LOCUS       NZ_CP079167             9730 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC09_CMC309PC
            plasmid pCMC309P_P3, complete sequence.
ACCESSION   NZ_CP079167
VERSION     NZ_CP079167.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20115187
            Assembly: GCF_019286955.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC09_CMC309PC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC09_CMC309PC
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 91.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:01:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,418
            CDSs (total)                      :: 5,302
            Genes (coding)                    :: 5,153
            CDSs (with protein)               :: 5,153
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 67 of 149
            Pseudo Genes (incomplete)         :: 81 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 23 of 149
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079167.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC09_CMC309PC"
                     /isolation_source="Endotracheal aspirate"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pCMC309P_P3"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2018"
                     /collected_by="Christian medical college"
     gene            join(8977..9730,1..1436)
                     /locus_tag="KWY74_RS26790"
                     /old_locus_tag="KWY74_26790"
     CDS             join(8977..9730,1..1436)
                     /locus_tag="KWY74_RS26790"
                     /old_locus_tag="KWY74_26790"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WP_063938521.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     gene            3022..4983
                     /locus_tag="KWY74_RS26795"
                     /old_locus_tag="KWY74_26795"
     CDS             3022..4983
                     /locus_tag="KWY74_RS26795"
                     /old_locus_tag="KWY74_26795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_063938523.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            4983..5714
                     /gene="mobC"
                     /locus_tag="KWY74_RS26800"
                     /old_locus_tag="KWY74_26800"
     CDS             4983..5714
                     /gene="mobC"
                     /locus_tag="KWY74_RS26800"
                     /old_locus_tag="KWY74_26800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071653262.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            5721..6251
                     /locus_tag="KWY74_RS26805"
                     /old_locus_tag="KWY74_26805"
     CDS             5721..6251
                     /locus_tag="KWY74_RS26805"
                     /old_locus_tag="KWY74_26805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938511.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6279..6458)
                     /locus_tag="KWY74_RS26810"
                     /old_locus_tag="KWY74_26810"
     CDS             complement(6279..6458)
                     /locus_tag="KWY74_RS26810"
                     /old_locus_tag="KWY74_26810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6527..6904
                     /locus_tag="KWY74_RS26815"
                     /old_locus_tag="KWY74_26815"
     CDS             6527..6904
                     /locus_tag="KWY74_RS26815"
                     /old_locus_tag="KWY74_26815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938513.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="hypothetical protein"
     gene            complement(6966..7265)
                     /locus_tag="KWY74_RS26820"
                     /old_locus_tag="KWY74_26820"
     CDS             complement(6966..7265)
                     /locus_tag="KWY74_RS26820"
                     /old_locus_tag="KWY74_26820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_063938515.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7255..7518)
                     /locus_tag="KWY74_RS26825"
                     /old_locus_tag="KWY74_26825"
     CDS             complement(7255..7518)
                     /locus_tag="KWY74_RS26825"
                     /old_locus_tag="KWY74_26825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938517.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            7895..8980
                     /locus_tag="KWY74_RS26830"
                     /old_locus_tag="KWY74_26830"
     CDS             7895..8980
                     /locus_tag="KWY74_RS26830"
                     /old_locus_tag="KWY74_26830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WP_063938519.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     Typing          complement(1642..2222)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.625"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4984..5711
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.885"
                     /coverage="100.0"
                     /evalue="3.84e-144"
ORIGIN
        1 ttacgattgg gggatatcag cagtatgaat ataacaatca ttggtggtat gacttaccta
       61 tttctggtac cggacaccac cttaatcttc ctcgctcgac gtttacaggc aatgactggg
      121 agtttgaaag aagtaaaagt aaaactgcat ttgcaactct tgagcagaag ttcagtaata
      181 gctggaagct gaaattttca acgctgcaaa actggagaaa tgctgacctg ttagggacgg
      241 caacataccg gcataaagat acccgacaaa gtatgtatca gcaagtatgg ggatcactga
      301 gggaatataa ttccaaaagc tatgacctgt cgcttaatgg ggactatgct ttatttggtc
      361 gtgttcatca attcgtggct ggaacaactc agcaaaaata caccgataaa gatacaggtt
      421 atacatattc cccaatatat attgagaatg ttaatatatt tgatcatggt tcacattctt
      481 cgaaaaaacc catgaagatg tcaggtgatg catcctacga cgtcactgaa caaaaaagcg
      541 cctacgcagc aactcatttt ctacttagtg ataactggaa aatattgctg ggtggccgtc
      601 ttgactggta tgaatataca agtaaaggta catccattaa tacaggttat aaagttacca
      661 gaaatatcac caaatatgca ggttcaacat acaatcttga cgatcatcat accatttatg
      721 ggagctatac cgatatattc aatccacagt caagcaccga cctcaatggt aaagtgatca
      781 agcctattgt tggtaagaat tatgagacag gtattaaggg tgagtatttt aacggcttgc
      841 ttaatgcctc tctggcggta tttcaggtag accaggaaaa tcgagccagg ctgcttcccg
      901 atcagacagg gtgtgttact aatggcaatc ctcaatgcta tgaggctagc ggtaaagtac
      961 gcacaaaagg gatagatttc gaacttcagg gcatggttac gccactctgg gaaattgggg
     1021 tgggatatac atatgcagat acgcgatata taaaagatgg agatcgtagt aatgaaggca
     1081 agcgattcaa tactgccgtg tcgccacaaa atttatttaa gatttcgagc atgtacacct
     1141 ttgacggaaa attaacaaac tggcgagcag gagtaaacac gtactggcaa agtaaaatat
     1201 atgctaacat aacagacaca agggatggca atccggttat taataaacaa aaggcttatt
     1261 caacaactga cattattatt ggctatactc caatgccaca actggattta cagctcaata
     1321 taactaatgt ctttgataag gtttattata catccatagg tgaatcgatt ggatatggca
     1381 cggaaacata tggagaacca agaaatgtta aaattagtgc aacctataga ttctaaccat
     1441 gcggattggg tcttctcctc ggatttgccc ctataaatcc gtcttttgtg gttccggcgt
     1501 acaaccccct gtttgccact tcagttgatg gttaatcaaa tcatgtagag gatcttcttg
     1561 agatcctcca tggttcgtcg taatcttttg ccctgtaaac gaaaaaacca cctggggagg
     1621 tggtttgatc gaaggttaag tcagttgggg aactgcttaa ccgtggtaac tggctttcgc
     1681 agagcacagc aaccaaatct gtccttccag tgtagccgga ctttggcgca cacttcaaga
     1741 gcaaccgcgt gtttagctaa acaaatcctc tgcgaactcc cagttaccaa tggctgctgc
     1801 cagtggcgtt tttgcgtgtc tttccgggtt ggactcaagt gaacagttac cggataaggc
     1861 gcagcagtcg ggctgaacgg ggagttcttg cttacagccc agcttggagc gaacgaccta
     1921 caccgagccg agataccagt gtgtgagcta tgagaaagcg ccacacttcc cgtaagggag
     1981 aaaggcggaa caggtatccg gtaaacggca gggtcggaac aggagagcgc aagagggagc
     2041 gacccgccgg aaacggtggg gatctttaag tcctgtcggg tttcgcccgt actgtcagat
     2101 tcatggttga gcctcacggc tcccacagat gcaccgaaaa agcgtctgtt tatgtgaatt
     2161 ctggcaggag ggcgaagcct atggaaaaac gccaccggcg cggccctgcc gttttgcctc
     2221 acatgttagt cccctgctta tccacggaat ctgtgggtaa ctttgcgaga gttagcagcg
     2281 cccgccgcag tctcacggcc ggagcgtagc gactgagtga gggaggaggc gcaatatccc
     2341 ccctcccgca gacggccacc ggacgcgcgg atgtgacata ttgcaccgtg ttaccgcctt
     2401 tcccttccct gccgataaat cattcagata ggtgaagtct gtcggttttc tgctgaatat
     2461 tttacgttaa cctatctctg ttgctttttt ctccttgcgg agaaatgcag gtcatccccg
     2521 ctggggcgtg gctgacttca ggaggaaatc ccctgcttac gctggctgga cgccaccgac
     2581 gcacggtctt tccccctctt tctggctcag ctcggctgga cgccggccac gccagaaatt
     2641 cacccggccc ccgtcaggct cacgcctgac cacccacaaa aacccacggt gctgaacgca
     2701 cctgacgaag cagcaaagct gctcctgatt ttgcgggtgg tcagccgctc gctatggcga
     2761 actctgacgg gggccggaac accggaaaag accgcactgg ggtgccgcct tttcctctca
     2821 cacgctgggg cgtgctcgtt gttcctccgg ggctgcgcct tttccgcttg gggcggaaag
     2881 aggctggcct ccgtggtgaa agctgaatgc aatcaccacg gacgtgaccg ccgccgccat
     2941 gcctcggggc atgtccgcgg cttgtgcagc aggggctgca caccacgccg gtaaacccgt
     3001 cgtctgaagg agagagattg catgtttaat acggattcgc ttgcctggca gtggcatatc
     3061 ccggagctgg cccggcagat ttacccgtgg gtcagctggc tgtacaggga gtattacggt
     3121 ctggccgggg ctttcctggt tatcggcctg tttttcggtc tggctgaact ctgggccgga
     3181 cggatgcgtt ttgtggggct gagtcataag gagcgacggg agtatgccga tcgctataag
     3241 cgcgataata accggactgc gccattttcg gtgctggctt cgctggattc gcccgggcgg
     3301 atggtcaggg cacggcggca tgtctggaac tacgcgtact ggctgaccgg cgctgccggt
     3361 ctggcccttc tgggggggct ggttgtcgcc gtgtttatcg agagctgggg agaaaccgtc
     3421 ctgcgtccgg acctctacgc gaacgcagat tattacctgc tgcagccggc taacctgttc
     3481 gggattgtct ggtgtgccgg agggcttgtc gccggcatcc ttctggcgcg gctgctggca
     3541 tttctgctgc ttgacggtca ttttgcggcc gctgacgagg cggtgaatgc gaaactgaac
     3601 caggagagcc ggcgcagcag ccagcggacc ggagaaatga ccgacgtccg ccacctgcat
     3661 ttcggggagc cggtaccggt taacgcgctg gccgattttt ccacagagca ggcccggaaa
     3721 cagcaggcgg tgtttctggg taaggatgag cagggccagc cggtgctggt cccgcgtgat
     3781 acctggcgca agaccaatat tcagatcctc ggcctgcccg ggagcggcaa gagcgtgatg
     3841 ggcaccaacg cgctcattcg ctgcgtacgc gatttcgggg acgcggtggt gtacttcgac
     3901 cccaaagggg acgcgtgggc gccgcacgtc ttccgggcgc actgcccgga cttcaccctg
     3961 ctggatctgc ggccggggaa accggcgcag ctgaatctgt ttcgcgatct cgaccagtac
     4021 gccctgaaga atctgctggt tgccgggttt aacctgagtg agaccggcga cgtggcggac
     4081 cactaccgca tcagcgagca gaaagcggcg aagctgattg cggagcagtt cccgcagggg
     4141 gcaaacattc agcaggtgct ggcggcggca tacgcgctgc cggaggccct gaaaaaggac
     4201 gtgaaggggc tgatcaccaa gctggagaac gtggccgacc tgagcgtcct gcagacggac
     4261 agcggcatcg acgtggcggg gataatcaac ggcggcggct gcctgtacgt catcggctcg
     4321 atggacgatg aggcggtgat ccgggtgcag aagatgctgt ttgcccgctg cgcgcaaatc
     4381 atcattgcgc gggatgagtt ccggaggtgg ccgcacgcga gcatcatgct cgatgagatt
     4441 aaatacctgc tgtcgaagta cgtgctgaac gcgctgggga cgctgcgcag tcgggactgc
     4501 aacctgctgc tcgcgcacca gtcgctgggc gacttcgggc agtgcggcca ggatctgccc
     4561 gctgagtttg tgaaaaccac ggtgctggat aacacgccga tccgctggtt ctaccgggcg
     4621 gccagccagg agtccgcgca gtgggcggcg ggccagaccg gggaaatccg ggtggacgtg
     4681 gagcgccgcc gggccagccg ggaggcgggg aacgtggagc atatcagcgg ggacagcttc
     4741 attcagaagg aggcgcggcc gctgtttgac gtgaacaccc tgcagcacct gcctgacggg
     4801 tttgcggtga tgaccgggct gggcgtggcc cggatggcgt tcagcagccc gctgcgggtt
     4861 gaacgccggg aaatcccgct gcagcgcttc ccggtgctgg cgaaaacgga cccgctggcg
     4921 gagtaccagc cggaggccgg gcggcagcgg ccgggtgatg atgattttgc ggggctgtac
     4981 tgatggcact ggagcgatac aacgtcagcc acgcgaagcg gcaggcgcgg aacgcggaga
     5041 aaacccggct gaccctgcgc tggctgcggg aagagctgtg ttccacggcg gagctggttg
     5101 cgcgacggct ggggattgcg gcggtgcagc cggtttaccg cttcctggac agcctggtgg
     5161 cgaaagggct gctggtccgg gcgaaatacc cggttgacgg ccgtcaggtc agcgtgtggg
     5221 gactgacgcc gcacggggtg gcgttctcgt tcgacgagga cgaaccgctg acggacgtta
     5281 ttccgtttca gccgtcaagg gtgtcggcgg cgcagctgcc gcaccggctg gccgtgcagt
     5341 ccctgcgtct cgccatggag gcccgggggg cctccggctg gcggtacctg caccgtatgg
     5401 ccctgaaggg gatgaaggtg ccggacgcgc tggcggagct ggacggcaga acggtggcgt
     5461 ttgaggtgga gcgcaccgtg aagagccgca ggcgctatca ggaggtggtg tccgggtatc
     5521 tgttcaatcg caaagcgaac ggcatcgatg aaatctggta catctgcccg gaccgggcga
     5581 cgcaggtcag ggtgcagcgg gcgatcctgt cggtggatga aatcgtgaat ccacagaccg
     5641 gcgaggcccg caaaacggcg gagctggacc gggagcggct gtttgcgtgc tttaaattta
     5701 tgacaacgga gtgagggatt atggcgctgt acatggtatt tattgaagac gcggaggacc
     5761 gggacggcga gcgctgcatc atgccgacca gcgagcatct tgaagatatg gtggtgctcg
     5821 cctggcagac cggggcgtat gttttctgtt ttgtcgaccg ggcccagcat ctggcgatga
     5881 tgaaatccat cgggatgtat gacgagcggc tcaacagcga cgaaaaagcc acgtactttt
     5941 tcaccggtaa aaatccgcaa tgggaacggg acaaacccgc aggaatgaag ctgtgtgatt
     6001 acctctatcg caaggtggag cggttcgcca tcgagaacgg tgtgcaggtg gttgatgccg
     6061 ggaagtgctc accggagcag attagcgcga tgattgaggt ggtcagcgag cggcacgggg
     6121 aaagcgcggt gaacgagaag ctgaaagcgg agctgatgcg ggaatataac agccgctatt
     6181 ttaacgtgcc ggtgcctgaa ctgccggagg acaaacccgc ccgccggtgg tggccgttcg
     6241 gcaaaaaata aacggcagca ccggccatgc cggtgctgtt attccttccc gagcttatcc
     6301 cgcatcacct gccagagtgt ttccgcatcc tcgtgcagcc gctcgcacat gtcggccggg
     6361 gtatccatat ccagctcgtt cagtttctcg agtaacagca gcgactggtc cttaataaat
     6421 ttagccatat tgagggcagt tttttcctgc ttagtcatgt tttctgtcct tccgggtgtg
     6481 gtgtgcttcc tgtcgggtat actgtctcaa aaccggaggt gagacaatgg cgtgggtgac
     6541 ggtgagacag gcgacggaac tgacggggaa agcccggagt tccctgtata gggatatggc
     6601 gaaaggccgc gtatcatacc gtactgaagc tgatggtggt cgggtggtgg ataccagcga
     6661 acttatccgg gtgtacggtg aattaagaca gattgagact cacgagcggg acgggttgag
     6721 actgtcggat gagactgaaa aaatggcctc cgacgcgttg atcgcggaga taaaagcact
     6781 cagggaggag gtggccggac tgcggcagga aatgcaggcc atgagactgc tggaacacaa
     6841 accagagaca gcagagacac cggccggaac atcgcgccga tggtggcagt ggggaaaacg
     6901 ctgacgcgct gtgcttgtcc aaccggcgac ggccacgcaa tgaattgcgc ccccatcacc
     6961 ggttatcagc gaacggtatg gggattctgg cgctggccaa ccacggccag aacccggact
     7021 ttcctggtct ggtcatcaac atcatagagg acatgctgtc ccatcagact gatccggcga
     7081 acgtcctcgc catagcgtga gtccacttca tataggcgcg ggaagtcggc cagtttgtcc
     7141 gctgcagcga gaactcgatc ggtaacgttc tgcgcatccc acagggatga attttccgca
     7201 atgtagtcaa acaatgctgt cacatcctcg atggctccct gcttccattc cacctcatac
     7261 ggcatcgcgt agttcctgat agcggccatg cagtctctgg cggagttgtt ccgtggtcat
     7321 gcccgggcgc gtgtcagcgc gggcggcggt cactttagcg cgaacctgat cttcatattc
     7381 tgcctgggcc agttcttcgt tagccaggcg ctgcaacagt gcagctttgt ccgggccacc
     7441 ttcaggcagt atgcggggca gtctctcggc cagttcccgc aggaaattgg cgttttcata
     7501 gtgtgtactg ttgctcattg tggagcctcc cgggctgata cgtcgatatg aaggagttta
     7561 gcagggggcg gtctgaaaga atatgctcgt gtcagtcagg ctgatgaaga cgaaagctgg
     7621 taaaaaaggg cttacgacac ccattttacg tcaaactgca cgacatgcag ttgcgtgcat
     7681 agccatgcag cgggttaaaa tttatcaggc gcgtttccgg cggttttccg ggtggtttgt
     7741 tgccggtttt atcccgtagc cgccggaaat gccctcagac cgtctgagcg gtgcgcgtaa
     7801 tgcgacgtta tggtaaatca tgtgattaat ggtagtgata atcattatca cttttgctat
     7861 attcttgttt cttttattgt aaggaaatgt aagtatggca atcacgatta aatccgccgt
     7921 taatgtccgt agcagagtat taatatactc tttactactg tttactggtt cagcgtatgc
     7981 ggctgatata gctgaatcaa cattcctgac tcgtgatatc ggcaatggtc tgtatgaaat
     8041 gtcttttgat ggcaaaaata atagtctctg ggcagcatca tcgtcttcat tcgacagaga
     8101 taagggccac ggaattgttt atcagttaga accatccact ctgaaaacaa aatctgccgt
     8161 taaaatcgca cgtcttgggt ttgccacagc actggatgag gaacatgagc gactttatat
     8221 cggcaatact ctggacggct cagtgacggt cattgatact ctaaccggaa aagaactttc
     8281 agtaattccg ttaactgagg gacaggatga ggctcatttc agaccgacca gagaaatggt
     8341 cattgacaaa aaaaatcagc gtctttatgt ctccagcgcc gcagataaag gggctctgtg
     8401 ggttattgac atggctgtcg ggaagaaaat taaaaccatt gatggactgg ggcacgctgt
     8461 tactggaatt gcgttaaata aaaatgggga taatatttat ctggttaacg gggatggtga
     8521 gattattaac ctggatgcca gtagctatga aataaaaaaa cggtttgttg tagagcctga
     8581 gaaaaaacac tttttcctta atgtaagtct ggatgaaaaa aacggacgtg catttattac
     8641 cgacccggat attcccgggg tgctggttgt tgatgtaaac tcgggaaaaa tactacacag
     8701 aattgatgtt ataaattcac tggcgatttt atttaatcct ttacggaacg aaatctatat
     8761 cacgcaccgt aatgcaaaac aggtcagcat tgtggacagt aattcttatt acgtaaaaac
     8821 aacgataaaa acccgcctga tgccaaacag tctgtcttta tcacatgacg gtgaaaatct
     8881 gtacgttagt gtaaaacaag aaaaaaaaga gatgagtact atgaaggatt acatactcaa
     8941 aatcgaatta tctaaaattg ctatggagcc agaagaatga ataaatattt agggctgagc
     9001 atagtatata tgatgtatac atcatctgtt atggctgatg attctggcgc agttagtaaa
     9061 gataaaaata tttcaccaga agaaacatta attgttcatg ggaccgctgt ttctgaaagc
     9121 acaacagaag gaacagattc atacacaacc cagtcagcca atacagcatc aaagatgaat
     9181 ctgtcattcc gggatacacc tcagtcaact acagtcatta cgcgacgaag aatggatgat
     9241 gtagcagcaa aaaatattat tgatgtggtt aaaaccgctc ctggattata tgtcagtaca
     9301 gcagaaggtt ccggaagacc gaattttatg tccagaggat ttaatgctaa tgtcatgtat
     9361 gaaggattta gcctgccgtg gaattcatac attccaagtt cacaaactag ttcagctatt
     9421 tttgaccggg tggagatagt ccgcggggca acaggtcttg ttcagggtgc cggagaccct
     9481 tcagccgcga taaatatgat acacaagcgt ccgacccggc aatatcaggc cagcactaac
     9541 atcggtgccg gacggtggaa tgactaccgg ggtggggttg atgtatcagg accactgaat
     9601 gacagcgcga ccttacgcgg aagggcagtt gtggatggcg aaaacagtga gaccttcagg
     9661 gatagtgaga aacgcaggaa taacgtcatt tacggaatta tggaggcaga tctgactgat
     9721 aataccctac
//
