LOCUS       NZ_CP079667             2186 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC27_S471MPC plasmid
            pS471M_P12, complete sequence.
ACCESSION   NZ_CP079667
VERSION     NZ_CP079667.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20130620
            Assembly: GCF_019317185.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC27_S471MPC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC27_S471MPC
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 72.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:29:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,866
            CDSs (total)                      :: 5,740
            Genes (coding)                    :: 5,582
            CDSs (with protein)               :: 5,582
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 65 of 158
            Pseudo Genes (incomplete)         :: 89 of 158
            Pseudo Genes (internal stop)      :: 26 of 158
            Pseudo Genes (multiple problems)  :: 19 of 158
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079667.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC27_S471MPC"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pS471M_P12"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2014"
                     /collected_by="Christian medical college"
     gene            92..646
                     /locus_tag="KW560_RS29165"
                     /old_locus_tag="KW560_29140"
     CDS             92..646
                     /locus_tag="KW560_RS29165"
                     /old_locus_tag="KW560_29140"
                     /EC_number="2.3.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003159191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoroquinolone-acetylating aminoglycoside
                     6'-N-acetyltransferase AAC(6')-Ib-cr5"
                     /protein_id="WP_063840321.1"
                     /translation="MTNSNDSVTLRLMTEHDLAMLYEWLNRSHIVEWWGGEEARPTLAD
                     VQEQYLPSVLAQESVTPYIAMLNGEPIGYAQSYVALGSGDGRWEEETDPGVRGIDQLLA
                     NASQLGKGLGTKLVRALVELLFNDPEVTKIQTDPSPSNLRAIRCYEKAGFERQGTVTTP
                     YGPAVYMVQTRQAFERTRSDA"
                     /name="fluoroquinolone-acetylating aminoglycoside
                     6'-N-acetyltransferase AAC(6')-Ib-cr5"
     gene            777..1607
                     /locus_tag="KW560_RS29170"
                     /old_locus_tag="KW560_29145"
     CDS             777..1607
                     /locus_tag="KW560_RS29170"
                     /old_locus_tag="KW560_29145"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="WP_001334766.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCFL
                     LYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGMEI
                     WNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLTEAW
                     LESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGAGFTA
                     NRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL"
                     /name="oxacillin-hydrolyzing class D beta-lactamase OXA-1"
     gene            1745..>2185
                     /locus_tag="KW560_RS29175"
                     /old_locus_tag="KW560_29150"
                     /pseudo=""
     CDS             1745..>2185
                     /locus_tag="KW560_RS29175"
                     /old_locus_tag="KW560_29150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002075262.1"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /name="CatB-related O-acetyltransferase"
     ARG             93..643
                     /name="aac(6')-Ib-cr5"
                     /gene="aac(6')-Ib-cr5"
                     /gene_name="fluoroquinolone-acetylating aminoglycoside
                     6'-N-acetyltransferase AAC(6')-Ib-cr5"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE/QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             778..1604
                     /name="blaOXA-1"
                     /gene="blaOXA-1"
                     /gene_name="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             1746..2185
                     /name="catB3"
                     /gene="catB3"
                     /gene_name="chloramphenicol acetyltransferase (CAT)"
                     /tool="rgi"
                     /drug_class="phenicol antibiotic"
                     /identity="100.0"
                     /coverage="70.0"
     ARG             3..646
                     /name="AAC(6')-Ib-cr6"
                     /gene="AAC(6')-Ib-cr6"
                     /gene_name="AAC(6'); AAC(6')-Ib-cr"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic; aminoglycoside
                     antibiotic"
                     /identity="98.99"
                     /coverage="107.54"
ORIGIN
        1 tttaagcgtg cataataagc cctacacaaa ttgggagtta gacatcatga gcaacgcaaa
       61 aacaaagtta ggcatcacaa agtacagcat cgtgaccaac agcaacgatt ccgtcacact
      121 gcgcctcatg actgagcatg accttgcgat gctctatgag tggctaaatc gatctcatat
      181 cgtcgagtgg tggggcggag aagaagcacg cccgacactt gctgacgtac aggaacagta
      241 cttgccaagc gttttagcgc aagagtccgt cactccatac attgcaatgc tgaatggaga
      301 gccgattggg tatgcccagt cgtacgttgc tcttggaagc ggggacggac ggtgggaaga
      361 agaaaccgat ccaggagtac gcggaataga ccagttactg gcgaatgcat cacaactggg
      421 caaaggcttg ggaaccaagc tggttcgagc tctggttgag ttgctgttca atgatcccga
      481 ggtcaccaag atccaaacgg acccgtcgcc gagcaacttg cgagcgatcc gatgctacga
      541 gaaagcgggg tttgagaggc aaggtaccgt aaccacccca tatggtccag ccgtgtacat
      601 ggttcaaaca cgccaggcat tcgagcgaac acgcagtgat gcctaaccct tccatcgagg
      661 gggacgtcca agggctggcg cccttggccg cccctcatgt caaacgttgg gcgaacccgg
      721 agcctcatta attgttagcc gttaaaatta agccctttac caaaccaata cttattatga
      781 aaaacacaat acatatcaac ttcgctattt ttttaataat tgcaaatatt atctacagca
      841 gcgccagtgc atcaacagat atctctactg ttgcatctcc attatttgaa ggaactgaag
      901 gttgtttttt actttacgat gcatccacaa acgctgaaat tgctcaattc aataaagcaa
      961 agtgtgcaac gcaaatggca ccagattcaa ctttcaagat cgcattatca cttatggcat
     1021 ttgatgcgga aataatagat cagaaaacca tattcaaatg ggataaaacc cccaaaggaa
     1081 tggagatctg gaacagcaat catacaccaa agacgtggat gcaattttct gttgtttggg
     1141 tttcgcaaga aataacccaa aaaattggat taaataaaat caagaattat ctcaaagatt
     1201 ttgattatgg aaatcaagac ttctctggag ataaagaaag aaacaacgga ttaacagaag
     1261 catggctcga aagtagctta aaaatttcac cagaagaaca aattcaattc ctgcgtaaaa
     1321 ttattaatca caatctccca gttaaaaact cagccataga aaacaccata gagaacatgt
     1381 atctacaaga tctggataat agtacaaaac tgtatgggaa aactggtgca ggattcacag
     1441 caaatagaac cttacaaaac ggatggtttg aagggtttat tataagcaaa tcaggacata
     1501 aatatgtttt tgtgtccgca cttacaggaa acttggggtc gaatttaaca tcaagcataa
     1561 aagccaagaa aaatgcgatc accattctaa acacactaaa tttataaaaa atctaatggc
     1621 aaaatcgccc aacccttcaa tcaagtcggg acggccaaaa gcaagctttt ggctcccctc
     1681 gctggcgctc ggcgcccctt atttcaaacg ttagacggca aagtcacaga ccgcgggatc
     1741 tcttatgacc aactactttg atagcccctt caaaggcaag ctgctttctg agcaagtgaa
     1801 gaaccccaat atcaaagttg ggcggtacag ctattactct ggctactatc atgggcactc
     1861 attcgatgac tgcgcacggt atctgtttcc ggaccgtgat gacgttgata agttgatcat
     1921 cggtagtttc tgctctatcg ggagtggggc ttcctttatc atggctggca atcaggggca
     1981 tcggtacgac tgggcatcat ctttcccgtt cttttatatg caggaagaac ctgcattctc
     2041 aagcgcactc gatgccttcc aaaaagcagg taatactgtc attggcaatg acgtttggat
     2101 cggctctgag gcaatggtca tgcccggaat caagatcggg cacggtgcgg tgataggcag
     2161 ccgctcgttg gtgacaaaag atgtgg
//
