaacttg ccaagttgcg
     3901 gcaacatttg aggtaaaaga aaaatgcagc aaaatcatga tagtgatgat gattgccgca
     3961 ttcagttccg agacgattgg ctataccagt tttataaata cggtaaagcc aatcgaaaaa
     4021 ttccagctac gattagtgtt tcattaggtc gcaagctgga tatgctgaat gctgctgata
     4081 aaattggaga tttacggtct cccccgggga accgatttga aaatctcaac cctccattga
     4141 cagggtattc atctataagg gtgaatgata agtaccgctt gatatttaag tggggtgaga
     4201 acggagtcga ggatttgtat ctcgatccac atgattaccg ataggtaaca gaccggaact
     4261 gttatcttac aaaacaagta ggtgaggcat ttcctcacct acgttaaaac gtcatcacaa
     4321 ataatgatta ataaagtaac gcgacgtaac tatatttaag ataattacta atcgatacag
     4381 ctggtaacag aagaaatact tatcttcctc atctaacaga gagaagcgac caattcagga
     4441 gtaaagcatg tcccaagcaa ccagaaaacc atcgactgta ggagatatta ttctttatga
     4501 atatctcgag
//
