LOCUS       NZ_CP079602             9730 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC10_CMC309MC
            plasmid pCMC309M_P3, complete sequence.
ACCESSION   NZ_CP079602
VERSION     NZ_CP079602.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20115188
            Assembly: GCF_019317005.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC10_CMC309MC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC10_CMC309MC
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 70.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:04:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,423
            CDSs (total)                      :: 5,303
            Genes (coding)                    :: 5,152
            CDSs (with protein)               :: 5,152
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 67 of 151
            Pseudo Genes (incomplete)         :: 82 of 151
            Pseudo Genes (internal stop)      :: 30 of 151
            Pseudo Genes (multiple problems)  :: 23 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079602.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC10_CMC309MC"
                     /isolation_source="Endotracheal aspirate"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pCMC309M_P3"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2018"
                     /collected_by="Christian medical college"
     gene            join(9249..9730,1..1480)
                     /locus_tag="KWY78_RS26840"
                     /old_locus_tag="KWY78_26825"
     CDS             join(9249..9730,1..1480)
                     /locus_tag="KWY78_RS26840"
                     /old_locus_tag="KWY78_26825"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_063938523.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            1480..2211
                     /gene="mobC"
                     /locus_tag="KWY78_RS26845"
                     /old_locus_tag="KWY78_26830"
     CDS             1480..2211
                     /gene="mobC"
                     /locus_tag="KWY78_RS26845"
                     /old_locus_tag="KWY78_26830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071653262.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            2218..2748
                     /locus_tag="KWY78_RS26850"
                     /old_locus_tag="KWY78_26835"
     CDS             2218..2748
                     /locus_tag="KWY78_RS26850"
                     /old_locus_tag="KWY78_26835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938511.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2776..2955)
                     /locus_tag="KWY78_RS26855"
                     /old_locus_tag="KWY78_26840"
     CDS             complement(2776..2955)
                     /locus_tag="KWY78_RS26855"
                     /old_locus_tag="KWY78_26840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3024..3401
                     /locus_tag="KWY78_RS26860"
                     /old_locus_tag="KWY78_26845"
     CDS             3024..3401
                     /locus_tag="KWY78_RS26860"
                     /old_locus_tag="KWY78_26845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938513.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="hypothetical protein"
     gene            complement(3463..3762)
                     /locus_tag="KWY78_RS26865"
                     /old_locus_tag="KWY78_26850"
     CDS             complement(3463..3762)
                     /locus_tag="KWY78_RS26865"
                     /old_locus_tag="KWY78_26850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_063938515.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3752..4015)
                     /locus_tag="KWY78_RS26870"
                     /old_locus_tag="KWY78_26855"
     CDS             complement(3752..4015)
                     /locus_tag="KWY78_RS26870"
                     /old_locus_tag="KWY78_26855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938517.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            4392..5477
                     /locus_tag="KWY78_RS26875"
                     /old_locus_tag="KWY78_26860"
     CDS             4392..5477
                     /locus_tag="KWY78_RS26875"
                     /old_locus_tag="KWY78_26860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WP_063938519.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     gene            5474..7663
                     /locus_tag="KWY78_RS26880"
                     /old_locus_tag="KWY78_26865"
     CDS             5474..7663
                     /locus_tag="KWY78_RS26880"
                     /old_locus_tag="KWY78_26865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WP_063938521.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     Typing          complement(7869..8449)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.625"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1481..2208
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.885"
                     /coverage="100.0"
                     /evalue="3.84e-144"
ORIGIN
        1 gcttgtcgcc ggcatccttc tggcgcggct gctggcattt ctgctgcttg acggtcattt
       61 tgcggccgct gacgaggcgg tgaatgcgaa actgaaccag gagagccggc gcagcagcca
      121 gcggaccgga gaaatgaccg acgtccgcca cctgcatttc ggggagccgg taccggttaa
      181 cgcgctggcc gatttttcca cagagcaggc ccggaaacag caggcggtgt ttctgggtaa
      241 ggatgagcag ggccagccgg tgctggtccc gcgtgatacc tggcgcaaga ccaatattca
      301 gatcctcggc ctgcccggga gcggcaagag cgtgatgggc accaacgcgc tcattcgctg
      361 cgtacgcgat ttcggggacg cggtggtgta cttcgacccc aaaggggacg cgtgggcgcc
      421 gcacgtcttc cgggcgcact gcccggactt caccctgctg gatctgcggc cggggaaacc
      481 ggcgcagctg aatctgtttc gcgatctcga ccagtacgcc ctgaagaatc tgctggttgc
      541 cgggtttaac ctgagtgaga ccggcgacgt ggcggaccac taccgcatca gcgagcagaa
      601 agcggcgaag ctgattgcgg agcagttccc gcagggggca aacattcagc aggtgctggc
      661 ggcggcatac gcgctgccgg aggccctgaa aaaggacgtg aaggggctga tcaccaagct
      721 ggagaacgtg gccgacctga gcgtcctgca gacggacagc ggcatcgacg tggcggggat
      781 aatcaacggc ggcggctgcc tgtacgtcat cggctcgatg gacgatgagg cggtgatccg
      841 ggtgcagaag atgctgtttg cccgctgcgc gcaaatcatc attgcgcggg atgagttccg
      901 gaggtggccg cacgcgagca tcatgctcga tgagattaaa tacctgctgt cgaagtacgt
      961 gctgaacgcg ctggggacgc tgcgcagtcg ggactgcaac ctgctgctcg cgcaccagtc
     1021 gctgggcgac ttcgggcagt gcggccagga tctgcccgct gagtttgtga aaaccacggt
     1081 gctggataac acgccgatcc gctggttcta ccgggcggcc agccaggagt ccgcgcagtg
     1141 ggcggcgggc cagaccgggg aaatccgggt ggacgtggag cgccgccggg ccagccggga
     1201 ggcggggaac gtggagcata tcagcgggga cagcttcatt cagaaggagg cgcggccgct
     1261 gtttgacgtg aacaccctgc agcacctgcc tgacgggttt gcggtgatga ccgggctggg
     1321 cgtggcccgg atggcgttca gcagcccgct gcgggttgaa cgccgggaaa tcccgctgca
     1381 gcgcttcccg gtgctggcga aaacggaccc gctggcggag taccagccgg aggccgggcg
     1441 gcagcggccg ggtgatgatg attttgcggg gctgtactga tggcactgga gcgatacaac
     1501 gtcagccacg cgaagcggca ggcgcggaac gcggagaaaa cccggctgac cctgcgctgg
     1561 ctgcgggaag agctgtgttc cacggcggag ctggttgcgc gacggctggg gattgcggcg
     1621 gtgcagccgg tttaccgctt cctggacagc ctggtggcga aagggctgct ggtccgggcg
     1681 aaatacccgg ttgacggccg tcaggtcagc gtgtggggac tgacgccgca cggggtggcg
     1741 ttctcgttcg acgaggacga accgctgacg gacgttattc cgtttcagcc gtcaagggtg
     1801 tcggcggcgc agctgccgca ccggctggcc gtgcagtccc tgcgtctcgc catggaggcc
     1861 cggggggcct ccggctggcg gtacctgcac cgtatggccc tgaaggggat gaaggtgccg
     1921 gacgcgctgg cggagctgga cggcagaacg gtggcgtttg aggtggagcg caccgtgaag
     1981 agccgcaggc gctatcagga ggtggtgtcc gggtatctgt tcaatcgcaa agcgaacggc
     2041 atcgatgaaa tctggtacat ctgcccggac cgggcgacgc aggtcagggt gcagcgggcg
     2101 atcctgtcgg tggatgaaat cgtgaatcca cagaccggcg aggcccgcaa aacggcggag
     2161 ctggaccggg agcggctgtt tgcgtgcttt aaatttatga caacggagtg agggattatg
     2221 gcgctgtaca tggtatttat tgaagacgcg gaggaccggg acggcgagcg ctgcatcatg
     2281 ccgaccagcg agcatcttga agatatggtg gtgctcgcct ggcagaccgg ggcgtatgtt
     2341 ttctgttttg tcgaccgggc ccagcatctg gcgatgatga aatccatcgg gatgtatgac
     2401 gagcggctca acagcgacga aaaagccacg tactttttca ccggtaaaaa tccgcaatgg
     2461 gaacgggaca aacccgcagg aatgaagctg tgtgattacc tctatcgcaa ggtggagcgg
     2521 ttcgccatcg agaacggtgt gcaggtggtt gatgccggga agtgctcacc ggagcagatt
     2581 agcgcgatga ttgaggtggt cagcgagcgg cacggggaaa gcgcggtgaa cgagaagctg
     2641 aaagcggagc tgatgcggga atataacagc cgctatttta acgtgccggt gcctgaactg
     2701 ccggaggaca aacccgcccg ccggtggtgg ccgttcggca aaaaataaac ggcagcaccg
     2761 gccatgccgg tgctgttatt ccttcccgag cttatcccgc atcacctgcc agagtgtttc
     2821 cgcatcctcg tgcagccgct cgcacatgtc ggccggggta tccatatcca gctcgttcag
     2881 tttctcgagt aacagcagcg actggtcctt aataaattta gccatattga gggcagtttt
     2941 ttcctgctta gtcatgtttt ctgtccttcc gggtgtggtg tgcttcctgt cgggtatact
     3001 gtctcaaaac cggaggtgag acaatggcgt gggtgacggt gagacaggcg acggaactga
     3061 cggggaaagc ccggagttcc ctgtataggg atatggcgaa aggccgcgta tcataccgta
     3121 ctgaagctga tggtggtcgg gtggtggata ccagcgaact tatccgggtg tacggtgaat
     3181 taagacagat tgagactcac gagcgggacg ggttgagact gtcggatgag actgaaaaaa
     3241 tggcctccga cgcgttgatc gcggagataa aagcactcag ggaggaggtg gccggactgc
     3301 ggcaggaaat gcaggccatg agactgctgg aacacaaacc agagacagca gagacaccgg
     3361 ccggaacatc gcgccgatgg tggcagtggg gaaaacgctg acgcgctgtg cttgtccaac
     3421 cggcgacggc cacgcaatga attgcgcccc catcaccggt tatcagcgaa cggtatgggg
     3481 attctggcgc tggccaacca cggccagaac ccggactttc ctggtctggt catcaacatc
     3541 atagaggaca tgctgtccca tcagactgat ccggcgaacg tcctcgccat agcgtgagtc
     3601 cacttcatat aggcgcggga agtcggccag tttgtccgct gcagcgagaa ctcgatcggt
     3661 aacgttctgc gcatcccaca gggatgaatt ttccgcaatg tagtcaaaca atgctgtcac
     3721 atcctcgatg gctccctgct tccattccac ctcatacggc atcgcgtagt tcctgatagc
     3781 ggccatgcag tctctggcgg agttgttccg tggtcatgcc cgggcgcgtg tcagcgcggg
     3841 cggcggtcac tttagcgcga acctgatctt catattctgc ctgggccagt tcttcgttag
     3901 ccaggcgctg caacagtgca gctttgtccg ggccaccttc aggcagtatg cggggcagtc
     3961 tctcggccag ttcccgcagg aaattggcgt tttcatagtg tgtactgttg ctcattgtgg
     4021 agcctcccgg gctgatacgt cgatatgaag gagtttagca gggggcggtc tgaaagaata
     4081 tgctcgtgtc agtcaggctg atgaagacga aagctggtaa aaaagggctt acgacaccca
     4141 ttttacgtca aactgcacga catgcagttg cgtgcatagc catgcagcgg gttaaaattt
     4201 atcaggcgcg tttccggcgg ttttccgggt ggtttgttgc cggttttatc ccgtagccgc
     4261 cggaaatgcc ctcagaccgt ctgagcggtg cgcgtaatgc gacgttatgg taaatcatgt
     4321 gattaatggt agtgataatc attatcactt ttgctatatt cttgtttctt ttattgtaag
     4381 gaaatgtaag tatggcaatc acgattaaat ccgccgttaa tgtccgtagc agagtattaa
     4441 tatactcttt actactgttt actggttcag cgtatgcggc tgatatagct gaatcaacat
     4501 tcctgactcg tgatatcggc aatggtctgt atgaaatgtc ttttgatggc aaaaataata
     4561 gtctctgggc agcatcatcg tcttcattcg acagagataa gggccacgga attgtttatc
     4621 agttagaacc atccactctg aaaacaaaat ctgccgttaa aatcgcacgt cttgggtttg
     4681 ccacagcact ggatgaggaa catgagcgac tttatatcgg caatactctg gacggctcag
     4741 tgacggtcat tgatactcta accggaaaag aactttcagt aattccgtta actgagggac
     4801 aggatgaggc tcatttcaga ccgaccagag aaatggtcat tgacaaaaaa aatcagcgtc
     4861 tttatgtctc cagcgccgca gataaagggg ctctgtgggt tattgacatg gctgtcggga
     4921 agaaaattaa aaccattgat ggactggggc acgctgttac tggaattgcg ttaaataaaa
     4981 atggggataa tatttatctg gttaacgggg atggtgagat tattaacctg gatgccagta
     5041 gctatgaaat aaaaaaacgg tttgttgtag agcctgagaa aaaacacttt ttccttaatg
     5101 taagtctgga tgaaaaaaac ggacgtgcat ttattaccga cccggatatt cccggggtgc
     5161 tggttgttga tgtaaactcg ggaaaaatac tacacagaat tgatgttata aattcactgg
     5221 cgattttatt taatccttta cggaacgaaa tctatatcac gcaccgtaat gcaaaacagg
     5281 tcagcattgt ggacagtaat tcttattacg taaaaacaac gataaaaacc cgcctgatgc
     5341 caaacagtct gtctttatca catgacggtg aaaatctgta cgttagtgta aaacaagaaa
     5401 aaaaagagat gagtactatg aaggattaca tactcaaaat cgaattatct aaaattgcta
     5461 tggagccaga agaatgaata aatatttagg gctgagcata gtatatatga tgtatacatc
     5521 atctgttatg gctgatgatt ctggcgcagt tagtaaagat aaaaatattt caccagaaga
     5581 aacattaatt gttcatggga ccgctgtttc tgaaagcaca acagaaggaa cagattcata
     5641 cacaacccag tcagccaata cagcatcaaa gatgaatctg tcattccggg atacacctca
     5701 gtcaactaca gtcattacgc gacgaagaat ggatgatgta gcagcaaaaa atattattga
     5761 tgtggttaaa accgctcctg gattatatgt cagtacagca gaaggttccg gaagaccgaa
     5821 ttttatgtcc agaggattta atgctaatgt catgtatgaa ggatttagcc tgccgtggaa
     5881 ttcatacatt ccaagttcac aaactagttc agctattttt gaccgggtgg agatagtccg
     5941 cggggcaaca ggtcttgttc agggtgccgg agacccttca gccgcgataa atatgataca
     6001 caagcgtccg acccggcaat atcaggccag cactaacatc ggtgccggac ggtggaatga
     6061 ctaccggggt ggggttgatg tatcaggacc actgaatgac agcgcgacct tacgcggaag
     6121 ggcagttgtg gatggcgaaa acagtgagac cttcagggat agtgagaaac gcaggaataa
     6181 cgtcatttac ggaattatgg aggcagatct gactgataat accctactta cgattggggg
     6241 atatcagcag tatgaatata acaatcattg gtggtatgac ttacctattt ctggtaccgg
     6301 acaccacctt aatcttcctc gctcgacgtt tacaggcaat gactgggagt ttgaaagaag
     6361 taaaagtaaa actgcatttg caactcttga gcagaagttc agtaatagct ggaagctgaa
     6421 attttcaacg ctgcaaaact ggagaaatgc tgacctgtta gggacggcaa cataccggca
     6481 taaagatacc cgacaaagta tgtatcagca agtatgggga tcactgaggg aatataattc
     6541 caaaagctat gacctgtcgc ttaatgggga ctatgcttta tttggtcgtg ttcatcaatt
     6601 cgtggctgga acaactcagc aaaaatacac cgataaagat acaggttata catattcccc
     6661 aatatatatt gagaatgtta atatatttga tcatggttca cattcttcga aaaaacccat
     6721 gaagatgtca ggtgatgcat cctacgacgt cactgaacaa aaaagcgcct acgcagcaac
     6781 tcattttcta cttagtgata actggaaaat attgctgggt ggccgtcttg actggtatga
     6841 atatacaagt aaaggtacat ccattaatac aggttataaa gttaccagaa atatcaccaa
     6901 atatgcaggt tcaacataca atcttgacga tcatcatacc atttatggga gctataccga
     6961 tatattcaat ccacagtcaa gcaccgacct caatggtaaa gtgatcaagc ctattgttgg
     7021 taagaattat gagacaggta ttaagggtga gtattttaac ggcttgctta atgcctctct
     7081 ggcggtattt caggtagacc aggaaaatcg agccaggctg cttcccgatc agacagggtg
     7141 tgttactaat ggcaatcctc aatgctatga ggctagcggt aaagtacgca caaaagggat
     7201 agatttcgaa cttcagggca tggttacgcc actctgggaa attggggtgg gatatacata
     7261 tgcagatacg cgatatataa aagatggaga tcgtagtaat gaaggcaagc gattcaatac
     7321 tgccgtgtcg ccacaaaatt tatttaagat ttcgagcatg tacacctttg acggaaaatt
     7381 aacaaactgg cgagcaggag taaacacgta ctggcaaagt aaaatatatg ctaacataac
     7441 agacacaagg gatggcaatc cggttattaa taaacaaaag gcttattcaa caactgacat
     7501 tattattggc tatactccaa tgccacaact ggatttacag ctcaatataa ctaatgtctt
     7561 tgataaggtt tattatacat ccataggtga atcgattgga tatggcacgg aaacatatgg
     7621 agaaccaaga aatgttaaaa ttagtgcaac ctatagattc taaccatgcg gattgggtct
     7681 tctcctcgga tttgccccta taaatccgtc ttttgtggtt ccggcgtaca accccctgtt
     7741 tgccacttca gttgatggtt aatcaaatca tgtagaggat cttcttgaga tcctccatgg
     7801 ttcgtcgtaa tcttttgccc tgtaaacgaa aaaaccacct ggggaggtgg tttgatcgaa
     7861 ggttaagtca gttggggaac tgcttaaccg tggtaactgg ctttcgcaga gcacagcaac
     7921 caaatctgtc cttccagtgt agccggactt tggcgcacac ttcaagagca accgcgtgtt
     7981 tagctaaaca aatcctctgc gaactcccag ttaccaatgg ctgctgccag tggcgttttt
     8041 gcgtgtcttt ccgggttgga ctcaagtgaa cagttaccgg ataaggcgca gcagtcgggc
     8101 tgaacgggga gttcttgctt acagcccagc ttggagcgaa cgacctacac cgagccgaga
     8161 taccagtgtg tgagctatga gaaagcgcca cacttcccgt aagggagaaa ggcggaacag
     8221 gtatccggta aacggcaggg tcggaacagg agagcgcaag agggagcgac ccgccggaaa
     8281 cggtggggat ctttaagtcc tgtcgggttt cgcccgtact gtcagattca tggttgagcc
     8341 tcacggctcc cacagatgca ccgaaaaagc gtctgtttat gtgaattctg gcaggagggc
     8401 gaagcctatg gaaaaacgcc accggcgcgg ccctgccgtt ttgcctcaca tgttagtccc
     8461 ctgcttatcc acggaatctg tgggtaactt tgcgagagtt agcagcgccc gccgcagtct
     8521 cacggccgga gcgtagcgac tgagtgaggg aggaggcgca atatcccccc tcccgcagac
     8581 ggccaccgga cgcgcggatg tgacatattg caccgtgtta ccgcctttcc cttccctgcc
     8641 gataaatcat tcagataggt gaagtctgtc ggttttctgc tgaatatttt acgttaacct
     8701 atctctgttg cttttttctc cttgcggaga aatgcaggtc atccccgctg gggcgtggct
     8761 gacttcagga ggaaatcccc tgcttacgct ggctggacgc caccgacgca cggtctttcc
     8821 ccctctttct ggctcagctc ggctggacgc cggccacgcc agaaattcac ccggcccccg
     8881 tcaggctcac gcctgaccac ccacaaaaac ccacggtgct gaacgcacct gacgaagcag
     8941 caaagctgct cctgattttg cgggtggtca gccgctcgct atggcgaact ctgacggggg
     9001 ccggaacacc ggaaaagacc gcactggggt gccgcctttt cctctcacac gctggggcgt
     9061 gctcgttgtt cctccggggc tgcgcctttt ccgcttgggg cggaaagagg ctggcctccg
     9121 tggtgaaagc tgaatgcaat caccacggac gtgaccgccg ccgccatgcc tcggggcatg
     9181 tccgcggctt gtgcagcagg ggctgcacac cacgccggta aacccgtcgt ctgaaggaga
     9241 gagattgcat gtttaatacg gattcgcttg cctggcagtg gcatatcccg gagctggccc
     9301 ggcagattta cccgtgggtc agctggctgt acagggagta ttacggtctg gccggggctt
     9361 tcctggttat cggcctgttt ttcggtctgg ctgaactctg ggccggacgg atgcgttttg
     9421 tggggctgag tcataaggag cgacgggagt atgccgatcg ctataagcgc gataataacc
     9481 ggactgcgcc attttcggtg ctggcttcgc tggattcgcc cgggcggatg gtcagggcac
     9541 ggcggcatgt ctggaactac gcgtactggc tgaccggcgc tgccggtctg gcccttctgg
     9601 gggggctggt tgtcgccgtg tttatcgaga gctggggaga aaccgtcctg cgtccggacc
     9661 tctacgcgaa cgcagattat tacctgctgc agccggctaa cctgttcggg attgtctggt
     9721 gtgccggagg
//
