LOCUS       NZ_CP079665             4092 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC27_S471MPC plasmid
            pS471M_P10, complete sequence.
ACCESSION   NZ_CP079665
VERSION     NZ_CP079665.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20130620
            Assembly: GCF_019317185.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC27_S471MPC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC27_S471MPC
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 72.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:29:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,866
            CDSs (total)                      :: 5,740
            Genes (coding)                    :: 5,582
            CDSs (with protein)               :: 5,582
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 65 of 158
            Pseudo Genes (incomplete)         :: 89 of 158
            Pseudo Genes (internal stop)      :: 26 of 158
            Pseudo Genes (multiple problems)  :: 19 of 158
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079665.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC27_S471MPC"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pS471M_P10"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2014"
                     /collected_by="Christian medical college"
     gene            complement(join(4033..4092,1..309))
                     /locus_tag="KW560_RS29135"
                     /old_locus_tag="KW560_29110"
     CDS             complement(join(4033..4092,1..309))
                     /locus_tag="KW560_RS29135"
                     /old_locus_tag="KW560_29110"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_267460434.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGCAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGI
                     LADLTHNLIRVYHDAITV"
                     /name="recombinase family protein"
     gene            404..3301
                     /locus_tag="KW560_RS29140"
                     /old_locus_tag="KW560_29115"
     CDS             404..3301
                     /locus_tag="KW560_RS29140"
                     /old_locus_tag="KW560_29115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_001553819.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            complement(3372..4016)
                     /locus_tag="KW560_RS29145"
                     /old_locus_tag="KW560_29120"
     CDS             complement(3372..4016)
                     /locus_tag="KW560_RS29145"
                     /old_locus_tag="KW560_29120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB1"
                     /protein_id="WP_014386481.1"
                     /translation="MALALVGEKIDRNRFTGEKIENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNSSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWIGAQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDNYQASLLMERLGIAVIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB1"
     ARG             complement(3376..4016)
                     /name="qnrB1"
                     /gene="qnrB1"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB1"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 tccattcatg gctatcacgg aaaccttcca gttctccagt tcacgtagcg tattgagcag
       61 atcgagcgtc gagcgccccc accgggaaag ctcagtgacc aggattgcat caatttgtct
      121 ggactgggca agcgccagga ctttctttcg ctcggcccgg tcgagtttag ttcctgaacc
      181 tgtttcctta aatattccca ccacgtcgta gccggcacag ccggcgaagg ctcgcagatc
      241 aaattcctgg cgttcacaag actgatccgc tgttgaaacc cggcagtaaa tggcggcacg
      301 atgtcccaat tgaaccctcc tggatttttg tatcggaacg ccctgattta tatgggctgg
      361 ctgttgtcca aaacagacta tacttcaaaa gggacgaatt tgtatgtcac gacgccatat
      421 tttcaccgaa cggcagcgag cagcgctgtt cgatctgccc acggacgaac tgtcgctact
      481 gaagttctac acgctgggcg atgatgacct ggaaaacatt aggcagcgcc gcagaccgga
      541 aaacaggatt ggctttgccc tgcaactttg tgccttacga tatccgggcc gtgcactggc
      601 tcctggtgag atgatcccgc gtgaagtcct ttccttcgtc ggtgctcagc ttggagttcc
      661 ggctgatgcg cttctcactt atgccacacg gcgccaaacc cgtcagcagc acatggacac
      721 gctgcgcgaa atttacggct acaagacctt cacgggccgt ggtgcccgtg atctgcggga
      781 gtggactttc ggccaggccg aagatgccag atcaaacgag gatcttgctc atcgttttat
      841 tgtgcggtgt cgggaaactt ccaccattct gcccgcagta tcgacaatcg agcgcttgtg
      901 cgcggatgct ctggtcgccg ctgagcggcg gattgaaacg cggattgcgg aaaatttaac
      961 agcggatgtt cgcgatcacc tggacaaact tctgagtgaa atgctcgccg gcaatatcag
     1021 tcgtttcatc tggcttcgca acttcgaggt tggtaacaac tcggctgctg ctaaccgttt
     1081 gctcgacagg ctcgaatttc tgcgtaccct gaatatcaat catagtgctt tggccagcat
     1141 acctgcccat cgcattgccc ggctgcgtcg gcagggtgaa cgctacttca ccgacggttt
     1201 gcgtgacatc acttcggacc gccgctgggc gatccttgcc gtctgtgttg tggagtggga
     1261 agcggcgatt gctgatgcca tagtcgaaac ccatgacagg atcgtaggaa aaacctggcg
     1321 ggaagcgaag cgccagcatg acgaaacaat ttccggctct aaagccacac tcacggatac
     1381 gatccgtacc ttcaccgcgc tgggagcttc gttgcttgag gcccgcagtg acggaacccc
     1441 gctggagatg gctgtcgcca gttcggttgc atgggaccgg ctcgctcaac tggtagcgac
     1501 agggactcaa ctcagcaaca cgctagccga tgagcctctt gcatatgtcg ggcagggata
     1561 ccatcgcttt cgtcgttatg cgccccgcat gttgcgctgt ctgaagctcg aagccgcgcc
     1621 ggtcgccgga ccattggtag cagcagcttt gtcgatcgga gagatgaaag gtgttgcatc
     1681 gccagaaagg cgtttcctgc ggcccagctc caaatggaac cgtcatttac gagctcagga
     1741 aaaaggagat acccgtcttt gggaagtggc ggtactcttt cacctccggg atgcttttcg
     1801 ttccggagat gtctggctcg ctcattcgcg ccgctatggt gacctcaagc aggtactggt
     1861 gccgatgatc gcggcgcagg aaaatgcaaa actggccgtg ccttccaacc cacaggattg
     1921 gctggcagac agaaaggcgc gactcacgat cgctcttaag cggctggccc gggctgcccg
     1981 taacggcact attccgcacg gtagcataga agatggaacg ttgcggatcg acaggttgac
     2041 agcagacgtg ccggatggtg ccgaggcact catactggat ctgtatcgcc gaatgccgtc
     2101 cgttcggatt accgacatgc tgcttgaagt tgatgcagcc cttggtttca cagatgcgtt
     2161 tacccatctg agaaccgggg ctccatgtcg cgaccggatc ggtctgctca acgtcctgct
     2221 cgctgaaggg ctcaatctgg gcctgcgtaa gatggcggaa gctacaaaca cgcatgatta
     2281 ctggcagctc tcacgccttg cccgctggca tgttgaaagc gaagccatga accaggcatt
     2341 ggcaattgtg gtggccgcgc agggtaaact gccgatgtca cgcgtctggg ggatgggcac
     2401 gtcagcatcg agcgatggtc agtttttccc gacagcgcgg catggcgaag ccatgaacat
     2461 ggtcaatgcc aaatatggtt ctgttcccgg cctcaaagcg tatactcacg taagcgacca
     2521 gttcgcgcca ttcgcttgtc agtcgatccc ggcgaccgtg agcgaggcac cgtatattct
     2581 cgatggacta ctgatgaacg aggtcggtcg ccatgttcgc gaacagtatg ccgatacagc
     2641 aggattcacc gaccatttgt tcggagccag tagcctgctc ggctacaatc tcgttctgcg
     2701 aatcagggat ctgccatcga agcggttgta cgtatttaat cccgatacga cccccaggga
     2761 gttacgcaag ttggtaggtg gaaaagcccg ggaggatctt atcgttgcga actggcctga
     2821 tattttccgt tgtgccgcga cgatgaccgc tggcaaaatc aggcccagcc aactcctgcg
     2881 caagctcgct tcttacccac gacaaaacaa ccttgcagtt gcgcttcgtg aagttggtcg
     2941 tattgaacgg acccttttca ttattgagtg gatcctggat acggacatgc agcggcgtgc
     3001 tcagatcggt cttaacaagg gagaggccca ccatgcgctc aaaaatgcgc tccgtatcgg
     3061 gaggcagggg gaaattcgcg atcgcacgac agaggggcag cactaccgaa tcgctgggct
     3121 caatttattg actgcggtga tcatttactg gaataccgtc catcttggtc atgccgtcac
     3181 ggagcggcgg aacgaagggt tggatgttcc ccctgaattt cttccccaca tatccccatt
     3241 gggctgggcg cacattctac tgactggcga atatctttgg cccaaggaac cgaaagctta
     3301 gggtgtcatt tcgccctcag ccggaaccga cccctataag ggcgggagcg gtacttaccg
     3361 ctccgtgaat gctaaccaat caccgcgatg ccaagtcgct ccatgagcaa cgatgcctgg
     3421 tagttgtcca acttaacgcc ttgtaaatca acgccccgaa tatctaagtc acccaactcc
     3481 gaattggtca gatcgcaatg tgtgaagttt gctgctcgcc agtcgaaagt cgaaaactcg
     3541 ccgccggaga gatctgaacc actgaacgtc gcgcccagta cctgggcacc tatccaacgg
     3601 ttttcccaca gctcacactt ttccaacacg actttcgaaa aattggcgta gcttagattg
     3661 gtattcgtga tatatgcgct acaaaaccag gtgcgcgtgg tgatcatatt cataaagctt
     3721 gcgccgcgga aatctgcgcc ttgtgcgcgg cagtggcgaa tttcaatgcc cagcgcactg
     3781 gaattgcgaa aatccgccat ggataaatca cagcttttaa aaatggcatc tttcagcatc
     3841 gcacgactaa aattgcaccc tttctggctt tcacgatcat agaactgaca gccgataaat
     3901 tcagtgccgc tcaggtcggc acctgaaaaa tcacagttaa aaaatgtact attttcaatt
     3961 ttctcaccgg tgaaacggtt tctgtcaatt ttttcgccaa cgagtgccag agccatattt
     4021 gtacctgttt ttttatacag taatggcgtc atggtaaacc ctgatgaggt tatgcgtcaa
     4081 atccgccaat at
//
