LOCUS       NZ_CP079648             5691 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC24_S468MC plasmid
            pS468M_P3, complete sequence.
ACCESSION   NZ_CP079648
VERSION     NZ_CP079648.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20130528
            Assembly: GCF_019317165.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC24_S468MC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5691)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC24_S468MC
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 65.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:15:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,644
            CDSs (total)                      :: 5,518
            Genes (coding)                    :: 5,380
            CDSs (with protein)               :: 5,380
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 60 of 138
            Pseudo Genes (incomplete)         :: 73 of 138
            Pseudo Genes (internal stop)      :: 24 of 138
            Pseudo Genes (multiple problems)  :: 17 of 138
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079648.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC24_S468MC"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pS468M_P3"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2014"
                     /collected_by="Christian medical college"
     gene            28..141
                     /locus_tag="KW559_RS28550"
                     /old_locus_tag="KW559_27915"
     CDS             28..141
                     /locus_tag="KW559_RS28550"
                     /old_locus_tag="KW559_27915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071789001.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(510..1630)
                     /locus_tag="KW559_RS27945"
                     /old_locus_tag="KW559_27920"
                     /pseudo=""
     CDS             complement(510..1630)
                     /locus_tag="KW559_RS27945"
                     /old_locus_tag="KW559_27920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611476.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            complement(1819..2811)
                     /locus_tag="KW559_RS27950"
                     /old_locus_tag="KW559_27925"
     CDS             complement(1819..2811)
                     /locus_tag="KW559_RS27950"
                     /old_locus_tag="KW559_27925"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="WP_087874902.1"
                     /translation="MNKSIESLQVIRAIAACSVVLTHIYGASVPVIGSSGIFGVDLFFV
                     LSGYLMTITYRKNRTGYQFIKSRILRIYPPYLVLSLPLIIFSYFYKNISASDIFHNITL
                     LPTIHGNYDRINFVCWTLTYELYFYIVFSLCMYIFKGARNVILSTSFTMLVLFFLAGGV
                     NGGLAGWKDNSIHHMIGNYLILDFILGSLIGLAYNGYTYKKNAFPILLSIAIIITSCTI
                     IGHIHLPEPYNNYQLITFLTSGIPACVVIYITSKVSVDKIPLKKALIYIGTASYSIYLT
                     HLYAVFAFSKLTHNNAPEKFLCFVTSIVIGCVAYYTIERKIIIKKKNHV"
                     /name="acyltransferase"
     gene            complement(2898..3595)
                     /locus_tag="KW559_RS27955"
                     /old_locus_tag="KW559_27930"
     CDS             complement(join(2898..3346,3346..3595))
                     /locus_tag="KW559_RS27955"
                     /old_locus_tag="KW559_27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558270.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1X4 family transposase"
                     /protein_id="WP_223335786.1"
                     /translation="MASISISCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTILRHFKKLRPQSVNSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGELHVISKRYTQRIERHNLNLRQYLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1X4 family transposase"
     gene            3879..3989
                     /locus_tag="KW559_RS27960"
                     /old_locus_tag="KW559_27935"
     CDS             3879..3989
                     /locus_tag="KW559_RS27960"
                     /old_locus_tag="KW559_27935"
                     /inference="COORDINATES: protein motif:HMM:NF033853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein"
                     /protein_id="WP_139102090.1"
                     /translation="MDWISLAIAIALLVVAVYSGISYFREKKTISRVFRR"
                     /name="small membrane protein"
     gene            5043..5264
                     /locus_tag="KW559_RS27965"
                     /old_locus_tag="KW559_27940"
     CDS             5043..5264
                     /locus_tag="KW559_RS27965"
                     /old_locus_tag="KW559_27940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016162091.1"
                     /translation="MEFDTAAELAALQAQTRRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLRARRIKVVLSTVTRWLEKNG"
                     /name="hypothetical protein"
     Typing          29..141
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.491"
                     /coverage="100.0"
                     /evalue="5.74e-46"
ORIGIN
        1 aaggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgactgctgc
       61 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacatgcaaa agcgccactg
      121 gcagcagcca ctggtaactg atttagagga gatagtcttg aagttatgcg ccggttaagg
      181 ctaaactaaa cgaacagttt tggtgactgt gctcctccaa gccagttacc tcggttcaaa
      241 gagttggtag ctcagagaac cttcgaaaaa accgccctgc aaggcggttt tttcgtttac
      301 tgagcaagag attacgatgc taaagaaagg atctcaagag gatcctgttc catatgccta
      361 cccctgaagt gaaaccagca ccactccgcg acaccaacca tcaaaataaa actatttaac
      421 tcgttgtttt tgataagaaa aaaacatata taatcatgat ccataatcat ccaacgcggt
      481 tgacctgccc ccaggattag atacaacctt cagttagtaa tgtcggttcg tttttcttca
      541 tatttcccgt ttcgccagcc cgccgcaaat tcagccggag tctggtagtt cagcgatgaa
      601 tgtggacgac actcgctcta atcctgcggc cagtcattaa tcgttttcct cgcgtgaacg
      661 atatcgcgga accagtgctc attgaggcac tcatccctga atcgtccgtt aaaactttca
      721 ataaatccgt tctgtgttgg cctgcccggc tggataagcc gcagctccac cccatgctca
      781 taagcccact ggtcgagtgc tctgcaggta aactccgccc cctggtcagt tcttatcgtc
      841 gccggatagc cgcgaaacag tgcaatgctg tccagcattc gcgtgacctg aacgcctgaa
      901 atcccaaagg cggcggtaat tgtcagacac tccttcgtga agtcgtccac gcaggtcagg
      961 cacttgatcc tgcgaccggt ggccagcgcg tccatgacaa aatccatcgg ccaggtcagg
     1021 ttgggcgcct ccgggcggag cagcggcaga cgttctgttg ccagaccttt acgacgtcgt
     1081 ctgcgtttta cacccagccc gttaagatgg taaagacggt acacgcgctt gtgattaaca
     1141 tgaaggcctt cacggcgcag taactgccag atgcgtcggt agccaaaacg cctgcgctcc
     1201 agtgccagct cagtgatgtg ccctgataaa tgcgcatcag ccgcgggacg ctgagcctca
     1261 tagcggcagg tcgacaggga caaacctgta agcctgcagg cacgacgttg cgacagaccg
     1321 gtcgcatcac acataaactc aacggcttcc cgtttctggt ctgtcgtcag tactttcgcc
     1381 ccagagccac ctgaagtgcc tccttatcca gcatggcttc ggcaagcagt ttcttgagtc
     1441 tggcattctc ttcctcaagc gacttaaggc gcttaacctc gggcacctcc ataccgccat
     1501 acttcttacg ccaggtgtaa aagatggcgt cggaaatggc gtgcttacgg cagagctcac
     1561 gggcagaaac cccggcttcg gcctcgcgga gaatactgat gatctgttcg tcggaaaaac
     1621 gcttcttcat gaggatgtcc tcatgtggct tatgaagaca ttactaacat cacggtgtat
     1681 taaccaacgg ggagcaggtc actccgtgta tgccccggtt ttttcgcgct gtcgctgtct
     1741 aaactggctg taaaaattta cgcctactgg gatgggctcg cagtaggttc attaaccaat
     1801 tattaaatgc tcatgttatc aaacatggtt cttttttttt attattatct tcctttctat
     1861 tgtgtaatat gcaacgcagc ctattactat cgaagtaaca aaacatagaa acttttcagg
     1921 tgcgttgtta tgagttagtt tagaaaatgc aaacacagcg taaaggtgtg ttagataaat
     1981 ggagtatgaa gctgttccta tataaatcaa tgctttcttt agaggtattt tatctacact
     2041 tacctttgat gttatgtata tgactacgca tgctggaatc ccagatgtta gaaatgtaat
     2101 gagttggtag ttattatatg gctcaggtaa gtgaatgtgg cctataatgg tgcaactcgt
     2161 gattattatt gctattgata gcaatatagg gaatgcattt tttttgtatg tgtacccatt
     2221 gtacgccaat ccaattaagg agccaagtat gaagtctaat attagatagt taccaatcat
     2281 gtgatgaata gaattatctt tccagcccgc taatccgccg ttaactccac ccgctaaaaa
     2341 aaataaaaca agcatggtga atgaagtgga taagataaca ttccttgccc ctttgaatat
     2401 gtacatgcac aatgaaaata ctatgtaaaa atataattca tacgttaatg tccagcaaac
     2461 aaagtttatc ctatcatagt taccatgaat agttgggagg agggttatgt tatgaaaaat
     2521 atcagatgca cttatgtttt tatagaaata tgaaaaaata ataagaggca gtgataacac
     2581 aaggtaaggt ggatatattc ttaatattct gctttttata aattgatacc ctgttctgtt
     2641 tttgcggtat gtaatagtca ttaaataacc gcttagcaca aaaaacagat caacaccaaa
     2701 gatacccgaa gagccaatta caggtacgct tgcgccataa atgtgtgtaa gaactactga
     2761 acaggctgct atggctcgaa taacttgtag tgactcgata cttttattca tgaatttaca
     2821 tactgaaatg ggttattgag gtattttact ggaaaaaact ggatgtatca ctaattactt
     2881 gtggtgatga cttcaactta ctgatagtgt tttatgttca gataatgccc gatgactttg
     2941 tcatgcagct ccaccgattt tgagaacgac agtgacttcc tgcccagcct tgccagatac
     3001 tgcctcagat tcaggttatg tcgctcaatg cgctgcgtat atcgcttgct gataacgtgc
     3061 agttctccct tcaggcgtga ttcatacagc ggccagccat ccgtcatcca taccacgacc
     3121 tcaaaggccg acagcaggct cagaagacgc tccagtgtgg ccagagtgcg ttcaccgaac
     3181 acgtgcgcca caaccgtcct ccgtatcctg tcatacgcgt aaaacaacca gcgctggcgt
     3241 gatttagcgc cgacgtaacc ccactgttcg tccatttccg cgcaaacaat gacgtcactg
     3301 cccggttgta tgcgtgagtt taccgactgc ggcctgagtt ttttaagtgt cgtaaaatcg
     3361 tgttgaggcc aacgcccata atgcgtgcac tggcgcgaca tccgacgcca ttcatggcca
     3421 tatcaataat tttctggtgt gtaccgggct gagaggcggt gtaagtgaac tgtagctgcc
     3481 atgttttacg gcagtgagag cagagatagc gctgatgtcc ggcagtactt ttaccgttac
     3541 gcaccacgcc ttcagtagct gagcaggaag gacaactgat ggagatggaa gccacgggag
     3601 cacctcaaaa acaccatcgt acactaaatc agtaagttgg caccattacc gtgatttgtt
     3661 atatacccac cacgggggca ttagtttttt tatttttttg atgatatgta caaaatcagt
     3721 ggttaggaat aatcttagca gccttaacaa acacatacaa aaacagcggt tatgtttcat
     3781 ctcaccacaa taagtaacga cagtatcatg ctaaaaaaag atgacattat ctttatgaaa
     3841 tcataacagg ctgtttttat taaattaatc aggcatttat ggactggatt tcacttgcta
     3901 tcgccattgc tctacttgtt gtagctgtct actcaggaat ctcatacttc agagaaaaaa
     3961 aaacaatcag ccgtgtattt aggcgatagc gccctattta ccataatccc ttcttacgcg
     4021 caccgcgaaa acggcctcag ggcgtttctg gtggttaacg ggcaaaaggt ggtggcaagt
     4081 acaccgaaaa ccgccagaaa cgcgcctgat ttattttacg catatgcatg gctatgcaca
     4141 ccaccgcata gccggtgatt tcggccgaga aaagggttct ggacgttaaa agcataaaag
     4201 aacgggccat ccaggcccgt caaatgagga caactctgta aaaggaccgt cgcacctggt
     4261 gcggtgccgg cgacaagaaa aagatagctc atatcagccc ctcaccggag aaaaatattt
     4321 cctggccacc tcaaccgcca cacgctgtgt tcccgttaac tcgcgcagat aacgcaggaa
     4381 catcctgttt tcctctgcat gcgcctcctc actgccctgt gcgtggtatt ccactatcgc
     4441 gttcaccacg gactcaccga cctgcatcgt ctgacctcat ctgcactttc atcacaccgt
     4501 ttcagatccc gcctcctcgc catgatgtga aacggtgacc gggcaggcaa attttttgtc
     4561 tgtccggtca ccgttggcgg ggtccggggc ggtcagtccc ggtgtttttt attccgccaa
     4621 atcagcacct gcgacacccg ctatgtacga cgcataaaat gcgcctgtac atagcgggtg
     4681 tcgctattaa actgagtcag gcgcaacatt taaaaaacaa cattcacacg cacgaaaaac
     4741 ctggctagta tttagtcaaa ccatgaggag gcgcactctg acgcgcacct gcctaccggc
     4801 agcaggtaaa acagccgggt ccccccgggg atactgtacc ggacagccca atccgagaat
     4861 cctgagaaaa cagccaaaat ctgcgagacc ggcaatcagg tcaggccatt ccctggctta
     4921 agggacgttt ttacagccgc gagcagtccg ggcttaacca cgttacggtt taaacgaggg
     4981 cgatcggcgc aggccgcagt ggatttccac tggcgttctt acaactcaac accggagttc
     5041 ctgtggaatt tgataccgcc gccgaactgg ccgccctgca ggcgcagacc cgccgtatcc
     5101 gccaggtccg ctaccgtccc tcccgccttg accgctatac cggcgaactg ctgagcctgt
     5161 accaggccgg ggccagtgca gcggagctgc aacgctggct ccgggcccgg agaatcaaag
     5221 tcgtgttgtc cacggttacc cgctggctgg aaaaaaatgg ctaaattcgc cagcgctgcc
     5281 cgccaggcgg ctggcgtgat aaaaccactc cagggaaata ttctgcgctc tgtcggtacg
     5341 gtccggaatt acgagcaggg actgacccgc gtcgcagagt ggatgaaatt taaccgcatc
     5401 aaccacggtg atttacgcac actgacaccg gcgcaggcca tcacctacct ggagcagcgc
     5461 ggggagcagg tcggccagaa aacgctcgat atggaacgcc aggccatcca ggccatgatg
     5521 atccacgtga cgcagaccct ccctgccggc gaacacctgc cggtggtgaa atcggagcag
     5581 gcacagatcc tgagcagccg ggcctacagc ccggaccagg tggccctgat tgccgcagca
     5641 caacagcccg ccaacgccct ggccaccgcg atcgccctgg ccgccggatt a
//
