LOCUS       NZ_CP079664             4991 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC27_S471MPC plasmid
            pS471M_P9, complete sequence.
ACCESSION   NZ_CP079664
VERSION     NZ_CP079664.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20130620
            Assembly: GCF_019317185.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC27_S471MPC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC27_S471MPC
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 72.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:29:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,866
            CDSs (total)                      :: 5,740
            Genes (coding)                    :: 5,582
            CDSs (with protein)               :: 5,582
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 65 of 158
            Pseudo Genes (incomplete)         :: 89 of 158
            Pseudo Genes (internal stop)      :: 26 of 158
            Pseudo Genes (multiple problems)  :: 19 of 158
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079664.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC27_S471MPC"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pS471M_P9"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2014"
                     /collected_by="Christian medical college"
     gene            complement(join(4190..4991,1..257))
                     /locus_tag="KW560_RS29105"
                     /old_locus_tag="KW560_29080"
     CDS             complement(join(4190..4991,1..257))
                     /locus_tag="KW560_RS29105"
                     /old_locus_tag="KW560_29080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508929.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_042109829.1"
                     /translation="MAIYAVWNNKGGVGKSYLTFQLASEYARQNPTKKVLVVDLCPQAN
                     SSSMLLGGMINGENTLNQIHSAIQRKTISGYIEDRIRSPYIPTNTGSSYVINVSTHNSK
                     VPNNLYLVTGDEQLELQASRVVGASFPGPHDAWRTVHLWINDLISDICNSWNNEDSCVF
                     IDCNPSFSIYTELALTAAERLLIPFSADGSSKRAVKAVLSLVYGERRHQNEPQSDFYLN
                     SNRYSMSLPQIYMYVGNRLTQMNNSSASAFKTVVTEIGDEIWKVWQTKPSLFCIHPNGS
                     STPNNKRSFRNMFQYEVNDANTASVVSGALGIPIATLTAGNKTVSGKIIKVNQTQLDKQ
                     VPNIVALAGMIE"
                     /name="ParA family protein"
     gene            complement(587..753)
                     /locus_tag="KW560_RS29695"
                     /pseudo=""
     CDS             complement(587..753)
                     /locus_tag="KW560_RS29695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            1169..1360
                     /locus_tag="KW560_RS29110"
                     /old_locus_tag="KW560_29085"
     CDS             1169..1360
                     /locus_tag="KW560_RS29110"
                     /old_locus_tag="KW560_29085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032489493.1"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1360..1761
                     /locus_tag="KW560_RS29115"
                     /old_locus_tag="KW560_29090"
     CDS             1360..1761
                     /locus_tag="KW560_RS29115"
                     /old_locus_tag="KW560_29090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032489494.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQLKGEHHSGQVSEQRDTASVDQCEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            2556..2960
                     /locus_tag="KW560_RS29120"
                     /old_locus_tag="KW560_29095"
     CDS             2556..2960
                     /locus_tag="KW560_RS29120"
                     /old_locus_tag="KW560_29095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="WP_008321396.1"
                     /translation="MTILVTIQAQLVVGEEQVIKSLAPEGMLAAVFEDDGRTGYFYALD
                     ESVEGNPIMDAVHIYNAEDVSDGHIPSDVKIGWSEDSQKCVLLINGYPHGAFDFVGKNG
                     YCRSGYPPPINKVWSLSGHEWSDSVDDFFR"
                     /name="DUF2251 domain-containing protein"
     gene            complement(3054..3485)
                     /locus_tag="KW560_RS29125"
                     /old_locus_tag="KW560_29100"
     CDS             complement(3054..3485)
                     /locus_tag="KW560_RS29125"
                     /old_locus_tag="KW560_29100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_042109831.1"
                     /translation="MKIPAHLKHKPIIQVENYDRIDGPHADDTDAMGLSVGIAQWNTPG
                     WTELSAKVWRNTGEKWSRQSEELPLHRVIDLATLICITMDYSKNGRLSSSDDFPVTRST
                     DNPELAHHTEFMKKELKQNKSNLDVSLKRLSTELKRLGY"
                     /name="DUF6530 family protein"
     gene            3903..4160
                     /locus_tag="KW560_RS29130"
                     /old_locus_tag="KW560_29105"
     CDS             3903..4160
                     /locus_tag="KW560_RS29130"
                     /old_locus_tag="KW560_29105"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042109830.1"
                     /translation="MGSNNNQNKDLQDKQADLLDQTYTLGWMDGYAEGYDDAIEDTQNA
                     LNKLENENELLTEVVESKDNVIKELASSAGRALQAVKKNK"
                     /name="hypothetical protein"
     Typing          complement(646..1048)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.857"
                     /coverage="99.0"
                     /evalue="1.65e-171"
ORIGIN
        1 atatatggac ttctgatcct atcctcaata tatccagata ttgtctttct ctgtattgct
       61 gaatgaatct ggtttagtgt gttttcgcca tttatcattc caccaagtaa catagaggac
      121 gagttcgctt gtgggcacag gtcaaccacc aggacctttt tagtagggtt ctgtctggca
      181 tattcagagg ctaactgaaa agtaaggtaa cttttcccta caccaccctt gttattccat
      241 acggcataga tcgccatcta taactcctaa tctagttttg ttgttagctt agattcaggg
      301 tactagagat tattgttcta ctgttagttt ctttcagctg actgcctttt tttggaacga
      361 tattcagtcg aaggggttgg ctggctcacc atgacttatc tggagttgtc gaattccgat
      421 taaaaggatc ttcttgagat cctaacctgc gggatgtaat cttttgctct gtaaacgaaa
      481 aaaccacctg gggaggtggt ttttcgaagg atcagtaagt tggggaactt ctgaaccgtg
      541 gtaacagggt gtacaagacc gctgccacca aatctgtcct ttcagtttag ctgtagttag
      601 gctgtaactt caagaactct gcaccagtaa ttcttgtaca ccccttacca gtggctgctg
      661 ccagtggcgt tttgtcgtgc cttccgggtt ggactcaagt cgaaagttac cggaacaggc
      721 gcagcagtcg gactgaacgg ggggttcgtg catacagtcc agcttggagc gaactgccta
      781 cccggaactg agtgtcaggc gtggaatgag acaaacgcgg ccataacagc ggaatgacac
      841 cggtaaaccg aaaggcagga acaggagagc gcacgaggga gccaccaggg ggaaacgcct
      901 ggtatcttta tagtcctgtc gggtttcgcc accactgatt tgagcgtcag atttcgtgat
      961 gcttgtcagg ggggcggagc ctatggaaaa acggccgctg tgcggcctct ccttctcccc
     1021 atcacgcgat cgctcggttt tctcgagctt ttatacgcat gctcctgccg ctcgccgcag
     1081 ccgaacggca ggagcgtagc gactgagtga gcgaggaagc ggaaaagaga acatccctac
     1141 actgagcact gacgctctga ggcctcttat gacgaaacaa cagaaaacag cgctgaacat
     1201 ggctaaattc atccagcatc aatctctgct cctgctggag aagcttaacg agcttgatct
     1261 tgatgtcgag gccgaccttt gtgagaaact ccacgatgat gcggagcatc ttttccgtac
     1321 actgtcctcg agactggatg cattacagga tggtaactga tggccagagt gtctattagt
     1381 gaggcagccc gactgacggg taagagtaga acaacactgc acaggttaat taaagcaggt
     1441 gacctgtcca cctgtagcgg tgagcgcaat gcaaaaatgg tagatacttc agagctttta
     1501 cgtgtctttg gtccctttga acaactgaag ggtgaacacc attctggaca ggtctctgaa
     1561 cagcgcgata cagcctctgt tgaccagtgt gaacaggtga ttcgtcatct gaaacaggag
     1621 attgaacacc tgaacacttt acttttgtcc aaagattcgc acattgacag cctgaaacag
     1681 gccatgctcc ttcttgaaaa tcaccaggca aaattaactg aatccccggc gccatggtgg
     1741 aaattctgga aaaaatcctg accctgtcat gcttcagttt tcctctccgg ccatcccggg
     1801 gagttcctga accttcccaa aaatagtcag atctctgaac gctcctcacc actaaaaata
     1861 cccacacccc acgcaaaatt aaaattttgc agatttgtat ttaatatcag tagtttactt
     1921 tcctgagatt tcgtttttta aattttattt atatagatcc gactctatga cgactcgtca
     1981 tagagtcccc ggcgagtcgt catagagtcg gtaacgactc agggctataa aaacaatgag
     2041 ttaataacga cccgactcta gacccgactc cccgtttggt tgttttttag tcgcggcgac
     2101 tctaggtacg actctagtgc agagtcgccg gtgaatcgtg tggtgagtcg tccggcgact
     2161 tgccataacg tgattgccgg ccgtcgacga acccgattcc tgaacgtgct gaaaaccggt
     2221 tttccggctg cgccggcaac gcttcacggc cacgcaataa attgcgtccc cgttcaccgt
     2281 tttcggatgg gaagaacgcc ggaaacagga cttaccctgt ctgaatggcg tgaatattcg
     2341 cgctatgcac ttgcgtgcat actcatgcat gccataaaaa cagagcctgc gcgtttctgg
     2401 cgggttttcg ggtggtttgt tgcctgtttt accggtttcc cgtcagaaac gccctcagcg
     2461 cgtttctgac gatgctgata acggatgtta tggtaaatta tccgcttgtc tgaaagctgg
     2521 ctgccgaagc aaccaaggaa aggagaacag acctgatgac cattttagtg acgatacagg
     2581 cccagctcgt tgtcggggag gaacaggtta taaagagcct cgctcctgag ggcatgctgg
     2641 ccgccgtgtt cgaagacgat ggccggaccg ggtatttcta tgcgctcgat gagtccgtcg
     2701 agggaaaccc gattatggac gcagtacaca tctacaatgc cgaagacgtt tcagacggtc
     2761 acattccttc tgatgttaaa attggctggt cagaagacag tcagaagtgc gtactcctga
     2821 tcaatggata cccgcacggg gcattcgatt tcgtgggaaa aaacgggtac tgcaggagtg
     2881 gttatcctcc ccctataaac aaggtgtggt ctctttccgg gcatgagtgg agcgactctg
     2941 tggatgattt tttccggtga attacggcag gctatagcct gccattatgg ttcgaaaaac
     3001 ggatgttatg gtaaattcgt ctgtgaagta ggtagacatt tctaaagcta gttttaatag
     3061 ccaagtctct tcaattcagt cgagaggcgt ttgaggctta catcaagatt gcttttattt
     3121 tgttttaact cttttttcat aaactcagtg tgatgtgcta gctcagggtt atcagttgag
     3181 cgagtcacgg gaaaatcatc actggatgaa agtcgcccat ttttactgta gtccatggtg
     3241 atacagatca gagttgcaag gtcgattacc cggtgaagag gcagctcttc tgattgtctt
     3301 gaccattttt ctcctgtgtt tcgccaaacc tttgccgaaa gctcagtcca tccaggagtg
     3361 ttccattgag caatacctac agatagcccc atagcatcag tgtcatcggc atgtgggccg
     3421 tcaatgcggt cgtaattttc gacttgaata attggtttat gcttcaaatg cgcgggtatt
     3481 ttcatcagtt cgtccttagg taatgtttgt gattattaaa ttacattgtt tttctaatta
     3541 taaaactcac taaatgtaag taaatattaa tatctattgt tatcatgtgc ttgtgatttt
     3601 gtttttccaa ttttatgttt tttgcttgga gagtagcggt gtttttttgt gtcaagtgtt
     3661 atgataagcc gtacgttttt tttctggttt atagttcggt tattcacagc aatgggcgtg
     3721 gttttctatg actacagata acgttaggaa tattggcgac tataaaaaga aagatgtcgg
     3781 ggtggatgct gactgtaatc cagtctcagg aaacacacca ccagtcagac taagtgagaa
     3841 acttagaaat ggaattcctc ctggtttgaa aactcagatg cgccaacaaa tgaggaaaaa
     3901 acatgggctc taacaataat cagaataaag atctgcagga taagcaagct gatcttttgg
     3961 accaaacgta tactcttggt tggatggatg gctatgccga agggtatgat gatgctattg
     4021 aagatactca gaacgctttg aataaactgg aaaacgaaaa cgagttgctt actgaggttg
     4081 ttgaaagtaa agataatgta atcaaagagt tggcctctag tgctggcagg gcgctgcaag
     4141 cagtaaagaa aaacaaataa aaaaaggccc ttttaagggc cttgctattt tattcgatca
     4201 taccagcgag agctacaatg ttggggactt gtttatctag ctgcgtctga ttaaccttta
     4261 ttatttttcc tgaaacagtt ttatttcctg cagttaatgt cgcgattgga atacctaagg
     4321 ctccagatac gacagatgct gtgtttgcat catttacttc atactgaaac atattcctga
     4381 aagatctttt attatttggc gttgagctcc catttgggtg aatacaaaac agacttggtt
     4441 ttgtttgcca tactttccaa atttcatcac caatttcggt tactaccgtc ttaaatgcac
     4501 tggcagatga attgttcatt tgcgttaatc tatttccaac atacatatat atttgtggta
     4561 gcgacataga ataacgattg ctattaagat agaaatctga ttgtggttca ttctgatggc
     4621 gacgctcacc ataaactaaa gaaagtacag ccttaactgc tctctttgat gaaccatcgg
     4681 cagagaaagg aattaaaagg cgctctgctg cggttaaagc taactctgta taaatagaga
     4741 agctagggtt gcagtcaatg aatacgcagc tatcttcatt attccatgag ttgcaaatgt
     4801 cactgattaa atcattaatc cagaggtgaa cagtacgcca tgcatcatgg ggacctggaa
     4861 atgatgcccc aacaactctt gatgcttgga gttctaattg ttcatcgcca gtgacaagat
     4921 atagattatt tggaacttta ctattgtgtg tagatacatt tattacatag ctagaaccag
     4981 tgttggtagg a
//
