LOCUS       NZ_CP079628             6200 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC17_CMC307PC
            plasmid pCMC307P_P4, complete sequence.
ACCESSION   NZ_CP079628
VERSION     NZ_CP079628.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20130506
            Assembly: GCF_019317045.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC17_CMC307PC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC17_CMC307PC
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 91.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:12:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,951
            CDSs (total)                      :: 5,828
            Genes (coding)                    :: 5,651
            CDSs (with protein)               :: 5,651
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 69 of 177
            Pseudo Genes (incomplete)         :: 110 of 177
            Pseudo Genes (internal stop)      :: 24 of 177
            Pseudo Genes (multiple problems)  :: 22 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079628.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC17_CMC307PC"
                     /isolation_source="Endotracheal aspirate"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pCMC307P_P4"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2018"
                     /collected_by="Christian medical college"
     gene            join(5962..6200,1..>166)
                     /locus_tag="KW552_RS29505"
                     /old_locus_tag="KW552_29490"
                     /pseudo=""
     CDS             join(5962..6200,1..>166)
                     /locus_tag="KW552_RS29505"
                     /old_locus_tag="KW552_29490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(273..1109)
                     /locus_tag="KW552_RS29510"
                     /old_locus_tag="KW552_29495"
     CDS             complement(273..1109)
                     /locus_tag="KW552_RS29510"
                     /old_locus_tag="KW552_29495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(1109..1912)
                     /gene="aph(3'')-Ib"
                     /locus_tag="KW552_RS29515"
                     /old_locus_tag="KW552_29500"
     ARG             complement(1113..1912)
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(1973..2788)
                     /gene="sul2"
                     /locus_tag="KW552_RS29520"
                     /old_locus_tag="KW552_29505"
     ARG             complement(1977..2788)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(3248..3406)
                     /locus_tag="KW552_RS29985"
     CDS             complement(3248..3406)
                     /locus_tag="KW552_RS29985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            3977..4126
                     /locus_tag="KW552_RS29525"
                     /old_locus_tag="KW552_29510"
     CDS             3977..4126
                     /locus_tag="KW552_RS29525"
                     /old_locus_tag="KW552_29510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            complement(4871..5311)
                     /locus_tag="KW552_RS29530"
                     /old_locus_tag="KW552_29515"
     CDS             complement(4871..5311)
                     /locus_tag="KW552_RS29530"
                     /old_locus_tag="KW552_29515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            5529..5918
                     /locus_tag="KW552_RS29535"
                     /old_locus_tag="KW552_29520"
     CDS             5529..5918
                     /locus_tag="KW552_RS29535"
                     /old_locus_tag="KW552_29520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     ARG             complement(277..1109)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          3769..3942
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(3240..3845)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cgccgagcac ctggtcgctt tcagctgtaa gcgtcgcggt ttctgcccga gctgtggggc
       61 gcggcggatg gccgaaagtg ccgccttgct ggttgatgaa gtactgcctg aacaacccat
      121 gcgtcagtgg gtgttgagct tcccgtttca gctgcgtttc ctgtttgggg tcgtttgcgg
      181 gaaggggcgg aatcctacgc taaggctttg gccagcgata ttctccggtg agattgatgt
      241 gttcccaggg gataggagaa gtcgcttgat atctagtatg acgtctgtcg cacctgcttg
      301 atcgcggccg cgatagctag atcgcgttgc tcctcttctc catccgcgtt ccaagctgcg
      361 gaaaggcacc cataagcgta cgcctggtcg agcaggcgac gcggatcgac gtccagcgca
      421 cgagagaatg cgtccgccat ctgtgcaatg cgtctaggat cgagacaaag gtcgtctctg
      481 tcagccggat cgtagaacat attggcggcg ccaaagccca cttcaccgac cagaccgacg
      541 ggatctatca ccagccagcc gcgactggag aacatgatgt tttcatgatg cagatcgcca
      601 tgtagcccac gcagttccga ggcattgctc atcatttgat cggctataat cgccgcgtgg
      661 acgtagtcag tttgacaacc tgcgttttga tcatcgcgcg cccgctgaaa caaagctgca
      721 aagcgatccc ggatcgggag aagggcagaa ggcaggggtt cctcagatgc ggcatacagc
      781 ttcgccatta gttccgctgc aatttcggtc gcctggtagt cgccgtgctc ggcaacgatg
      841 tgagagagca ttcgctcccc ggcatattcg agcaacatca gattgttctc acgaccgagc
      901 aaccggactg ctcccctccc attgcgccat accagatagt cggccccgcg cagttcatca
      961 gcaatgtctt ctataggttt caatcccttg acgattgcag gagtcccgtc tggcaatgaa
     1021 actttccaaa cgaggctgga aaaggtgtcc gcaatgagaa caggttgcga aacgtgccaa
     1081 tgagcaggaa aaacaggcgg catgaacatc aaccccaagt cagagggtcc aatcgcagat
     1141 agaaggcaag gcgttcgcgg tcgggggctt cgatccccaa tacattgaat aggacagcga
     1201 aggcgcgctc tgcttcatct ggcgctgccc agttctcttc ggcgttagca atcatgagtg
     1261 ccaaatcggc atagcgatct gctgttccga gccgcccaag gtcgatcaga cccgtgcatt
     1321 gaagagtttt agggtccacc atgaagttcg gcatgcaggg atcaccatgg caaacaacca
     1381 tatcggtgcg ctcttggtcg agccgcaccg gtagctctcg ttcgacacga gccaaaagat
     1441 cgagctgcgg cgtactcttg tcctcgtccg gtaagaagtc gggattgacg gcattgcggg
     1501 acaccacatc aacggcgcgt ccgaacattc gcgacagcct gcgctcaaac ggacattgat
     1561 caaccgatag gctgtgaaca gcgccaagtt gctgccccat tgacggccac gctttgagca
     1621 aatccgctcc agacagatca gccgccggta ctcccggaat tgccgttatc accaagcatg
     1681 caccctcctg ttcctcctgc cagttgatga cctcggggca agccacacct cgacctttga
     1741 gccaaatgag gcggtcacgc tctccagcga gctcaccgcg gcgggaagca ggtgcgattt
     1801 tcgcgaaggc atgcccgtca ccacgtcgaa aaacaaaatc accagattct ccgcctctga
     1861 caggcaacca gtcagaatgc gattcaccaa aaaaaatatt agttcgattc aatggaggtt
     1921 ccttcagttt tctgatgaag cgcgaatata gagaaatatc ccgaatgtgc agttaacgaa
     1981 ttcttgcggt ttctttcagc gccgccaata ccgccagccc gtcgcgcaag gggcgcggct
     2041 cgtgtgtgcg gatgaagtca gctccacctg cggcggcggc aagctctgca gcgagtgtcg
     2101 cggccccgac atcccccgga ccacggcctg tgagcgcgcg cagaaaggat ttgcgcgaaa
     2161 cagacagaag caccggcaaa tcgaagcgca gccgcaattc atcgaaccgc gccagcaccg
     2221 agagcgaggt ttcgggagca gcccccagaa aaaaccccat gccgggatca aggacaaggc
     2281 ggttgcgttt gataccggca cccgtcagcg ccgcgatgcg cgcgtcaaag aacgccgcaa
     2341 tgtgatccat gatgtcgcca gcgggtgcct cgcgccgatc tgcctgcccg tcttgcaccg
     2401 aatgcataac gacgagtttg gcagatgatt tcgccaattg cggatagaac gcagcgtctg
     2461 gaaaaccgcg aatatcattg agataggcca caccacgcga caaggcatag gcttgcgtcg
     2521 cgggttgata actgtcgagc gagacgggaa tgccatctgc cttgagcgcg tccagcaccg
     2581 gcgcgatacg cgcgatttct gtgtcggacg aaacaggcgc ggcgtcggga ttgctggatg
     2641 ccggaccgag gtcgatcaca tctgccccct cggccatcag cttacgcgcc tgcgcaatgg
     2701 ctgcgtctgg cgccagatac cggcctccat cggagaaact gtccgaggtt atgttgacga
     2761 tgccgaaaat gatgagcgat ttattcatgg gggcttctat aataataata atcgagcatg
     2821 agtctcatac ggatgctcgg gtcgaaaggg aatccccagg cgagtaacct gtttgcggtg
     2881 atccgtttca tgatgatgta tctgtagcat tttggaaata aatatatcat ctctcaataa
     2941 gtagatatat ttatcttttt tgcttctttg gtgctgcaag tcaccaatac gagggaaacc
     3001 tgttacggag cggtttaacc gtaatcaaca gtgactggtg tcatgttcgg aagattaatt
     3061 gatagtaatc tacaaaggat cttcttgaga tccttttcaa tgaatcgtaa tctcttgctc
     3121 tgtaaacgaa aaaaccgcct ggggaggcgg tttgttcgaa ggttaagtca gttggggaac
     3181 tgcttaaccc ggtaacaggg ttcacgagac cgccattacc aaaactgttc tttcagtgta
     3241 gccgcagtca ggcctctact tcaagacctc ttaatctatc ttctcgtgca ctcctgttac
     3301 taatggctgc tgccagtggc gttttgtcgt gtctttccgg gttggactca agttgatagt
     3361 taccggacaa ggcgcagcgg tcggactgaa cggggggttc gtgcatacag tccagcttgg
     3421 agcgaactgc cttcacggaa ctgagtgtca ggcgtggaat gagaaaccgc ggccataaca
     3481 gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagctaccag
     3541 ggggaaacgc ctgggatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc
     3601 agattttgcg atgcttgtca ggggggcgga gcctatggaa aaacggcttc gccgtggcct
     3661 tatctcttct ttgctaagtg tcttcctggt atcttccagg aaatttccgt ccccgtccgt
     3721 aagccggtac cgctcgccgc agccgaacga ccgagcgtag cgagtcagtg agcgaggaag
     3781 cggaatatct cctgtatcgc atattctgct gacgcgccgg tgctgccttt tttctcctgc
     3841 cacatgaagc acttcactga ttttcgcaat cgtggcaaca tagtaagcca gtatacactc
     3901 cgctagcgct acatgactgg ttcagggctg cgccccgaaa cccgctaaca acctctcagc
     3961 tactggatat caccccatgc catctgaata tgatgaacaa ctggattcga ctgatacgca
     4021 gcttcccggc gaatatgacc tttttgattt aatggcgcag gttactgaag agaacctgca
     4081 tgcccctgtg gattttgggt cgcctgtcgg ccgggaagcg ttatagcttt ttcgcttgcc
     4141 cgcgtacgtg gtaatactcc ggtattacct tttatttctt tattttcatg taattacagt
     4201 ggatttttaa tacccgggta ttactcgtga aaacgaaata ttcctttttt ttcttttaat
     4261 tcatgcagtt gcgatgaaaa tcgcggctgc gttaggtgta tgaccatttt gaggttgaaa
     4321 aataatcaac catggcgggt gatttacgca ggttaagggg agatcgtgac aaaaaaagcg
     4381 ttcctcactg tatcgttcat gcctgcatga acgggttatt cttcctgact acagaattac
     4441 atcgctcctt cagttcatac atcactttta catcacttat acatcacttt tacatcattc
     4501 cttcagctca tacgtcactt tcatcctctg tgccatttgt acatcacccc cttcctgaac
     4561 gtgctgacag cgggttttcc ggctgcgccg gcaacgcttc acggccacgc aataaattgc
     4621 gtccccgttc accgttttcg gatgggaaga acgccggaaa caggacttac cccggctgaa
     4681 tggtgtgaat ttccgcgcta tgcacttgtg cgcatactca tgcatgccgt aaaaacagag
     4741 cctgcgcgtt tctggcgggt tttcgggtgg tttgttgcct gttttaccgg tttcccgtca
     4801 gaaacgccct gagggcctct caggcggtgc tgataacaga tgttatggta aatcagagtc
     4861 acctgcactg ttactctgaa ccggagcgaa acagccgcga ccagaatccc cgcttttttt
     4921 cctgtgccag cgcgtcagcc agacggctaa tttcggcctg tagttgttcc ctctcctcat
     4981 ttcgcaggcg gttttctgct tcccgcgcca tcttgtcttc aagcattagg gtaacagtct
     5041 gctgcagagc gtccagtttc tgctgaatga cgtctagctg agcattttcc gcctggctaa
     5101 cgacagattt ggagtgtccc tccttgtgcc tttttggtgt ctcagaagtg cgcagctcgc
     5161 cataggcccg aatcagttca ctggtctcga actcccggcg atcatttttt cctgtgcgat
     5221 agctgacaag gcctttagcc atgtcacgat ataattgcga gcgcgacttt cctgttaatt
     5281 tcagtgtctc ttgaactgtg tgccatgcca taaaagtgac agtgtctcct gtgtgtaccg
     5341 tattgtaaca gcagaacata ttactgtttt aacacaaaag gtctgaggct gcccgtgagg
     5401 cgtgaaaaat acacaaaaaa cctgttgacg gtgtggtttt gacgggcgta gtatttcgaa
     5461 tactgtatat aaacacattg ttataaatac agtttttgct gtttttaaaa gtcaaagagg
     5521 ataattttat gagtacagag ttaaatagcg gtactgtcgg tggttttggt cacatgggac
     5581 caaatgaccc ctacaatgat ggtcatagcg gctctggaag ttcgggaggt agttttaacg
     5641 gtggtggtaa tggtggccat gataatagta gtggttcatc ttatactgca tcctttggtc
     5701 tcatcactgg ccgacacccg ataactgtcc caggtacgaa gaatattaat tctgtcgatc
     5761 tttctgctgt cattaataac attatacatc atgaaggtgc acgcaataac atgtacctag
     5821 atactgcgaa caatgtaaga gtgggagtga cgggcactgg ctggcaatgt ctagcaacgg
     5881 caggcatttc ggctgagggt aaaagaactt tccgctaagc gatagactgt atgtaaacac
     5941 agtattgcaa ggacgcggaa catgcctcat gtggcggcca ggacggccag ccgggatcgg
     6001 gatactggtc gttaccagag ccaccgaccc gagcaaaccc ttctctatca gatcgttgac
     6061 gagtattacc cggcattcgc tgcgcttatg gcagagcagg gaaaggaatt gccgggctat
     6121 gtgcaacggg aatttgaaga atttctccaa tgcgggcggc tggagcatgg ctttctacgg
     6181 gttcgctgcg agtcttgcca
//
