LOCUS       NZ_CP079666             2600 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC27_S471MPC plasmid
            pS471M_P11, complete sequence.
ACCESSION   NZ_CP079666
VERSION     NZ_CP079666.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20130620
            Assembly: GCF_019317185.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC27_S471MPC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC27_S471MPC
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 72.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:29:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,866
            CDSs (total)                      :: 5,740
            Genes (coding)                    :: 5,582
            CDSs (with protein)               :: 5,582
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 65 of 158
            Pseudo Genes (incomplete)         :: 89 of 158
            Pseudo Genes (internal stop)      :: 26 of 158
            Pseudo Genes (multiple problems)  :: 19 of 158
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079666.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC27_S471MPC"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pS471M_P11"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2014"
                     /collected_by="Christian medical college"
     gene            complement(join(2536..2600,1..1050))
                     /locus_tag="KW560_RS29150"
                     /old_locus_tag="KW560_29125"
     CDS             complement(join(2536..2600,1..774,774..1050))
                     /locus_tag="KW560_RS29150"
                     /old_locus_tag="KW560_29125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612067.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_219079074.1"
                     /translation="MTRRPRRNHSPAFKAKVALAAIRGEQTLVELSQQFDVHANQIKQW
                     KDQLLEGATGVFGDETKAEPSGPTIDVKTLHAKIGELTLENDFLSGAPRQGGIAGRKEM
                     IDREHKLSVVRQAKLLGFSRGSVYYLPRPVSDGDLALMRRIDELHLDYPFAGSRMLQGL
                     LRGEGLETGRRHVATLMKKMGIKAIYRRPNTSKPAPGHIRKLAVTRPNQVWAMDLTYIP
                     MARGFVYLCAVVDWFSRKVLSWRLSITMEAAFCIEAVEEALARHGRPEIFNTDQGSQFT
                     SIDFTAVLKRSQIAISMDGKGAWRDNVFVERLWRSIKYEEVYLHAYKTVSEARAGIARY
                     LNFYNTRRPHFSIGIVFCSLEEISRCIRGRQ"
                     /name="IS3 family transposase"
     gene            complement(1142..1684)
                     /locus_tag="KW560_RS29155"
                     /old_locus_tag="KW560_29130"
     CDS             complement(1142..1684)
                     /locus_tag="KW560_RS29155"
                     /old_locus_tag="KW560_29130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_002063889.1"
                     /translation="MIIWINGPFGAGKTTLAERLRDRRPKSLIFDPEEIGFVVKETVPI
                     PASGDYQDLPLWRGLTIAAVSEIRRNYSQDIIIPMTLVHPDYLTEILDGLRQIDDQLLH
                     IFLMLNEDLLRHRISNQTMHPDPNRNAEIREWRLANVARCLAARERLPSTTRVLDSGAH
                     TSDELAAMVLDRLDQRT"
                     /name="AAA family ATPase"
     gene            complement(1697..2557)
                     /gene="aac(3)-IIe"
                     /locus_tag="KW560_RS29160"
                     /old_locus_tag="KW560_29135"
     ARG             complement(1701..2557)
                     /name="aac(3)-IIe"
                     /gene="aac(3)-IIe"
                     /gene_name="aminoglycoside N-acetyltransferase AAC(3)-IIe"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 aatgtgggcg tctggtgttg tagaagttca gatatcgggc gatgccagcg cgtgcctcgg
       61 acacagtctt gtaggcatgg aggtagactt cctcgtattt gatcgaacgc cagagccgct
      121 cgacgaagac attgtctcgc cacgcaccct tgccatccat cgagatggca atctgtgacc
      181 tcttcagcac ggcggtgaag tcgatggagg tgaactgcga tccctggtcg gtattgaaga
      241 tttccggcct gccatgacgg gcaagtgcct cctccaccgc ctcgatgcag aaggctgctt
      301 ccatcgtgat cgacaaccgc catgacaaga ccttccggct gaaccagtcc acgacggcgc
      361 acagatagac aaatccccgc gccatgggga tgtaggtcag gtccattgcc cacacctgat
      421 tgggccgggt gactgccagc tttcgtatgt gccctggcgc tggtttcgag gtgttcgggc
      481 ggcggtagat cgccttgatg cccatcttct tcatcagcgt ggcgacgtgt cgccgcccgg
      541 tctccagacc ttctcctctc aagagccctt gcaacatccg acttccggca aaggggtagt
      601 cgagatgcaa ttcgtcaatc cggcgcataa gggccagatc gccgtcagac accggacgag
      661 gcagatagta gacgctgcca cggctgaagc cgagaagctt cgcctggcgc acgacggata
      721 gcttgtgctc gcggtcgatc atttctttcc gcccagcaat cccgccttgc cgagcgcacc
      781 ggataaaaaa tcgttctcca gtgtcagttc gccgattttc gcgtgcagcg ttttgacatc
      841 gatggtcgga cccgacggct ccgctttcgt ttcatcgccg aacacacctg tcgccccctc
      901 aaggagctgg tctttccatt gcttgatctg gttggcgtgc acatcgaact gctgggacaa
      961 ctccaccagc gtctgctcac ctcggatggc ggcaagtgcc accttcgcct tgaaagccgg
     1021 gctatggttc cggcgcggtc gtctcgtcat ggtctctcct gttcccggca tctaagccga
     1081 agtcaggcag aaactccact tatccccgct gtgcagattt cccgagccag ctctaaggcg
     1141 atcaggtgcg ctgatcgagt ctgtcgagca ccatcgctgc aagttcatcg ctggtgtgtg
     1201 caccactatc gagaacacgg gttgtggacg gaagccgttc ccttgcggcc aagcatcggg
     1261 cgacattcgc taatcgccac tctcgaatct ccgcattccg attcgggtca ggatgcatgg
     1321 tctggttcga gatccggtga cgcaataggt cctcgttaag catcagaaag atgtgcagca
     1381 gctgatcgtc gatctgcctt aacccgtcga gtatctcagt cagatagtcc gggtgcacga
     1441 gcgtcattgg gatgatgatg tcctgcgagt aattcctgcg aatctcgctg accgcagcga
     1501 tcgtaagtcc cctccacaag gggagatcct gatagtctcc gctcgctggt atggggaccg
     1561 tttctttcac cacgaacccg atttcctcag ggtcaaagat cagcgatttc ggacgccgat
     1621 cgcgcagccg ctcagcgaga gtcgtctttc cggcgccgaa aggtccgttg atccagatta
     1681 tcattgtcga cggcctctaa ccggaaggct cgcaagagcg ctgggcggct tcgtgtgctg
     1741 gcacgatcgg agtggctccg aagtgcttct caagataggt gacgccgaac gtcacgatgt
     1801 cctgcgcgtc gaacaggtag cactgagcaa agcccacgac accttctcga tggcgaccga
     1861 gcttcacgta agcatttgct atagtttcga ccgcatccgg ctttccttcg atagcaaagc
     1921 aatcgagaat gccgtttgaa tcgtattctg atgccgtttt ccaggcgact tcaccgtttc
     1981 ttccaagcat cggcatctca tacgtcaccc atcgtttgtt ggggatatcc gcaaccgcct
     2041 cggcgtagtg caatgcggta acggagttta gcggcgcacc caacagcagg gccttcccgc
     2101 caaggcggac gaaccgctcg acgggcgacc cttcccccaa ggcgtgaccg agttcgtgag
     2161 gctccgtcag cgtttcagct agcggaccaa ccgcgaccat cgatgcatcg gggtgcgcgc
     2221 tgcgccgcgc gccgggggct tgaaccagaa attgattcag caggccgaac ccacggtaag
     2281 tcccggccgt tgcgggatcg aacggcggcc aggtacggcg ggctttgtca tccaaccgag
     2341 cgccattcag agtctcctcg tagggtgatc ggtcccacga cgcgtatccc atcacagtgc
     2401 cagtcggccc aaccgcggag cgtaacgcgg caacgaccgt ctccgctcct ccttcgaccg
     2461 gaccaatcgc tttaagtgag gcatgcacca tcaacaggtc accggtttgg actcctaatt
     2521 ttcgaattgc ctccgttatt gccttccgcg tatgcatcgc gatatctcct ctaaactgca
     2581 aaacactata cctatcgaga
//
