LOCUS       NZ_CP079622             5251 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC16_AI1547MC
            plasmid pAI1547M_P5, complete sequence.
ACCESSION   NZ_CP079622
VERSION     NZ_CP079622.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20130505
            Assembly: GCF_019317065.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC16_AI1547MC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC16_AI1547MC
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 91.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:00:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,821
            CDSs (total)                      :: 5,694
            Genes (coding)                    :: 5,540
            CDSs (with protein)               :: 5,540
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 26 of 154
            Pseudo Genes (multiple problems)  :: 19 of 154
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079622.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC16_AI1547MC"
                     /isolation_source="Pus"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pAI1547M_P5"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2016"
                     /collected_by="Christian medical college"
     gene            join(4529..5251,1..633)
                     /locus_tag="KW551_RS28905"
                     /old_locus_tag="KW551_28880"
     CDS             join(4529..5251,1..633)
                     /locus_tag="KW551_RS28905"
                     /old_locus_tag="KW551_28880"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_014386473.1"
                     /translation="MFVYLTIENFRSVKEKSTLNLSASGSSNHLTTHIYKNDGMNVGTL
                     MSAGIYGANASGKSNVLMAFEAIKFIATESGDLKEGKRIPCYEPYALSSATNEAPVKFE
                     AEFYSVEGNRFSYEVAFIKNRILFESLDYYPSRVKANLFTRDESDTWETIKFGGHYKGG
                     IKKIPFFPNNSYLAKAGNNAASPDIIKEAYNFFRKGIRHIGLNEKIRISSFGEREDVVN
                     ETAKVLCMVDTGVSGISVREVEAEIPIKMDEEIPQEIKEIIEEDYKYRYMFSHPTDEGG
                     SVSFPMGRESEGTQKLFEIIPLIRSAFNDSMVVIVDELDNSLHPHIADLIVKLFNDPDV
                     NKKGSQLVFSTHNMQLMAPEKMRRDQIWFCEKNKGASSLYSLDDFDKKKIKTTTPYAAW
                     YDEGRFGGVPDINYLKVASFISGDISLVMPDIDVKELSDGFFEEFDGDLSDE"
                     /name="ATP-binding protein"
     gene            626..1246
                     /locus_tag="KW551_RS28910"
                     /old_locus_tag="KW551_28885"
     CDS             626..1246
                     /locus_tag="KW551_RS28910"
                     /old_locus_tag="KW551_28885"
                     /inference="COORDINATES: protein motif:HMM:NF025084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="WP_023312615.1"
                     /translation="MSKKHGNNKKVPQVMYIYCEGEKTEPLYLEAYISDRSKRTLSVFK
                     IPKTRKNTPEQLVDEAIKKKNSSSSAEGDVFWVVYDQEHLTPSSISCHQKAWNKANRHG
                     INIAISCVCFELWLLLHFGFTTKDFSSYDNLMSSSPLKKLITNYDKGSKLTYLAIRERI
                     GTAKDNARKLDIYAKTNYKNTHEPYLFPCYTSFHELLKAIDDF"
                     /name="RloB family protein"
     gene            complement(1399..1560)
                     /locus_tag="KW551_RS28915"
                     /old_locus_tag="KW551_28890"
     CDS             complement(1399..1560)
                     /locus_tag="KW551_RS28915"
                     /old_locus_tag="KW551_28890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023144844.1"
                     /translation="MHEPPVQSDRCAFSGNYHLESNPERHGSSPLAAAIGNGLEEVSLE
                     VLRQLRLN"
                     /name="hypothetical protein"
     gene            2197..2517
                     /locus_tag="KW551_RS28920"
                     /old_locus_tag="KW551_28895"
     CDS             2197..2517
                     /locus_tag="KW551_RS28920"
                     /old_locus_tag="KW551_28895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_014386475.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2726..>3157
                     /locus_tag="KW551_RS29345"
                     /pseudo=""
     CDS             2726..>3157
                     /locus_tag="KW551_RS29345"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3195..3680
                     /locus_tag="KW551_RS28930"
                     /old_locus_tag="KW551_28905"
     CDS             3195..3680
                     /locus_tag="KW551_RS28930"
                     /old_locus_tag="KW551_28905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_013631087.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            3684..3896
                     /locus_tag="KW551_RS28935"
                     /old_locus_tag="KW551_28910"
     CDS             3684..3896
                     /locus_tag="KW551_RS28935"
                     /old_locus_tag="KW551_28910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          1911..2083
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.659"
                     /coverage="100.0"
                     /evalue="9.74e-41"
     Typing          complement(1448..1964)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.462"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2508..4012
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.215"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaggttgagg cggaaatacc aataaaaatg gatgaggaaa tccctcaaga aataaaggag
       61 ataattgaag aagattataa atatcgttat atgttctctc atcctactga tgaagggggg
      121 agtgtttctt tccctatggg gagagagtct gaaggcaccc aaaaattatt tgaaatcata
      181 cctctaatta gatctgcgtt taatgatagt atggttgtaa ttgttgatga gttagataat
      241 agcttgcacc cgcatattgc tgacttgatt gttaagttat ttaatgatcc tgatgtcaat
      301 aaaaaaggct cacaacttgt attctcaacc cataatatgc agttaatggc tccagagaaa
      361 atgagaagag atcagatatg gttttgtgaa aaaaataaag gtgcgtcgtc tctttatagc
      421 ttagatgatt ttgataagaa aaagataaaa acaacaacac cttacgcagc ttggtatgat
      481 gagggtaggt ttggtggggt tcccgacata aactacttaa aggtagcctc atttatatct
      541 ggtgatatat ccttggtaat gccggatatt gacgtcaaag aattgtctga tggttttttt
      601 gaagagtttg acggggatct ttctgatgag taaaaaacat ggaaataata agaaagtccc
      661 acaagtaatg tatatatact gtgagggtga gaaaaccgaa ccattatacc ttgaggctta
      721 tatttcagac agatctaaaa gaactttaag tgtatttaaa atacctaaaa caaggaaaaa
      781 tacacctgag cagttggtgg atgaggcgat aaagaaaaag aattcatcat cttccgcaga
      841 aggtgacgtc ttttgggttg tttatgatca agagcatctg acgccttcgt ctatttcatg
      901 tcatcaaaag gcatggaata aagctaatag gcatgggata aatatagcaa tatcctgtgt
      961 ttgttttgaa ttatggttat tgttgcattt cggcttcaca accaaagatt tttcgtcgta
     1021 tgataatttg atgtctagct ccccgctaaa aaaattaata actaactatg ataaaggtag
     1081 taaactaact taccttgcta taagagagag gattggtact gcgaaagata acgcaagaaa
     1141 gctagatatt tacgctaaaa ctaattataa aaacacgcac gaaccttatc tatttccatg
     1201 ttatacatca ttccatgagc tacttaaggc gattgacgac ttttgaagta atccattgac
     1261 catggcttat tacaggctgt aatctatgaa aaataaaaac caccgctacc aacggtggtt
     1321 tttggaaggt acatgagcta ccaacttatg taccgggtaa ctggcttaga gggagcgcag
     1381 tcaccaaaaa ttgtcctttc agtttagcct taactggcgc agaacttcaa gactcacttc
     1441 ctctaatccg ttaccaatgg ctgctgccag tggcgatgaa ccgtgtcttt ccgggttgga
     1501 ctcaagatga tagttaccgg agaaggcgca gcggtcggac tgaacggggg gttcgtgcat
     1561 acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg gaatgagaca
     1621 aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca
     1681 cgagggagcc accaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1741 actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg
     1801 gcagcggcgc atcctttttc tgccccgttt gtcatgccat atgccttcgc tcttctccgt
     1861 gtgcaggtaa cgcccgccgc agccgaacgg caggagcggc agcgactgag tgagcgagga
     1921 ggcgtcatat cctctgaact tccattttct gccttacgcc cgcagctgca ttttcctctg
     1981 tcacatgaag cactttcctg attaccgcac tcatgccaac ataataagcc agtatacact
     2041 ccgctagcgc tacgtgactg gttcagggct gcgccccgaa acccgctaaa agcgactgac
     2101 gcgcctgcgg cttgtccaac accgtgccga ccgggaaaca tttcccgccc gtcccggtgt
     2161 tatcaggagg ctactctggc agacaaacgc aacaagatgc tcacgatgtg ggtgacggaa
     2221 gacgaacacc ggcggctgct cgagcggtgc gacggcaagc agctggcggc gtggatgcgt
     2281 cagacctgcc tcgatgagaa gccggcgcgt gccggcagac tgccgtcgat atcgccggcg
     2341 ctgctgcgcc agcttgccgg tatggggaac aacctgaacc agatcgcccg gaaagtgaat
     2401 gccggtggtg ccggtcatga ccgggtgcag attgtggccg cactgatggc aatcgacgcg
     2461 ggactggagc ggctgcggca tgcggtgctg gaaaaagggg gggatgatga tcgttaaatt
     2521 tcacccccgg gggcgcggtg gtggtgccgg tccggtggat tacctgctgg gaaaagaccg
     2581 gcagcgcgag ggggcgaccg tgctgcaggg aaaaccggag gaagtccggg agcttatcga
     2641 tgcctcgccg tatgcgaaaa agtacacctc cggcgtgctg tcgttcgcgg aggctgaact
     2701 gccgcccggg cagcgggagc agatcatggc cagctttgag cgggttctga tgcccgggct
     2761 cgataaagac cagtacagca ttctgtgggt ggagcacacc gacaaggggc ggctggagct
     2821 gaatttcctg atccccaaca ctgaactgct gaccgggaaa cgcctgcagc cgtactacga
     2881 tcgcgccgac cggccgcgca tcgatgcctg gcagaccgtg gtgaacggca ggctggggct
     2941 gcatgatcca aacgccccgg agaaccgacg gttactggtc acgccgtccg cactgccgga
     3001 aacgaagctg gaagccgctc aggcgattac acgtgggttg ctggccttag cctcatccgg
     3061 ggagctcaaa acgcgcgagg acgtcacagg ggcgctcaca gcggcaggtt ttgaggtggt
     3121 ccgcaccaca aaaaacagta tcagcattgc cgacccggac ggggggcgaa acatccgact
     3181 gaagggagcc atttatgagc agtcttttag cgctggcgaa ggacttggag cagcaatcga
     3241 aagcgcagca gcagagtacc ggcgagatgc tgaaagccgc attcagcgag cacgagcagt
     3301 ctgtaaaagc ggaactgagc gcaagcgcga agagaatcag cgacgccatc agcgcccacg
     3361 agcagggtat gaccgcggcg atgcagtcga accggctgag cgtgctgcgt atggtcggcc
     3421 ggacgtggct gaccatcacc ctggtctcgg tactgctgat cgccacgagc gggagcattc
     3481 tgtggtggca ggggcagcag atcaccggga attaccagac tatccgggcg caggagcgga
     3541 cgcaggccat gctgtcggag aagaaccacg gcgtgcagct gtcgctctgc ggggagaaga
     3601 aactgtcctg cgtcaaagtg aacccgaaag cgggagctta cggggaggaa gggaactgga
     3661 tggtactgga gaggaaatag cccatgacgg aactggaaaa acagttgctg accgcattag
     3721 agcagctaca tcaggactac tcgcaaaggc tggacgagtg ggagagcgcc ttcgcggaat
     3781 ggcagaagat gtctggtctt atgcaacggg agaacgcggc gctgaacgag cgcgtcacgc
     3841 gcttgagtca gcaggtggcg aacttgagca ggcagttaca gcgtttgagc cagtagtgca
     3901 gcggcatgaa atggcggtgg ccgccgaacg ggcatacgag cagaaacagc atgaaaaaga
     3961 gctggcggaa acccgttccc ggcagcggag ctatgacggt ccgacgctgg gctgactgcg
     4021 cgcatccagt gacaaacccc aagaagaagg gctcataaat ttcccgctaa atacggtcgt
     4081 gatgtttcca tcacagcgta tacaacggag ggattatgag actgtttaac gtgattgaga
     4141 cggtgctgaa ttgcctgtac atggcgctgg agatctggga ctggctggag aagcattttc
     4201 ctgtttttaa ggcgttcatc cggattctgg cctgaaatca gaccgtatgc atctggctgc
     4261 atgaccatgc agccgggtaa aatttatctg tcacgaacgg ggcggtgtat cgggtggttt
     4321 gttgccggtt ttacctgact gccgccccgt ttgtggcgaa cctgtccgga gcggtgcggg
     4381 caacagatgt tatggtaaat agcgtgtaat tataaatagc acaacgtttt ttaaggaaga
     4441 tgttgttcca aaattaaaac atataggata tctttcctat gattgatttt tcgctttttt
     4501 ggcgaaaaat ccccgttagg aggtatgcat gtttgtttat ttgactattg aaaattttag
     4561 gtcggttaag gaaaaatcga cattaaactt gagcgcatcc ggttctagta accatttaac
     4621 aactcatatc tataaaaatg atggaatgaa tgttggaacg ctcatgagtg ctgggattta
     4681 tggtgctaat gcgtctggta aatctaacgt tctcatggct tttgaagcta taaaatttat
     4741 cgcaacagaa agcggtgatt tgaaagaagg taaacggatt ccctgctatg agccatatgc
     4801 tctttcttcg gctacgaatg aggctcctgt aaagtttgag gctgaatttt attctgttga
     4861 ggggaatcgg ttcagttatg aagttgcttt tattaaaaat cggattttat ttgaatcatt
     4921 agactattat ccatctaggg ttaaagcaaa tttatttact agagatgaga gcgacacctg
     4981 ggaaacaatc aagtttggtg ggcattataa aggaggtatt aaaaaaatac cattcttccc
     5041 taataattca tacttagcga aagcaggtaa taatgccgca tcaccagaca taattaaaga
     5101 agcttataac ttttttagaa agggaattag acatataggt ttaaatgaaa agataagaat
     5161 atcttctttt ggggagaggg aggacgtcgt aaatgaaacg gccaaggttt tatgcatggt
     5221 tgacactggt gtttccggta tatctgtacg g
//
