LOCUS       NZ_CP079677             9730 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC29_CMC309MPCH
            plasmid pCMC309MPCH_P4, complete sequence.
ACCESSION   NZ_CP079677
VERSION     NZ_CP079677.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20130677
            Assembly: GCF_019317225.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC29_CMC309MPCH
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC29_CMC309MPCH
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 64.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:10:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,422
            CDSs (total)                      :: 5,303
            Genes (coding)                    :: 5,153
            CDSs (with protein)               :: 5,153
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 67 of 150
            Pseudo Genes (incomplete)         :: 82 of 150
            Pseudo Genes (internal stop)      :: 29 of 150
            Pseudo Genes (multiple problems)  :: 23 of 150
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079677.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC29_CMC309MPCH"
                     /isolation_source="Endotracheal aspirate"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pCMC309MPCH_P4"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2018"
                     /collected_by="Christian medical college"
     gene            join(9611..9730,1..1842)
                     /locus_tag="KW562_RS26815"
                     /old_locus_tag="KW562_26800"
     CDS             join(9611..9730,1..1842)
                     /locus_tag="KW562_RS26815"
                     /old_locus_tag="KW562_26800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_063938523.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            1842..2573
                     /gene="mobC"
                     /locus_tag="KW562_RS26820"
                     /old_locus_tag="KW562_26805"
     CDS             1842..2573
                     /gene="mobC"
                     /locus_tag="KW562_RS26820"
                     /old_locus_tag="KW562_26805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071653262.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            2580..3110
                     /locus_tag="KW562_RS26825"
                     /old_locus_tag="KW562_26810"
     CDS             2580..3110
                     /locus_tag="KW562_RS26825"
                     /old_locus_tag="KW562_26810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938511.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3138..3317)
                     /locus_tag="KW562_RS26830"
                     /old_locus_tag="KW562_26815"
     CDS             complement(3138..3317)
                     /locus_tag="KW562_RS26830"
                     /old_locus_tag="KW562_26815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3386..3763
                     /locus_tag="KW562_RS26835"
                     /old_locus_tag="KW562_26820"
     CDS             3386..3763
                     /locus_tag="KW562_RS26835"
                     /old_locus_tag="KW562_26820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938513.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="hypothetical protein"
     gene            complement(3825..4124)
                     /locus_tag="KW562_RS26840"
                     /old_locus_tag="KW562_26825"
     CDS             complement(3825..4124)
                     /locus_tag="KW562_RS26840"
                     /old_locus_tag="KW562_26825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_063938515.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4114..4377)
                     /locus_tag="KW562_RS26845"
                     /old_locus_tag="KW562_26830"
     CDS             complement(4114..4377)
                     /locus_tag="KW562_RS26845"
                     /old_locus_tag="KW562_26830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938517.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            4754..5839
                     /locus_tag="KW562_RS26850"
                     /old_locus_tag="KW562_26835"
     CDS             4754..5839
                     /locus_tag="KW562_RS26850"
                     /old_locus_tag="KW562_26835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WP_063938519.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     gene            5836..8025
                     /locus_tag="KW562_RS26855"
                     /old_locus_tag="KW562_26840"
     CDS             5836..8025
                     /locus_tag="KW562_RS26855"
                     /old_locus_tag="KW562_26840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WP_063938521.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     Typing          complement(8231..8811)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.625"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1843..2570
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.885"
                     /coverage="100.0"
                     /evalue="3.84e-144"
ORIGIN
        1 atcggcctgt ttttcggtct ggctgaactc tgggccggac ggatgcgttt tgtggggctg
       61 agtcataagg agcgacggga gtatgccgat cgctataagc gcgataataa ccggactgcg
      121 ccattttcgg tgctggcttc gctggattcg cccgggcgga tggtcagggc acggcggcat
      181 gtctggaact acgcgtactg gctgaccggc gctgccggtc tggcccttct gggggggctg
      241 gttgtcgccg tgtttatcga gagctgggga gaaaccgtcc tgcgtccgga cctctacgcg
      301 aacgcagatt attacctgct gcagccggct aacctgttcg ggattgtctg gtgtgccgga
      361 gggcttgtcg ccggcatcct tctggcgcgg ctgctggcat ttctgctgct tgacggtcat
      421 tttgcggccg ctgacgaggc ggtgaatgcg aaactgaacc aggagagccg gcgcagcagc
      481 cagcggaccg gagaaatgac cgacgtccgc cacctgcatt tcggggagcc ggtaccggtt
      541 aacgcgctgg ccgatttttc cacagagcag gcccggaaac agcaggcggt gtttctgggt
      601 aaggatgagc agggccagcc ggtgctggtc ccgcgtgata cctggcgcaa gaccaatatt
      661 cagatcctcg gcctgcccgg gagcggcaag agcgtgatgg gcaccaacgc gctcattcgc
      721 tgcgtacgcg atttcgggga cgcggtggtg tacttcgacc ccaaagggga cgcgtgggcg
      781 ccgcacgtct tccgggcgca ctgcccggac ttcaccctgc tggatctgcg gccggggaaa
      841 ccggcgcagc tgaatctgtt tcgcgatctc gaccagtacg ccctgaagaa tctgctggtt
      901 gccgggttta acctgagtga gaccggcgac gtggcggacc actaccgcat cagcgagcag
      961 aaagcggcga agctgattgc ggagcagttc ccgcaggggg caaacattca gcaggtgctg
     1021 gcggcggcat acgcgctgcc ggaggccctg aaaaaggacg tgaaggggct gatcaccaag
     1081 ctggagaacg tggccgacct gagcgtcctg cagacggaca gcggcatcga cgtggcgggg
     1141 ataatcaacg gcggcggctg cctgtacgtc atcggctcga tggacgatga ggcggtgatc
     1201 cgggtgcaga agatgctgtt tgcccgctgc gcgcaaatca tcattgcgcg ggatgagttc
     1261 cggaggtggc cgcacgcgag catcatgctc gatgagatta aatacctgct gtcgaagtac
     1321 gtgctgaacg cgctggggac gctgcgcagt cgggactgca acctgctgct cgcgcaccag
     1381 tcgctgggcg acttcgggca gtgcggccag gatctgcccg ctgagtttgt gaaaaccacg
     1441 gtgctggata acacgccgat ccgctggttc taccgggcgg ccagccagga gtccgcgcag
     1501 tgggcggcgg gccagaccgg ggaaatccgg gtggacgtgg agcgccgccg ggccagccgg
     1561 gaggcgggga acgtggagca tatcagcggg gacagcttca ttcagaagga ggcgcggccg
     1621 ctgtttgacg tgaacaccct gcagcacctg cctgacgggt ttgcggtgat gaccgggctg
     1681 ggcgtggccc ggatggcgtt cagcagcccg ctgcgggttg aacgccggga aatcccgctg
     1741 cagcgcttcc cggtgctggc gaaaacggac ccgctggcgg agtaccagcc ggaggccggg
     1801 cggcagcggc cgggtgatga tgattttgcg gggctgtact gatggcactg gagcgataca
     1861 acgtcagcca cgcgaagcgg caggcgcgga acgcggagaa aacccggctg accctgcgct
     1921 ggctgcggga agagctgtgt tccacggcgg agctggttgc gcgacggctg gggattgcgg
     1981 cggtgcagcc ggtttaccgc ttcctggaca gcctggtggc gaaagggctg ctggtccggg
     2041 cgaaataccc ggttgacggc cgtcaggtca gcgtgtgggg actgacgccg cacggggtgg
     2101 cgttctcgtt cgacgaggac gaaccgctga cggacgttat tccgtttcag ccgtcaaggg
     2161 tgtcggcggc gcagctgccg caccggctgg ccgtgcagtc cctgcgtctc gccatggagg
     2221 cccggggggc ctccggctgg cggtacctgc accgtatggc cctgaagggg atgaaggtgc
     2281 cggacgcgct ggcggagctg gacggcagaa cggtggcgtt tgaggtggag cgcaccgtga
     2341 agagccgcag gcgctatcag gaggtggtgt ccgggtatct gttcaatcgc aaagcgaacg
     2401 gcatcgatga aatctggtac atctgcccgg accgggcgac gcaggtcagg gtgcagcggg
     2461 cgatcctgtc ggtggatgaa atcgtgaatc cacagaccgg cgaggcccgc aaaacggcgg
     2521 agctggaccg ggagcggctg tttgcgtgct ttaaatttat gacaacggag tgagggatta
     2581 tggcgctgta catggtattt attgaagacg cggaggaccg ggacggcgag cgctgcatca
     2641 tgccgaccag cgagcatctt gaagatatgg tggtgctcgc ctggcagacc ggggcgtatg
     2701 ttttctgttt tgtcgaccgg gcccagcatc tggcgatgat gaaatccatc gggatgtatg
     2761 acgagcggct caacagcgac gaaaaagcca cgtacttttt caccggtaaa aatccgcaat
     2821 gggaacggga caaacccgca ggaatgaagc tgtgtgatta cctctatcgc aaggtggagc
     2881 ggttcgccat cgagaacggt gtgcaggtgg ttgatgccgg gaagtgctca ccggagcaga
     2941 ttagcgcgat gattgaggtg gtcagcgagc ggcacgggga aagcgcggtg aacgagaagc
     3001 tgaaagcgga gctgatgcgg gaatataaca gccgctattt taacgtgccg gtgcctgaac
     3061 tgccggagga caaacccgcc cgccggtggt ggccgttcgg caaaaaataa acggcagcac
     3121 cggccatgcc ggtgctgtta ttccttcccg agcttatccc gcatcacctg ccagagtgtt
     3181 tccgcatcct cgtgcagccg ctcgcacatg tcggccgggg tatccatatc cagctcgttc
     3241 agtttctcga gtaacagcag cgactggtcc ttaataaatt tagccatatt gagggcagtt
     3301 ttttcctgct tagtcatgtt ttctgtcctt ccgggtgtgg tgtgcttcct gtcgggtata
     3361 ctgtctcaaa accggaggtg agacaatggc gtgggtgacg gtgagacagg cgacggaact
     3421 gacggggaaa gcccggagtt ccctgtatag ggatatggcg aaaggccgcg tatcataccg
     3481 tactgaagct gatggtggtc gggtggtgga taccagcgaa cttatccggg tgtacggtga
     3541 attaagacag attgagactc acgagcggga cgggttgaga ctgtcggatg agactgaaaa
     3601 aatggcctcc gacgcgttga tcgcggagat aaaagcactc agggaggagg tggccggact
     3661 gcggcaggaa atgcaggcca tgagactgct ggaacacaaa ccagagacag cagagacacc
     3721 ggccggaaca tcgcgccgat ggtggcagtg gggaaaacgc tgacgcgctg tgcttgtcca
     3781 accggcgacg gccacgcaat gaattgcgcc cccatcaccg gttatcagcg aacggtatgg
     3841 ggattctggc gctggccaac cacggccaga acccggactt tcctggtctg gtcatcaaca
     3901 tcatagagga catgctgtcc catcagactg atccggcgaa cgtcctcgcc atagcgtgag
     3961 tccacttcat ataggcgcgg gaagtcggcc agtttgtccg ctgcagcgag aactcgatcg
     4021 gtaacgttct gcgcatccca cagggatgaa ttttccgcaa tgtagtcaaa caatgctgtc
     4081 acatcctcga tggctccctg cttccattcc acctcatacg gcatcgcgta gttcctgata
     4141 gcggccatgc agtctctggc ggagttgttc cgtggtcatg cccgggcgcg tgtcagcgcg
     4201 ggcggcggtc actttagcgc gaacctgatc ttcatattct gcctgggcca gttcttcgtt
     4261 agccaggcgc tgcaacagtg cagctttgtc cgggccacct tcaggcagta tgcggggcag
     4321 tctctcggcc agttcccgca ggaaattggc gttttcatag tgtgtactgt tgctcattgt
     4381 ggagcctccc gggctgatac gtcgatatga aggagtttag cagggggcgg tctgaaagaa
     4441 tatgctcgtg tcagtcaggc tgatgaagac gaaagctggt aaaaaagggc ttacgacacc
     4501 cattttacgt caaactgcac gacatgcagt tgcgtgcata gccatgcagc gggttaaaat
     4561 ttatcaggcg cgtttccggc ggttttccgg gtggtttgtt gccggtttta tcccgtagcc
     4621 gccggaaatg ccctcagacc gtctgagcgg tgcgcgtaat gcgacgttat ggtaaatcat
     4681 gtgattaatg gtagtgataa tcattatcac ttttgctata ttcttgtttc ttttattgta
     4741 aggaaatgta agtatggcaa tcacgattaa atccgccgtt aatgtccgta gcagagtatt
     4801 aatatactct ttactactgt ttactggttc agcgtatgcg gctgatatag ctgaatcaac
     4861 attcctgact cgtgatatcg gcaatggtct gtatgaaatg tcttttgatg gcaaaaataa
     4921 tagtctctgg gcagcatcat cgtcttcatt cgacagagat aagggccacg gaattgttta
     4981 tcagttagaa ccatccactc tgaaaacaaa atctgccgtt aaaatcgcac gtcttgggtt
     5041 tgccacagca ctggatgagg aacatgagcg actttatatc ggcaatactc tggacggctc
     5101 agtgacggtc attgatactc taaccggaaa agaactttca gtaattccgt taactgaggg
     5161 acaggatgag gctcatttca gaccgaccag agaaatggtc attgacaaaa aaaatcagcg
     5221 tctttatgtc tccagcgccg cagataaagg ggctctgtgg gttattgaca tggctgtcgg
     5281 gaagaaaatt aaaaccattg atggactggg gcacgctgtt actggaattg cgttaaataa
     5341 aaatggggat aatatttatc tggttaacgg ggatggtgag attattaacc tggatgccag
     5401 tagctatgaa ataaaaaaac ggtttgttgt agagcctgag aaaaaacact ttttccttaa
     5461 tgtaagtctg gatgaaaaaa acggacgtgc atttattacc gacccggata ttcccggggt
     5521 gctggttgtt gatgtaaact cgggaaaaat actacacaga attgatgtta taaattcact
     5581 ggcgatttta tttaatcctt tacggaacga aatctatatc acgcaccgta atgcaaaaca
     5641 ggtcagcatt gtggacagta attcttatta cgtaaaaaca acgataaaaa cccgcctgat
     5701 gccaaacagt ctgtctttat cacatgacgg tgaaaatctg tacgttagtg taaaacaaga
     5761 aaaaaaagag atgagtacta tgaaggatta catactcaaa atcgaattat ctaaaattgc
     5821 tatggagcca gaagaatgaa taaatattta gggctgagca tagtatatat gatgtataca
     5881 tcatctgtta tggctgatga ttctggcgca gttagtaaag ataaaaatat ttcaccagaa
     5941 gaaacattaa ttgttcatgg gaccgctgtt tctgaaagca caacagaagg aacagattca
     6001 tacacaaccc agtcagccaa tacagcatca aagatgaatc tgtcattccg ggatacacct
     6061 cagtcaacta cagtcattac gcgacgaaga atggatgatg tagcagcaaa aaatattatt
     6121 gatgtggtta aaaccgctcc tggattatat gtcagtacag cagaaggttc cggaagaccg
     6181 aattttatgt ccagaggatt taatgctaat gtcatgtatg aaggatttag cctgccgtgg
     6241 aattcataca ttccaagttc acaaactagt tcagctattt ttgaccgggt ggagatagtc
     6301 cgcggggcaa caggtcttgt tcagggtgcc ggagaccctt cagccgcgat aaatatgata
     6361 cacaagcgtc cgacccggca atatcaggcc agcactaaca tcggtgccgg acggtggaat
     6421 gactaccggg gtggggttga tgtatcagga ccactgaatg acagcgcgac cttacgcgga
     6481 agggcagttg tggatggcga aaacagtgag accttcaggg atagtgagaa acgcaggaat
     6541 aacgtcattt acggaattat ggaggcagat ctgactgata ataccctact tacgattggg
     6601 ggatatcagc agtatgaata taacaatcat tggtggtatg acttacctat ttctggtacc
     6661 ggacaccacc ttaatcttcc tcgctcgacg tttacaggca atgactggga gtttgaaaga
     6721 agtaaaagta aaactgcatt tgcaactctt gagcagaagt tcagtaatag ctggaagctg
     6781 aaattttcaa cgctgcaaaa ctggagaaat gctgacctgt tagggacggc aacataccgg
     6841 cataaagata cccgacaaag tatgtatcag caagtatggg gatcactgag ggaatataat
     6901 tccaaaagct atgacctgtc gcttaatggg gactatgctt tatttggtcg tgttcatcaa
     6961 ttcgtggctg gaacaactca gcaaaaatac accgataaag atacaggtta tacatattcc
     7021 ccaatatata ttgagaatgt taatatattt gatcatggtt cacattcttc gaaaaaaccc
     7081 atgaagatgt caggtgatgc atcctacgac gtcactgaac aaaaaagcgc ctacgcagca
     7141 actcattttc tacttagtga taactggaaa atattgctgg gtggccgtct tgactggtat
     7201 gaatatacaa gtaaaggtac atccattaat acaggttata aagttaccag aaatatcacc
     7261 aaatatgcag gttcaacata caatcttgac gatcatcata ccatttatgg gagctatacc
     7321 gatatattca atccacagtc aagcaccgac ctcaatggta aagtgatcaa gcctattgtt
     7381 ggtaagaatt atgagacagg tattaagggt gagtatttta acggcttgct taatgcctct
     7441 ctggcggtat ttcaggtaga ccaggaaaat cgagccaggc tgcttcccga tcagacaggg
     7501 tgtgttacta atggcaatcc tcaatgctat gaggctagcg gtaaagtacg cacaaaaggg
     7561 atagatttcg aacttcaggg catggttacg ccactctggg aaattggggt gggatataca
     7621 tatgcagata cgcgatatat aaaagatgga gatcgtagta atgaaggcaa gcgattcaat
     7681 actgccgtgt cgccacaaaa tttatttaag atttcgagca tgtacacctt tgacggaaaa
     7741 ttaacaaact ggcgagcagg agtaaacacg tactggcaaa gtaaaatata tgctaacata
     7801 acagacacaa gggatggcaa tccggttatt aataaacaaa aggcttattc aacaactgac
     7861 attattattg gctatactcc aatgccacaa ctggatttac agctcaatat aactaatgtc
     7921 tttgataagg tttattatac atccataggt gaatcgattg gatatggcac ggaaacatat
     7981 ggagaaccaa gaaatgttaa aattagtgca acctatagat tctaaccatg cggattgggt
     8041 cttctcctcg gatttgcccc tataaatccg tcttttgtgg ttccggcgta caaccccctg
     8101 tttgccactt cagttgatgg ttaatcaaat catgtagagg atcttcttga gatcctccat
     8161 ggttcgtcgt aatcttttgc cctgtaaacg aaaaaaccac ctggggaggt ggtttgatcg
     8221 aaggttaagt cagttgggga actgcttaac cgtggtaact ggctttcgca gagcacagca
     8281 accaaatctg tccttccagt gtagccggac tttggcgcac acttcaagag caaccgcgtg
     8341 tttagctaaa caaatcctct gcgaactccc agttaccaat ggctgctgcc agtggcgttt
     8401 ttgcgtgtct ttccgggttg gactcaagtg aacagttacc ggataaggcg cagcagtcgg
     8461 gctgaacggg gagttcttgc ttacagccca gcttggagcg aacgacctac accgagccga
     8521 gataccagtg tgtgagctat gagaaagcgc cacacttccc gtaagggaga aaggcggaac
     8581 aggtatccgg taaacggcag ggtcggaaca ggagagcgca agagggagcg acccgccgga
     8641 aacggtgggg atctttaagt cctgtcgggt ttcgcccgta ctgtcagatt catggttgag
     8701 cctcacggct cccacagatg caccgaaaaa gcgtctgttt atgtgaattc tggcaggagg
     8761 gcgaagccta tggaaaaacg ccaccggcgc ggccctgccg ttttgcctca catgttagtc
     8821 ccctgcttat ccacggaatc tgtgggtaac tttgcgagag ttagcagcgc ccgccgcagt
     8881 ctcacggccg gagcgtagcg actgagtgag ggaggaggcg caatatcccc cctcccgcag
     8941 acggccaccg gacgcgcgga tgtgacatat tgcaccgtgt taccgccttt cccttccctg
     9001 ccgataaatc attcagatag gtgaagtctg tcggttttct gctgaatatt ttacgttaac
     9061 ctatctctgt tgcttttttc tccttgcgga gaaatgcagg tcatccccgc tggggcgtgg
     9121 ctgacttcag gaggaaatcc cctgcttacg ctggctggac gccaccgacg cacggtcttt
     9181 ccccctcttt ctggctcagc tcggctggac gccggccacg ccagaaattc acccggcccc
     9241 cgtcaggctc acgcctgacc acccacaaaa acccacggtg ctgaacgcac ctgacgaagc
     9301 agcaaagctg ctcctgattt tgcgggtggt cagccgctcg ctatggcgaa ctctgacggg
     9361 ggccggaaca ccggaaaaga ccgcactggg gtgccgcctt ttcctctcac acgctggggc
     9421 gtgctcgttg ttcctccggg gctgcgcctt ttccgcttgg ggcggaaaga ggctggcctc
     9481 cgtggtgaaa gctgaatgca atcaccacgg acgtgaccgc cgccgccatg cctcggggca
     9541 tgtccgcggc ttgtgcagca ggggctgcac accacgccgg taaacccgtc gtctgaagga
     9601 gagagattgc atgtttaata cggattcgct tgcctggcag tggcatatcc cggagctggc
     9661 ccggcagatt tacccgtggg tcagctggct gtacagggag tattacggtc tggccggggc
     9721 tttcctggtt
//
