LOCUS       NZ_CP079697             4991 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC31_S468MPCH
            plasmid pS468MPCH_P5, complete sequence.
ACCESSION   NZ_CP079697
VERSION     NZ_CP079697.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20130733
            Assembly: GCF_019317265.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC31_S468MPCH
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC31_S468MPCH
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 91.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:20:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,681
            CDSs (total)                      :: 5,555
            Genes (coding)                    :: 5,411
            CDSs (with protein)               :: 5,411
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 62 of 144
            Pseudo Genes (incomplete)         :: 78 of 144
            Pseudo Genes (internal stop)      :: 23 of 144
            Pseudo Genes (multiple problems)  :: 17 of 144
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079697.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC31_S468MPCH"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pS468MPCH_P5"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2014"
                     /collected_by="Christian medical college"
     gene            complement(86..490)
                     /locus_tag="KW564_RS28260"
                     /old_locus_tag="KW564_28235"
     CDS             complement(86..490)
                     /locus_tag="KW564_RS28260"
                     /old_locus_tag="KW564_28235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="WP_008321396.1"
                     /translation="MTILVTIQAQLVVGEEQVIKSLAPEGMLAAVFEDDGRTGYFYALD
                     ESVEGNPIMDAVHIYNAEDVSDGHIPSDVKIGWSEDSQKCVLLINGYPHGAFDFVGKNG
                     YCRSGYPPPINKVWSLSGHEWSDSVDDFFR"
                     /name="DUF2251 domain-containing protein"
     gene            complement(1285..1686)
                     /locus_tag="KW564_RS28265"
                     /old_locus_tag="KW564_28240"
     CDS             complement(1285..1686)
                     /locus_tag="KW564_RS28265"
                     /old_locus_tag="KW564_28240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032489494.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQLKGEHHSGQVSEQRDTASVDQCEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1686..1877)
                     /locus_tag="KW564_RS28270"
                     /old_locus_tag="KW564_28245"
     CDS             complement(1686..1877)
                     /locus_tag="KW564_RS28270"
                     /old_locus_tag="KW564_28245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032489493.1"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2293..2459
                     /locus_tag="KW564_RS28750"
                     /pseudo=""
     CDS             2293..2459
                     /locus_tag="KW564_RS28750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            2789..3847
                     /locus_tag="KW564_RS28275"
                     /old_locus_tag="KW564_28250"
     CDS             2789..3847
                     /locus_tag="KW564_RS28275"
                     /old_locus_tag="KW564_28250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508929.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_042109829.1"
                     /translation="MAIYAVWNNKGGVGKSYLTFQLASEYARQNPTKKVLVVDLCPQAN
                     SSSMLLGGMINGENTLNQIHSAIQRKTISGYIEDRIRSPYIPTNTGSSYVINVSTHNSK
                     VPNNLYLVTGDEQLELQASRVVGASFPGPHDAWRTVHLWINDLISDICNSWNNEDSCVF
                     IDCNPSFSIYTELALTAAERLLIPFSADGSSKRAVKAVLSLVYGERRHQNEPQSDFYLN
                     SNRYSMSLPQIYMYVGNRLTQMNNSSASAFKTVVTEIGDEIWKVWQTKPSLFCIHPNGS
                     STPNNKRSFRNMFQYEVNDANTASVVSGALGIPIATLTAGNKTVSGKIIKVNQTQLDKQ
                     VPNIVALAGMIE"
                     /name="ParA family protein"
     gene            complement(3877..4134)
                     /locus_tag="KW564_RS28280"
                     /old_locus_tag="KW564_28255"
     CDS             complement(3877..4134)
                     /locus_tag="KW564_RS28280"
                     /old_locus_tag="KW564_28255"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042109830.1"
                     /translation="MGSNNNQNKDLQDKQADLLDQTYTLGWMDGYAEGYDDAIEDTQNA
                     LNKLENENELLTEVVESKDNVIKELASSAGRALQAVKKNK"
                     /name="hypothetical protein"
     gene            4552..4983
                     /locus_tag="KW564_RS28285"
                     /old_locus_tag="KW564_28260"
     CDS             4552..4983
                     /locus_tag="KW564_RS28285"
                     /old_locus_tag="KW564_28260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_042109831.1"
                     /translation="MKIPAHLKHKPIIQVENYDRIDGPHADDTDAMGLSVGIAQWNTPG
                     WTELSAKVWRNTGEKWSRQSEELPLHRVIDLATLICITMDYSKNGRLSSSDDFPVTRST
                     DNPELAHHTEFMKKELKQNKSNLDVSLKRLSTELKRLGY"
                     /name="DUF6530 family protein"
     Typing          1999..2401
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.857"
                     /coverage="99.0"
                     /evalue="7.72e-165"
ORIGIN
        1 ttagaaatgt ctacctactt cacagacgaa tttaccataa catccgtttt tcgaaccata
       61 atggcaggct atagcctgcc gtaattcacc ggaaaaaatc atccacagag tcgctccact
      121 catgcccgga aagagaccac accttgttta tagggggagg ataaccactc ctgcagtacc
      181 cgttttttcc cacgaaatcg aatgccccgt gcgggtatcc attgatcagg agtacgcact
      241 tctgactgtc ttctgaccag ccaattttaa catcagaagg aatgtgaccg tctgaaacgt
      301 cttcggcatt gtagatgtgt actgcgtcca taatcgggtt tccctcgacg gactcatcga
      361 gcgcatagaa atacccggtc cggccatcgt cttcgaacac ggcggccagc atgccctcag
      421 gagcgaggct ctttataacc tgttcctccc cgacaacgag ctgggcctgt atcgtcacta
      481 aaatggtcat caggtctgtt ctcctttcct tggttgcttc ggcagccagc tttcagacaa
      541 gcggataatt taccataaca tccgttatca gcatcgtcag aaacgcgctg agggcgtttc
      601 tgacgggaaa ccggtaaaac aggcaacaaa ccacccgaaa acccgccaga aacgcgcagg
      661 ctctgttttt atggcatgca tgagtatgca cgcaagtgca tagcgcgaat attcacgcca
      721 ttcagacagg gtaagtcctg tttccggcgt tcttcccatc cgaaaacggt gaacggggac
      781 gcaatttatt gcgtggccgt gaagcgttgc cggcgcagcc ggaaaaccgg ttttcagcac
      841 gttcaggaat cgggttcgtc gacggccggc aatcacgtta tggcaagtcg ccggacgact
      901 caccacacga ttcaccggcg actctgcact agagtcgtac ctagagtcgc cgcgactaaa
      961 aaacaaccaa acggggagtc gggtctagag tcgggtcgtt attaactcat tgtttttata
     1021 gccctgagtc gttaccgact ctatgacgac tcgccgggga ctctatgacg agtcgtcata
     1081 gagtcggatc tatataaata aaatttaaaa aacgaaatct caggaaagta aactactgat
     1141 attaaataca aatctgcaaa attttaattt tgcgtggggt gtgggtattt ttagtggtga
     1201 ggagcgttca gagatctgac tatttttggg aaggttcagg aactccccgg gatggccgga
     1261 gaggaaaact gaagcatgac agggtcagga ttttttccag aatttccacc atggcgccgg
     1321 ggattcagtt aattttgcct ggtgattttc aagaaggagc atggcctgtt tcaggctgtc
     1381 aatgtgcgaa tctttggaca aaagtaaagt gttcaggtgt tcaatctcct gtttcagatg
     1441 acgaatcacc tgttcacact ggtcaacaga ggctgtatcg cgctgttcag agacctgtcc
     1501 agaatggtgt tcacccttca gttgttcaaa gggaccaaag acacgtaaaa gctctgaagt
     1561 atctaccatt tttgcattgc gctcaccgct acaggtggac aggtcacctg ctttaattaa
     1621 cctgtgcagt gttgttctac tcttacccgt cagtcgggct gcctcactaa tagacactct
     1681 ggccatcagt taccatcctg taatgcatcc agtctcgagg acagtgtacg gaaaagatgc
     1741 tccgcatcat cgtggagttt ctcacaaagg tcggcctcga catcaagatc aagctcgtta
     1801 agcttctcca gcaggagcag agattgatgc tggatgaatt tagccatgtt cagcgctgtt
     1861 ttctgttgtt tcgtcataag aggcctcaga gcgtcagtgc tcagtgtagg gatgttctct
     1921 tttccgcttc ctcgctcact cagtcgctac gctcctgccg ttcggctgcg gcgagcggca
     1981 ggagcatgcg tatgaaagct cgagaaaacc gagcgatcac gtaatttaga aaagggaagg
     2041 ccgcacagcg gccgtttttc cataggctcc gcccccctga caagcatcac agaaacggac
     2101 gctcaaatca gtggtggcga aacccgacag gactataaag atatcaggcg tttccccctg
     2161 atggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt
     2221 tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc
     2281 aagctggact gtatgcacga accccccgtt cagtccgact gctgcgcctg ttccggtaac
     2341 tttcgacttg agtccaaccc ggaaggcacg acaaaacgcc actggcagca gccactggta
     2401 aggggtgtac aagaattact ggtgcagagt tcttgaagtt acagcctaac tacagctaaa
     2461 ctgaaaggac agatttggtg gcagcggtct tgtacaccct gttaccacgg ttcagaagtt
     2521 ccccaactta ctgatccttc gaaaaaccac ctccccaggt ggttttttcg tttacagagc
     2581 aaaagattac atcccgcagg ttaggatctc aagaagatcc ttttaatcgg aattcgacaa
     2641 ctccagataa gtcatggtga gccagccaac cccttcgact gaatatcgtt ccaaaaaaag
     2701 gcagtcagct gaaagaaact aacagtagaa caataatctc tagtaccctg aatctaagct
     2761 aacaacaaaa ctagattagg agttatagat ggcgatctat gccgtatgga ataacaaggg
     2821 tggtgtaggg aaaagttacc ttacttttca gttagcctct gaatatgcca gacagaaccc
     2881 tactaaaaag gtcctggtgg ttgacctgtg cccacaagcg aactcgtcct ctatgttact
     2941 tggtggaatg ataaatggcg aaaacacact aaaccagatt cattcagcaa tacagagaaa
     3001 gacaatatct ggatatattg aggataggat cagaagtcca tatattccta ccaacactgg
     3061 ttctagctat gtaataaatg tatctacaca caatagtaaa gttccaaata atctatatct
     3121 tgtcactggc gatgaacaat tagaactcca agcatcaaga gttgttgggg catcatttcc
     3181 aggtccccat gatgcatggc gtactgttca cctctggatt aatgatttaa tcagtgacat
     3241 ttgcaactca tggaataatg aagatagctg cgtattcatt gactgcaacc ctagcttctc
     3301 tatttataca gagttagctt taaccgcagc agagcgcctt ttaattcctt tctctgccga
     3361 tggttcatca aagagagcag ttaaggctgt actttcttta gtttatggtg agcgtcgcca
     3421 tcagaatgaa ccacaatcag atttctatct taatagcaat cgttattcta tgtcgctacc
     3481 acaaatatat atgtatgttg gaaatagatt aacgcaaatg aacaattcat ctgccagtgc
     3541 atttaagacg gtagtaaccg aaattggtga tgaaatttgg aaagtatggc aaacaaaacc
     3601 aagtctgttt tgtattcacc caaatgggag ctcaacgcca aataataaaa gatctttcag
     3661 gaatatgttt cagtatgaag taaatgatgc aaacacagca tctgtcgtat ctggagcctt
     3721 aggtattcca atcgcgacat taactgcagg aaataaaact gtttcaggaa aaataataaa
     3781 ggttaatcag acgcagctag ataaacaagt ccccaacatt gtagctctcg ctggtatgat
     3841 cgaataaaat agcaaggccc ttaaaagggc ctttttttat ttgtttttct ttactgcttg
     3901 cagcgccctg ccagcactag aggccaactc tttgattaca ttatctttac tttcaacaac
     3961 ctcagtaagc aactcgtttt cgttttccag tttattcaaa gcgttctgag tatcttcaat
     4021 agcatcatca tacccttcgg catagccatc catccaacca agagtatacg tttggtccaa
     4081 aagatcagct tgcttatcct gcagatcttt attctgatta ttgttagagc ccatgttttt
     4141 tcctcatttg ttggcgcatc tgagttttca aaccaggagg aattccattt ctaagtttct
     4201 cacttagtct gactggtggt gtgtttcctg agactggatt acagtcagca tccaccccga
     4261 catctttctt tttatagtcg ccaatattcc taacgttatc tgtagtcata gaaaaccacg
     4321 cccattgctg tgaataaccg aactataaac cagaaaaaaa acgtacggct tatcataaca
     4381 cttgacacaa aaaaacaccg ctactctcca agcaaaaaac ataaaattgg aaaaacaaaa
     4441 tcacaagcac atgataacaa tagatattaa tatttactta catttagtga gttttataat
     4501 tagaaaaaca atgtaattta ataatcacaa acattaccta aggacgaact gatgaaaata
     4561 cccgcgcatt tgaagcataa accaattatt caagtcgaaa attacgaccg cattgacggc
     4621 ccacatgccg atgacactga tgctatgggg ctatctgtag gtattgctca atggaacact
     4681 cctggatgga ctgagctttc ggcaaaggtt tggcgaaaca caggagaaaa atggtcaaga
     4741 caatcagaag agctgcctct tcaccgggta atcgaccttg caactctgat ctgtatcacc
     4801 atggactaca gtaaaaatgg gcgactttca tccagtgatg attttcccgt gactcgctca
     4861 actgataacc ctgagctagc acatcacact gagtttatga aaaaagagtt aaaacaaaat
     4921 aaaagcaatc ttgatgtaag cctcaaacgc ctctcgactg aattgaagag acttggctat
     4981 taaaactagc t
//
