LOCUS       NZ_CP079614             3991 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC15_AI1547PC
            plasmid pAI1547P_P8, complete sequence.
ACCESSION   NZ_CP079614
VERSION     NZ_CP079614.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20130504
            Assembly: GCF_019317025.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC15_AI1547PC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC15_AI1547PC
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 91.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:02:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,494
            CDSs (total)                      :: 5,370
            Genes (coding)                    :: 5,219
            CDSs (with protein)               :: 5,219
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 85 of 151
            Pseudo Genes (internal stop)      :: 22 of 151
            Pseudo Genes (multiple problems)  :: 20 of 151
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079614.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC15_AI1547PC"
                     /isolation_source="Pus"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pAI1547P_P8"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2016"
                     /collected_by="Christian medical college"
     gene            join(3572..3991,1..3)
                     /locus_tag="KW550_RS27345"
                     /old_locus_tag="KW550_27320"
     CDS             join(3572..3991,1..3)
                     /locus_tag="KW550_RS27345"
                     /old_locus_tag="KW550_27320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_040214596.1"
                     /translation="MAIVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            807..1079
                     /locus_tag="KW550_RS27350"
                     /old_locus_tag="KW550_27325"
     CDS             807..1079
                     /locus_tag="KW550_RS27350"
                     /old_locus_tag="KW550_27325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004897311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_019405140.1"
                     /translation="MATDAQVKEINALIKKYPDSCSICHEVYDEDDVTYTVFGYDRKGK
                     IQVTTGCCAGMLTEPVLLGVCGCFDPEERDEIMQNHPMAKQFFTE"
                     /name="hypothetical protein"
     gene            1660..2634
                     /locus_tag="KW550_RS27355"
                     /old_locus_tag="KW550_27330"
     CDS             1660..2634
                     /locus_tag="KW550_RS27355"
                     /old_locus_tag="KW550_27330"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_114269875.1"
                     /translation="MAFFNLLFVASLICFGGVMGSYSRGCRDSQNKFSKDKDENNKRAS
                     VYRFGIASAFICVPFISSFLHVDYSSIIFPVADGGGTKFIEQILLLISVSGISAYLGYA
                     LLDGLANKVLKEQVDGIDKKQQDLEAEQDEFKDELDRSKELIEQLEMDKKTTKFELGYF
                     KAISAVDKAESMMSIPDEALSVKKKLTEALDAVTESLSLVKREDVAKDDYDKLLVLKAY
                     ILKRLDRIDDALSITDELLMSNEDNPILIYNKACYQYILRRCQADNSDIKDMIRRALTI
                     KVTDPEFIRRQEKIRTKVIGNKDNDLEGLFTDAELEELKVAIK"
                     /name="hypothetical protein"
     gene            complement(2861..2973)
                     /locus_tag="KW550_RS27585"
                     /pseudo=""
     CDS             complement(2861..2973)
                     /locus_tag="KW550_RS27585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            3381..3572
                     /locus_tag="KW550_RS27360"
                     /old_locus_tag="KW550_27335"
     CDS             3381..3572
                     /locus_tag="KW550_RS27360"
                     /old_locus_tag="KW550_27335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_040198051.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLDKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(2862..3333)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.777"
                     /coverage="92.0"
                     /evalue="7.8799999999999974e-146"
ORIGIN
        1 tgatcccgcc atgcttccat tttgctctcc ggccattccg gggagttcct gaaccttccc
       61 aaaaatcgtc agatccctga acgctcctca ccactaaaaa tacccacaca ctacgcaaaa
      121 ttaaaatttt gcagatagac ctttattttc aatcagttat ctattttaaa ttacgcaatt
      181 ttattttttg taaggtgaat ccgagtcgtg gacgactcta tggcgaatcg ccgatgactt
      241 gcccacgatt catatgtgaa tcacgccata taatcagtac gttatgatga cttgactcta
      301 gagtcgactt gccgggttgg tggctttttg accatggcga ctctagagtc aagtcacgag
      361 tcgaatcgcc ggtgaatcgt gtggtgagtc gtccggcgac ttgccataat gtgattgctg
      421 gtctcgacaa acccgattcc tgaacgtgct gaaaaccggt tttccggctg cgccggcaac
      481 gcttcacggc cacgcaataa attgcgcacc cgttcaccgt tttcagtctg gtttggtgcg
      541 ggttttgagt tgcctggcct gaattcaggc tgaaatcccc agacagtcag tcaagtgcat
      601 aacgattcat gcccgtaaaa cgccctggag cgattttgag gcgttttcag ggggtaagac
      661 gtaggattta atgggttacg ccccagaatc gttctgaggc cgttttagcg gtgcgtgtaa
      721 tgggacgtta tggtaaatca acggcggatg cctgagtctg tgttagagtc agcggattgt
      781 gataatcagt gactcaggga gatgttttgg ctactgacgc tcaggtaaaa gaaattaatg
      841 cactcatcaa aaaatacccg gactcatgct ccatctgtca tgaagtctat gatgaggatg
      901 atgtgaccta caccgttttt gggtatgacc ggaaaggtaa aatacaggtc accacgggat
      961 gctgtgccgg aatgctgaca gagccggttc tgctgggtgt ctgtgggtgc tttgacccgg
     1021 aagaacgtga cgagatcatg cagaatcacc ccatggcaaa gcagtttttc acagagtgat
     1081 tccgtatctg ttgagtctga agcccgcctt gtgcgggctt tttcatatct gcgtgaaatg
     1141 aggcgttatg gtaaatgcac gcattcattc ttttgaaaaa tccagaatga cgaatacatg
     1201 gggcatgttt atatgactat tgagaggaag gatgttattg taaaaaaatc ctttaaattc
     1261 aatcatttaa ttatggtagc caaaggggcg gggcttcgct tattcctaag gaataagcga
     1321 agccccgccc ctttggctac cagtgcatta tacttttttt ttcacccaat gtcaatacat
     1381 gggttgattt tatcatttta agcatttttg ttgttgtgag tgctgggttt atgccctata
     1441 ttcagcgcta agcaagcatg ggcttgcaaa ggaacaatta atgagaggtg ctctatgaaa
     1501 aaaatcaaac ttcacgatga gcgtccagac ttgtaagtgt caagtcagca gaaaaaggca
     1561 gccaaggctg ccttttttgt tattggttag tatatgattg agcttatctt aacttacctg
     1621 aacaaagttt tattgtttgc tttgcgcaag gattctctca tggctttttt taacctattg
     1681 tttgttgcat ctctaatttg ttttggtggt gttatgggga gctattccag aggttgccga
     1741 gactcccaaa ataaattttc aaaagataag gatgagaata ataaaagagc tagtgtttat
     1801 agatttggta tagcatccgc atttatttgt gttcctttca tatcatcatt tcttcatgtt
     1861 gattactcaa gtataatatt tcctgtggct gatggtggtg gaactaagtt catagaacaa
     1921 atattgcttc ttatttctgt ttcggggatc tctgcgtatc ttgggtacgc attactggat
     1981 ggtcttgcca acaaagtatt aaaagagcaa gtagacggta tagataaaaa gcaacaagat
     2041 ttggaggctg aacaagatga gtttaaagat gagcttgata ggagcaagga gctgatagaa
     2101 cagcttgaaa tggataagaa aacaacaaaa ttcgagctag gatactttaa agccatttca
     2161 gctgtggata aagcagaatc aatgatgtcc attccggatg aggctctttc tgttaagaaa
     2221 aaactaactg aggcattaga tgctgtaacg gaatcattgt ctcttgttaa aagagaggat
     2281 gtagctaagg atgactacga caagctctta gtgcttaaag cttatatttt gaaaagactt
     2341 gatcgtattg atgatgctct gtcaattacg gatgagttac ttatgagtaa tgaggataac
     2401 cctatactta tttacaataa ggcttgctat caatatattt tgcgtcgttg ccaagctgat
     2461 aactccgata ttaaggatat gattcgcagg gctctgacta ttaaagttac agatcctgaa
     2521 ttcattcgca ggcaagaaaa aataagaacc aaggtcatag gaaataaaga taatgatctt
     2581 gaagggttgt ttactgacgc tgagctggag gaattaaagg tggctatcaa ataggcagtg
     2641 gctagctcta tgaattccaa aaagatcctt gacgatcctt tgaggtgcag cgtactctac
     2701 ccctcaaacg aaaaaaccac ccggcaaggt ggtttttcga aggttcagta accggcaagt
     2761 ttctgaaccg aggtaactgg cttagaggag cgcagtcacc aaaacagttc gtttagttta
     2821 gccttaactg gcgcataact tcaagactaa ctcctctaaa tcaattacca gtggctgctg
     2881 ccaatggcga taagtcgtgt ctttccgggt tggactcaag agatagttac tggaacgggc
     2941 gcagcagtcg gactgaacgg ggggttcgtg catacagtcc agcttggagc gaactgcctt
     3001 cccggagccg agtatcaggc atggaatgag aaaacgcggc cataacagcg gaatgacacc
     3061 ggtaaaccga aaggcaggaa caggagagcg cacgagggag ccaccagggg gaaacgcctg
     3121 gtatctttaa gtcctgtcgg gtttcgccac cactgatttg agcgtccgtt tccgcgatgc
     3181 ttgtcagggg ggcggagcct atggaaaaac agccgttcgg cggcctttgc ttttctgcct
     3241 catatcttgg ttgcctacgt ttttatgtac acgctcctgc cgctcgccgc agccgaacgg
     3301 ctggagcgta gcgactgagt gagcgaggaa gcggaaaaga gactagtttg acattgagca
     3361 ctgacgctct gaggcttctt atgacaaaac aacagaaaac agcgctgaac atggctaaat
     3421 tcatccagaa tcagtctctg ctcctgctgg ataagcttaa cgagcttgat ctcgatgtcg
     3481 aggccgacct ttgtgagaaa ctccatgatg atgcggagca tcttttccgt acactgtcct
     3541 cgagactgga tgaattacag gatggtaact gatggccata gtgtctatta gtgaagcggc
     3601 acggcttgtt aaggttagtc gccccacaat ttacaagatg attaacagtg ggaagttaag
     3661 ttacacctct gtggtaaagc acgggaaagc tattaaagtt attgatactt cagagctaat
     3721 tagagtgttt ggttctcttg atggtgtaat cgatactgta aaatatgatg taaaaagtga
     3781 cgcagaatct acaggtatta acagcgttgg tttacatgat ttacaacata gaatcgcttt
     3841 acttgaagct gaaaacgatg gtcttaaagg ggctgttaag gctcgagatg agcatattga
     3901 ttcccttcgt caggctatgc agcttctgga gcacaagcac gaaccttctt caccacccca
     3961 ttccccgtgg tggaaattct ggaaaaaatc c
//
