LOCUS       NZ_CP079687             4991 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC30_AI1547MPCH
            plasmid pAI1547MPCH_P4, complete sequence.
ACCESSION   NZ_CP079687
VERSION     NZ_CP079687.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20130732
            Assembly: GCF_019317245.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC30_AI1547MPCH
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC30_AI1547MPCH
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 91.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:21:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,370
            Genes (coding)                    :: 5,215
            CDSs (with protein)               :: 5,215
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 69 of 155
            Pseudo Genes (incomplete)         :: 87 of 155
            Pseudo Genes (internal stop)      :: 21 of 155
            Pseudo Genes (multiple problems)  :: 20 of 155
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079687.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC30_AI1547MPCH"
                     /isolation_source="Pus"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pAI1547MPCH_P4"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2016"
                     /collected_by="Christian medical college"
     gene            complement(join(4298..4991,1..365))
                     /locus_tag="KW563_RS27300"
                     /old_locus_tag="KW563_27275"
     CDS             complement(join(4298..4991,1..365))
                     /locus_tag="KW563_RS27300"
                     /old_locus_tag="KW563_27275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508929.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_042109829.1"
                     /translation="MAIYAVWNNKGGVGKSYLTFQLASEYARQNPTKKVLVVDLCPQAN
                     SSSMLLGGMINGENTLNQIHSAIQRKTISGYIEDRIRSPYIPTNTGSSYVINVSTHNSK
                     VPNNLYLVTGDEQLELQASRVVGASFPGPHDAWRTVHLWINDLISDICNSWNNEDSCVF
                     IDCNPSFSIYTELALTAAERLLIPFSADGSSKRAVKAVLSLVYGERRHQNEPQSDFYLN
                     SNRYSMSLPQIYMYVGNRLTQMNNSSASAFKTVVTEIGDEIWKVWQTKPSLFCIHPNGS
                     STPNNKRSFRNMFQYEVNDANTASVVSGALGIPIATLTAGNKTVSGKIIKVNQTQLDKQ
                     VPNIVALAGMIE"
                     /name="ParA family protein"
     gene            complement(695..861)
                     /locus_tag="KW563_RS27785"
                     /pseudo=""
     CDS             complement(695..861)
                     /locus_tag="KW563_RS27785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            1277..1468
                     /locus_tag="KW563_RS27305"
                     /old_locus_tag="KW563_27280"
     CDS             1277..1468
                     /locus_tag="KW563_RS27305"
                     /old_locus_tag="KW563_27280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032489493.1"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1468..1869
                     /locus_tag="KW563_RS27310"
                     /old_locus_tag="KW563_27285"
     CDS             1468..1869
                     /locus_tag="KW563_RS27310"
                     /old_locus_tag="KW563_27285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032489494.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQLKGEHHSGQVSEQRDTASVDQCEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            2664..3068
                     /locus_tag="KW563_RS27315"
                     /old_locus_tag="KW563_27290"
     CDS             2664..3068
                     /locus_tag="KW563_RS27315"
                     /old_locus_tag="KW563_27290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="WP_008321396.1"
                     /translation="MTILVTIQAQLVVGEEQVIKSLAPEGMLAAVFEDDGRTGYFYALD
                     ESVEGNPIMDAVHIYNAEDVSDGHIPSDVKIGWSEDSQKCVLLINGYPHGAFDFVGKNG
                     YCRSGYPPPINKVWSLSGHEWSDSVDDFFR"
                     /name="DUF2251 domain-containing protein"
     gene            complement(3162..3593)
                     /locus_tag="KW563_RS27320"
                     /old_locus_tag="KW563_27295"
     CDS             complement(3162..3593)
                     /locus_tag="KW563_RS27320"
                     /old_locus_tag="KW563_27295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_042109831.1"
                     /translation="MKIPAHLKHKPIIQVENYDRIDGPHADDTDAMGLSVGIAQWNTPG
                     WTELSAKVWRNTGEKWSRQSEELPLHRVIDLATLICITMDYSKNGRLSSSDDFPVTRST
                     DNPELAHHTEFMKKELKQNKSNLDVSLKRLSTELKRLGY"
                     /name="DUF6530 family protein"
     gene            4011..4268
                     /locus_tag="KW563_RS27325"
                     /old_locus_tag="KW563_27300"
     CDS             4011..4268
                     /locus_tag="KW563_RS27325"
                     /old_locus_tag="KW563_27300"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042109830.1"
                     /translation="MGSNNNQNKDLQDKQADLLDQTYTLGWMDGYAEGYDDAIEDTQNA
                     LNKLENENELLTEVVESKDNVIKELASSAGRALQAVKKNK"
                     /name="hypothetical protein"
     Typing          complement(754..1156)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.857"
                     /coverage="99.0"
                     /evalue="7.72e-165"
ORIGIN
        1 gcttggagtt ctaattgttc atcgccagtg acaagatata gattatttgg aactttacta
       61 ttgtgtgtag atacatttat tacatagcta gaaccagtgt tggtaggaat atatggactt
      121 ctgatcctat cctcaatata tccagatatt gtctttctct gtattgctga atgaatctgg
      181 tttagtgtgt tttcgccatt tatcattcca ccaagtaaca tagaggacga gttcgcttgt
      241 gggcacaggt caaccaccag gaccttttta gtagggttct gtctggcata ttcagaggct
      301 aactgaaaag taaggtaact tttccctaca ccacccttgt tattccatac ggcatagatc
      361 gccatctata actcctaatc tagttttgtt gttagcttag attcagggta ctagagatta
      421 ttgttctact gttagtttct ttcagctgac tgcctttttt tggaacgata ttcagtcgaa
      481 ggggttggct ggctcaccat gacttatctg gagttgtcga attccgatta aaaggatctt
      541 cttgagatcc taacctgcgg gatgtaatct tttgctctgt aaacgaaaaa accacctggg
      601 gaggtggttt ttcgaaggat cagtaagttg gggaacttct gaaccgtggt aacagggtgt
      661 acaagaccgc tgccaccaaa tctgtccttt cagtttagct gtagttaggc tgtaacttca
      721 agaactctgc accagtaatt cttgtacacc ccttaccagt ggctgctgcc agtggcgttt
      781 tgtcgtgcct tccgggttgg actcaagtcg aaagttaccg gaacaggcgc agcagtcgga
      841 ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgcctacc cggaactgag
      901 tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa
      961 aggcaggaac aggagagcgc acgagggagc catcaggggg aaacgcctga tatctttata
     1021 gtcctgtcgg gtttcgccac cactgatttg agcgtccgtt tctgtgatgc ttgtcagggg
     1081 ggcggagcct atggaaaaac ggccgctgtg cggccttccc ttttctaaat tacgtgatcg
     1141 ctcggttttc tcgagctttc atacgcatgc tcctgccgct cgccgcagcc gaacggcagg
     1201 agcgtagcga ctgagtgagc gaggaagcgg aaaagagaac atccctacac tgagcactga
     1261 cgctctgagg cctcttatga cgaaacaaca gaaaacagcg ctgaacatgg ctaaattcat
     1321 ccagcatcaa tctctgctcc tgctggagaa gcttaacgag cttgatcttg atgtcgaggc
     1381 cgacctttgt gagaaactcc acgatgatgc ggagcatctt ttccgtacac tgtcctcgag
     1441 actggatgca ttacaggatg gtaactgatg gccagagtgt ctattagtga ggcagcccga
     1501 ctgacgggta agagtagaac aacactgcac aggttaatta aagcaggtga cctgtccacc
     1561 tgtagcggtg agcgcaatgc aaaaatggta gatacttcag agcttttacg tgtctttggt
     1621 ccctttgaac aactgaaggg tgaacaccat tctggacagg tctctgaaca gcgcgataca
     1681 gcctctgttg accagtgtga acaggtgatt cgtcatctga aacaggagat tgaacacctg
     1741 aacactttac ttttgtccaa agattcgcac attgacagcc tgaaacaggc catgctcctt
     1801 cttgaaaatc accaggcaaa attaactgaa tccccggcgc catggtggaa attctggaaa
     1861 aaatcctgac cctgtcatgc ttcagttttc ctctccggcc atcccgggga gttcctgaac
     1921 cttcccaaaa atagtcagat ctctgaacgc tcctcaccac taaaaatacc cacaccccac
     1981 gcaaaattaa aattttgcag atttgtattt aatatcagta gtttactttc ctgagatttc
     2041 gttttttaaa ttttatttat atagatccga ctctatgacg actcgtcata gagtccccgg
     2101 cgagtcgtca tagagtcggt aacgactcag ggctataaaa acaatgagtt aataacgacc
     2161 cgactctaga cccgactccc cgtttggttg ttttttagtc gcggcgactc taggtacgac
     2221 tctagtgcag agtcgccggt gaatcgtgtg gtgagtcgtc cggcgacttg ccataacgtg
     2281 attgccggcc gtcgacgaac ccgattcctg aacgtgctga aaaccggttt tccggctgcg
     2341 ccggcaacgc ttcacggcca cgcaataaat tgcgtccccg ttcaccgttt tcggatggga
     2401 agaacgccgg aaacaggact taccctgtct gaatggcgtg aatattcgcg ctatgcactt
     2461 gcgtgcatac tcatgcatgc cataaaaaca gagcctgcgc gtttctggcg ggttttcggg
     2521 tggtttgttg cctgttttac cggtttcccg tcagaaacgc cctcagcgcg tttctgacga
     2581 tgctgataac ggatgttatg gtaaattatc cgcttgtctg aaagctggct gccgaagcaa
     2641 ccaaggaaag gagaacagac ctgatgacca ttttagtgac gatacaggcc cagctcgttg
     2701 tcggggagga acaggttata aagagcctcg ctcctgaggg catgctggcc gccgtgttcg
     2761 aagacgatgg ccggaccggg tatttctatg cgctcgatga gtccgtcgag ggaaacccga
     2821 ttatggacgc agtacacatc tacaatgccg aagacgtttc agacggtcac attccttctg
     2881 atgttaaaat tggctggtca gaagacagtc agaagtgcgt actcctgatc aatggatacc
     2941 cgcacggggc attcgatttc gtgggaaaaa acgggtactg caggagtggt tatcctcccc
     3001 ctataaacaa ggtgtggtct ctttccgggc atgagtggag cgactctgtg gatgattttt
     3061 tccggtgaat tacggcaggc tatagcctgc cattatggtt cgaaaaacgg atgttatggt
     3121 aaattcgtct gtgaagtagg tagacatttc taaagctagt tttaatagcc aagtctcttc
     3181 aattcagtcg agaggcgttt gaggcttaca tcaagattgc ttttattttg ttttaactct
     3241 tttttcataa actcagtgtg atgtgctagc tcagggttat cagttgagcg agtcacggga
     3301 aaatcatcac tggatgaaag tcgcccattt ttactgtagt ccatggtgat acagatcaga
     3361 gttgcaaggt cgattacccg gtgaagaggc agctcttctg attgtcttga ccatttttct
     3421 cctgtgtttc gccaaacctt tgccgaaagc tcagtccatc caggagtgtt ccattgagca
     3481 atacctacag atagccccat agcatcagtg tcatcggcat gtgggccgtc aatgcggtcg
     3541 taattttcga cttgaataat tggtttatgc ttcaaatgcg cgggtatttt catcagttcg
     3601 tccttaggta atgtttgtga ttattaaatt acattgtttt tctaattata aaactcacta
     3661 aatgtaagta aatattaata tctattgtta tcatgtgctt gtgattttgt ttttccaatt
     3721 ttatgttttt tgcttggaga gtagcggtgt ttttttgtgt caagtgttat gataagccgt
     3781 acgttttttt tctggtttat agttcggtta ttcacagcaa tgggcgtggt tttctatgac
     3841 tacagataac gttaggaata ttggcgacta taaaaagaaa gatgtcgggg tggatgctga
     3901 ctgtaatcca gtctcaggaa acacaccacc agtcagacta agtgagaaac ttagaaatgg
     3961 aattcctcct ggtttgaaaa ctcagatgcg ccaacaaatg aggaaaaaac atgggctcta
     4021 acaataatca gaataaagat ctgcaggata agcaagctga tcttttggac caaacgtata
     4081 ctcttggttg gatggatggc tatgccgaag ggtatgatga tgctattgaa gatactcaga
     4141 acgctttgaa taaactggaa aacgaaaacg agttgcttac tgaggttgtt gaaagtaaag
     4201 ataatgtaat caaagagttg gcctctagtg ctggcagggc gctgcaagca gtaaagaaaa
     4261 acaaataaaa aaaggccctt ttaagggcct tgctatttta ttcgatcata ccagcgagag
     4321 ctacaatgtt ggggacttgt ttatctagct gcgtctgatt aacctttatt atttttcctg
     4381 aaacagtttt atttcctgca gttaatgtcg cgattggaat acctaaggct ccagatacga
     4441 cagatgctgt gtttgcatca tttacttcat actgaaacat attcctgaaa gatcttttat
     4501 tatttggcgt tgagctccca tttgggtgaa tacaaaacag acttggtttt gtttgccata
     4561 ctttccaaat ttcatcacca atttcggtta ctaccgtctt aaatgcactg gcagatgaat
     4621 tgttcatttg cgttaatcta tttccaacat acatatatat ttgtggtagc gacatagaat
     4681 aacgattgct attaagatag aaatctgatt gtggttcatt ctgatggcga cgctcaccat
     4741 aaactaaaga aagtacagcc ttaactgctc tctttgatga accatcggca gagaaaggaa
     4801 ttaaaaggcg ctctgctgcg gttaaagcta actctgtata aatagagaag ctagggttgc
     4861 agtcaatgaa tacgcagcta tcttcattat tccatgagtt gcaaatgtca ctgattaaat
     4921 cattaatcca gaggtgaaca gtacgccatg catcatgggg acctggaaat gatgccccaa
     4981 caactcttga t
//
