LOCUS       NZ_CP079696             5251 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC31_S468MPCH
            plasmid pS468MPCH_P4, complete sequence.
ACCESSION   NZ_CP079696
VERSION     NZ_CP079696.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20130733
            Assembly: GCF_019317265.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC31_S468MPCH
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC31_S468MPCH
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 91.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:20:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,681
            CDSs (total)                      :: 5,555
            Genes (coding)                    :: 5,411
            CDSs (with protein)               :: 5,411
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 62 of 144
            Pseudo Genes (incomplete)         :: 78 of 144
            Pseudo Genes (internal stop)      :: 23 of 144
            Pseudo Genes (multiple problems)  :: 17 of 144
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079696.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC31_S468MPCH"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pS468MPCH_P4"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2014"
                     /collected_by="Christian medical college"
     gene            join(5166..5251,1..1270)
                     /locus_tag="KW564_RS28225"
                     /old_locus_tag="KW564_28200"
     CDS             join(5166..5251,1..1270)
                     /locus_tag="KW564_RS28225"
                     /old_locus_tag="KW564_28200"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_014386473.1"
                     /translation="MFVYLTIENFRSVKEKSTLNLSASGSSNHLTTHIYKNDGMNVGTL
                     MSAGIYGANASGKSNVLMAFEAIKFIATESGDLKEGKRIPCYEPYALSSATNEAPVKFE
                     AEFYSVEGNRFSYEVAFIKNRILFESLDYYPSRVKANLFTRDESDTWETIKFGGHYKGG
                     IKKIPFFPNNSYLAKAGNNAASPDIIKEAYNFFRKGIRHIGLNEKIRISSFGEREDVVN
                     ETAKVLCMVDTGVSGISVREVEAEIPIKMDEEIPQEIKEIIEEDYKYRYMFSHPTDEGG
                     SVSFPMGRESEGTQKLFEIIPLIRSAFNDSMVVIVDELDNSLHPHIADLIVKLFNDPDV
                     NKKGSQLVFSTHNMQLMAPEKMRRDQIWFCEKNKGASSLYSLDDFDKKKIKTTTPYAAW
                     YDEGRFGGVPDINYLKVASFISGDISLVMPDIDVKELSDGFFEEFDGDLSDE"
                     /name="ATP-binding protein"
     gene            1263..1883
                     /locus_tag="KW564_RS28230"
                     /old_locus_tag="KW564_28205"
     CDS             1263..1883
                     /locus_tag="KW564_RS28230"
                     /old_locus_tag="KW564_28205"
                     /inference="COORDINATES: protein motif:HMM:NF025084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="WP_023312615.1"
                     /translation="MSKKHGNNKKVPQVMYIYCEGEKTEPLYLEAYISDRSKRTLSVFK
                     IPKTRKNTPEQLVDEAIKKKNSSSSAEGDVFWVVYDQEHLTPSSISCHQKAWNKANRHG
                     INIAISCVCFELWLLLHFGFTTKDFSSYDNLMSSSPLKKLITNYDKGSKLTYLAIRERI
                     GTAKDNARKLDIYAKTNYKNTHEPYLFPCYTSFHELLKAIDDF"
                     /name="RloB family protein"
     gene            complement(2036..2197)
                     /locus_tag="KW564_RS28235"
                     /old_locus_tag="KW564_28210"
     CDS             complement(2036..2197)
                     /locus_tag="KW564_RS28235"
                     /old_locus_tag="KW564_28210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023144844.1"
                     /translation="MHEPPVQSDRCAFSGNYHLESNPERHGSSPLAAAIGNGLEEVSLE
                     VLRQLRLN"
                     /name="hypothetical protein"
     gene            2834..3154
                     /locus_tag="KW564_RS28240"
                     /old_locus_tag="KW564_28215"
     CDS             2834..3154
                     /locus_tag="KW564_RS28240"
                     /old_locus_tag="KW564_28215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_014386475.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3363..>3794
                     /locus_tag="KW564_RS28655"
                     /pseudo=""
     CDS             3363..>3794
                     /locus_tag="KW564_RS28655"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3832..4317
                     /locus_tag="KW564_RS28250"
                     /old_locus_tag="KW564_28225"
     CDS             3832..4317
                     /locus_tag="KW564_RS28250"
                     /old_locus_tag="KW564_28225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_013631087.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            4321..4533
                     /locus_tag="KW564_RS28255"
                     /old_locus_tag="KW564_28230"
     CDS             4321..4533
                     /locus_tag="KW564_RS28255"
                     /old_locus_tag="KW564_28230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          2548..2720
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.659"
                     /coverage="100.0"
                     /evalue="9.74e-41"
     Typing          complement(2085..2601)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.462"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3145..4649
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.215"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttaacaact catatctata aaaatgatgg aatgaatgtt ggaacgctca tgagtgctgg
       61 gatttatggt gctaatgcgt ctggtaaatc taacgttctc atggcttttg aagctataaa
      121 atttatcgca acagaaagcg gtgatttgaa agaaggtaaa cggattccct gctatgagcc
      181 atatgctctt tcttcggcta cgaatgaggc tcctgtaaag tttgaggctg aattttattc
      241 tgttgagggg aatcggttca gttatgaagt tgcttttatt aaaaatcgga ttttatttga
      301 atcattagac tattatccat ctagggttaa agcaaattta tttactagag atgagagcga
      361 cacctgggaa acaatcaagt ttggtgggca ttataaagga ggtattaaaa aaataccatt
      421 cttccctaat aattcatact tagcgaaagc aggtaataat gccgcatcac cagacataat
      481 taaagaagct tataactttt ttagaaaggg aattagacat ataggtttaa atgaaaagat
      541 aagaatatct tcttttgggg agagggagga cgtcgtaaat gaaacggcca aggttttatg
      601 catggttgac actggtgttt ccggtatatc tgtacgggag gttgaggcgg aaataccaat
      661 aaaaatggat gaggaaatcc ctcaagaaat aaaggagata attgaagaag attataaata
      721 tcgttatatg ttctctcatc ctactgatga aggggggagt gtttctttcc ctatggggag
      781 agagtctgaa ggcacccaaa aattatttga aatcatacct ctaattagat ctgcgtttaa
      841 tgatagtatg gttgtaattg ttgatgagtt agataatagc ttgcacccgc atattgctga
      901 cttgattgtt aagttattta atgatcctga tgtcaataaa aaaggctcac aacttgtatt
      961 ctcaacccat aatatgcagt taatggctcc agagaaaatg agaagagatc agatatggtt
     1021 ttgtgaaaaa aataaaggtg cgtcgtctct ttatagctta gatgattttg ataagaaaaa
     1081 gataaaaaca acaacacctt acgcagcttg gtatgatgag ggtaggtttg gtggggttcc
     1141 cgacataaac tacttaaagg tagcctcatt tatatctggt gatatatcct tggtaatgcc
     1201 ggatattgac gtcaaagaat tgtctgatgg tttttttgaa gagtttgacg gggatctttc
     1261 tgatgagtaa aaaacatgga aataataaga aagtcccaca agtaatgtat atatactgtg
     1321 agggtgagaa aaccgaacca ttataccttg aggcttatat ttcagacaga tctaaaagaa
     1381 ctttaagtgt atttaaaata cctaaaacaa ggaaaaatac acctgagcag ttggtggatg
     1441 aggcgataaa gaaaaagaat tcatcatctt ccgcagaagg tgacgtcttt tgggttgttt
     1501 atgatcaaga gcatctgacg ccttcgtcta tttcatgtca tcaaaaggca tggaataaag
     1561 ctaataggca tgggataaat atagcaatat cctgtgtttg ttttgaatta tggttattgt
     1621 tgcatttcgg cttcacaacc aaagattttt cgtcgtatga taatttgatg tctagctccc
     1681 cgctaaaaaa attaataact aactatgata aaggtagtaa actaacttac cttgctataa
     1741 gagagaggat tggtactgcg aaagataacg caagaaagct agatatttac gctaaaacta
     1801 attataaaaa cacgcacgaa ccttatctat ttccatgtta tacatcattc catgagctac
     1861 ttaaggcgat tgacgacttt tgaagtaatc cattgaccat ggcttattac aggctgtaat
     1921 ctatgaaaaa taaaaaccac cgctaccaac ggtggttttt ggaaggtaca tgagctacca
     1981 acttatgtac cgggtaactg gcttagaggg agcgcagtca ccaaaaattg tcctttcagt
     2041 ttagccttaa ctggcgcaga acttcaagac tcacttcctc taatccgtta ccaatggctg
     2101 ctgccagtgg cgatgaaccg tgtctttccg ggttggactc aagatgatag ttaccggaga
     2161 aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg
     2221 cctacccgga actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg
     2281 acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccacc agggggaaac
     2341 gcctggtatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg
     2401 tgatgcttgt caggggggcg gagcctatgg aaaaacggca gcggcgcatc ctttttctgc
     2461 cccgtttgtc atgccatatg ccttcgctct tctccgtgtg caggtaacgc ccgccgcagc
     2521 cgaacggcag gagcggcagc gactgagtga gcgaggaggc gtcatatcct ctgaacttcc
     2581 attttctgcc ttacgcccgc agctgcattt tcctctgtca catgaagcac tttcctgatt
     2641 accgcactca tgccaacata ataagccagt atacactccg ctagcgctac gtgactggtt
     2701 cagggctgcg ccccgaaacc cgctaaaagc gactgacgcg cctgcggctt gtccaacacc
     2761 gtgccgaccg ggaaacattt cccgcccgtc ccggtgttat caggaggcta ctctggcaga
     2821 caaacgcaac aagatgctca cgatgtgggt gacggaagac gaacaccggc ggctgctcga
     2881 gcggtgcgac ggcaagcagc tggcggcgtg gatgcgtcag acctgcctcg atgagaagcc
     2941 ggcgcgtgcc ggcagactgc cgtcgatatc gccggcgctg ctgcgccagc ttgccggtat
     3001 ggggaacaac ctgaaccaga tcgcccggaa agtgaatgcc ggtggtgccg gtcatgaccg
     3061 ggtgcagatt gtggccgcac tgatggcaat cgacgcggga ctggagcggc tgcggcatgc
     3121 ggtgctggaa aaaggggggg atgatgatcg ttaaatttca cccccggggg cgcggtggtg
     3181 gtgccggtcc ggtggattac ctgctgggaa aagaccggca gcgcgagggg gcgaccgtgc
     3241 tgcagggaaa accggaggaa gtccgggagc ttatcgatgc ctcgccgtat gcgaaaaagt
     3301 acacctccgg cgtgctgtcg ttcgcggagg ctgaactgcc gcccgggcag cgggagcaga
     3361 tcatggccag ctttgagcgg gttctgatgc ccgggctcga taaagaccag tacagcattc
     3421 tgtgggtgga gcacaccgac aaggggcggc tggagctgaa tttcctgatc cccaacactg
     3481 aactgctgac cgggaaacgc ctgcagccgt actacgatcg cgccgaccgg ccgcgcatcg
     3541 atgcctggca gaccgtggtg aacggcaggc tggggctgca tgatccaaac gccccggaga
     3601 accgacggtt actggtcacg ccgtccgcac tgccggaaac gaagctggaa gccgctcagg
     3661 cgattacacg tgggttgctg gccttagcct catccgggga gctcaaaacg cgcgaggacg
     3721 tcacaggggc gctcacagcg gcaggttttg aggtggtccg caccacaaaa aacagtatca
     3781 gcattgccga cccggacggg gggcgaaaca tccgactgaa gggagccatt tatgagcagt
     3841 cttttagcgc tggcgaagga cttggagcag caatcgaaag cgcagcagca gagtaccggc
     3901 gagatgctga aagccgcatt cagcgagcac gagcagtctg taaaagcgga actgagcgca
     3961 agcgcgaaga gaatcagcga cgccatcagc gcccacgagc agggtatgac cgcggcgatg
     4021 cagtcgaacc ggctgagcgt gctgcgtatg gtcggccgga cgtggctgac catcaccctg
     4081 gtctcggtac tgctgatcgc cacgagcggg agcattctgt ggtggcaggg gcagcagatc
     4141 accgggaatt accagactat ccgggcgcag gagcggacgc aggccatgct gtcggagaag
     4201 aaccacggcg tgcagctgtc gctctgcggg gagaagaaac tgtcctgcgt caaagtgaac
     4261 ccgaaagcgg gagcttacgg ggaggaaggg aactggatgg tactggagag gaaatagccc
     4321 atgacggaac tggaaaaaca gttgctgacc gcattagagc agctacatca ggactactcg
     4381 caaaggctgg acgagtggga gagcgccttc gcggaatggc agaagatgtc tggtcttatg
     4441 caacgggaga acgcggcgct gaacgagcgc gtcacgcgct tgagtcagca ggtggcgaac
     4501 ttgagcaggc agttacagcg tttgagccag tagtgcagcg gcatgaaatg gcggtggccg
     4561 ccgaacgggc atacgagcag aaacagcatg aaaaagagct ggcggaaacc cgttcccggc
     4621 agcggagcta tgacggtccg acgctgggct gactgcgcgc atccagtgac aaaccccaag
     4681 aagaagggct cataaatttc ccgctaaata cggtcgtgat gtttccatca cagcgtatac
     4741 aacggaggga ttatgagact gtttaacgtg attgagacgg tgctgaattg cctgtacatg
     4801 gcgctggaga tctgggactg gctggagaag cattttcctg tttttaaggc gttcatccgg
     4861 attctggcct gaaatcagac cgtatgcatc tggctgcatg accatgcagc cgggtaaaat
     4921 ttatctgtca cgaacggggc ggtgtatcgg gtggtttgtt gccggtttta cctgactgcc
     4981 gccccgtttg tggcgaacct gtccggagcg gtgcgggcaa cagatgttat ggtaaatagc
     5041 gtgtaattat aaatagcaca acgtttttta aggaagatgt tgttccaaaa ttaaaacata
     5101 taggatatct ttcctatgat tgatttttcg cttttttggc gaaaaatccc cgttaggagg
     5161 tatgcatgtt tgtttatttg actattgaaa attttaggtc ggttaaggaa aaatcgacat
     5221 taaacttgag cgcatccggt tctagtaacc a
//
