LOCUS       NZ_CP079659             8514 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC27_S471MPC plasmid
            pS471M_P4, complete sequence.
ACCESSION   NZ_CP079659
VERSION     NZ_CP079659.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20130620
            Assembly: GCF_019317185.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC27_S471MPC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8514)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC27_S471MPC
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 72.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:29:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,866
            CDSs (total)                      :: 5,740
            Genes (coding)                    :: 5,582
            CDSs (with protein)               :: 5,582
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 65 of 158
            Pseudo Genes (incomplete)         :: 89 of 158
            Pseudo Genes (internal stop)      :: 26 of 158
            Pseudo Genes (multiple problems)  :: 19 of 158
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079659.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC27_S471MPC"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pS471M_P4"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2014"
                     /collected_by="Christian medical college"
     gene            join(6479..8514,1..970)
                     /locus_tag="KW560_RS28930"
                     /old_locus_tag="KW560_28905"
     CDS             join(6479..8514,1..970)
                     /locus_tag="KW560_RS28930"
                     /old_locus_tag="KW560_28905"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WP_001143760.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGTLGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            1827..2051
                     /locus_tag="KW560_RS28935"
                     /old_locus_tag="KW560_28910"
     CDS             1827..2051
                     /locus_tag="KW560_RS28935"
                     /old_locus_tag="KW560_28910"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071653266.1"
                     /translation="MSNKDSLERKVTPRKNEPVFPMALRVGIAEDICFLELVDFPEKES
                     GRVFYSIALTKKHAKNLIDGLNKFVESGD"
                     /name="hypothetical protein"
     gene            2053..2697
                     /locus_tag="KW560_RS28940"
                     /old_locus_tag="KW560_28915"
     CDS             2053..2697
                     /locus_tag="KW560_RS28940"
                     /old_locus_tag="KW560_28915"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044816498.1"
                     /translation="MKYINHCDSEDYYITNDSIDLGVRYDVSDYNKLELNDLRDPDLFK
                     VLGRAFVVVENSVWESWNDDVKTLDCSGDGYESILKHYESLELSGEFLLFFKKIRSAIS
                     VYNREYEFCTGDLEPSLQAIVQLIRYAPELAAKKDAQIYFDKKSECFGLIYKQTKKSKG
                     TLNLIVKTNNEIIFSFVRKVSGVVKVTGRAYIGDSLDNSIAIKSLFKMMEW"
                     /name="hypothetical protein"
     gene            2697..3173
                     /locus_tag="KW560_RS28945"
                     /old_locus_tag="KW560_28920"
     CDS             2697..3173
                     /locus_tag="KW560_RS28945"
                     /old_locus_tag="KW560_28920"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_077861907.1"
                     /translation="MTVLSTLNNLKSIPCINAFTGRANNSYLWRYVMEDHKDEFGATTI
                     KCFYLREEISEDYVSIYHSNKDYYEGMVRDVHKRIPVGKTKPAIYLSFDAAEVIEQVNK
                     PDKCIDIKDEGYPHLGLWYLFDINDEFTLIEVTTTILELSSFFQRNEGGVVVPL"
                     /name="hypothetical protein"
     gene            complement(3380..3493)
                     /locus_tag="KW560_RS29460"
     CDS             complement(3380..3493)
                     /locus_tag="KW560_RS29460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071653267.1"
                     /translation="MHEPPVQSDCCAFSGNYQLESNPERHDEAPLAAATGN"
                     /name="replication initiation protein"
     gene            3909..4100
                     /locus_tag="KW560_RS28950"
                     /old_locus_tag="KW560_28925"
     CDS             3909..4100
                     /locus_tag="KW560_RS28950"
                     /old_locus_tag="KW560_28925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_044816495.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSGLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4100..4522
                     /locus_tag="KW560_RS28955"
                     /old_locus_tag="KW560_28930"
     CDS             4100..4522
                     /locus_tag="KW560_RS28955"
                     /old_locus_tag="KW560_28930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_044816494.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKNTKVVD
                     TSELIRVFGSLYGGGDTVKHDVKGDAGATGVNISGLHDLQHKIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKLEPSPQVHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4715..5575)
                     /locus_tag="KW560_RS28960"
                     /old_locus_tag="KW560_28935"
     CDS             complement(4715..5575)
                     /locus_tag="KW560_RS28960"
                     /old_locus_tag="KW560_28935"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     gene            complement(5758..6315)
                     /locus_tag="KW560_RS28965"
                     /old_locus_tag="KW560_28940"
     CDS             complement(5758..6315)
                     /locus_tag="KW560_RS28965"
                     /old_locus_tag="KW560_28940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001235713.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     ARG             complement(4719..5575)
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(3381..3788)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.39"
                     /coverage="99.0"
                     /evalue="1.26e-177"
ORIGIN
        1 atggggtgga ggagaagtgg catctgcaga tggaatgcgc tttgttacgc cagtcagaac
       61 aatcaatgcc ggaccgaacc gcaaatactt tggtaataac agagggatca cctggtacaa
      121 ctttgtgtcc gatcagtatt ccggctttca tggcatcgtt ataccgggga cgctgaggga
      181 ctctatcttt gtgctggaag gtcttctgga acaggagacc gggctgaatc caaccgaaat
      241 tatgaccgat acagcaggtg ccagcgaact tgtctttggc cttttctggc tgctgggata
      301 ccagttttct ccacgcctgg ctgatgccgg tgcttcggtt ttctggcgaa tggaccatga
      361 tgccgactat ggcgtgctga atgatattgc cagagggcaa tcagatcccc gaaaaatagt
      421 ccttcagtgg gacgaaatga tccggaccgc tggctccctg aagctgggca aagtacaggt
      481 ttcagtgctg gtccgttcat tgctgaaaag tgaacgtcct tccggactga ctcaggcaat
      541 cattgaagtg gggcgcatca acaaaacgct gtatctgctt aattatattg atgatgaaga
      601 ttaccgccgg cgcattctga cccagcttaa tcggggagaa agtcgccatg ccgttgccag
      661 agccatctgt cacggtcaaa aaggtgagat aagaaaacga tataccgacg gtcaggaaga
      721 tcaactgggc acactggggc tggtcactaa cgccgtcgtg ttatggaaca ctatttatat
      781 gcaggcagcc ctggatcatc tccgggcgca gggtgaaaca ctgaatgatg aagatatcgc
      841 acgcctctcc ccgctttgcc acggacatat caatatgctc ggccattatt ccttcacgct
      901 ggcagaactg gtgaccaaag gacatctgag accattaaaa gaggcgtcag aggcagaaaa
      961 cgttgcttaa cgtgagtttt cgttccactg agcgtcagac cccagttcct gaaccttccc
     1021 aaaaatcgcc agatctctga acgctcctca ccactaaaaa tatccacacc ccacgcaaaa
     1081 ttaaaatttt gcagatttgt attttatatc agtagtttac gttctataaa tttcgttttt
     1141 ttaattttat ttatacagat ccgactctat gacgactcgt catagagtcc ccggcgagtc
     1201 gtcatagagt cggtaacgac tcaggactat aaaaacaata agttaacaac gacccgactc
     1261 tagacccgac tccccgtttg gttgtttttt agacgtgacg actctgggta cgactctagt
     1321 gaagagtcgc cggtgaatcg tgtggtgagt cgtccggcga cttgccataa cgtgattgcc
     1381 ggccgtcgac gaacccgatt cctgaacgtg ctgaaaaccg gttttccggc tgcgccggca
     1441 acgcttcacg gccacgcaat aaattgcgtc cccgttcacc gttttcggat gggaagaacg
     1501 ccggaaacag gacttaccct ggctgaatgg cgtgaatttt cgcgctatgc acttgcgtgc
     1561 atactcatgc atgccgtaaa aacagagcct gcgcgtttct ggcgggtttt cgggtggttt
     1621 gttgcctatt ttaccggttt cccgtcagaa acgccctgag gccgttttag cggtgcgtac
     1681 aattttggtg ttatgttaaa tcatggccac aaaattccat aaagtgacca atgtaatgtt
     1741 aaatgagcat tgtggatgaa agatgttgtt ttatggtatt attttttatg ttctaaactt
     1801 aaatttattt ctatcggggt attataatga gcaataaaga ttctttagaa agaaaagtaa
     1861 caccaagaaa aaatgaacct gtattcccga tggctctaag agttgggata gctgaagata
     1921 tttgtttttt agagttagtg gactttcctg aaaaagaatc aggaagggtt ttttattcca
     1981 tagctctaac caaaaaacat gcaaagaacc ttatcgatgg attgaataaa ttcgttgagt
     2041 caggtgatta gtatgaaata cattaaccac tgcgatagtg aagattatta tattactaat
     2101 gatagcattg atctcggtgt tcgttatgat gtttctgatt ataataagtt agagctaaat
     2161 gatttaagag atccggattt gtttaaagtt ttgggtcgag catttgttgt tgttgagaat
     2221 tctgtttggg agtcttggaa tgatgatgtt aaaacattag attgctctgg tgacggttat
     2281 gagtcaattt taaaacatta cgaatcatta gaattatcag gtgaattctt attgtttttt
     2341 aaaaaaataa gaagtgccat ttctgtttat aatagagaat acgagttctg cactggtgac
     2401 ctagaaccat ctttgcaggc tattgttcag ttgattaggt atgcccctga acttgctgca
     2461 aaaaaagacg ctcagattta ctttgataaa aaatcagagt gttttgggct tatatataaa
     2521 caaactaaga aaagcaaagg cacgctaaat ttaatagtga aaacaaataa cgagattatt
     2581 ttttcctttg ttagaaaagt ttctggagtg gttaaggtta caggtagggc atatattggc
     2641 gatagccttg ataactccat tgctatcaaa tcattattta aaatgatgga gtggtgatga
     2701 ctgtgttatc aactttaaat aacctcaaat caatcccttg tattaatgca tttacgggca
     2761 gagcaaataa ttcatattta tggcgttatg ttatggaaga tcataaagat gagtttggcg
     2821 ctacaactat taagtgtttt tatcttaggg aggaaatttc agaggattat gtgtctattt
     2881 atcattcaaa taaagattat tatgaaggaa tggtaaggga tgtacataaa agaatccctg
     2941 tagggaaaac caaacctgcc atatatctct catttgatgc agcggaagtg atcgagcaag
     3001 ttaacaaacc agataaatgt attgatataa aggacgaagg ttaccctcac ttgggtttgt
     3061 ggtatttatt tgatattaat gatgaattta ctttgattga ggtgactaca actattttgg
     3121 aattatcctc tttttttcaa agaaatgaag gtggcgtggt tgttcctctt tagcgaaaac
     3181 cgtcttgacg tcatcaagtg tgacctagta ctcttcttga aaaagaaaaa ccgcctttgc
     3241 ggggcggttt ttcttaggtt ctaagagttt gcggctctat gaaccaaggt aactggctag
     3301 ttagaggagc gcagtaacca aaatcgttct ttcagtttag ccttaactgg cacataactt
     3361 caagactaac tcctctaaat cagttaccag tggctgctgc cagtggcgct tcgtcgtgtc
     3421 tttccgggtt ggactcaagt tgatagttac cggaaaaggc gcagcagtcg gactgaacgg
     3481 ggggttcgtg catacagtcc agcttggagc gaactgccta cccggaactg agtgtcaggc
     3541 gtggaatgag ataaacgcgg ccataacagc ggaatgacac cggtaaaccg aaaggcagga
     3601 acaggagagc gcacgaggga gccaccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3661 gggtttcgcc accactgatt tgagcgtcag atttcgtgat gcttgtcagg ggggcggagc
     3721 ctatggaaaa acggccgctg tgcggcctct ccttctcccc atcacgcgat cgctcggttt
     3781 tctcgagctt ttatacgcat gctcctgccg ctcgccgcag ccgaacggca ggagcgtagc
     3841 gactgagtga gcgaggaagc ggaaaagaga acatccctac actgagcact gacgctctga
     3901 ggcctcttat gacgaaacaa cagaaaacag cgctgaacat ggctaaattc atccagaatc
     3961 agtctctgct cctgctggag aagcttaacg agcttgatct tgatgtagag gccgaccttt
     4021 gtgagaaact ccacgatgat gcggagcatc ttttccgtac actgtcttcg ggactggatg
     4081 aattacagga tggaaactga tggccagagt gtctattagt gaagcggctc ggcttgttaa
     4141 ggttagtcgc cccacaattt acaagatgat taacagtggg aaactaagtt acacctctgt
     4201 tgtaaagcat gggaaaaata ctaaagttgt tgatacatca gagcttatta gggtttttgg
     4261 ctcgctttat ggtgggggcg acactgtaaa acatgatgta aaaggtgacg caggcgctac
     4321 aggggttaac atctctggtt tacatgattt acagcataaa attgctttgc ttgaagctga
     4381 aaacgatggt cttaaagggg ctgttaaagc tcgtgatgaa catatcgatt ctcttcgcca
     4441 ggctatgcag cttctggaac acaagctcga accctctcca caggttcatt ctccgtggtg
     4501 gaaattctgg aagaaatcct gaccccgtca tgcttcagtt ttcctctccg gccattccgg
     4561 ggagttcggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     4621 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     4681 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     4741 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     4801 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     4861 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     4921 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     4981 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg
     5041 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     5101 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     5161 ttgtcagaag taagttggca gcagtgttat cactcatggt tatggcagca ctgcataatt
     5221 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     5281 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
     5341 ataccgcacc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     5401 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5461 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5521 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacgaaa atgttgaata ctcatactct
     5581 tcctttttca atattattga agcatttacc agggttattg tctcatgagc ggatacatat
     5641 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     5701 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     5761 cgaggccctt tcgtcttcaa gaattttata aaccgtggag cgggcaatac tgagctgatg
     5821 agcaatttcc gttgcaccag tgcccttctg atgaagcgtc agcacgacgt tcctgtccac
     5881 ggtacgcctg cggccaaatt tgattccttt cagctttgct tcctgtcggc cctcattcgt
     5941 gcgttctagg atcctccggc gttcagcctg tgccacagcc gacaggatgg tgaccaccat
     6001 ttgccccata tcaccgtcgg tactgatccc gtcatcaatg aaccggactg ccacgccctg
     6061 agcgtcaaat tcctttatca gttggatcat atcggcagtg tcgcggccaa gacggtcgag
     6121 cttcttaacc agaatgacat caccttcctc caccttcatc ctcagcaaat ccagcccttc
     6181 ccggtctgtt gaactgccgg atgccttatc ggtaaatata cggtttgctt tcacacctgc
     6241 gtctttgagt gctctgacct gaagatcaag agactgctga ctggttgaga cccgagcgta
     6301 accaaaaagt cgcataaaaa tgtaccttaa atcgaatatc ggacaactca tgtctattat
     6361 tacaaattta cgatttaata gacatattaa tgtaacagtt ttacgatgtc cgataattta
     6421 taacatttcg tacggttgga aaaatgttac taaatgcccg tcaggcaggg aggccgatat
     6481 gcccgttgac tttctgacca ctgagcagac tgaaagctat ggcagattca ccggtgaacc
     6541 ggatgagctt cagctggcac gatattttca ccttgatgaa gcagacaagg aatttatcgg
     6601 aaaaagcaga ggtgatcaca accgtctggg cattgccctg caaattggat gtgtccgttt
     6661 tctgggcacc ttcctcaccg atatgaatca tattccttcc ggcgtccggc attttaccgc
     6721 cagacagctc gggattcgtg atatcaccgt tcttgcagaa tacggtcaga gggaaaatac
     6781 ccgccgtgag catgcagcgc tgatacgtca gcactatcag tatcgtgaat ttgcctggcc
     6841 ctggacattt cgccttaccc gtcttttata tacccggagc tggataagca acgaacgtcc
     6901 tggcctgctt ttcgatctgg cgacagggtg gcttatgcaa catcgtatta ttctccccgg
     6961 agccactacg ctgacccggt tgatttcaga ggtaagggaa aaggcgacgt tgcgcctgtg
     7021 gaacaaactg gcactgatac cgtcagccga acagcgttca cagctggaga tgctgctggg
     7081 gccaactgat tgcagccgcc tgtctttact ggaatcactg aaaaagggcc ctgtgaccat
     7141 cagtggtccg gcgtttaatg aagcaattga acgctggaaa actctgaacg attttggcct
     7201 gcatgctgaa aacctgagta cactcccggc tgtgcgcctg aaaaatctcg cacgttatgc
     7261 tggtatgact tcggtgttca atattgccag gatgtcaccg cagaaaagga tggcggttct
     7321 ggttgccttt gtccttgcat gggaaacgct ggcgctggat gatgcattgg acgttctgga
     7381 cgccatgctg gccgttatca tccgtgacgc cagaaagatt gggcagaaaa aacggctccg
     7441 ctcgctgaag gatctggata aatctgcatt ggcgctcgcc agcgcatgtt cgtacctgct
     7501 gaaagaagaa acaccggacg aatcgattcg tgctgaggtg ttcagctaca tcccaaggca
     7561 aaagctggct gaaatcatca cgcttgtccg tgaaattgcc cggccctcag acgataattt
     7621 tcatgaagaa atggtggagc agtacgggcg cgttcgtcgt ttcctgcccc atctgctgaa
     7681 taccgttaaa ttttcatccg cacctgccgg ggttaccact ctgaatgcct gtgactacct
     7741 cagccgggag ttcagctcac ggcggcagtt ttttgacgac gcaccaacgg aaattatcag
     7801 tcggtcatgg aaacggctgg tgattaacaa ggaaaaacat atcacccgca ggggatacac
     7861 gctctgcttt ctcagtaaac tgcaggatag tctgaggcgg agggatgtct acgttaccgg
     7921 cagtaaccgg tggggagatc ctcgtgcaag attactacag ggtgctgact ggcaggcaaa
     7981 ccggattaag gtttatcgtt ctttggggca cccgacagac ccgcaggaag caataaaatc
     8041 tctgggtcat cagcttgata gtcgttacag acaggttgct gcacgtcttt gcgaaaatga
     8101 ggctgtcgaa ctcgatgttt ctggcccgaa gccccggttg acaatttctc ccctcgccag
     8161 tcttgatgag ccggacagtc tgaaacgact gagcaaaatg atcagtgatc tactccctcc
     8221 ggtggattta acggagttgc tgctcgaaat taacgcccat accggatttg ctgatgagtt
     8281 tttccatgct agtgaagcca gtgccagagt tgatgatctg cccgtcagca tcagcgccgt
     8341 gctgatggct gaagcctgca atatcggtct ggaaccactg atcagatcaa atgttcctgc
     8401 actgacccga caccggctga actggacaaa agcgaactat ctgcgggctg aaactatcac
     8461 cagcgctaat gccagactgg ttgattttca ggcaacgctg ccactggcac agat
//
