LOCUS       NZ_CP079163             1044 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC08_CMC432MC
            plasmid pCMC432M_P8, complete sequence.
ACCESSION   NZ_CP079163
VERSION     NZ_CP079163.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20115186
            Assembly: GCF_019286855.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Complete genome of Carbapenem resistant Klebsiella
            pneumoniae_WRC08_CMC432MC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method :: UNICYCLER v. V.0.4.8; CANU v. V.2.1.1
            Assembly Name                     :: WRC08_CMC432MC
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 91.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:00:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,830
            CDSs (total)                      :: 5,711
            Genes (coding)                    :: 5,529
            CDSs (with protein)               :: 5,529
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 71 of 182
            Pseudo Genes (incomplete)         :: 113 of 182
            Pseudo Genes (internal stop)      :: 26 of 182
            Pseudo Genes (multiple problems)  :: 23 of 182
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079163.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC08_CMC432MC"
                     /isolation_source="Endotracheal aspirate"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pCMC432M_P8"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2018"
                     /collected_by="Christian medical college"
     gene            complement(join(1024..1044,1..978))
                     /locus_tag="KWY73_RS28970"
                     /old_locus_tag="KWY73_28970"
     CDS             complement(join(1024..1044,1..978))
                     /locus_tag="KWY73_RS28970"
                     /old_locus_tag="KWY73_28970"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="WP_218804501.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMVEVIEPFY
                     PKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTT
                     IMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPE
                     MHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAG
                     YQGAPQREELAEVDVDWLIAERPGKVKTLKQHPRKNKTAINIEYMKASIRARVEHPFRI
                     IKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQWERSQKVRTSS"
                     /name="IS5-like element ISKpn26 family transposase"
ORIGIN
        1 ctgagatctc tcccactgac gtatcatttg gtccacccga aacaggttgg ccagggtgaa
       61 taacatcgcc agttggttat cgtttttcag cagccctttg tatctggctt tcacgaagcc
      121 gaactgccgc ttgatgatgc gaaacgggtg ctccaccctg gcacggatgc tggctttcat
      181 gtattcgatg ttgatggccg ttttgttctt gcgcggatgc tgcttcaagg tttttacctt
      241 gccgggacgc tcggcgatca gccagtccac atccacctcg gccagctcct cgcgctgtgg
      301 cgctccttgg tagccggcat cggctgagac aaattgctcc tctccatgaa gcagattacc
      361 cagctgattg aggtcatgct cgttggccgc ggtggtgact aggctgtggg tcaggccact
      421 cttggcatcg acaccaatgt gggccttcat gccaaagtgc cactgattgc ctttcttggt
      481 ctgatgcatc tccggatcgc gttgctgctc tttgttcttg gtagagctgg gtgcctcaat
      541 gatggtggca tccaccaaag tgccttgggt catcatgacg cctgcttcgg ccagccagcg
      601 attgatggtc ttgaacaatt gacgggccag ttgatgctgc tcgagcaggt ggcggaaatt
      661 catgatggtg gtgcgatccg gcagggcgct atccagggat aatcgggcaa acaggcgcat
      721 ggaggcgatt tcgtacaggg catcttccat ggcaccgtcg ctcaggttgt accaatgctg
      781 catgcagtga atacgcagca tggtctccag cggatagggc cgtcggccat tgcccgcctt
      841 gggataaaac ggctcgatga cttccaccat gttttgccat ggcagaatct gctccatgcg
      901 ggagaggaaa atctcttttc gggtctgacg gcgcttagtg ctgaattcac tatcggcgaa
      961 ggtgagttga tggctcatga tgtccctctg ggatgcgctc cggatgaata tgatgatctc
     1021 atatcaggaa cttgttcgca cctt
//
