LOCUS       NZ_CP079663             5129 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC27_S471MPC plasmid
            pS471M_P8, complete sequence.
ACCESSION   NZ_CP079663
VERSION     NZ_CP079663.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20130620
            Assembly: GCF_019317185.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC27_S471MPC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC27_S471MPC
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 72.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:29:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,866
            CDSs (total)                      :: 5,740
            Genes (coding)                    :: 5,582
            CDSs (with protein)               :: 5,582
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 65 of 158
            Pseudo Genes (incomplete)         :: 89 of 158
            Pseudo Genes (internal stop)      :: 26 of 158
            Pseudo Genes (multiple problems)  :: 19 of 158
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079663.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC27_S471MPC"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pS471M_P8"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2014"
                     /collected_by="Christian medical college"
     gene            join(3748..5129,1..>1)
                     /locus_tag="KW560_RS29090"
                     /old_locus_tag="KW560_29065"
                     /pseudo=""
     CDS             join(3748..5129,1..>1)
                     /locus_tag="KW560_RS29090"
                     /old_locus_tag="KW560_29065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016479965.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            complement(77..3094)
                     /locus_tag="KW560_RS29095"
                     /old_locus_tag="KW560_29070"
     CDS             complement(77..3094)
                     /locus_tag="KW560_RS29095"
                     /old_locus_tag="KW560_29070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490160.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element IS3000 family transposase"
                     /protein_id="WP_021740570.1"
                     /translation="MAFEERVQILSEAEQDELYGPPAFTSADQRFFFSLNDKELAIAKS
                     LRHRGQRYMLVVLLGYFKAKPVVLNPGFHQIKQDLKYVYQTVLPGPGCRPFNLTPKENE
                     RIYQRVFQLCNYQRWNVKDHGAALRDYLSQQARAWTAPRHLFDAAIEYCSGQKIAIPAY
                     STLQKIISQVVGDEQEHMAAHLERAMSRGLKQALAELVNGTGPLPFRQLRQSARNFTGT
                     ELEKELIVYRHIQHWMPEVDLLLSTLSLSQKNLQHLAEKVDYYGAKLKRQTVGSQWLYL
                     LCYLQTRWQQALERIADGFVHHVRQTKQKAKDYAQEAVFKDWQKAAKNVSKAAEVLHLF
                     IDDSIDLQLPFATVRQQALSLLTKRDLESVCLFLNEQRRSVDEAMWQYCDEKESLRKGL
                     LRELFLCLRFEGCDGTQHLAAALAKTQNELNGQDAQLQTADTRLLSKKSREFLLDGEGN
                     ILIDRYEWFLYQQIPDRLNGQLTLPDITKYRALDADLIDGEHWRKNKYTLLQQSHFTKL
                     AEEPEKLIKQMAMELDTRLYEVGEYLEQEDNRNIILRNPQGKRFWRLPSASKHHLVNNP
                     FFQQIPTTGIADVLRMVDRDTGFIDCFAHVLGSQSRSRSHEYDLLAILVGNATNQGIYG
                     MAQISDRTYDQLSTIQANYLRLETLNAANDNINNATAKLPIFRYYNIQEDVIHASADGQ
                     KFEARRETFKTRYSSKYFGTQKGVSAMTLIANHAAINARVIGANEHESHYIFDLLMSNT
                     SDIIPDVLSTDTHGVNHVNFALLDLFGYQFAPRYAQVGKVINDMFDVKEDKEHRIQLCL
                     KKPINTHRIAQHWDTIQRIAVSLKQRKTTQATLVRKLSEYKRNHPLLEALTEYNRLVKA
                     NYLLCYIDDASLRNYVQRALNRGEAYHQLRRAVSSVNGDQFRGSSDEEIQLWNECARLV
                     TNAIVYFNSRILSQLLTSFEYQGDTKRIDIVKQASPVAWHNINLKGTYHFELSEKLPDL
                     EELMRSIEGYLPVSEK"
                     /name="Tn3-like element IS3000 family transposase"
     gene            3258..3473
                     /locus_tag="KW560_RS29100"
                     /old_locus_tag="KW560_29075"
     CDS             3258..3473
                     /locus_tag="KW560_RS29100"
                     /old_locus_tag="KW560_29075"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181263236.1"
                     /translation="MSGDANLDQIADVSYLLDTFFAKVTRTALQNAASIAGLMITTEAM
                     VAEAPKKDEPAMPPGGGMGGMGGMDF"
                     /name="hypothetical protein"
ORIGIN
        1 ggggttctag ggattttccg tccaaaaacg acaaagtgct ctggaggccg cgccgtccgt
       61 ggcctccaga ggggtattac ttttcgctga cgggtaaata tccctcgatt gagcgcataa
      121 gctcctccag atctggcaat ttttcgctca attcgaagtg gtaagtcccc ttgaggttaa
      181 tgttgtgcca ggccacaggg gatgcctgtt tgacgatatc tattctcttg gtatctcctt
      241 ggtattcgaa gctggtcaac agctggctga gtatcctgga gttgaagtaa acgatggcat
      301 tggtgaccag gcgagcgcac tcattccata gctggatttc ttcgtctgaa ctgccccgga
      361 actgatcccc attgacgctg ctcacggccc gacgcagttg gtgataggcc tctccccggt
      421 tcagcgcgcg ctgaacatag tttcttaaac tggcatcatc gatgtagcac agtagataat
      481 tcgctttcac caggcgattg tattccgtca gggcttccag cagcgggtga ttgcgcttgt
      541 actccgagag ctttctcacc aaggtggctt gcgttgtttt ccgctgctta agtgatactg
      601 caatccgttg gatggtatcc cagtgctgcg caatacgatg ggtattgatt ggctttttta
      661 agcacagctg aattcggtgt tctttgtctt ccttgacatc aaacatgtca ttgatcactt
      721 tgccaacctg ggcatagcgt ggggcaaact ggtatccgaa cagatccagt aacgcgaagt
      781 tcacatggtt caccccatgg gtatcggttg agagcacatc cggaatgatg tctgacgtat
      841 tgctcatcaa caaatcaaag atgtagtgcg attcatgttc gttggcgccg atcactctgg
      901 cgttgatcgc agcgtgattg gcgatcaagg tcatggcaga aacacctttt tgagtgccaa
      961 agtacttcga cgaataacgg gttttgaagg tctcgcgccg ggcttcgaac ttttgaccat
     1021 cggcactggc gtggatcaca tcttcctgga tgttgtagta ccggaagatg ggtagcttgg
     1081 ctgtcgcgtt attgatgttg tcgttagcag cattcaatgt ttccaggcga agatagttcg
     1141 cctggatagt gctgagctga tcataggtac gatcagagat ctgtgccatg ccgtaaatgc
     1201 cttgattggt tgcattgccg accagaattg ccaacaggtc atattcatgg gaacggcttc
     1261 tggattggga acccagcaca tgagcgaagc agtcaatgaa accggtgtca cgatcaacca
     1321 tgcgcagtac atccgcaatc cccgttgtgg gaatttgctg gaagaaggga ttgttgacca
     1381 gatgatgttt gctggccgaa ggcaggcgcc agaagcgttt accctgcgga ttacgcaaga
     1441 tgatattccg gttgtcttcc tgttcaagat attcgccgac ttcgtacaaa cgggtatcca
     1501 attccatggc catctgtttg atcagctttt caggctcctc cgctaatttt gtaaaatggc
     1561 tctgttgaag cagcgtatat ttgttttttc gccaatgttc cccgtcgatc aggtcggcgt
     1621 cgagtgcccg gtatttagtg atatcaggca gcgtcagctg gccattcagg cgatcaggaa
     1681 tctgttgata gaggaaccac tcataacgat cgatcaggat attcccttca ccatccagca
     1741 ggaattcacg tgattttttg gaaaggagtc tggtgtcggc agtttgcaac tgagcgtcct
     1801 ggccgttgag ttcgttttgt gttttcgcca aggcggccgc taagtgctgg gtgccgtcgc
     1861 agccttcgaa gcgcagacat aggaacaact ctcgcagcaa acctttccgc agactttctt
     1921 tctcgtcgca gtactgccac atggcttcat cgaccgatcg tcgctgctcg ttcaggaaga
     1981 gacatacaga ttccagatcc cttttggtca gcaggctcag tgcttgctgt cttactgttg
     2041 caaacggtag ttgcagatca atgctgtcat caatgaacag gtgcagtact tcagctgcct
     2101 tgctgacatt tttagctgct ttttgccaat ccttaaacac cgcttcctgc gcataatcct
     2161 ttgccttctg tttggtctgt ctgacatgat gcacaaagcc atcggcaata cgttccagcg
     2221 cttgctgcca tcgcgtttga agatagcaca ataaatacag ccattggctg cctacggttt
     2281 gacgtttcag tttggcaccg tagtagtcga ctttctctgc caggtgttgc agatttttct
     2341 gcgacagtga tagcgtgctc aacagcagat ccacttccgg catccaatgc tggatatggc
     2401 gatagacaat cagttctttc tccaactcgg ttccggtgaa gttacgagcc gattgtcgca
     2461 actgtcggaa cggcagtgga cctgtgccgt tcacgagctc cgccagtgct tgcttaagac
     2521 cgcgtgacat tgcacgctca aggtgcgcgg ccatgtgttc ctgttcgtct cccaccacct
     2581 gactgatgat cttttgcagc gtgctgtagg cagggattgc gatcttctgc cccgaacaat
     2641 attcaatggc cgcatcgaag aggtgcctag gcgctgtcca ggctctggct tgctgggata
     2701 agtaatctct caacgccgct ccatggtctt tgacattcca gcgctggtag ttgcagagct
     2761 ggaaaacacg ttggtaaatc cgttcgttct cttttggcgt gagattaaag ggtctacagc
     2821 ctgggcctgg tagaacggtt tgataaacgt atttgaggtc ctgcttgatc tgatgaaagc
     2881 cgggattcag caccaccggc ttggctttga aatagcccag cagtaccacc agcatgtaac
     2941 gctggccccg atgacggagg cttttagcga tcgccagttc cttatcgttc agcgagaaaa
     3001 agaagcgttg gtcggcggag gtgaaagcgg ggggtccata taactcatct tgttctgcct
     3061 cggacaagat ttggacacgt tcttcgaatg ccatggattt agcccaaaaa gactaaatct
     3121 tactcaaaca gagcccaaat agggatctcg aaaaaagtaa tacccctctg gaggccacag
     3181 acggcggggc ctccagagca ctttgtcgtt tttggacgga aaatccctag aaccccaaga
     3241 agggcagcgt cacatccgtg agcggtgatg ccaacctcga ccagatcgcg gacgtgtcgt
     3301 acctgctgga taccttcttt gccaaggtca cccgcaccgc gttgcagaac gccgcctcca
     3361 tcgcgggcct gatgatcacc accgaagcca tggtggccga ggccccgaag aaggacgagc
     3421 cggcgatgcc gccgggcggc ggcatgggtg gcatgggcgg catggatttc tgatgcggtt
     3481 ggcccggtcg tcagggaacc ggaccgcgcc agcgcggtcc gatcccggca acgacccgac
     3541 atcaaggccc caaggacggg gccggagccc ggcagcgatg ccgggctttt tgttgtgccc
     3601 gcgccgcggc aatgtctgac gcgaagatca gaacgcaccg atacgaacgt gcgaacacag
     3661 gtgcaaccct gagcagccgt ccccgcaccg gagcgctgcg tgccgcgcct cgccacatcc
     3721 cggcggcaag ccgcgggatg cgcgccactg ccgtccgccc acaccggttc gcggtacgcg
     3781 cgccacgcgc ccgagcgcac gctgctgtac gcgttggtag aggcgcacta cccggacttc
     3841 attgcacgga tcgaagcgga gggccgctcg ctgcccgggt atgtccgcga ggcgttcgat
     3901 gcctacctgc gttgcggcgt gctcgagcac ggcttcctgc gggtggtgtg cgagcactgc
     3961 cgtgcagaga ggctggtggc cttctcctgc aagaagcgcg ggttctgccc gagttgcggc
     4021 gcgcgacgca tggccgagag tgcgcggcac ctggtcgagg aggtgttcgg cccgcggcct
     4081 gtgcggcaat gggtgctgag ctttccgtac cccttgcgtt tcctgttcgc cagcaagcca
     4141 gaagccattg gcccggtgct gggcatcgtg cagcgcgtga tcgccggctg gttggccgat
     4201 caagccggca tcgaccgcgc cagcgcccag tgcggcgcgg tgacgctgat ccagcgtttc
     4261 ggcagcgcgc tgaacctgaa catccacttc cacatgctgt ggctcgacgg cgtgtacgtg
     4321 gaagccaccg agctgccgcg gcgcgaactg cgcctgcacc gcgcccgtgc gcccaccacc
     4381 gcgcagttga cccagctggc agctgccatc gcgcaccggg tgtgtcggca cctgacgcgc
     4441 aaaggctggc tcgaagggga gggcgaatcg gccttcctgg cagacagcgc tgcaggcgac
     4501 gacagcatgg atgggctgcg gatgagttcg atcacctacc gcatcgccac cggccgcgac
     4561 gctggctgca aggtcgtcac gctgcaaacg ctgcccggtg acgccggttc gctggagggc
     4621 gaagccggca aggtcggcgg cttctcactg catgccggcg tggcggccga agcacacgaa
     4681 agccacaagc tggaaaagct gtgccgctac atcacgcgcc cggcgatcag cgagaagcgg
     4741 ctgtcgatag cgctccaggg cagggtgcgt taccagctca agaccccgtg gcgcaatggc
     4801 accacgcatg tggaatggga tccggtggat ttcatcgcca agctggcggc gctggtcccg
     4861 ccaccgcgcg cccacctgac ccgcttccac ggggtgtttg ccccgaacgc cgccctgcgc
     4921 gcacagctga cgccatcggg gcgcggcagg cggcatgacg ccgctgtgga gccggcggac
     4981 gcaagcgcga acgacgcgcc gcgcagcccc gaggagaagc gccgttcgat gagctgggcg
     5041 caacgcctca agcgggtctt ttccatcgac gtcaccgcct gcgtccactg cggtggcacc
     5101 gtgcggatcg tcgccagcat cgaggaacc
//
