LOCUS       NZ_CP079731             5640 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP3295 plasmid pKP3295-6, complete
            sequence.
ACCESSION   NZ_CP079731
VERSION     NZ_CP079731.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20170224
            Assembly: GCF_019334545.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Wu,Y.
  TITLE     The genome of KP3295
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Wu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUL-2021) Clinical Laboratory, Sir Run Run Shaw
            Hospital, Zhejiang University School of Medicine, Caihe, Hangzhou,
            Zhejiang 310000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:24:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,654
            CDSs (total)                      :: 5,534
            Genes (coding)                    :: 5,354
            CDSs (with protein)               :: 5,354
            Genes (RNA)                       :: 120
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 180
            CDSs (without protein)            :: 180
            Pseudo Genes (ambiguous residues) :: 0 of 180
            Pseudo Genes (frameshifted)       :: 71 of 180
            Pseudo Genes (incomplete)         :: 112 of 180
            Pseudo Genes (internal stop)      :: 31 of 180
            Pseudo Genes (multiple problems)  :: 31 of 180
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079731.1.
            Bacteria and source DNA available from The Fourth Affiliated
            Hospital of Zhejiang University School of Medicine.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP3295"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP3295-6"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="29.30 N 120.07 E"
                     /collection_date="2018-08-21"
                     /collected_by="The Fourth Affiliated Hospital of Zhejiang
                     University School of Medicine"
     gene            31..924
                     /locus_tag="KXI30_RS28065"
                     /old_locus_tag="KXI30_28055"
     CDS             31..924
                     /locus_tag="KXI30_RS28065"
                     /old_locus_tag="KXI30_28055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_044816547.1"
                     /translation="MNNAVLELFNDRLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRTGAAIDWNDRNAPAPTLTITNPDNGHAHLLYALRTSIRTAPDGKMK
                     PLRYAAAVENALRKKLEADAGYSGLICKNPNHSHWRIAVWQPELYTLDWLADFLDLNAA
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYDQWLQACYERATAYNLHFSSPLD
                     DNEVTGIAKSIAKWTSKNFSEDSFREFVSRTHTSEIQSIRGSKSKGGGRPSIGEPWLEH
                     GISRATYFRKLKKNEI"
                     /name="replication initiation protein"
     gene            914..2689
                     /locus_tag="KXI30_RS28070"
                     /old_locus_tag="KXI30_28060"
     CDS             914..2689
                     /locus_tag="KXI30_RS28070"
                     /old_locus_tag="KXI30_28060"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="WP_124046799.1"
                     /translation="MRFDASPFNMNSILSVEKRYEIPRNQREFSWERIQLEELWADLLR
                     NIVMDDNGTIKLHEYFIGTIVLSGVDSDDVLEVVDGQQRLSVITMMLSIISRYFRKNKN
                     DTTADNIYKKYIVTINNTLDRNSLNSDGESVIQKLTRSVGGEYFKKIVQGKEESDAKAT
                     NDEEKKIRYAVTFFKRALRKKSLCSAIFKTNDDSFTKADYDLCLNAIYKMITNYLYIVR
                     IAVDRGDDAYDIFEVLNARGINLSSIDLIKNKVFQSCTTTFPEDEARRKWVHIYEQLGS
                     VGETMTDYIRCWWLSKYGYIGEDQLYRSFKREILNENSLLTADSFLEEIYNDVSLYMSM
                     VAPSTKYWNLSHQRPILDALANINTFSVTVPRPFILSLLRTRRDKSRLMKQSELIETLE
                     FLEKFHFKFNAVCRMRPSGIDSKYSVFAVRADRAVSKASLKNTIDETVDFLKRKAPSEK
                     VFKDCLMKNIEYKDDKLSQRKLIIYIFETLERLAVGTKELKYHMVSIEHIGSQSNFSPT
                     YVGKLGNLLPLCFDINRDCENMPLAGKLPEYARSRLELVKQFNTANAAKNTWEVSDAQT
                     RQEQITNALNAAFTL"
                     /name="DUF262 domain-containing protein"
     gene            2921..3247
                     /locus_tag="KXI30_RS28075"
                     /old_locus_tag="KXI30_28065"
     CDS             2921..3247
                     /locus_tag="KXI30_RS28075"
                     /old_locus_tag="KXI30_28065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_038885858.1"
                     /translation="MEKRTKEIKIRLTETEHQRLLDRCDRTHLAEWLRQLGLGEHTSRK
                     RRVPEVAPELLRQVSGIGNNLNQIARRLNQADSLTPSERASLLVVLTSLDRQLGDLLEQ
                     NRDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3456..>3878
                     /locus_tag="KXI30_RS28080"
                     /old_locus_tag="KXI30_28070"
                     /pseudo=""
     CDS             3456..>3878
                     /locus_tag="KXI30_RS28080"
                     /old_locus_tag="KXI30_28070"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3925..4410
                     /locus_tag="KXI30_RS28085"
                     /old_locus_tag="KXI30_28075"
     CDS             3925..4410
                     /locus_tag="KXI30_RS28085"
                     /old_locus_tag="KXI30_28075"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_139914218.1"
                     /translation="MSEILNLAKAFEEKSKQQAQDTEQSVKREFARLETSISAELQSSS
                     QSISNAIREQNGRLTGMLRASVSTAMAWIFLCFALLIGIMGGIIWFQGTIITSRLSEMD
                     SYSQQLESLKAKSGAGVAIYSDDKHRNTYMVVLPKQAHGVETYTSTTDNTVVKYTAK"
                     /name="MbeB family mobilization protein"
     gene            4480..4704
                     /locus_tag="KXI30_RS28090"
                     /old_locus_tag="KXI30_28080"
     CDS             4480..4704
                     /locus_tag="KXI30_RS28090"
                     /old_locus_tag="KXI30_28080"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_223825645.1"
                     /translation="MEKQLLSALESLQTSYEQQQQAWQDSYSSLQHMFEATSQALTHSD
                     RVCQHLSSQVDSLRVQVESLSRNVSRLMR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4860..5192
                     /locus_tag="KXI30_RS28095"
                     /old_locus_tag="KXI30_28085"
     CDS             4860..5192
                     /locus_tag="KXI30_RS28095"
                     /old_locus_tag="KXI30_28085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_044816545.1"
                     /translation="MSHAIEFIETPMFTRQIKQIATDEELRELQKTLIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSLTDAEKAVLKSLTQ
                     QLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            5194..5478
                     /gene="nadS"
                     /locus_tag="KXI30_RS28100"
                     /old_locus_tag="KXI30_28090"
     CDS             5194..5478
                     /gene="nadS"
                     /locus_tag="KXI30_RS28100"
                     /old_locus_tag="KXI30_28090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_001697687.1"
                     /translation="MSFFDELKASLEEAVEIKNGVREPARVTRYELADVKAIRAQLNVS
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFQELAAH"
                     /name="nadS"
     Typing          3238..4796
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.743"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gacaacagcc cgccataagt gcagggcgct ttgaataacg cagtactcga acttttcaac
       61 gaccgcttac cgcacaagcc gtacttctcg gatgatttac actttggtgt acgcattgcc
      121 ggtaaggaac gcgccattct ggcgaaatac atccagttca accagccgca cgccatgttc
      181 tggctgggat tcgacgtcga tcgcaccggt gcggccatcg actggaacga ccggaacgcg
      241 cctgcgccca cgctgaccat caccaacccc gacaacggac acgcgcatct gctgtacgcc
      301 ctcagaacct cgatacgtac cgcgccggac ggaaaaatga agccgctgcg ctacgctgcc
      361 gctgtggaga acgcgctacg taaaaagtta gaggctgacg cggggtattc ggggctaatc
      421 tgcaaaaatc cgaaccatag ccactggaga attgccgtat ggcagccgga actctacacg
      481 ctggactggc tggctgactt tctcgacctg aacgcggcga acgacaaaga aatcgttgcc
      541 gactatggtt taggccgtaa ctgcacgctg ttcgacaaaa cccgcaagtg ggcataccgc
      601 gctatccgtc agggctggcc tgagtatgac cagtggctgc aagcctgtta tgaacgtgct
      661 acagcctata accttcattt ttcttcgcct cttgacgata acgaagtaac tggaattgcc
      721 aaaagcatag ctaaatggac aagtaaaaat ttttccgaag attcattcag ggagtttgtt
      781 tctcggacac atacctctga gatccagtca atacggggta gtaaaagtaa gggaggcgga
      841 agaccttcta taggggagcc gtggttagaa catggcatca gcagagctac atacttcagg
      901 aaactaaaaa aaaatgagat ttgatgcgtc accatttaat atgaactcca ttctttctgt
      961 agaaaaaaga tatgagatcc caagaaatca gcgagagttt agctgggagc gaattcagct
     1021 tgaggagctt tgggctgatc ttctaagaaa tatagtgatg gatgataacg gcactataaa
     1081 attacacgag tactttattg gaaccattgt gctatcaggt gttgatagtg atgatgtttt
     1141 agaagttgtg gatggacagc aacgattgtc tgtaataacc atgatgcttt ctataatatc
     1201 aaggtatttt agaaagaaca aaaatgacac aactgcggac aatatatata aaaaatatat
     1261 cgtaacaata aataacactc tggatagaaa tagtttaaat tctgatggtg agtctgttat
     1321 tcaaaagcta acaaggagcg ttggaggtga gtattttaaa aaaatagttc aaggcaagga
     1381 agagtctgac gccaaagcaa cgaatgatga agaaaagaaa ataagatatg ctgttacgtt
     1441 ctttaaaagg gctcttcgca aaaaatcatt atgtagcgcc atatttaaaa caaatgatga
     1501 tagttttacg aaggctgatt atgacttatg tcttaatgct atctataaaa tgataaccaa
     1561 ctatctgtac atagtcagga tagcggttga tcgaggcgac gatgcatatg acatatttga
     1621 agttctaaat gccagaggta ttaacctcag ttcaatagac cttataaaga ataaggtttt
     1681 ccagtcttgc actacaactt tcccagaaga tgaagccaga aggaaatggg ttcacatata
     1741 cgaacagcta ggttcagttg gcgagaccat gaccgattac atccgttgtt ggtggctatc
     1801 taaatatgga tatattggtg aagatcagct ctatcgatca ttcaaaagag aaattttaaa
     1861 tgaaaatagc ttgttaacag ctgattcatt tttagaagaa atatataatg atgtatctct
     1921 ttatatgagt atggtagctc catctacaaa atactggaac ctgtctcacc agagacctat
     1981 attagacgcc ttagcaaata tcaacacatt tagcgttact gttccaagac ctttcatttt
     2041 atctcttctc agaacaagaa gagataaatc aaggcttatg aaacagtctg agcttattga
     2101 gacgctagag ttccttgaga agttccattt taaatttaat gctgtatgtc gaatgagacc
     2161 ttcaggtatt gactcaaaat attcagtttt tgcagtcaga gctgatagag ctgtgagcaa
     2221 ggcttcattg aaaaacacaa tcgatgagac agttgatttc ctgaaaagaa aggcaccaag
     2281 cgaaaaggtg tttaaagact gcttaatgaa aaatatcgag tataaggacg ataaactatc
     2341 tcaaagaaaa ctcattatct acatatttga aacactagag cgactagcag taggaactaa
     2401 agagcttaaa tatcatatgg tttcgattga acatataggc tctcagtcga atttcagtcc
     2461 aacttatgtt ggtaagctgg gtaaccttct gccgctttgt ttcgatataa atagagactg
     2521 tgaaaatatg ccattagctg ggaaattgcc agaatatgct cgatcacgat tagagttagt
     2581 gaagcaattt aacactgcta acgcagctaa aaatacttgg gaagtaagtg atgcccaaac
     2641 gaggcaagaa cagataacaa acgccctcaa cgctgccttc acgctctgat gtgagactaa
     2701 acaacctata tcagataaca gccctcttta gcagagggct gtttttttgc ggatagcata
     2761 gaatctgaca tactacttca tactataagc cagtacacac agcgtcccct cttacgaggt
     2821 gtcgccgtga ctggttcagg gggctcgccg ccccccgaaa cccccagcat ccactgcgaa
     2881 aaattctcac ggtggtgcga attttttctc agcggagcct atggaaaagc gaacgaaaga
     2941 gatcaaaatc cggctcaccg aaaccgagca tcagcgactg cttgatcgct gcgaccgcac
     3001 tcaccttgcg gagtggctgc gtcagcttgg tctgggtgaa cacacctccc gcaagcgccg
     3061 tgtgcctgag gtggcgcccg aactgctgcg gcaggtcagc gggattggca ataacttgaa
     3121 ccagatcgcc cgtcgcctga accaggctga ttccctgacg ccttctgaac gtgcctcgct
     3181 gctcgtcgtg ctgaccagcc ttgaccggca gcttggcgac ctgctggagc agaaccgtga
     3241 tcgttaaaat tcactcacgg ggtgcgggtt cgggcagcgg gccggtggat tacctgctgg
     3301 gtaaagaccg gcagcgtgaa caggccagcg ttctgcgcgg caaccctgaa catgtgcgcg
     3361 agctgatcga cggctgcgac tttgcccgcg cttatacttc cggcgtgctg tcatttcagg
     3421 agccggacat tgcagacgct gagaaatcgc gcctgatgga cgaatgggag cacacgctgc
     3481 tgaccggcct tgatcgcgac cagtacgcct gcctgtgggt ggagcaccgt gacaagggac
     3541 ggcttgagct gaattttgtc atcccgaaca tcgaattgca gagtggaaaa cgcctgcaac
     3601 cctactttga ccgcgccgac cgtccccgcg tcaacgcctg gcagaccctg accaacgacc
     3661 ggctggggct gcgcgacccg aatgacccga cctaccgccg tccgctgacg caggccagcg
     3721 atttgccccg cgacaaacag caggcggcag agaaaatcac cgccggcctg atgaacctga
     3781 tgcagcaggg ggtgatccgc agccggcagg acgtggtgac gcagctggaa agctacggcc
     3841 tgaccgtagc gcgggaaacg aaaagcagca tcagcattgc cgatccggac ggcggccgaa
     3901 atatccggct gaaaggaatg atttatgagc gagattttaa atttggcgaa ggccttcgag
     3961 gagaaatcga agcagcaggc gcaggatacc gagcagagcg tgaagcgcga gttcgcgagg
     4021 ctggagacgt ctatcagcgc ggaactgcaa tcaagctcgc agagcatcag caacgctatc
     4081 cgcgagcaga acggcagact gacgggcatg ctcagagcct ctgtctcaac ggcgatggcg
     4141 tggatattcc tctgcttcgc cctgctgatc ggcatcatgg gcgggataat ctggtttcag
     4201 ggcacgatca tcacctcgcg cctgagcgag atggacagct acagccaaca gctggagagc
     4261 ctgaaagcga aaagcggcgc gggcgtggcg atctacagcg acgacaagca ccgaaacacc
     4321 tacatggtgg tgctgccgaa gcaggcgcac ggcgtggaaa cctatacatc gacgaccgac
     4381 aacaccgtag tgaaatacac ggcgaaataa ccccgtcaga gcgccctgaa acgacacagc
     4441 gcgtttttaa caccgaggat gaccaaaacc atgaccgaga tggaaaaaca gcttctgagc
     4501 gccttagaga gcttacagac aagctacgag caacagcagc aggcgtggca ggacagctac
     4561 agcagcttgc agcacatgtt cgaggctacc tcacaggcgc tgacgcacag cgacagggtg
     4621 tgtcagcact tgagcagtca ggtggacagc ttgcgcgtgc aggtcgagag cttaagcagg
     4681 aacgtcagcc gcttgatgcg ctgattgcac agcacgaccg cgcccgtgcg gagaagctgc
     4741 gcgagcagca gccggagccg cagaaaaagc cgacccgcta ttacggcccc tcgatgtgac
     4801 cgcttttctg aaattaaaat taagtgtact atgtacgcca ttgaacacag gagacagccg
     4861 tgagtcacgc gattgagttt atcgagaccc cgatgtttac ccgtcagata aaacagatcg
     4921 ccacggatga agaactcagg gagctgcaaa agacgctgat tgagtcaccg gataaaggcg
     4981 atctcatcca gaaaaccggt gggctgcgca aaatcagaat ggcgacgggc aaccagggaa
     5041 aaagcggcag tgcgagggtt atttactttc tggccaccgc cgaggtgatt tatctggtga
     5101 tggcctaccc gaaaagcacc aaagacagcc tgacggacgc ggagaaagcc gtgctgaagt
     5161 cgctgaccca acaactgaaa gacgaggtgt gacatgagtt tttttgacga gctgaaagca
     5221 tcgctggaag aagccgttga aatcaaaaac ggcgtcagag agcctgcccg cgtcacccgt
     5281 tacgagctgg cagacgtgaa agccatcagg gcacagctga acgtatctca gagcgagatg
     5341 gcgaaagtgc tggggaccag cctcgacact atcaagagct gggaaaccgg acgccgtaat
     5401 ccgaccggac tggcggcgaa ggtgctggca accatccagg cgaacccgaa attcttccag
     5461 gagctggcgg cgcactaagc cgttgtgaca gaaggattcg acaggcgagg ccgttcgcgt
     5521 gtaatttaac ggcaacagaa aaaacaaagc cccggaaatc atgctctact ttggcgagcg
     5581 gacatgataa ccagggccag gtcgaaacct tgaaaggata ttagcacatg aaccgtgcca
//
