LOCUS       NZ_CP079639             8514 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC21_AI1646PC
            plasmid pAI1646P_P3, complete sequence.
ACCESSION   NZ_CP079639
VERSION     NZ_CP079639.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20130521
            Assembly: GCF_019317125.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC21_AI1646PC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8514)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC21_AI1646PC
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 58.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:04:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,587
            CDSs (total)                      :: 5,461
            Genes (coding)                    :: 5,315
            CDSs (with protein)               :: 5,315
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 65 of 146
            Pseudo Genes (incomplete)         :: 82 of 146
            Pseudo Genes (internal stop)      :: 22 of 146
            Pseudo Genes (multiple problems)  :: 20 of 146
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079639.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC21_AI1646PC"
                     /isolation_source="Laryngeal suction"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pAI1646P_P3"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2016"
                     /collected_by="Christian medical college"
     gene            join(6420..8514,1..911)
                     /locus_tag="KW557_RS27740"
                     /old_locus_tag="KW557_27705"
     CDS             join(6420..8514,1..911)
                     /locus_tag="KW557_RS27740"
                     /old_locus_tag="KW557_27705"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WP_001143760.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGTLGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            1768..1992
                     /locus_tag="KW557_RS27745"
                     /old_locus_tag="KW557_27710"
     CDS             1768..1992
                     /locus_tag="KW557_RS27745"
                     /old_locus_tag="KW557_27710"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071653266.1"
                     /translation="MSNKDSLERKVTPRKNEPVFPMALRVGIAEDICFLELVDFPEKES
                     GRVFYSIALTKKHAKNLIDGLNKFVESGD"
                     /name="hypothetical protein"
     gene            1994..2638
                     /locus_tag="KW557_RS27750"
                     /old_locus_tag="KW557_27715"
     CDS             1994..2638
                     /locus_tag="KW557_RS27750"
                     /old_locus_tag="KW557_27715"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044816498.1"
                     /translation="MKYINHCDSEDYYITNDSIDLGVRYDVSDYNKLELNDLRDPDLFK
                     VLGRAFVVVENSVWESWNDDVKTLDCSGDGYESILKHYESLELSGEFLLFFKKIRSAIS
                     VYNREYEFCTGDLEPSLQAIVQLIRYAPELAAKKDAQIYFDKKSECFGLIYKQTKKSKG
                     TLNLIVKTNNEIIFSFVRKVSGVVKVTGRAYIGDSLDNSIAIKSLFKMMEW"
                     /name="hypothetical protein"
     gene            2638..3114
                     /locus_tag="KW557_RS27755"
                     /old_locus_tag="KW557_27720"
     CDS             2638..3114
                     /locus_tag="KW557_RS27755"
                     /old_locus_tag="KW557_27720"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_077861907.1"
                     /translation="MTVLSTLNNLKSIPCINAFTGRANNSYLWRYVMEDHKDEFGATTI
                     KCFYLREEISEDYVSIYHSNKDYYEGMVRDVHKRIPVGKTKPAIYLSFDAAEVIEQVNK
                     PDKCIDIKDEGYPHLGLWYLFDINDEFTLIEVTTTILELSSFFQRNEGGVVVPL"
                     /name="hypothetical protein"
     gene            complement(3321..3434)
                     /locus_tag="KW557_RS28080"
     CDS             complement(3321..3434)
                     /locus_tag="KW557_RS28080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071653267.1"
                     /translation="MHEPPVQSDCCAFSGNYQLESNPERHDEAPLAAATGN"
                     /name="replication initiation protein"
     gene            3850..4041
                     /locus_tag="KW557_RS27760"
                     /old_locus_tag="KW557_27725"
     CDS             3850..4041
                     /locus_tag="KW557_RS27760"
                     /old_locus_tag="KW557_27725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_044816495.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSGLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4041..4463
                     /locus_tag="KW557_RS27765"
                     /old_locus_tag="KW557_27730"
     CDS             4041..4463
                     /locus_tag="KW557_RS27765"
                     /old_locus_tag="KW557_27730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_044816494.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKNTKVVD
                     TSELIRVFGSLYGGGDTVKHDVKGDAGATGVNISGLHDLQHKIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKLEPSPQVHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4656..5516)
                     /locus_tag="KW557_RS27770"
                     /old_locus_tag="KW557_27735"
     CDS             complement(4656..5516)
                     /locus_tag="KW557_RS27770"
                     /old_locus_tag="KW557_27735"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     gene            complement(5699..6256)
                     /locus_tag="KW557_RS27775"
                     /old_locus_tag="KW557_27740"
     CDS             complement(5699..6256)
                     /locus_tag="KW557_RS27775"
                     /old_locus_tag="KW557_27740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001235713.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     ARG             complement(4660..5516)
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(3322..3729)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.39"
                     /coverage="99.0"
                     /evalue="1.26e-177"
ORIGIN
        1 caatcaatgc cggaccgaac cgcaaatact ttggtaataa cagagggatc acctggtaca
       61 actttgtgtc cgatcagtat tccggctttc atggcatcgt tataccgggg acgctgaggg
      121 actctatctt tgtgctggaa ggtcttctgg aacaggagac cgggctgaat ccaaccgaaa
      181 ttatgaccga tacagcaggt gccagcgaac ttgtctttgg ccttttctgg ctgctgggat
      241 accagttttc tccacgcctg gctgatgccg gtgcttcggt tttctggcga atggaccatg
      301 atgccgacta tggcgtgctg aatgatattg ccagagggca atcagatccc cgaaaaatag
      361 tccttcagtg ggacgaaatg atccggaccg ctggctccct gaagctgggc aaagtacagg
      421 tttcagtgct ggtccgttca ttgctgaaaa gtgaacgtcc ttccggactg actcaggcaa
      481 tcattgaagt ggggcgcatc aacaaaacgc tgtatctgct taattatatt gatgatgaag
      541 attaccgccg gcgcattctg acccagctta atcggggaga aagtcgccat gccgttgcca
      601 gagccatctg tcacggtcaa aaaggtgaga taagaaaacg atataccgac ggtcaggaag
      661 atcaactggg cacactgggg ctggtcacta acgccgtcgt gttatggaac actatttata
      721 tgcaggcagc cctggatcat ctccgggcgc agggtgaaac actgaatgat gaagatatcg
      781 cacgcctctc cccgctttgc cacggacata tcaatatgct cggccattat tccttcacgc
      841 tggcagaact ggtgaccaaa ggacatctga gaccattaaa agaggcgtca gaggcagaaa
      901 acgttgctta acgtgagttt tcgttccact gagcgtcaga ccccagttcc tgaaccttcc
      961 caaaaatcgc cagatctctg aacgctcctc accactaaaa atatccacac cccacgcaaa
     1021 attaaaattt tgcagatttg tattttatat cagtagttta cgttctataa atttcgtttt
     1081 tttaatttta tttatacaga tccgactcta tgacgactcg tcatagagtc cccggcgagt
     1141 cgtcatagag tcggtaacga ctcaggacta taaaaacaat aagttaacaa cgacccgact
     1201 ctagacccga ctccccgttt ggttgttttt tagacgtgac gactctgggt acgactctag
     1261 tgaagagtcg ccggtgaatc gtgtggtgag tcgtccggcg acttgccata acgtgattgc
     1321 cggccgtcga cgaacccgat tcctgaacgt gctgaaaacc ggttttccgg ctgcgccggc
     1381 aacgcttcac ggccacgcaa taaattgcgt ccccgttcac cgttttcgga tgggaagaac
     1441 gccggaaaca ggacttaccc tggctgaatg gcgtgaattt tcgcgctatg cacttgcgtg
     1501 catactcatg catgccgtaa aaacagagcc tgcgcgtttc tggcgggttt tcgggtggtt
     1561 tgttgcctat tttaccggtt tcccgtcaga aacgccctga ggccgtttta gcggtgcgta
     1621 caattttggt gttatgttaa atcatggcca caaaattcca taaagtgacc aatgtaatgt
     1681 taaatgagca ttgtggatga aagatgttgt tttatggtat tattttttat gttctaaact
     1741 taaatttatt tctatcgggg tattataatg agcaataaag attctttaga aagaaaagta
     1801 acaccaagaa aaaatgaacc tgtattcccg atggctctaa gagttgggat agctgaagat
     1861 atttgttttt tagagttagt ggactttcct gaaaaagaat caggaagggt tttttattcc
     1921 atagctctaa ccaaaaaaca tgcaaagaac cttatcgatg gattgaataa attcgttgag
     1981 tcaggtgatt agtatgaaat acattaacca ctgcgatagt gaagattatt atattactaa
     2041 tgatagcatt gatctcggtg ttcgttatga tgtttctgat tataataagt tagagctaaa
     2101 tgatttaaga gatccggatt tgtttaaagt tttgggtcga gcatttgttg ttgttgagaa
     2161 ttctgtttgg gagtcttgga atgatgatgt taaaacatta gattgctctg gtgacggtta
     2221 tgagtcaatt ttaaaacatt acgaatcatt agaattatca ggtgaattct tattgttttt
     2281 taaaaaaata agaagtgcca tttctgttta taatagagaa tacgagttct gcactggtga
     2341 cctagaacca tctttgcagg ctattgttca gttgattagg tatgcccctg aacttgctgc
     2401 aaaaaaagac gctcagattt actttgataa aaaatcagag tgttttgggc ttatatataa
     2461 acaaactaag aaaagcaaag gcacgctaaa tttaatagtg aaaacaaata acgagattat
     2521 tttttccttt gttagaaaag tttctggagt ggttaaggtt acaggtaggg catatattgg
     2581 cgatagcctt gataactcca ttgctatcaa atcattattt aaaatgatgg agtggtgatg
     2641 actgtgttat caactttaaa taacctcaaa tcaatccctt gtattaatgc atttacgggc
     2701 agagcaaata attcatattt atggcgttat gttatggaag atcataaaga tgagtttggc
     2761 gctacaacta ttaagtgttt ttatcttagg gaggaaattt cagaggatta tgtgtctatt
     2821 tatcattcaa ataaagatta ttatgaagga atggtaaggg atgtacataa aagaatccct
     2881 gtagggaaaa ccaaacctgc catatatctc tcatttgatg cagcggaagt gatcgagcaa
     2941 gttaacaaac cagataaatg tattgatata aaggacgaag gttaccctca cttgggtttg
     3001 tggtatttat ttgatattaa tgatgaattt actttgattg aggtgactac aactattttg
     3061 gaattatcct ctttttttca aagaaatgaa ggtggcgtgg ttgttcctct ttagcgaaaa
     3121 ccgtcttgac gtcatcaagt gtgacctagt actcttcttg aaaaagaaaa accgcctttg
     3181 cggggcggtt tttcttaggt tctaagagtt tgcggctcta tgaaccaagg taactggcta
     3241 gttagaggag cgcagtaacc aaaatcgttc tttcagttta gccttaactg gcacataact
     3301 tcaagactaa ctcctctaaa tcagttacca gtggctgctg ccagtggcgc ttcgtcgtgt
     3361 ctttccgggt tggactcaag ttgatagtta ccggaaaagg cgcagcagtc ggactgaacg
     3421 gggggttcgt gcatacagtc cagcttggag cgaactgcct acccggaact gagtgtcagg
     3481 cgtggaatga gataaacgcg gccataacag cggaatgaca ccggtaaacc gaaaggcagg
     3541 aacaggagag cgcacgaggg agccaccagg gggaaacgcc tggtatcttt atagtcctgt
     3601 cgggtttcgc caccactgat ttgagcgtca gatttcgtga tgcttgtcag gggggcggag
     3661 cctatggaaa aacggccgct gtgcggcctc tccttctccc catcacgcga tcgctcggtt
     3721 ttctcgagct tttatacgca tgctcctgcc gctcgccgca gccgaacggc aggagcgtag
     3781 cgactgagtg agcgaggaag cggaaaagag aacatcccta cactgagcac tgacgctctg
     3841 aggcctctta tgacgaaaca acagaaaaca gcgctgaaca tggctaaatt catccagaat
     3901 cagtctctgc tcctgctgga gaagcttaac gagcttgatc ttgatgtaga ggccgacctt
     3961 tgtgagaaac tccacgatga tgcggagcat cttttccgta cactgtcttc gggactggat
     4021 gaattacagg atggaaactg atggccagag tgtctattag tgaagcggct cggcttgtta
     4081 aggttagtcg ccccacaatt tacaagatga ttaacagtgg gaaactaagt tacacctctg
     4141 ttgtaaagca tgggaaaaat actaaagttg ttgatacatc agagcttatt agggtttttg
     4201 gctcgcttta tggtgggggc gacactgtaa aacatgatgt aaaaggtgac gcaggcgcta
     4261 caggggttaa catctctggt ttacatgatt tacagcataa aattgctttg cttgaagctg
     4321 aaaacgatgg tcttaaaggg gctgttaaag ctcgtgatga acatatcgat tctcttcgcc
     4381 aggctatgca gcttctggaa cacaagctcg aaccctctcc acaggttcat tctccgtggt
     4441 ggaaattctg gaagaaatcc tgaccccgtc atgcttcagt tttcctctcc ggccattccg
     4501 gggagttcgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     4561 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     4621 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     4681 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     4741 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     4801 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     4861 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     4921 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc
     4981 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     5041 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     5101 gttgtcagaa gtaagttggc agcagtgtta tcactcatgg ttatggcagc actgcataat
     5161 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     5221 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
     5281 aataccgcac cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     5341 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     5401 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     5461 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgaa aatgttgaat actcatactc
     5521 ttcctttttc aatattattg aagcatttac cagggttatt gtctcatgag cggatacata
     5581 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     5641 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     5701 acgaggccct ttcgtcttca agaattttat aaaccgtgga gcgggcaata ctgagctgat
     5761 gagcaatttc cgttgcacca gtgcccttct gatgaagcgt cagcacgacg ttcctgtcca
     5821 cggtacgcct gcggccaaat ttgattcctt tcagctttgc ttcctgtcgg ccctcattcg
     5881 tgcgttctag gatcctccgg cgttcagcct gtgccacagc cgacaggatg gtgaccacca
     5941 tttgccccat atcaccgtcg gtactgatcc cgtcatcaat gaaccggact gccacgccct
     6001 gagcgtcaaa ttcctttatc agttggatca tatcggcagt gtcgcggcca agacggtcga
     6061 gcttcttaac cagaatgaca tcaccttcct ccaccttcat cctcagcaaa tccagccctt
     6121 cccggtctgt tgaactgccg gatgccttat cggtaaatat acggtttgct ttcacacctg
     6181 cgtctttgag tgctctgacc tgaagatcaa gagactgctg actggttgag acccgagcgt
     6241 aaccaaaaag tcgcataaaa atgtacctta aatcgaatat cggacaactc atgtctatta
     6301 ttacaaattt acgatttaat agacatatta atgtaacagt tttacgatgt ccgataattt
     6361 ataacatttc gtacggttgg aaaaatgtta ctaaatgccc gtcaggcagg gaggccgata
     6421 tgcccgttga ctttctgacc actgagcaga ctgaaagcta tggcagattc accggtgaac
     6481 cggatgagct tcagctggca cgatattttc accttgatga agcagacaag gaatttatcg
     6541 gaaaaagcag aggtgatcac aaccgtctgg gcattgccct gcaaattgga tgtgtccgtt
     6601 ttctgggcac cttcctcacc gatatgaatc atattccttc cggcgtccgg cattttaccg
     6661 ccagacagct cgggattcgt gatatcaccg ttcttgcaga atacggtcag agggaaaata
     6721 cccgccgtga gcatgcagcg ctgatacgtc agcactatca gtatcgtgaa tttgcctggc
     6781 cctggacatt tcgccttacc cgtcttttat atacccggag ctggataagc aacgaacgtc
     6841 ctggcctgct tttcgatctg gcgacagggt ggcttatgca acatcgtatt attctccccg
     6901 gagccactac gctgacccgg ttgatttcag aggtaaggga aaaggcgacg ttgcgcctgt
     6961 ggaacaaact ggcactgata ccgtcagccg aacagcgttc acagctggag atgctgctgg
     7021 ggccaactga ttgcagccgc ctgtctttac tggaatcact gaaaaagggc cctgtgacca
     7081 tcagtggtcc ggcgtttaat gaagcaattg aacgctggaa aactctgaac gattttggcc
     7141 tgcatgctga aaacctgagt acactcccgg ctgtgcgcct gaaaaatctc gcacgttatg
     7201 ctggtatgac ttcggtgttc aatattgcca ggatgtcacc gcagaaaagg atggcggttc
     7261 tggttgcctt tgtccttgca tgggaaacgc tggcgctgga tgatgcattg gacgttctgg
     7321 acgccatgct ggccgttatc atccgtgacg ccagaaagat tgggcagaaa aaacggctcc
     7381 gctcgctgaa ggatctggat aaatctgcat tggcgctcgc cagcgcatgt tcgtacctgc
     7441 tgaaagaaga aacaccggac gaatcgattc gtgctgaggt gttcagctac atcccaaggc
     7501 aaaagctggc tgaaatcatc acgcttgtcc gtgaaattgc ccggccctca gacgataatt
     7561 ttcatgaaga aatggtggag cagtacgggc gcgttcgtcg tttcctgccc catctgctga
     7621 ataccgttaa attttcatcc gcacctgccg gggttaccac tctgaatgcc tgtgactacc
     7681 tcagccggga gttcagctca cggcggcagt tttttgacga cgcaccaacg gaaattatca
     7741 gtcggtcatg gaaacggctg gtgattaaca aggaaaaaca tatcacccgc aggggataca
     7801 cgctctgctt tctcagtaaa ctgcaggata gtctgaggcg gagggatgtc tacgttaccg
     7861 gcagtaaccg gtggggagat cctcgtgcaa gattactaca gggtgctgac tggcaggcaa
     7921 accggattaa ggtttatcgt tctttggggc acccgacaga cccgcaggaa gcaataaaat
     7981 ctctgggtca tcagcttgat agtcgttaca gacaggttgc tgcacgtctt tgcgaaaatg
     8041 aggctgtcga actcgatgtt tctggcccga agccccggtt gacaatttct cccctcgcca
     8101 gtcttgatga gccggacagt ctgaaacgac tgagcaaaat gatcagtgat ctactccctc
     8161 cggtggattt aacggagttg ctgctcgaaa ttaacgccca taccggattt gctgatgagt
     8221 ttttccatgc tagtgaagcc agtgccagag ttgatgatct gcccgtcagc atcagcgccg
     8281 tgctgatggc tgaagcctgc aatatcggtc tggaaccact gatcagatca aatgttcctg
     8341 cactgacccg acaccggctg aactggacaa aagcgaacta tctgcgggct gaaactatca
     8401 ccagcgctaa tgccagactg gttgattttc aggcaacgct gccactggca cagatatggg
     8461 gtggaggaga agtggcatct gcagatggaa tgcgctttgt tacgccagtc agaa
//
