LOCUS       NZ_CP079182             7819 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC13_AI2040PC
            plasmid pAI2040P_P3, complete sequence.
ACCESSION   NZ_CP079182
VERSION     NZ_CP079182.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20115569
            Assembly: GCF_019287095.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC13_AI2040PC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7819)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC13_AI2040PC
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 85.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:03:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,583
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,297
            CDSs (with protein)               :: 5,297
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 69 of 159
            Pseudo Genes (incomplete)         :: 93 of 159
            Pseudo Genes (internal stop)      :: 25 of 159
            Pseudo Genes (multiple problems)  :: 24 of 159
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079182.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC13_AI2040PC"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pAI2040P_P3"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2016"
                     /collected_by="Christian medical college"
     gene            complement(372..1492)
                     /locus_tag="KWY86_RS27665"
                     /old_locus_tag="KWY86_27665"
                     /pseudo=""
     CDS             complement(372..1492)
                     /locus_tag="KWY86_RS27665"
                     /old_locus_tag="KWY86_27665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611476.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            complement(1681..2673)
                     /locus_tag="KWY86_RS27670"
                     /old_locus_tag="KWY86_27670"
     CDS             complement(1681..2673)
                     /locus_tag="KWY86_RS27670"
                     /old_locus_tag="KWY86_27670"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="WP_087874902.1"
                     /translation="MNKSIESLQVIRAIAACSVVLTHIYGASVPVIGSSGIFGVDLFFV
                     LSGYLMTITYRKNRTGYQFIKSRILRIYPPYLVLSLPLIIFSYFYKNISASDIFHNITL
                     LPTIHGNYDRINFVCWTLTYELYFYIVFSLCMYIFKGARNVILSTSFTMLVLFFLAGGV
                     NGGLAGWKDNSIHHMIGNYLILDFILGSLIGLAYNGYTYKKNAFPILLSIAIIITSCTI
                     IGHIHLPEPYNNYQLITFLTSGIPACVVIYITSKVSVDKIPLKKALIYIGTASYSIYLT
                     HLYAVFAFSKLTHNNAPEKFLCFVTSIVIGCVAYYTIERKIIIKKKNHV"
                     /name="acyltransferase"
     gene            complement(2760..3457)
                     /locus_tag="KWY86_RS27675"
                     /old_locus_tag="KWY86_27675"
     CDS             complement(join(2760..3208,3208..3457))
                     /locus_tag="KWY86_RS27675"
                     /old_locus_tag="KWY86_27675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558270.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1X4 family transposase"
                     /protein_id="WP_223335786.1"
                     /translation="MASISISCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTILRHFKKLRPQSVNSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGELHVISKRYTQRIERHNLNLRQYLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1X4 family transposase"
     gene            3741..3851
                     /locus_tag="KWY86_RS27680"
                     /old_locus_tag="KWY86_27680"
     CDS             3741..3851
                     /locus_tag="KWY86_RS27680"
                     /old_locus_tag="KWY86_27680"
                     /inference="COORDINATES: protein motif:HMM:NF033853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein"
                     /protein_id="WP_139102090.1"
                     /translation="MDWISLAIAIALLVVAVYSGISYFREKKTISRVFRR"
                     /name="small membrane protein"
     gene            4905..5126
                     /locus_tag="KWY86_RS27685"
                     /old_locus_tag="KWY86_27685"
     CDS             4905..5126
                     /locus_tag="KWY86_RS27685"
                     /old_locus_tag="KWY86_27685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016162091.1"
                     /translation="MEFDTAAELAALQAQTRRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLRARRIKVVLSTVTRWLEKNG"
                     /name="hypothetical protein"
     gene            5119..6006
                     /locus_tag="KWY86_RS27690"
                     /old_locus_tag="KWY86_27690"
     CDS             5119..6006
                     /locus_tag="KWY86_RS27690"
                     /old_locus_tag="KWY86_27690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="WP_071579754.1"
                     /translation="MAKFASAARQAAGVIKPLQGNILRSVGTVRNYEQGLTRVAEWMKF
                     NRINHGDLRTLTPAQAITYLEQRGEQVGQKTLDMERQAIQAMMIHVTQTLPAGEHLPVV
                     KSEQAQILSSRAYSPDQVALIAAAQQPANALATAIALAAGLRAHELLTLAPHQERAPDP
                     RPAADSKFAGREGVIYTVQGKGGLVREVLLPSRLAERLEHVRLASPVRVTDRGVFYQSQ
                     YAIGGGQRWSNAFSAASMRTLGWSRGAHGVRHSYAQQRMQELQKLGLVRDMALRTVSQE
                     MGHFRPEITETYLR"
                     /name="integrase"
     gene            6003..6701
                     /locus_tag="KWY86_RS27695"
                     /old_locus_tag="KWY86_27695"
     CDS             6003..6701
                     /locus_tag="KWY86_RS27695"
                     /old_locus_tag="KWY86_27695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071579755.1"
                     /translation="MTRTFILLCTGVFAIAYALYELKHMGVYYPDASNPQLFTGWKAVT
                     ELGPAILATGIAATAFSLWLFILFFRGSESRRIQSETDRYKQALREAEEAHRARQNTLI
                     QNHNLETAALRSHYSGAEDVLREAFAQKESLLLQAVQNARKEAKQELEEQREALNQRES
                     ILQKKELALETTLEKARKYVAGERHNTVLARLEAEKAEERRRNACATAERRRRKLEKLQ
                     TTDGTAGQKH"
                     /name="hypothetical protein"
     gene            complement(6708..7106)
                     /locus_tag="KWY86_RS27700"
                     /old_locus_tag="KWY86_27700"
     CDS             complement(6708..7106)
                     /locus_tag="KWY86_RS27700"
                     /old_locus_tag="KWY86_27700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_071579756.1"
                     /translation="MANVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLDTSE
                     LLRFFGAFAPVSVEQEEEQVSGQRDTVTSDHNEQAIYILKQEIEHLNTLLASKDSHIDS
                     LKQAMLLLENREEKRQNTNRPWWRFWQS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(7106..7297)
                     /locus_tag="KWY86_RS27705"
                     /old_locus_tag="KWY86_27705"
     CDS             complement(7106..7297)
                     /locus_tag="KWY86_RS27705"
                     /old_locus_tag="KWY86_27705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_071579757.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAGADLCEKLHDDA
                     EHLFRTLSLRLDALQEGD"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          7346..7819
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.795"
                     /coverage="92.0"
                     /evalue="3.49e-179"
ORIGIN
        1 tgatttagag gagatagtct tgaagttatg cgccggttaa ggctaaacta aacgaacagt
       61 tttggtgact gtgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga
      121 accttcgaaa aaaccgccct gcaaggcggt tttttcgttt actgagcaag agattacgat
      181 gctaaagaaa ggatctcaag aggatcctgt tccatatgcc tacccctgaa gtgaaaccag
      241 caccactccg cgacaccaac catcaaaata aaactattta actcgttgtt tttgataaga
      301 aaaaaacata tataatcatg atccataatc atccaacgcg gttgacctgc ccccaggatt
      361 agatacaacc ttcagttagt aatgtcggtt cgtttttctt catatttccc gtttcgccag
      421 cccgccgcaa attcagccgg agtctggtag ttcagcgatg aatgtggacg acactcgctc
      481 taatcctgcg gccagtcatt aatcgttttc ctcgcgtgaa cgatatcgcg gaaccagtgc
      541 tcattgaggc actcatccct gaatcgtccg ttaaaacttt caataaatcc gttctgtgtt
      601 ggcctgcccg gctggataag ccgcagctcc accccatgct cataagccca ctggtcgagt
      661 gctctgcagg taaactccgc cccctggtca gttcttatcg tcgccggata gccgcgaaac
      721 agtgcaatgc tgtccagcat tcgcgtgacc tgaacgcctg aaatcccaaa ggcggcggta
      781 attgtcagac actccttcgt gaagtcgtcc acgcaggtca ggcacttgat cctgcgaccg
      841 gtggccagcg cgtccatgac aaaatccatc ggccaggtca ggttgggcgc ctccgggcgg
      901 agcagcggca gacgttctgt tgccagacct ttacgacgtc gtctgcgttt tacacccagc
      961 ccgttaagat ggtaaagacg gtacacgcgc ttgtgattaa catgaaggcc ttcacggcgc
     1021 agtaactgcc agatgcgtcg gtagccaaaa cgcctgcgct ccagtgccag ctcagtgatg
     1081 tgccctgata aatgcgcatc agccgcggga cgctgagcct catagcggca ggtcgacagg
     1141 gacaaacctg taagcctgca ggcacgacgt tgcgacagac cggtcgcatc acacataaac
     1201 tcaacggctt cccgtttctg gtctgtcgtc agtactttcg ccccagagcc acctgaagtg
     1261 cctccttatc cagcatggct tcggcaagca gtttcttgag tctggcattc tcttcctcaa
     1321 gcgacttaag gcgcttaacc tcgggcacct ccataccgcc atacttctta cgccaggtgt
     1381 aaaagatggc gtcggaaatg gcgtgcttac ggcagagctc acgggcagaa accccggctt
     1441 cggcctcgcg gagaatactg atgatctgtt cgtcggaaaa acgcttcttc atgaggatgt
     1501 cctcatgtgg cttatgaaga cattactaac atcacggtgt attaaccaac ggggagcagg
     1561 tcactccgtg tatgccccgg ttttttcgcg ctgtcgctgt ctaaactggc tgtaaaaatt
     1621 tacgcctact gggatgggct cgcagtaggt tcattaacca attattaaat gctcatgtta
     1681 tcaaacatgg ttcttttttt ttattattat cttcctttct attgtgtaat atgcaacgca
     1741 gcctattact atcgaagtaa caaaacatag aaacttttca ggtgcgttgt tatgagttag
     1801 tttagaaaat gcaaacacag cgtaaaggtg tgttagataa atggagtatg aagctgttcc
     1861 tatataaatc aatgctttct ttagaggtat tttatctaca cttacctttg atgttatgta
     1921 tatgactacg catgctggaa tcccagatgt tagaaatgta atgagttggt agttattata
     1981 tggctcaggt aagtgaatgt ggcctataat ggtgcaactc gtgattatta ttgctattga
     2041 tagcaatata gggaatgcat tttttttgta tgtgtaccca ttgtacgcca atccaattaa
     2101 ggagccaagt atgaagtcta atattagata gttaccaatc atgtgatgaa tagaattatc
     2161 tttccagccc gctaatccgc cgttaactcc acccgctaaa aaaaataaaa caagcatggt
     2221 gaatgaagtg gataagataa cattccttgc ccctttgaat atgtacatgc acaatgaaaa
     2281 tactatgtaa aaatataatt catacgttaa tgtccagcaa acaaagttta tcctatcata
     2341 gttaccatga atagttggga ggagggttat gttatgaaaa atatcagatg cacttatgtt
     2401 tttatagaaa tatgaaaaaa taataagagg cagtgataac acaaggtaag gtggatatat
     2461 tcttaatatt ctgcttttta taaattgata ccctgttctg tttttgcggt atgtaatagt
     2521 cattaaataa ccgcttagca caaaaaacag atcaacacca aagatacccg aagagccaat
     2581 tacaggtacg cttgcgccat aaatgtgtgt aagaactact gaacaggctg ctatggctcg
     2641 aataacttgt agtgactcga tacttttatt catgaattta catactgaaa tgggttattg
     2701 aggtatttta ctggaaaaaa ctggatgtat cactaattac ttgtggtgat gacttcaact
     2761 tactgatagt gttttatgtt cagataatgc ccgatgactt tgtcatgcag ctccaccgat
     2821 tttgagaacg acagtgactt cctgcccagc cttgccagat actgcctcag attcaggtta
     2881 tgtcgctcaa tgcgctgcgt atatcgcttg ctgataacgt gcagttctcc cttcaggcgt
     2941 gattcataca gcggccagcc atccgtcatc cataccacga cctcaaaggc cgacagcagg
     3001 ctcagaagac gctccagtgt ggccagagtg cgttcaccga acacgtgcgc cacaaccgtc
     3061 ctccgtatcc tgtcatacgc gtaaaacaac cagcgctggc gtgatttagc gccgacgtaa
     3121 ccccactgtt cgtccatttc cgcgcaaaca atgacgtcac tgcccggttg tatgcgtgag
     3181 tttaccgact gcggcctgag ttttttaagt gtcgtaaaat cgtgttgagg ccaacgccca
     3241 taatgcgtgc actggcgcga catccgacgc cattcatggc catatcaata attttctggt
     3301 gtgtaccggg ctgagaggcg gtgtaagtga actgtagctg ccatgtttta cggcagtgag
     3361 agcagagata gcgctgatgt ccggcagtac ttttaccgtt acgcaccacg ccttcagtag
     3421 ctgagcagga aggacaactg atggagatgg aagccacggg agcacctcaa aaacaccatc
     3481 gtacactaaa tcagtaagtt ggcaccatta ccgtgatttg ttatataccc accacggggg
     3541 cattagtttt tttatttttt tgatgatatg tacaaaatca gtggttagga ataatcttag
     3601 cagccttaac aaacacatac aaaaacagcg gttatgtttc atctcaccac aataagtaac
     3661 gacagtatca tgctaaaaaa agatgacatt atctttatga aatcataaca ggctgttttt
     3721 attaaattaa tcaggcattt atggactgga tttcacttgc tatcgccatt gctctacttg
     3781 ttgtagctgt ctactcagga atctcatact tcagagaaaa aaaaacaatc agccgtgtat
     3841 ttaggcgata gcgccctatt taccataatc ccttcttacg cgcaccgcga aaacggcctc
     3901 agggcgtttc tggtggttaa cgggcaaaag gtggtggcaa gtacaccgaa aaccgccaga
     3961 aacgcgcctg atttatttta cgcatatgca tggctatgca caccaccgca tagccggtga
     4021 tttcggccga gaaaagggtt ctggacgtta aaagcataaa agaacgggcc atccaggccc
     4081 gtcaaatgag gacaactctg taaaaggacc gtcgcacctg gtgcggtgcc ggcgacaaga
     4141 aaaagatagc tcatatcagc ccctcaccgg agaaaaatat ttcctggcca cctcaaccgc
     4201 cacacgctgt gttcccgtta actcgcgcag ataacgcagg aacatcctgt tttcctctgc
     4261 atgcgcctcc tcactgccct gtgcgtggta ttccactatc gcgttcacca cggactcacc
     4321 gacctgcatc gtctgacctc atctgcactt tcatcacacc gtttcagatc ccgcctcctc
     4381 gccatgatgt gaaacggtga ccgggcaggc aaattttttg tctgtccggt caccgttggc
     4441 ggggtccggg gcggtcagtc ccggtgtttt ttattccgcc aaatcagcac ctgcgacacc
     4501 cgctatgtac gacgcataaa atgcgcctgt acatagcggg tgtcgctatt aaactgagtc
     4561 aggcgcaaca tttaaaaaac aacattcaca cgcacgaaaa acctggctag tatttagtca
     4621 aaccatgagg aggcgcactc tgacgcgcac ctgcctaccg gcagcaggta aaacagccgg
     4681 gtccccccgg ggatactgta ccggacagcc caatccgaga atcctgagaa aacagccaaa
     4741 atctgcgaga ccggcaatca ggtcaggcca ttccctggct taagggacgt ttttacagcc
     4801 gcgagcagtc cgggcttaac cacgttacgg tttaaacgag ggcgatcggc gcaggccgca
     4861 gtggatttcc actggcgttc ttacaactca acaccggagt tcctgtggaa tttgataccg
     4921 ccgccgaact ggccgccctg caggcgcaga cccgccgtat ccgccaggtc cgctaccgtc
     4981 cctcccgcct tgaccgctat accggcgaac tgctgagcct gtaccaggcc ggggccagtg
     5041 cagcggagct gcaacgctgg ctccgggccc ggagaatcaa agtcgtgttg tccacggtta
     5101 cccgctggct ggaaaaaaat ggctaaattc gccagcgctg cccgccaggc ggctggcgtg
     5161 ataaaaccac tccagggaaa tattctgcgc tctgtcggta cggtccggaa ttacgagcag
     5221 ggactgaccc gcgtcgcaga gtggatgaaa tttaaccgca tcaaccacgg tgatttacgc
     5281 acactgacac cggcgcaggc catcacctac ctggagcagc gcggggagca ggtcggccag
     5341 aaaacgctcg atatggaacg ccaggccatc caggccatga tgatccacgt gacgcagacc
     5401 ctccctgccg gcgaacacct gccggtggtg aaatcggagc aggcacagat cctgagcagc
     5461 cgggcctaca gcccggacca ggtggccctg attgccgcag cacaacagcc cgccaacgcc
     5521 ctggccaccg cgatcgccct ggccgccgga ttacgcgccc atgaactgct gaccctcgcg
     5581 ccccaccagg agcgtgcacc ggatccccgt ccggcagcgg acagtaaatt tgccggtcgg
     5641 gagggtgtga tttacaccgt tcagggcaaa ggcggcctgg tcagggaagt tctgctgccg
     5701 tcacgcctgg ctgaacgtct cgagcatgtc aggctggcca gcccggtccg ggtgaccgat
     5761 cgcggcgtgt tttaccagag tcagtacgcc attggcggag gccagcgctg gtcaaacgcc
     5821 ttcagtgccg cctccatgcg taccctgggc tggtcacggg gggcacacgg cgtgcgtcac
     5881 agctacgccc agcaacggat gcaggagctg caaaaactcg gtctggttcg tgatatggcc
     5941 ctgcgtaccg tgagccagga aatgggccat tttcgtccgg aaatcacgga gacatacctg
     6001 cgatgacaag aacgtttatt ttattgtgta ccggtgtttt cgccatcgcg tacgccctgt
     6061 atgagctgaa acatatgggc gtttactatc cggatgccag caatccgcag ctgttcactg
     6121 gctggaaggc ggtcaccgaa ctcggtcccg caatcctggc tacggggatc gcagccacag
     6181 cgttctcgct gtggctgttc attttgtttt tcagaggctc agagtcccgc cggatacaga
     6241 gcgagacaga caggtataag caggctctga gggaggcaga agaggctcac agagccagac
     6301 aaaataccct tattcagaat cacaacctgg agacagcggc cctgcgaagc cactacagtg
     6361 gtgctgagga tgtactgcgg gaagcgtttg cacaaaaaga aagcctcttg cttcaggcgg
     6421 tgcagaatgc ccggaaagaa gccaagcagg agctggaaga acagagggag gcactgaatc
     6481 agcgcgaatc catcctgcag aaaaaggagc tggcgctgga aacaaccctg gagaaggccc
     6541 ggaaatatgt ggccggggag cggcataaca ccgtgctggc ccgtctggaa gcagagaaag
     6601 cagaggaaag acggagaaat gcctgcgcga cggcggaacg gcgcagacgg aaactggaaa
     6661 aactccagac cactgacgga acagcagggc aaaaacactg aggcatttca ggactgccag
     6721 aaacgccacc aggggcggtt cgtgttctgc cgcttctctt cccggttctc cagcaacaac
     6781 atcgcctgtt tcaggctgtc gatatgtgaa tcttttgagg caagaagggt gttcaggtgt
     6841 tcaatttcct gtttcagaat gtaaatggcc tgttcattat ggtcagaagt gactgtgtca
     6901 cgctgtccac tgacctgttc ttcctcctgt tcaacggata cgggcgcaaa ggcaccaaaa
     6961 aaccgcaaaa gctctgaagt atcgagcatt ttcgcattcc gttcaccccg acaggtggac
     7021 agttcaccgg ctttaattaa cctgtgcagg gttgtcctgc tcttacccgt caggcgtgct
     7081 gcctcactga tcgacacgtt cgccatcagt caccctcctg tagtgcatcc agtcggaggg
     7141 acagtgtacg gaaaagatgc tccgcatcat cgtggagttt ctcacaaagg tcagcccccg
     7201 catcgagatc aagctcgtta agcttctcca gcaggagcag ggactgattc tggatgaatt
     7261 tagccatgtt cagggcagtt ttctgttgtt tcgtcataag gggcctcagg gcgtcagtgc
     7321 tcagtgtaaa accggtctct tttccgcttc ctcgctcact cagtcgctac gctcctgccg
     7381 ttcggctgcg gcgagcggac gggacacaca gaagagtcat cgctatcacc aggaggagaa
     7441 gagcctgtgg aaaaggccac ggcgttgccg tttttccata ggctccgccc ccctgacaag
     7501 catcacgaaa tctgacgctc aaatcagcgg tggcgaaacc cgacaggact ttaaagatac
     7561 caggcgtttc cccctggtgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc
     7621 ggtgtcattc cgctgttatg gccgcgttta tctcattcca cgcctgacac tcagttccgg
     7681 gaaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgactgctg
     7741 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacatgcaa aagcgccact
     7801 ggcagcagcc actggtaac
//
