LOCUS       NZ_CP079688             3998 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC30_AI1547MPCH
            plasmid pAI1547MPCH_P5, complete sequence.
ACCESSION   NZ_CP079688
VERSION     NZ_CP079688.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20130732
            Assembly: GCF_019317245.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC30_AI1547MPCH
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC30_AI1547MPCH
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 91.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:21:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,370
            Genes (coding)                    :: 5,215
            CDSs (with protein)               :: 5,215
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 69 of 155
            Pseudo Genes (incomplete)         :: 87 of 155
            Pseudo Genes (internal stop)      :: 21 of 155
            Pseudo Genes (multiple problems)  :: 20 of 155
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079688.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC30_AI1547MPCH"
                     /isolation_source="Pus"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pAI1547MPCH_P5"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2016"
                     /collected_by="Christian medical college"
     gene            169..1023
                     /locus_tag="KW563_RS27330"
                     /old_locus_tag="KW563_27305"
     CDS             169..1023
                     /locus_tag="KW563_RS27330"
                     /old_locus_tag="KW563_27305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071579761.1"
                     /translation="MIIQEEHNIDFFKNQMPNKPYCTNNLDMGLSIRNKAKALEMLYLQ
                     ANQPAIQTCLLFDLDKKNSFYTFEQVGLPIPHFITKTPKTGRCHYGYMLKAGVCKTQQA
                     RLKPLKYAAAVEMAMAKKLKADLGFAGLITKNPLNDHWSPFWSGADLYDLDYLADFVDL
                     EKPQIQKKKSEAYGLGRNVNLFEDLRQYAYRTVLNFKKISTFEKFENELLLKAQGLNTF
                     CNPQNPLQYKEIKATVRSVARWTWKNFDSHTFSQIQSNRAKKPRKTNVKNEMLDILMSK
                     GVL"
                     /name="replication initiation protein"
     gene            1020..1283
                     /locus_tag="KW563_RS27335"
                     /old_locus_tag="KW563_27310"
     CDS             1020..1283
                     /locus_tag="KW563_RS27335"
                     /old_locus_tag="KW563_27310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045287009.1"
                     /translation="MIINGKKIKAKEIMEMTGRSERTVRKYFSQSRDDYEKTAMDRRRQ
                     AYELRSQGLKWQQVADKMGCSYHGAVALYRRYVALDMPQNSL"
                     /name="hypothetical protein"
     gene            complement(1522..1806)
                     /locus_tag="KW563_RS27340"
                     /old_locus_tag="KW563_27315"
     CDS             complement(1522..1806)
                     /locus_tag="KW563_RS27340"
                     /old_locus_tag="KW563_27315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_071579762.1"
                     /translation="MSKTTAQQIADLQNKLQALRTKKARQDRREETRQKIILGAEVAKV
                     LGKKPEEINKALILGLLSKINELDEEEKQEFTRLGLNILEEWKNENRKP"
                     /name="conjugal transfer protein TraD"
     gene            complement(1810..2193)
                     /locus_tag="KW563_RS27345"
                     /old_locus_tag="KW563_27320"
     CDS             complement(1810..2193)
                     /locus_tag="KW563_RS27345"
                     /old_locus_tag="KW563_27320"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071579763.1"
                     /translation="MNKLITEAEGAKIESVFKYILEASESILIEHGHVITGQIIKNKKF
                     FADNGHKDFERLEYYLSSAISSLVEDKVTEGIKDINTDENLVFKKINELSDKEKLILAE
                     CIIKHFEYSNVRGMQKAFNIFGG"
                     /name="hypothetical protein"
     gene            2437..3630
                     /gene="mobQ"
                     /locus_tag="KW563_RS27350"
                     /old_locus_tag="KW563_27325"
     CDS             2437..3630
                     /gene="mobQ"
                     /locus_tag="KW563_RS27350"
                     /old_locus_tag="KW563_27325"
                     /inference="COORDINATES: protein motif:HMM:NF041496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_076997031.1"
                     /translation="MATFHISFKKLRRSEGKSSVYLSAYQNREKTKDNRTGATWDYSKK
                     EGFFGSAILSPAGTPAELVKDSGTLWNAVEAGEKRKDAELCRYLDIAIPKELDDGQKKQ
                     IVLDYCQENFVDYGMIADIAFHDLDSHNPHAHVMLTLRTVSPNGFGNKQREWNNRENIE
                     AWRVNWEIIANDRLKKYGHKSRIDSRSLKDQKEEAERKAVFAKTPKEKAKWISKTIELD
                     RTPMTRIHRHQWRKGQKLRAMEQKEKALLLKIANATYLQQTKPPQPEETMKIEKKPLAR
                     SIIDNLKALYKKTQHIFKSKPNVNKNKKEEKEDPQNFVSSYVINEFGEHILKTELETKP
                     KTGTGTGTGGNNGGGKMGSGSGMSPMNQPQPKIEEPEPEPMPEFEDQQPKRKKKFGM"
                     /name="mobQ"
     Typing          2438..3447
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="85.0"
                     /evalue="0.0"
ORIGIN
        1 tcgttgggct gttatctgat atggttagac gcacacttga caaatttttg agatcggtta
       61 gaactggtac aggattgaaa aatctataaa aataaaaacc ccaccctctg cgattttgtt
      121 ttggcgacag aaaacagaga gtagggccag ataaaaagga ctgaaaaaat gataatacaa
      181 gaagaacata acatcgattt ttttaaaaat caaatgccta acaagcctta ttgtacaaat
      241 aacctcgata tgggattaag cataaggaac aaggcaaaag cccttgaaat gctgtattta
      301 caggcaaatc agccagccat tcaaacttgc ttactctttg atctagataa gaaaaattca
      361 ttctatacat ttgagcaggt gggcttaccc atacctcact ttattacaaa aacaccaaaa
      421 acaggtcgtt gtcactacgg ctatatgctg aaagccgggg tttgtaagac acaacaagca
      481 aggttaaagc cgttgaagta tgccgcagcc gttgaaatgg ctatggcgaa aaaactaaaa
      541 gcagatctgg ggtttgctgg attgattaca aaaaacccac tcaatgatca ctggtcgccg
      601 ttctggtcag gtgctgacct ttatgacctg gattatttag ctgattttgt ggacttagaa
      661 aagcctcaaa tacaaaagaa aaaaagcgag gcttatggtt taggtcgcaa tgttaatttg
      721 tttgaagatc tacggcaata tgcttacaga acggttttaa atttcaaaaa aatatccact
      781 ttcgagaaat tcgaaaacga actactatta aaggcacagg gcttaaacac tttttgcaat
      841 ccacaaaacc cgttacaata caaagagata aaagcaacag ttagaagtgt agcccgttgg
      901 acttggaaaa attttgatag ccatacattt tcacaaatcc aatcaaacag agccaaaaaa
      961 cctcgaaaaa ccaatgttaa aaatgaaatg ttagacatat taatgagcaa gggggtttta
     1021 tgatcattaa tggtaaaaaa ataaaagcaa aagaaataat ggaaatgaca ggtcgtagtg
     1081 aaagaactgt aagaaaatat ttttctcaat ctcgtgacga ttacgaaaaa acagcaatgg
     1141 atcgtcgtcg tcaggcttac gaactacgaa gccaggggct gaaatggcaa caagtagccg
     1201 acaaaatggg ctgttcgtat cacggagccg ttgccctgta tcgccgttat gttgccttag
     1261 atatgcctca aaacagcctg taacgacttc taagcagttt ttccccctaa cctaaccaaa
     1321 ccctccaccc ctggggggat tttttcccct ttccccgtat ccgggccctg tatctcgaac
     1381 tgagaggcac tcaggtaccc aacaatcaac aaacccaccc ataagccctg agagcctctc
     1441 tgagcctttt taagacctcc aggcaagcct ggcaaccctt cacagtaaaa aaccagccaa
     1501 gatcggctgg ttttttattg ttcacggttt acgattttca ttcttccact cttctaaaat
     1561 atttaatcct aatcgtgtga attcttgttt ttcttcttcg tcaagttcat ttattttact
     1621 taacaaccca agaattaaag ccttgttaat ttcctccggt tttttaccta aaaccttagc
     1681 cacttctgca cccaggatta ttttttgtct tgtttcttcc cttctgtcct gtcttgcttt
     1741 ttttgttctt aatgcttgta gtttattttg caaatctgcg atctgctgtg ctgtcgtttt
     1801 actcatccat taccccccaa aaatattaaa tgccttttgc attcctctaa catttgaata
     1861 ttcaaaatgt ttaataatgc actctgctaa aattaatttt tccttgtcac tcaattcatt
     1921 tattttttta aaaacaaggt tctcatccgt gttaatgtct ttaatgcctt ctgttacctt
     1981 atcctcgaca agagaactaa ttgccgagct aaggtaatac tctaaccttt caaaatcttt
     2041 atgcccgtta tcagcaaaga actttttgtt ttttattatt tgccccgtaa taacatgccc
     2101 atgctctatt aatatgcttt cagatgcctc taatatgtat ttaaaaacac tttcgatttt
     2161 tgcaccttct gcctctgtta taagtttatt cataataacc tcctaattat ttattttgtt
     2221 atttattacc tccattattt actttagtca aatatatttt agaataaaac aaataatatt
     2281 taatattggg cgaagcctgg ggttcattac ttattatttt gaggtgcaac cgaaaaaata
     2341 atattagtaa tgcccactta cacgtcgctt gcgacgtatg ggccctgcgg ggctaaaaac
     2401 caaaattcaa aagacaaaaa aagtaagggg tagactatgg cgacatttca tatcagcttt
     2461 aaaaaattac gaagatcaga aggtaaatct tcggtttatt tatctgcata tcaaaaccga
     2521 gaaaagacaa aagataatcg aaccggggca acctgggatt attcaaaaaa agagggattt
     2581 ttcggctctg ctatcctctc ccctgccggc acacctgccg aactggtgaa ggactcaggc
     2641 accctctgga atgccgtaga ggctggcgag aagcgaaagg atgccgaatt atgccgttat
     2701 ctggacatag ccatccccaa ggagctggac gacggccaga agaagcagat cgtcctcgat
     2761 tactgccaag aaaatttcgt ggattatgga atgattgcag acatagcatt tcatgatctt
     2821 gatagtcata accctcatgc tcatgtaatg ctaactttaa gaactgttag cccaaatgga
     2881 tttgggaaca aacaaagaga atggaataat agagaaaaca ttgaagcctg gcgagttaac
     2941 tgggaaataa tagcaaatga ccgattaaaa aaatatggtc ataaatctcg tattgactcc
     3001 cgttcattaa aagaccaaaa agaagaggca gaaagaaagg ctgtttttgc taaaactccg
     3061 aaagagaaag ccaagtggat ttcaaaaact attgaactgg acagaacgcc aatgaccagg
     3121 atacacaggc accaatggcg aaaaggtcaa aaattaagag ctatggagca aaaagaaaaa
     3181 gcattattat taaaaatagc taatgcaaca tacttacagc aaaccaaacc acctcagcca
     3241 gaggaaacta tgaaaataga gaaaaagcct cttgcaaggt caatcattga taatttaaag
     3301 gcattataca aaaagaccca gcatatattt aaatcaaaac ctaatgtaaa taaaaataaa
     3361 aaagaagaaa aagaagatcc tcaaaatttc gttagtagtt atgtaataaa tgaatttggc
     3421 gaacatatat taaagacaga gttagaaaca aaacctaaaa caggcacagg aaccggaacc
     3481 ggtggaaata atggaggcgg aaaaatgggt tcaggctctg gaatgtctcc tatgaaccaa
     3541 ccacaaccaa aaattgaaga gcctgagccg gaaccaatgc cggaattcga agatcagcaa
     3601 cctaagagga agaagaaatt tggaatgtag tatatagtgg tatatattcc tatatagtag
     3661 tatcacttaa tacaacttaa tattatttaa tactatacta taagatacta catttttaga
     3721 gttgtgatcc tcgccggttc tatttcccct ttatgtattg ccacagcttc attaactgac
     3781 ttcataagca ttttaaaaaa tttactatgc ttatttcttt tccgtttttt ctgcactttc
     3841 ttttttttca tatattcacc aaaaaaaatc gaatcgccaa tttttcttcg aacaatactc
     3901 ttttttcttt ttttattgtt tttctatctt cggtaatgct tcgcaccata gatctgatag
     3961 taatgctttg ctgccgtatg taaagaactg ccccaact
//
