LOCUS       NZ_CP079729             9730 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP3295 plasmid pKP3295-4, complete
            sequence.
ACCESSION   NZ_CP079729
VERSION     NZ_CP079729.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20170224
            Assembly: GCF_019334545.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Wu,Y.
  TITLE     The genome of KP3295
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Wu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUL-2021) Clinical Laboratory, Sir Run Run Shaw
            Hospital, Zhejiang University School of Medicine, Caihe, Hangzhou,
            Zhejiang 310000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:24:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,654
            CDSs (total)                      :: 5,534
            Genes (coding)                    :: 5,354
            CDSs (with protein)               :: 5,354
            Genes (RNA)                       :: 120
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 180
            CDSs (without protein)            :: 180
            Pseudo Genes (ambiguous residues) :: 0 of 180
            Pseudo Genes (frameshifted)       :: 71 of 180
            Pseudo Genes (incomplete)         :: 112 of 180
            Pseudo Genes (internal stop)      :: 31 of 180
            Pseudo Genes (multiple problems)  :: 31 of 180
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079729.1.
            Bacteria and source DNA available from The Fourth Affiliated
            Hospital of Zhejiang University School of Medicine.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP3295"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP3295-4"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="29.30 N 120.07 E"
                     /collection_date="2018-08-21"
                     /collected_by="The Fourth Affiliated Hospital of Zhejiang
                     University School of Medicine"
     gene            join(9632..9730,1..987)
                     /locus_tag="KXI30_RS27975"
                     /old_locus_tag="KXI30_27965"
     CDS             join(9632..9730,1..987)
                     /locus_tag="KXI30_RS27975"
                     /old_locus_tag="KXI30_27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WP_063938519.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     gene            984..3173
                     /locus_tag="KXI30_RS27980"
                     /old_locus_tag="KXI30_27970"
     CDS             984..3173
                     /locus_tag="KXI30_RS27980"
                     /old_locus_tag="KXI30_27970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WP_063938521.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     gene            4759..6720
                     /locus_tag="KXI30_RS27985"
                     /old_locus_tag="KXI30_27975"
     CDS             4759..6720
                     /locus_tag="KXI30_RS27985"
                     /old_locus_tag="KXI30_27975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_063938523.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            6720..7451
                     /gene="mobC"
                     /locus_tag="KXI30_RS27990"
                     /old_locus_tag="KXI30_27980"
     CDS             6720..7451
                     /gene="mobC"
                     /locus_tag="KXI30_RS27990"
                     /old_locus_tag="KXI30_27980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071653262.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            7458..7988
                     /locus_tag="KXI30_RS27995"
                     /old_locus_tag="KXI30_27985"
     CDS             7458..7988
                     /locus_tag="KXI30_RS27995"
                     /old_locus_tag="KXI30_27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938511.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8016..8195)
                     /locus_tag="KXI30_RS28000"
                     /old_locus_tag="KXI30_27990"
     CDS             complement(8016..8195)
                     /locus_tag="KXI30_RS28000"
                     /old_locus_tag="KXI30_27990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            8264..8641
                     /locus_tag="KXI30_RS28005"
                     /old_locus_tag="KXI30_27995"
     CDS             8264..8641
                     /locus_tag="KXI30_RS28005"
                     /old_locus_tag="KXI30_27995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938513.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="hypothetical protein"
     gene            complement(8703..9002)
                     /locus_tag="KXI30_RS28010"
                     /old_locus_tag="KXI30_28000"
     CDS             complement(8703..9002)
                     /locus_tag="KXI30_RS28010"
                     /old_locus_tag="KXI30_28000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_063938515.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8992..9255)
                     /locus_tag="KXI30_RS28015"
                     /old_locus_tag="KXI30_28005"
     CDS             complement(8992..9255)
                     /locus_tag="KXI30_RS28015"
                     /old_locus_tag="KXI30_28005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938517.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     Typing          complement(3379..3959)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.625"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6721..7448
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.885"
                     /coverage="100.0"
                     /evalue="3.84e-144"
ORIGIN
        1 gaatcaacat tcctgactcg tgatatcggc aatggtctgt atgaaatgtc ttttgatggc
       61 aaaaataata gtctctgggc agcatcatcg tcttcattcg acagagataa gggccacgga
      121 attgtttatc agttagaacc atccactctg aaaacaaaat ctgccgttaa aatcgcacgt
      181 cttgggtttg ccacagcact ggatgaggaa catgagcgac tttatatcgg caatactctg
      241 gacggctcag tgacggtcat tgatactcta accggaaaag aactttcagt aattccgtta
      301 actgagggac aggatgaggc tcatttcaga ccgaccagag aaatggtcat tgacaaaaaa
      361 aatcagcgtc tttatgtctc cagcgccgca gataaagggg ctctgtgggt tattgacatg
      421 gctgtcggga agaaaattaa aaccattgat ggactggggc acgctgttac tggaattgcg
      481 ttaaataaaa atggggataa tatttatctg gttaacgggg atggtgagat tattaacctg
      541 gatgccagta gctatgaaat aaaaaaacgg tttgttgtag agcctgagaa aaaacacttt
      601 ttccttaatg taagtctgga tgaaaaaaac ggacgtgcat ttattaccga cccggatatt
      661 cccggggtgc tggttgttga tgtaaactcg ggaaaaatac tacacagaat tgatgttata
      721 aattcactgg cgattttatt taatccttta cggaacgaaa tctatatcac gcaccgtaat
      781 gcaaaacagg tcagcattgt ggacagtaat tcttattacg taaaaacaac gataaaaacc
      841 cgcctgatgc caaacagtct gtctttatca catgacggtg aaaatctgta cgttagtgta
      901 aaacaagaaa aaaaagagat gagtactatg aaggattaca tactcaaaat cgaattatct
      961 aaaattgcta tggagccaga agaatgaata aatatttagg gctgagcata gtatatatga
     1021 tgtatacatc atctgttatg gctgatgatt ctggcgcagt tagtaaagat aaaaatattt
     1081 caccagaaga aacattaatt gttcatggga ccgctgtttc tgaaagcaca acagaaggaa
     1141 cagattcata cacaacccag tcagccaata cagcatcaaa gatgaatctg tcattccggg
     1201 atacacctca gtcaactaca gtcattacgc gacgaagaat ggatgatgta gcagcaaaaa
     1261 atattattga tgtggttaaa accgctcctg gattatatgt cagtacagca gaaggttccg
     1321 gaagaccgaa ttttatgtcc agaggattta atgctaatgt catgtatgaa ggatttagcc
     1381 tgccgtggaa ttcatacatt ccaagttcac aaactagttc agctattttt gaccgggtgg
     1441 agatagtccg cggggcaaca ggtcttgttc agggtgccgg agacccttca gccgcgataa
     1501 atatgataca caagcgtccg acccggcaat atcaggccag cactaacatc ggtgccggac
     1561 ggtggaatga ctaccggggt ggggttgatg tatcaggacc actgaatgac agcgcgacct
     1621 tacgcggaag ggcagttgtg gatggcgaaa acagtgagac cttcagggat agtgagaaac
     1681 gcaggaataa cgtcatttac ggaattatgg aggcagatct gactgataat accctactta
     1741 cgattggggg atatcagcag tatgaatata acaatcattg gtggtatgac ttacctattt
     1801 ctggtaccgg acaccacctt aatcttcctc gctcgacgtt tacaggcaat gactgggagt
     1861 ttgaaagaag taaaagtaaa actgcatttg caactcttga gcagaagttc agtaatagct
     1921 ggaagctgaa attttcaacg ctgcaaaact ggagaaatgc tgacctgtta gggacggcaa
     1981 cataccggca taaagatacc cgacaaagta tgtatcagca agtatgggga tcactgaggg
     2041 aatataattc caaaagctat gacctgtcgc ttaatgggga ctatgcttta tttggtcgtg
     2101 ttcatcaatt cgtggctgga acaactcagc aaaaatacac cgataaagat acaggttata
     2161 catattcccc aatatatatt gagaatgtta atatatttga tcatggttca cattcttcga
     2221 aaaaacccat gaagatgtca ggtgatgcat cctacgacgt cactgaacaa aaaagcgcct
     2281 acgcagcaac tcattttcta cttagtgata actggaaaat attgctgggt ggccgtcttg
     2341 actggtatga atatacaagt aaaggtacat ccattaatac aggttataaa gttaccagaa
     2401 atatcaccaa atatgcaggt tcaacataca atcttgacga tcatcatacc atttatggga
     2461 gctataccga tatattcaat ccacagtcaa gcaccgacct caatggtaaa gtgatcaagc
     2521 ctattgttgg taagaattat gagacaggta ttaagggtga gtattttaac ggcttgctta
     2581 atgcctctct ggcggtattt caggtagacc aggaaaatcg agccaggctg cttcccgatc
     2641 agacagggtg tgttactaat ggcaatcctc aatgctatga ggctagcggt aaagtacgca
     2701 caaaagggat agatttcgaa cttcagggca tggttacgcc actctgggaa attggggtgg
     2761 gatatacata tgcagatacg cgatatataa aagatggaga tcgtagtaat gaaggcaagc
     2821 gattcaatac tgccgtgtcg ccacaaaatt tatttaagat ttcgagcatg tacacctttg
     2881 acggaaaatt aacaaactgg cgagcaggag taaacacgta ctggcaaagt aaaatatatg
     2941 ctaacataac agacacaagg gatggcaatc cggttattaa taaacaaaag gcttattcaa
     3001 caactgacat tattattggc tatactccaa tgccacaact ggatttacag ctcaatataa
     3061 ctaatgtctt tgataaggtt tattatacat ccataggtga atcgattgga tatggcacgg
     3121 aaacatatgg agaaccaaga aatgttaaaa ttagtgcaac ctatagattc taaccatgcg
     3181 gattgggtct tctcctcgga tttgccccta taaatccgtc ttttgtggtt ccggcgtaca
     3241 accccctgtt tgccacttca gttgatggtt aatcaaatca tgtagaggat cttcttgaga
     3301 tcctccatgg ttcgtcgtaa tcttttgccc tgtaaacgaa aaaaccacct ggggaggtgg
     3361 tttgatcgaa ggttaagtca gttggggaac tgcttaaccg tggtaactgg ctttcgcaga
     3421 gcacagcaac caaatctgtc cttccagtgt agccggactt tggcgcacac ttcaagagca
     3481 accgcgtgtt tagctaaaca aatcctctgc gaactcccag ttaccaatgg ctgctgccag
     3541 tggcgttttt gcgtgtcttt ccgggttgga ctcaagtgaa cagttaccgg ataaggcgca
     3601 gcagtcgggc tgaacgggga gttcttgctt acagcccagc ttggagcgaa cgacctacac
     3661 cgagccgaga taccagtgtg tgagctatga gaaagcgcca cacttcccgt aagggagaaa
     3721 ggcggaacag gtatccggta aacggcaggg tcggaacagg agagcgcaag agggagcgac
     3781 ccgccggaaa cggtggggat ctttaagtcc tgtcgggttt cgcccgtact gtcagattca
     3841 tggttgagcc tcacggctcc cacagatgca ccgaaaaagc gtctgtttat gtgaattctg
     3901 gcaggagggc gaagcctatg gaaaaacgcc accggcgcgg ccctgccgtt ttgcctcaca
     3961 tgttagtccc ctgcttatcc acggaatctg tgggtaactt tgcgagagtt agcagcgccc
     4021 gccgcagtct cacggccgga gcgtagcgac tgagtgaggg aggaggcgca atatcccccc
     4081 tcccgcagac ggccaccgga cgcgcggatg tgacatattg caccgtgtta ccgcctttcc
     4141 cttccctgcc gataaatcat tcagataggt gaagtctgtc ggttttctgc tgaatatttt
     4201 acgttaacct atctctgttg cttttttctc cttgcggaga aatgcaggtc atccccgctg
     4261 gggcgtggct gacttcagga ggaaatcccc tgcttacgct ggctggacgc caccgacgca
     4321 cggtctttcc ccctctttct ggctcagctc ggctggacgc cggccacgcc agaaattcac
     4381 ccggcccccg tcaggctcac gcctgaccac ccacaaaaac ccacggtgct gaacgcacct
     4441 gacgaagcag caaagctgct cctgattttg cgggtggtca gccgctcgct atggcgaact
     4501 ctgacggggg ccggaacacc ggaaaagacc gcactggggt gccgcctttt cctctcacac
     4561 gctggggcgt gctcgttgtt cctccggggc tgcgcctttt ccgcttgggg cggaaagagg
     4621 ctggcctccg tggtgaaagc tgaatgcaat caccacggac gtgaccgccg ccgccatgcc
     4681 tcggggcatg tccgcggctt gtgcagcagg ggctgcacac cacgccggta aacccgtcgt
     4741 ctgaaggaga gagattgcat gtttaatacg gattcgcttg cctggcagtg gcatatcccg
     4801 gagctggccc ggcagattta cccgtgggtc agctggctgt acagggagta ttacggtctg
     4861 gccggggctt tcctggttat cggcctgttt ttcggtctgg ctgaactctg ggccggacgg
     4921 atgcgttttg tggggctgag tcataaggag cgacgggagt atgccgatcg ctataagcgc
     4981 gataataacc ggactgcgcc attttcggtg ctggcttcgc tggattcgcc cgggcggatg
     5041 gtcagggcac ggcggcatgt ctggaactac gcgtactggc tgaccggcgc tgccggtctg
     5101 gcccttctgg gggggctggt tgtcgccgtg tttatcgaga gctggggaga aaccgtcctg
     5161 cgtccggacc tctacgcgaa cgcagattat tacctgctgc agccggctaa cctgttcggg
     5221 attgtctggt gtgccggagg gcttgtcgcc ggcatccttc tggcgcggct gctggcattt
     5281 ctgctgcttg acggtcattt tgcggccgct gacgaggcgg tgaatgcgaa actgaaccag
     5341 gagagccggc gcagcagcca gcggaccgga gaaatgaccg acgtccgcca cctgcatttc
     5401 ggggagccgg taccggttaa cgcgctggcc gatttttcca cagagcaggc ccggaaacag
     5461 caggcggtgt ttctgggtaa ggatgagcag ggccagccgg tgctggtccc gcgtgatacc
     5521 tggcgcaaga ccaatattca gatcctcggc ctgcccggga gcggcaagag cgtgatgggc
     5581 accaacgcgc tcattcgctg cgtacgcgat ttcggggacg cggtggtgta cttcgacccc
     5641 aaaggggacg cgtgggcgcc gcacgtcttc cgggcgcact gcccggactt caccctgctg
     5701 gatctgcggc cggggaaacc ggcgcagctg aatctgtttc gcgatctcga ccagtacgcc
     5761 ctgaagaatc tgctggttgc cgggtttaac ctgagtgaga ccggcgacgt ggcggaccac
     5821 taccgcatca gcgagcagaa agcggcgaag ctgattgcgg agcagttccc gcagggggca
     5881 aacattcagc aggtgctggc ggcggcatac gcgctgccgg aggccctgaa aaaggacgtg
     5941 aaggggctga tcaccaagct ggagaacgtg gccgacctga gcgtcctgca gacggacagc
     6001 ggcatcgacg tggcggggat aatcaacggc ggcggctgcc tgtacgtcat cggctcgatg
     6061 gacgatgagg cggtgatccg ggtgcagaag atgctgtttg cccgctgcgc gcaaatcatc
     6121 attgcgcggg atgagttccg gaggtggccg cacgcgagca tcatgctcga tgagattaaa
     6181 tacctgctgt cgaagtacgt gctgaacgcg ctggggacgc tgcgcagtcg ggactgcaac
     6241 ctgctgctcg cgcaccagtc gctgggcgac ttcgggcagt gcggccagga tctgcccgct
     6301 gagtttgtga aaaccacggt gctggataac acgccgatcc gctggttcta ccgggcggcc
     6361 agccaggagt ccgcgcagtg ggcggcgggc cagaccgggg aaatccgggt ggacgtggag
     6421 cgccgccggg ccagccggga ggcggggaac gtggagcata tcagcgggga cagcttcatt
     6481 cagaaggagg cgcggccgct gtttgacgtg aacaccctgc agcacctgcc tgacgggttt
     6541 gcggtgatga ccgggctggg cgtggcccgg atggcgttca gcagcccgct gcgggttgaa
     6601 cgccgggaaa tcccgctgca gcgcttcccg gtgctggcga aaacggaccc gctggcggag
     6661 taccagccgg aggccgggcg gcagcggccg ggtgatgatg attttgcggg gctgtactga
     6721 tggcactgga gcgatacaac gtcagccacg cgaagcggca ggcgcggaac gcggagaaaa
     6781 cccggctgac cctgcgctgg ctgcgggaag agctgtgttc cacggcggag ctggttgcgc
     6841 gacggctggg gattgcggcg gtgcagccgg tttaccgctt cctggacagc ctggtggcga
     6901 aagggctgct ggtccgggcg aaatacccgg ttgacggccg tcaggtcagc gtgtggggac
     6961 tgacgccgca cggggtggcg ttctcgttcg acgaggacga accgctgacg gacgttattc
     7021 cgtttcagcc gtcaagggtg tcggcggcgc agctgccgca ccggctggcc gtgcagtccc
     7081 tgcgtctcgc catggaggcc cggggggcct ccggctggcg gtacctgcac cgtatggccc
     7141 tgaaggggat gaaggtgccg gacgcgctgg cggagctgga cggcagaacg gtggcgtttg
     7201 aggtggagcg caccgtgaag agccgcaggc gctatcagga ggtggtgtcc gggtatctgt
     7261 tcaatcgcaa agcgaacggc atcgatgaaa tctggtacat ctgcccggac cgggcgacgc
     7321 aggtcagggt gcagcgggcg atcctgtcgg tggatgaaat cgtgaatcca cagaccggcg
     7381 aggcccgcaa aacggcggag ctggaccggg agcggctgtt tgcgtgcttt aaatttatga
     7441 caacggagtg agggattatg gcgctgtaca tggtatttat tgaagacgcg gaggaccggg
     7501 acggcgagcg ctgcatcatg ccgaccagcg agcatcttga agatatggtg gtgctcgcct
     7561 ggcagaccgg ggcgtatgtt ttctgttttg tcgaccgggc ccagcatctg gcgatgatga
     7621 aatccatcgg gatgtatgac gagcggctca acagcgacga aaaagccacg tactttttca
     7681 ccggtaaaaa tccgcaatgg gaacgggaca aacccgcagg aatgaagctg tgtgattacc
     7741 tctatcgcaa ggtggagcgg ttcgccatcg agaacggtgt gcaggtggtt gatgccggga
     7801 agtgctcacc ggagcagatt agcgcgatga ttgaggtggt cagcgagcgg cacggggaaa
     7861 gcgcggtgaa cgagaagctg aaagcggagc tgatgcggga atataacagc cgctatttta
     7921 acgtgccggt gcctgaactg ccggaggaca aacccgcccg ccggtggtgg ccgttcggca
     7981 aaaaataaac ggcagcaccg gccatgccgg tgctgttatt ccttcccgag cttatcccgc
     8041 atcacctgcc agagtgtttc cgcatcctcg tgcagccgct cgcacatgtc ggccggggta
     8101 tccatatcca gctcgttcag tttctcgagt aacagcagcg actggtcctt aataaattta
     8161 gccatattga gggcagtttt ttcctgctta gtcatgtttt ctgtccttcc gggtgtggtg
     8221 tgcttcctgt cgggtatact gtctcaaaac cggaggtgag acaatggcgt gggtgacggt
     8281 gagacaggcg acggaactga cggggaaagc ccggagttcc ctgtataggg atatggcgaa
     8341 aggccgcgta tcataccgta ctgaagctga tggtggtcgg gtggtggata ccagcgaact
     8401 tatccgggtg tacggtgaat taagacagat tgagactcac gagcgggacg ggttgagact
     8461 gtcggatgag actgaaaaaa tggcctccga cgcgttgatc gcggagataa aagcactcag
     8521 ggaggaggtg gccggactgc ggcaggaaat gcaggccatg agactgctgg aacacaaacc
     8581 agagacagca gagacaccgg ccggaacatc gcgccgatgg tggcagtggg gaaaacgctg
     8641 acgcgctgtg cttgtccaac cggcgacggc cacgcaatga attgcgcccc catcaccggt
     8701 tatcagcgaa cggtatgggg attctggcgc tggccaacca cggccagaac ccggactttc
     8761 ctggtctggt catcaacatc atagaggaca tgctgtccca tcagactgat ccggcgaacg
     8821 tcctcgccat agcgtgagtc cacttcatat aggcgcggga agtcggccag tttgtccgct
     8881 gcagcgagaa ctcgatcggt aacgttctgc gcatcccaca gggatgaatt ttccgcaatg
     8941 tagtcaaaca atgctgtcac atcctcgatg gctccctgct tccattccac ctcatacggc
     9001 atcgcgtagt tcctgatagc ggccatgcag tctctggcgg agttgttccg tggtcatgcc
     9061 cgggcgcgtg tcagcgcggg cggcggtcac tttagcgcga acctgatctt catattctgc
     9121 ctgggccagt tcttcgttag ccaggcgctg caacagtgca gctttgtccg ggccaccttc
     9181 aggcagtatg cggggcagtc tctcggccag ttcccgcagg aaattggcgt tttcatagtg
     9241 tgtactgttg ctcattgtgg agcctcccgg gctgatacgt cgatatgaag gagtttagca
     9301 gggggcggtc tgaaagaata tgctcgtgtc agtcaggctg atgaagacga aagctggtaa
     9361 aaaagggctt acgacaccca ttttacgtca aactgcacga catgcagttg cgtgcatagc
     9421 catgcagcgg gttaaaattt atcaggcgcg tttccggcgg ttttccgggt ggtttgttgc
     9481 cggttttatc ccgtagccgc cggaaatgcc ctcagaccgt ctgagcggtg cgcgtaatgc
     9541 gacgttatgg taaatcatgt gattaatggt agtgataatc attatcactt ttgctatatt
     9601 cttgtttctt ttattgtaag gaaatgtaag tatggcaatc acgattaaat ccgccgttaa
     9661 tgtccgtagc agagtattaa tatactcttt actactgttt actggttcag cgtatgcggc
     9721 tgatatagct
//
