LOCUS       NZ_CP079633             6200 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC18_CMC307MC
            plasmid pCMC307M_P4, complete sequence.
ACCESSION   NZ_CP079633
VERSION     NZ_CP079633.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20130509
            Assembly: GCF_019317085.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC18_CMC307MC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC18_CMC307MC
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 67.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:12:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,951
            CDSs (total)                      :: 5,829
            Genes (coding)                    :: 5,652
            CDSs (with protein)               :: 5,652
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 69 of 177
            Pseudo Genes (incomplete)         :: 110 of 177
            Pseudo Genes (internal stop)      :: 24 of 177
            Pseudo Genes (multiple problems)  :: 22 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079633.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC18_CMC307MC"
                     /isolation_source="Endotracheal aspirate"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pCMC307M_P4"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2018"
                     /collected_by="Christian medical college"
     gene            complement(join(5493..6200,1..96))
                     /gene="aph(3'')-Ib"
                     /locus_tag="KW554_RS29505"
                     /old_locus_tag="KW554_29490"
     ARG             complement(5494..6200)
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.57"
                     /coverage="88.01"
     gene            complement(157..972)
                     /gene="sul2"
                     /locus_tag="KW554_RS29510"
                     /old_locus_tag="KW554_29495"
     ARG             complement(161..972)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(1432..1590)
                     /locus_tag="KW554_RS29985"
     CDS             complement(1432..1590)
                     /locus_tag="KW554_RS29985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            2161..2310
                     /locus_tag="KW554_RS29515"
                     /old_locus_tag="KW554_29500"
     CDS             2161..2310
                     /locus_tag="KW554_RS29515"
                     /old_locus_tag="KW554_29500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            complement(3055..3495)
                     /locus_tag="KW554_RS29520"
                     /old_locus_tag="KW554_29505"
     CDS             complement(3055..3495)
                     /locus_tag="KW554_RS29520"
                     /old_locus_tag="KW554_29505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            3713..4102
                     /locus_tag="KW554_RS29525"
                     /old_locus_tag="KW554_29510"
     CDS             3713..4102
                     /locus_tag="KW554_RS29525"
                     /old_locus_tag="KW554_29510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            4146..>4550
                     /locus_tag="KW554_RS29530"
                     /old_locus_tag="KW554_29515"
                     /pseudo=""
     CDS             4146..>4550
                     /locus_tag="KW554_RS29530"
                     /old_locus_tag="KW554_29515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(4657..5493)
                     /locus_tag="KW554_RS29535"
                     /old_locus_tag="KW554_29520"
     CDS             complement(4657..5493)
                     /locus_tag="KW554_RS29535"
                     /old_locus_tag="KW554_29520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     ARG             complement(4661..5493)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          1953..2126
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(1424..2029)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gtcaccacgt cgaaaaacaa aatcaccaga ttctccgcct ctgacaggca accagtcaga
       61 atgcgattca ccaaaaaaaa tattagttcg attcaatgga ggttccttca gttttctgat
      121 gaagcgcgaa tatagagaaa tatcccgaat gtgcagttaa cgaattcttg cggtttcttt
      181 cagcgccgcc aataccgcca gcccgtcgcg caaggggcgc ggctcgtgtg tgcggatgaa
      241 gtcagctcca cctgcggcgg cggcaagctc tgcagcgagt gtcgcggccc cgacatcccc
      301 cggaccacgg cctgtgagcg cgcgcagaaa ggatttgcgc gaaacagaca gaagcaccgg
      361 caaatcgaag cgcagccgca attcatcgaa ccgcgccagc accgagagcg aggtttcggg
      421 agcagccccc agaaaaaacc ccatgccggg atcaaggaca aggcggttgc gtttgatacc
      481 ggcacccgtc agcgccgcga tgcgcgcgtc aaagaacgcc gcaatgtgat ccatgatgtc
      541 gccagcgggt gcctcgcgcc gatctgcctg cccgtcttgc accgaatgca taacgacgag
      601 tttggcagat gatttcgcca attgcggata gaacgcagcg tctggaaaac cgcgaatatc
      661 attgagatag gccacaccac gcgacaaggc ataggcttgc gtcgcgggtt gataactgtc
      721 gagcgagacg ggaatgccat ctgccttgag cgcgtccagc accggcgcga tacgcgcgat
      781 ttctgtgtcg gacgaaacag gcgcggcgtc gggattgctg gatgccggac cgaggtcgat
      841 cacatctgcc ccctcggcca tcagcttacg cgcctgcgca atggctgcgt ctggcgccag
      901 ataccggcct ccatcggaga aactgtccga ggttatgttg acgatgccga aaatgatgag
      961 cgatttattc atgggggctt ctataataat aataatcgag catgagtctc atacggatgc
     1021 tcgggtcgaa agggaatccc caggcgagta acctgtttgc ggtgatccgt ttcatgatga
     1081 tgtatctgta gcattttgga aataaatata tcatctctca ataagtagat atatttatct
     1141 tttttgcttc tttggtgctg caagtcacca atacgaggga aacctgttac ggagcggttt
     1201 aaccgtaatc aacagtgact ggtgtcatgt tcggaagatt aattgatagt aatctacaaa
     1261 ggatcttctt gagatccttt tcaatgaatc gtaatctctt gctctgtaaa cgaaaaaacc
     1321 gcctggggag gcggtttgtt cgaaggttaa gtcagttggg gaactgctta acccggtaac
     1381 agggttcacg agaccgccat taccaaaact gttctttcag tgtagccgca gtcaggcctc
     1441 tacttcaaga cctcttaatc tatcttctcg tgcactcctg ttactaatgg ctgctgccag
     1501 tggcgttttg tcgtgtcttt ccgggttgga ctcaagttga tagttaccgg acaaggcgca
     1561 gcggtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgccttcac
     1621 ggaactgagt gtcaggcgtg gaatgagaaa ccgcggccat aacagcggaa tgacaccggt
     1681 aaaccgaaag gcaggaacag gagagcgcac gagggagcta ccagggggaa acgcctggga
     1741 tctttatagt cctgtcgggt ttcgccacca ctgatttgag cgtcagattt tgcgatgctt
     1801 gtcagggggg cggagcctat ggaaaaacgg cttcgccgtg gccttatctc ttctttgcta
     1861 agtgtcttcc tggtatcttc caggaaattt ccgtccccgt ccgtaagccg gtaccgctcg
     1921 ccgcagccga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atctcctgta
     1981 tcgcatattc tgctgacgcg ccggtgctgc cttttttctc ctgccacatg aagcacttca
     2041 ctgattttcg caatcgtggc aacatagtaa gccagtatac actccgctag cgctacatga
     2101 ctggttcagg gctgcgcccc gaaacccgct aacaacctct cagctactgg atatcacccc
     2161 atgccatctg aatatgatga acaactggat tcgactgata cgcagcttcc cggcgaatat
     2221 gacctttttg atttaatggc gcaggttact gaagagaacc tgcatgcccc tgtggatttt
     2281 gggtcgcctg tcggccggga agcgttatag ctttttcgct tgcccgcgta cgtggtaata
     2341 ctccggtatt accttttatt tctttatttt catgtaatta cagtggattt ttaatacccg
     2401 ggtattactc gtgaaaacga aatattcctt ttttttcttt taattcatgc agttgcgatg
     2461 aaaatcgcgg ctgcgttagg tgtatgacca ttttgaggtt gaaaaataat caaccatggc
     2521 gggtgattta cgcaggttaa ggggagatcg tgacaaaaaa agcgttcctc actgtatcgt
     2581 tcatgcctgc atgaacgggt tattcttcct gactacagaa ttacatcgct ccttcagttc
     2641 atacatcact tttacatcac ttatacatca cttttacatc attccttcag ctcatacgtc
     2701 actttcatcc tctgtgccat ttgtacatca cccccttcct gaacgtgctg acagcgggtt
     2761 ttccggctgc gccggcaacg cttcacggcc acgcaataaa ttgcgtcccc gttcaccgtt
     2821 ttcggatggg aagaacgccg gaaacaggac ttaccccggc tgaatggtgt gaatttccgc
     2881 gctatgcact tgtgcgcata ctcatgcatg ccgtaaaaac agagcctgcg cgtttctggc
     2941 gggttttcgg gtggtttgtt gcctgtttta ccggtttccc gtcagaaacg ccctgagggc
     3001 ctctcaggcg gtgctgataa cagatgttat ggtaaatcag agtcacctgc actgttactc
     3061 tgaaccggag cgaaacagcc gcgaccagaa tccccgcttt ttttcctgtg ccagcgcgtc
     3121 agccagacgg ctaatttcgg cctgtagttg ttccctctcc tcatttcgca ggcggttttc
     3181 tgcttcccgc gccatcttgt cttcaagcat tagggtaaca gtctgctgca gagcgtccag
     3241 tttctgctga atgacgtcta gctgagcatt ttccgcctgg ctaacgacag atttggagtg
     3301 tccctccttg tgcctttttg gtgtctcaga agtgcgcagc tcgccatagg cccgaatcag
     3361 ttcactggtc tcgaactccc ggcgatcatt ttttcctgtg cgatagctga caaggccttt
     3421 agccatgtca cgatataatt gcgagcgcga ctttcctgtt aatttcagtg tctcttgaac
     3481 tgtgtgccat gccataaaag tgacagtgtc tcctgtgtgt accgtattgt aacagcagaa
     3541 catattactg ttttaacaca aaaggtctga ggctgcccgt gaggcgtgaa aaatacacaa
     3601 aaaacctgtt gacggtgtgg ttttgacggg cgtagtattt cgaatactgt atataaacac
     3661 attgttataa atacagtttt tgctgttttt aaaagtcaaa gaggataatt ttatgagtac
     3721 agagttaaat agcggtactg tcggtggttt tggtcacatg ggaccaaatg acccctacaa
     3781 tgatggtcat agcggctctg gaagttcggg aggtagtttt aacggtggtg gtaatggtgg
     3841 ccatgataat agtagtggtt catcttatac tgcatccttt ggtctcatca ctggccgaca
     3901 cccgataact gtcccaggta cgaagaatat taattctgtc gatctttctg ctgtcattaa
     3961 taacattata catcatgaag gtgcacgcaa taacatgtac ctagatactg cgaacaatgt
     4021 aagagtggga gtgacgggca ctggctggca atgtctagca acggcaggca tttcggctga
     4081 gggtaaaaga actttccgct aagcgataga ctgtatgtaa acacagtatt gcaaggacgc
     4141 ggaacatgcc tcatgtggcg gccaggacgg ccagccggga tcgggatact ggtcgttacc
     4201 agagccaccg acccgagcaa acccttctct atcagatcgt tgacgagtat tacccggcat
     4261 tcgctgcgct tatggcagag cagggaaagg aattgccggg ctatgtgcaa cgggaatttg
     4321 aagaatttct ccaatgcggg cggctggagc atggctttct acgggttcgc tgcgagtctt
     4381 gccacgccga gcacctggtc gctttcagct gtaagcgtcg cggtttctgc ccgagctgtg
     4441 gggcgcggcg gatggccgaa agtgccgcct tgctggttga tgaagtactg cctgaacaac
     4501 ccatgcgtca gtgggtgttg agcttcccgt ttcagctgcg tttcctgttt ggggtcgttt
     4561 gcgggaaggg gcggaatcct acgctaaggc tttggccagc gatattctcc ggtgagattg
     4621 atgtgttccc aggggatagg agaagtcgct tgatatctag tatgacgtct gtcgcacctg
     4681 cttgatcgcg gccgcgatag ctagatcgcg ttgctcctct tctccatccg cgttccaagc
     4741 tgcggaaagg cacccataag cgtacgcctg gtcgagcagg cgacgcggat cgacgtccag
     4801 cgcacgagag aatgcgtccg ccatctgtgc aatgcgtcta ggatcgagac aaaggtcgtc
     4861 tctgtcagcc ggatcgtaga acatattggc ggcgccaaag cccacttcac cgaccagacc
     4921 gacgggatct atcaccagcc agccgcgact ggagaacatg atgttttcat gatgcagatc
     4981 gccatgtagc ccacgcagtt ccgaggcatt gctcatcatt tgatcggcta taatcgccgc
     5041 gtggacgtag tcagtttgac aacctgcgtt ttgatcatcg cgcgcccgct gaaacaaagc
     5101 tgcaaagcga tcccggatcg ggagaagggc agaaggcagg ggttcctcag atgcggcata
     5161 cagcttcgcc attagttccg ctgcaatttc ggtcgcctgg tagtcgccgt gctcggcaac
     5221 gatgtgagag agcattcgct ccccggcata ttcgagcaac atcagattgt tctcacgacc
     5281 gagcaaccgg actgctcccc tcccattgcg ccataccaga tagtcggccc cgcgcagttc
     5341 atcagcaatg tcttctatag gtttcaatcc cttgacgatt gcaggagtcc cgtctggcaa
     5401 tgaaactttc caaacgaggc tggaaaaggt gtccgcaatg agaacaggtt gcgaaacgtg
     5461 ccaatgagca ggaaaaacag gcggcatgaa catcaacccc aagtcagagg gtccaatcgc
     5521 agatagaagg caaggcgttc gcggtcgggg gcttcgatcc ccaatacatt gaataggaca
     5581 gcgaaggcgc gctctgcttc atctggcgct gcccagttct cttcggcgtt agcaatcatg
     5641 agtgccaaat cggcatagcg atctgctgtt ccgagccgcc caaggtcgat cagacccgtg
     5701 cattgaagag ttttagggtc caccatgaag ttcggcatgc agggatcacc atggcaaaca
     5761 accatatcgg tgcgctcttg gtcgagccgc accggtagct ctcgttcgac acgagccaaa
     5821 agatcgagct gcggcgtact cttgtcctcg tccggtaaga agtcgggatt gacggcattg
     5881 cgggacacca catcaacggc gcgtccgaac attcgcgaca gcctgcgctc aaacggacat
     5941 tgatcaaccg ataggctgtg aacagcgcca agttgctgcc ccattgacgg ccacgctttg
     6001 agcaaatccg ctccagacag atcagccgcc ggtactcccg gaattgccgt tatcaccaag
     6061 catgcaccct cctgttcctc ctgccagttg atgacctcgg ggcaagccac acctcgacct
     6121 ttgagccaaa tgaggcggtc acgctctcca gcgagctcac cgcggcggga agcaggtgcg
     6181 attttcgcga aggcatgccc
//
