LOCUS       NZ_CP079661             5922 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC27_S471MPC plasmid
            pS471M_P6, complete sequence.
ACCESSION   NZ_CP079661
VERSION     NZ_CP079661.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20130620
            Assembly: GCF_019317185.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC27_S471MPC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5922)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC27_S471MPC
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 72.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:29:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,866
            CDSs (total)                      :: 5,740
            Genes (coding)                    :: 5,582
            CDSs (with protein)               :: 5,582
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 65 of 158
            Pseudo Genes (incomplete)         :: 89 of 158
            Pseudo Genes (internal stop)      :: 26 of 158
            Pseudo Genes (multiple problems)  :: 19 of 158
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079661.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC27_S471MPC"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pS471M_P6"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2014"
                     /collected_by="Christian medical college"
     gene            1..948
                     /locus_tag="KW560_RS29015"
                     /old_locus_tag="KW560_28990"
     CDS             1..948
                     /locus_tag="KW560_RS29015"
                     /old_locus_tag="KW560_28990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_015060053.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     APLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLDLSS
                     YNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQFEQPL
                     PANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSKGGGRPSNSGKDKSDLLPE
                     VLRLKAQGYTNRDIADDLQISPSTVSVYLKRDRP"
                     /name="replication initiation protein"
     gene            1356..1748
                     /locus_tag="KW560_RS29020"
                     /old_locus_tag="KW560_28995"
     CDS             1356..1748
                     /locus_tag="KW560_RS29020"
                     /old_locus_tag="KW560_28995"
                     /inference="COORDINATES: protein motif:HMM:NF037437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_043907050.1"
                     /translation="MSTTQIEKDSRRKAVNGVISSFQLEGEAITDEFKILLEDYINGKI
                     STDEVLAMVSDEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="antitoxin VbhA family protein"
     gene            1730..2068
                     /locus_tag="KW560_RS29025"
                     /old_locus_tag="KW560_29000"
     CDS             1730..2068
                     /locus_tag="KW560_RS29025"
                     /old_locus_tag="KW560_29000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_043907051.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLRELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2049..3503
                     /locus_tag="KW560_RS29465"
     CDS             2049..3503
                     /locus_tag="KW560_RS29465"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_064996073.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGNPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGELKLHDPDDPLNRQAVITPKDL
                     PRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAGFSVVRETKNSLSIADPDGGRN
                     IRLKGVLYEQDFRHGEGLRAELEAAGQRYREASDRRIHEARESYLRGVEIKRGENERRY
                     CRAQPENGLAVAQNVDLDAGQHRPVLERNERDPLVSGGIDSGAASHSPSLGREGRQAKA
                     DPVREEIGAPLRADLRPQSNRLGEGYLLDRAEGVLNDGIGAGIALRVRGFAERIRAAAQ
                     GMAGKLRELGEHVREHQTRGRDQQGSSRAIDEAGERLNAASGRLEQAAGGLDRASQQVA
                     KEREHRLLKALDRGWERGDRSRGFER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4191..4988
                     /locus_tag="KW560_RS29045"
                     /old_locus_tag="KW560_29020"
     CDS             4191..4988
                     /locus_tag="KW560_RS29045"
                     /old_locus_tag="KW560_29020"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-181"
                     /protein_id="WP_015060052.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAA
                     WNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTRIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
                     /name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-181"
     gene            complement(5395..>5547)
                     /locus_tag="KW560_RS29050"
                     /old_locus_tag="KW560_29025"
                     /pseudo=""
     CDS             complement(5395..>5547)
                     /locus_tag="KW560_RS29050"
                     /old_locus_tag="KW560_29025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     ARG             4192..4985
                     /name="blaOXA-181"
                     /gene="blaOXA-181"
                     /gene_name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-181"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          14..935
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.974"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          2050..3500
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.554"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgagaaagt gcgcacagct tgagctgttt cgggagcgcc tgccccggaa accctactat
       61 tccgacgagc ttacaacggg cctacggatt gcggatgttg cccgcgccct tggtgcgcgt
      121 tacatccagc cgaacgggcc aacgcaccgc cactggatcg tttttgacgt agaccatgca
      181 gccgctacgt tatcttggga tgacgtaggc gctccagccc ctaacattac cgtcacgaac
      241 aaggccaacg gccacgcgca tctgatctac ggactggata cgccgatccg cactgccccg
      301 gatggaaatg cagcccctct gcggtatgcc gccgccatcg aggccgcgct ccgggagaaa
      361 ttaggggcgg atatgggcta ctccgggctg atctgcaaga accccctgca tgagcactgg
      421 ctggtgcagg tatgggaacc ccggctctac gatttagcct ggctatccga ttacctcgat
      481 ctgagctcct acaatggccg caaatcgctt cctgagtacg ggcttgggcg aaactgtacc
      541 ctgttcgaga aaacgcgcct gtgggcctat aaagcgatcc ggcagggctg gccggattac
      601 cccgaatggt tggccgcttg tgttgaccgg gcatcaggat ataacgccca gttcgagcag
      661 ccgctccccg caaacgaagt ccggcacacg gccaagtcca ttgccaaatg gacgcaccag
      721 cacctaagcc ccgcagggtt cagagaggag caggcccgga gaggtgcgaa gggcggcaag
      781 gtatccaagg gagggggaag gccgagcaat agcggtaagg acaagtccga tctattgccg
      841 gaggttctgc ggcttaaggc tcaggggtac accaaccgcg atatagcgga cgatcttcaa
      901 atctccccca gtacggtgag cgtttaccta aagcgtgatc gtccgtgatc gaaacaagcc
      961 ttatcagata atagccccct ttaggggggt tcctgcgctc ttgtctttta gccttaaaaa
     1021 atccttttag cgcggatctt tggcgaggtg gacaactgcg cctgtccgtc catcgccccc
     1081 ctgttttttc ggggggtgtg ggcggcagca gttatccacg gagccgctct ttcccttgat
     1141 cttgctctag ggcttgcagt atcccgccca tggttgaata atgtactaag caaggctaca
     1201 cagaggcccc tctacctgct tcgcggtctc gggcctctgt ggccttgttc aggggtgcta
     1261 cgcccccccg aaaccccctg tggcccccgc tcgatccgcc ccttcgggac ggtctgcgcg
     1321 gtggcttctt ggctctggag aatggacgag gccctatgtc aacaacacag atagagaaag
     1381 attcgagaag gaaggcagtc aatggcgtta tttctagttt ccaactagag ggggaggcca
     1441 taaccgatga attcaaaatt ctacttgaag attatatcaa tggaaaaatt tccaccgatg
     1501 aggtgttagc tatggtaagt gacgaaccca caacaacgga atacgactac gaaatcaccc
     1561 cccgcactct cggtcttggc gggggctgga atctgcgttt gctggagaac ggcgaagagg
     1621 tcggcggggg tgttttcccc ttgccggagc actgcgattt tcgggatgaa aaggcgctcc
     1681 agacgctact cgatagcctc tatgaggacg ccttagcgga ggcctctgca tggctggcca
     1741 gccgttaaag cgcctccgct ctatcaagat ccgggtctca gatgcggagc ttgagcgcct
     1801 gcgggagctc tgccccaagg cccaactggc ggagtggatg cgggagcaat gtctcggtgt
     1861 tgcgcaaccc cagcgccgaa cgccggcgcc taccgtagat ccggcgctct tgcggcagtt
     1921 ggccggaatg ggcaacaacc tgaaccagat cgcccggcgc gttaattccg gggagtgggg
     1981 gccgttggat cggctgcgca tcatagcgga gctgtccgct ataggccgcg agctggagga
     2041 actgcaccat gatcatcaaa ttccataacc gtggggtagg ccggggctcc gggcctgttg
     2101 gctacttgtt gggccgtgac gggaaccggg aaggcgcaac gctcgaccgg ggcaaccctg
     2161 cggtgattga agccctgatt gattccagtc cctacgccaa aaaatacacc tctggcgttc
     2221 tgtcgtttgc cgaggctgat ttacccaggg cgaccaagga caagctgatg agcgactttg
     2281 aaaaggcgct cctgcccgga ctggagggca accagtacgc tgttttgtgg gtagagcacc
     2341 gcgacaaggg gcggctggag ctgaattttg tagtcccgaa catcgaattg cagagcggga
     2401 agcggctgca accctacttc cacaaggccg acaatccgcg cctcgatgcg tggcgaaccg
     2461 tggtaaacgg ggagctgaaa ttgcacgacc cggacgatcc gttaaaccgt caggccgtta
     2521 tcacgccaaa ggatttgccg agggggcaca aggaggccgc tcaggccatt acagacgggc
     2581 ttttacgcat ggccggagca ggggagatcc agagccgcga tgatgtgcgc aaagccttgc
     2641 agggtgcggg tttcagcgtg gtacgcgaaa ccaaaaacag cctgagcatt gccgacccgg
     2701 acggggggcg gaacatccga ttaaaggggg tgctgtatga gcaagatttt agacatggcg
     2761 aaggacttcg agcagagctc gaagcagcag gccaacgcta tcgagaagcg tctgaccgaa
     2821 gaattcacga ggcacgagaa agctatctcc ggggcgttga aatcaagcga ggagaaaatg
     2881 agcgccgcta ttgccgagca cagccagaaa atgggctggc tgttgctcaa aacgtggatc
     2941 tggatgctgg tcagcatcgt cctgttcttg agcgcaacga gcgggatcct ctggtatcag
     3001 ggggaattga tagcggagcg gcaagccact ctccaagcct tggcagagaa gggcggcaag
     3061 ctaaagctga cccagtgcgg gaagaaattg gagcaccttt gcgtgcagat ctccgacccc
     3121 aatccaaccg tttgggggaa ggatacctat tggatcgtgc cgaaggggta ctaaatgacg
     3181 ggattggagc gggaattgct ctgcgcgttc gaggctttgc agagcgcata cgagcagcag
     3241 cacaaggcat ggcaggaaag ctacgcgaac ttggcgagca tgtacgagag caccaaacaa
     3301 gaggccgaga tcagcagggc agcagcagag cgattgacga ggcaggtgaa cggcttaacg
     3361 cagcaagtgg acggcttgag caagcagctg gagggcttga ccgggctagt cagcaagttg
     3421 caaaagaaag ggaacacagg ctcctgaagg cactggatcg aggctgggag cgtggagaca
     3481 gatcgcgagg ctttgagcgt tgacggaaca aaacggccgt cagttataaa aaaaggaaag
     3541 tgctatcact ttccttttat cggcgggaat tatcgttgag ttcttctatg ccatttttca
     3601 tagtagtaac tgtaatcttc atctctacca ccagattcaa tccagtcatc atactcaggg
     3661 ctatttttga aatctgaatc agacatgcta actccttgtt attagttggc ttctatggaa
     3721 gctagcgcac cgtgaagtta tgatcaacga tgtagtccac atcattgtcg aaatgtgcgt
     3781 tcagctagtc ttcggtgttt tctgctttat ttctagcggc gattctcgcg tctgcccatg
     3841 cggcagactt tttagcctgt tctcgttgtt cttcggttga agactcgctg tggataactt
     3901 gcagagttta ttaagtatca ttgcagcaaa gatgaaatca atgatttatc aaaaatgatt
     3961 gaaaggtggt tgtaaataat gttacaatgt gtgagaagca gtctaaattc ttcgtgaaat
     4021 agtgattttt gaagctaata aaaaacacac gtggaattta ggtaatagtt aaagcttgtg
     4081 cctgattgtt gccacaattg gtcagcagtt tgctagggaa tgagagggca gagaatgata
     4141 agcttagcac ttaatttgta tggatatatt gcattaagca aggggacgtt atgcgtgtat
     4201 tagccttatc ggctgtgttt ttggtggcat cgattatcgg aatgccagcg gtagcaaagg
     4261 aatggcaaga aaacaaaagt tggaatgctc actttactga acataaatca cagggcgtag
     4321 ttgtgctctg gaatgagaat aagcagcaag gatttaccaa taatcttaaa cgggcgaacc
     4381 aagcattttt acccgcatct acctttaaaa ttcccaatag cttgatcgcc ctcgatttgg
     4441 gcgtggttaa ggatgaacac caagtcttta agtgggatgg acagacgcgt gatatcgccg
     4501 cttggaatcg tgaccatgac ttaattaccg cgatgaagta ctcagttgtg cctgtttatc
     4561 aagaatttgc ccgccaaatt ggtgaggcac gtatgagtaa aatgctgcac gccttcgatt
     4621 atggcaatga ggatatctcg ggcaatgtag acagtttttg gctcgatggt ggtattcgca
     4681 tttcggctac ccagcaaatc gcttttttac gcaagctgta tcacaacaag ctgcacgttt
     4741 ctgagcgtag tcagcgcatc gtgaaacaag ccatgctgac cgaagccaat ggcgactata
     4801 ttattcgggc taaaacggga tactcgacta gaatcgaacc taagattggc tggtgggttg
     4861 gttgggttga acttgatgat aatgtgtggt tttttgcgat gaatatggat atgcccacat
     4921 cggatggttt agggctgcgc caagccatca caaaagaagt gctcaaacag gagaaaatta
     4981 ttccctagaa gtggttagcg cgtatttgtg tgaaatagcc gtcatataag ctgtaaagat
     5041 atatggacaa aatgctgatg ctcgatgcgc ttgtagttaa tggctcatac ttaatcggtc
     5101 aggcctaatc gacttaagct ccgcgcgtta actttcagcc tgagcattag catcacattc
     5161 ctaaacgtaa aaagcctagc caatttgcta ggcttttttg ttatctcaat ggccatggtc
     5221 actcaacatg ggggtgtgaa taaaggagtg gcgatgtcag taaaggtgaa tgccgctgcg
     5281 ataaagtgcc tagcccatca aggattgtag gctttcttgg ctgctaaagc gtttgacgaa
     5341 aaagtccaag aaggcgctga tattggttct ccatcatttt catctaccgg tttactatag
     5401 gtcaaccagg ccgtccttgc cggcgcttgg gacgcagacg atggcatcaa atgcctcgct
     5461 caaattcgtt tctacagagg cgctttggga ccgcattcgc tttgctttca tgggggcatc
     5521 tgtcaatgtc agacatgaat cctccgatat aggttgattt ttgatctctt caacacttgg
     5581 tgttgtccga ctagagagac ttatatggat cagtattctt gcactgacaa tggtgccact
     5641 caatcccaag gatcgaccta gttagaggcg tagatcaagc aattcaaaac agcgcagaag
     5701 cagaaggagg tggaaatatg ttccagggtg aaaggttaca tgcaactaac gttgctctct
     5761 aggatgtgcc tgctgtaacg tattgtccag aaaagacaaa cccccgttat cgcttgcatc
     5821 ttggcggagc tgcgaaaaac gggggccgtg acgaatcaca agagaagtct atcacatgga
     5881 tcatgccaaa caaccccacg ccgccaataa gcgcggtggt gc
//
